pycom09g18830

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004012
Physical Location & Seq
Reverse (-)
19575471 .. 19578960
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2301 bp
ATGGCTTCTGGAGCAGCTATCAAACGCTCCGAGTCCATAGCTGATAACATGCCGGACGCCCTGAGGCAGAGCCGGTACCACATGAAGAGGTGCTTCGCTAAGTACATCGAGAAAGGAAGAAGGATAATAAAGCTTCACCATTTGTTGAGTGAAATGGAGACAGTCATAGATGACAAAGCTGAAAGAACCCAAGTTTTGGAGGGTGTTCTTGGCTATATATTATGTTCAACTCAGGAAGCTGTTGTTATTCCTCCACATGTTGTCTTTTCAATAAGACCAAATCCAGGATATTGGGAATTTGTTAAGGTCAGCTCTGAGGACCTCTCGGTTGAGGGCATCACTCTCAGAGACTTCTTGAAATACAAAGAAACTTTATATGATGAGAACTGGTCAAATGATGAAAATGCACTGGAGGTAGATTTTAGAGCAATTGATTTCTCTACTCCTCACTTAACTCTATCTTCCTCGATCGGAAATGGAATCAATTATGTTACCAAGTTCGCCACTTCAAAGCTAGCTGGAAAACTGGAGAATGCACAACCCCTTGTGGATTACTTACTTTCACTAAATCATCAAGGAGAACAACTTATTCTAAATGAGACCCTCAACACAGCTTCAAAGCTTCAGGCGATACTGATTGTAACAGAAGTGTACCTCTCAGCGCTTCCTAAGGACACACCATTCCAAAATTTTGAACTAAGGTTTAAGGAGTGGGGCTTTGAGAAGGGTTGGGGAGACACTGCAGAAAGAACAAAGGAGACAATGAGAATACTTTCGGAAGTACTACAGGCTCCAGACCCGTTGATCATGGACAGATTTTTCAGTAGGCTTCCCACAATATTCAATGTTGTAATATTCTCTCCTCATGGCTACTTTGGTCAAGCAGATGTTCTTGGCTTGCCAGACACTGGTGGGCAGGTAGTTTACATTCTTGATCAAGTGAAAGCTTTGGAGGAAGAATTGCTTGTGAGAATTAAGCAACAAGGACTTACTGTGAAGCCTCAAATTCTTGTGGTGACACGACTCATACCAGAAGCAAGGGGAACTAAGTGCAACCAGGAGTTGGAACCAATCAACGGCACAAAATACTCTAAGATTCTTAGGGTGCCATTTAGGACAGAGAAGGGAATCCTACGCCGTTGGGTTTCTCGTTTTGACATCTATCCCTATCTTGAATTGTTTACACAGGATGCTACTGCCAAAATCCTCGACCTGATGGAAGGAAAGCCAGATCTTATTATTGGAAACTACACAGATGGGAATTTGGTGGCGTCTCTCATGGCTAATAAACTTGGGATAACTCAGGCAACTATTGCTCATGCATTGGAGAAGACCAAGTACGAAGATTCAGACATCAACTGGAAGGAGTTAGATCCTAAGTATCACTTCTCATGCCAATTTCTTGCTGACACAATATCCATGAATGCTACAGATTTTGTCATTGCAAGCACATACCAGGAAATTGCAGGAAGCAAAGACAGACCGGGACAATATGAAAGCCACACGGCGTTTACGCTTCCGGGGCTCTGCAGAGTTGTTTCAGGCATCAGTGTATTTGATCCCAAATTCAACATTGCCGCTCCTGGGGCTGATCAGTCTGTCTATTTCCCCTACGCAGAGAAGCAGAAACGGCTCACTTCATTCCATCCTGCCATTGAAGAACTACTGCTTAGTAAAGAGGATAACAATGAACATATCGGATTTCTAGAGGACAGAAGGAAACCTATCATCTTCTCAATGGCAAGGCTGGACATAGTTAAAAACATTACTGGGTTGGTTGAGTGGTATGGGAAGAACAAGAGGCTGAGAAATTTGGTTAACCTTGTTGTAGTTGGGGGCTTCTTTGACCCTTCCAAATCAAAAGACAGAGAAGAAATTGCAGAAATAAAAAAGATGCATACACTGATAGAGAAGTACCAACTTAGGGATCAGATCAGATGGATAGCAGCGCAGACTGATAGGAACCGAAATGGAGAGCTCTACCGTTGTATTGCTGACACAAGGGGAGCTTTTGTGCAACCTGCTTTGTATGAAGCTTTCGGCCTGACTGTCATAGAGGCAATGAACTGTGGATTGCCCACCTTTGCAACCAACCAAGGAGGCCCGGCTGAAATCATTGTTGATGGGATCGATCTGGTTTCCATATTGATCCCAACAACGGTGACGAAGCAAGCAACAAAATCGCTGATTTCTTCGAGAAGTCTAAGACTGATGCTGCATACTGGGACAGATTTTCAAAAGCAGGCTTGCAGCGCATATACGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGA

Protein Analysis

767

Amino Acids

86.58

Weight (kDa)

6.33

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 3.5e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 246 5.7e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 269 - 556 2.3e-170 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 569 - 713 4.9e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 575 - 719 1.9e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 907, 2029
Acc65I GGTACC 1 cut(s) 75
AccB1I GGYRCC 2 cut(s) 75, 1105
AccB7I CCANNNNNTGG 3 cut(s) 196, 908, 1063
AccBSI CCGCTC 1 cut(s) 1580
AciI CCGC 1 cut(s) 1578
AclWI GGATC 5 cut(s) 1367, 1553, 1935, 2135, 2143
AcsI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 2 cut(s) 57, 1271
AfaI GTAC 6 cut(s) 77, 104, 653, 783, 1340, 1916
AfeI AGCGCT 1 cut(s) 663
AfiI CCNNNNNNNGG 8 cut(s) 196, 284, 908, 1063, 1076, 1584, 1924, 2158
AflIII ACRYGT 1 cut(s) 256
AjnI CCWGG 4 cut(s) 283, 1056, 1455, 1582
Alw21I GWGCWC 1 cut(s) 1980
Alw26I GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
AlwI GGATC 5 cut(s) 1367, 1553, 1935, 2135, 2143
AlwNI CAGNNNCTG 1 cut(s) 908
Aor51HI AGCGCT 1 cut(s) 663
AoxI GGCC 2 cut(s) 2041, 2101
ApeKI GCWGC 4 cut(s) 14, 1946, 2215, 2250
ApoI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
Asp700I GAANNNNTTC 1 cut(s) 772
Asp718I GGTACC 1 cut(s) 75
AspLEI GCGC 3 cut(s) 664, 1951, 2255
AspS9I GGNCC 2 cut(s) 319, 2102
AsuC2I CCSGG 3 cut(s) 1485, 1521, 2105
AsuHPI GGTGA 3 cut(s) 128, 1027, 2173
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 1 cut(s) 319
AxyI CCTNAGG 2 cut(s) 62, 669
BanI GGYRCC 2 cut(s) 75, 1105
BanII GRGCYC 2 cut(s) 1527, 1980
BarI GAAGNNNNNNTAC 2 cut(s) 1322, 1354
BbsI GAAGAC 1 cut(s) 1337
Bbv12I GWGCWC 1 cut(s) 1980
BbvI GCAGC 4 cut(s) 26, 1958, 2202, 2262
BccI CCATC 5 cut(s) 1210, 1250, 1653, 1932, 2117
BceAI ACGGC 4 cut(s) 1093, 1122, 1521, 1646
BcgI CGANNNNNNTGC 2 cut(s) 2163, 2197
BciT130I CCWGG 4 cut(s) 285, 1058, 1457, 1584
BclI TGATCA 3 cut(s) 804, 934, 1591
BcnI CCSGG 3 cut(s) 1485, 1521, 2105
BcoDI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BfaI CTAG 2 cut(s) 515, 1706
BfmI CTRYAG 4 cut(s) 741, 785, 1428, 1528
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 907, 2029
BglII AGATCT 1 cut(s) 1231
BisI GCNGC 5 cut(s) 15, 1578, 1947, 2216, 2251
BlsI GCNGC 5 cut(s) 16, 1579, 1948, 2217, 2252
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 319, 2102
BmiI GGNNCC 5 cut(s) 77, 792, 1068, 1107, 1964
BmrFI CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
BmrI ACTGGG 2 cut(s) 1779, 2232
BmsI GCATC 5 cut(s) 345, 1180, 1554, 1884, 2202
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 2 cut(s) 1779, 2232
BpiI GAAGAC 1 cut(s) 1337
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 4 cut(s) 30, 431, 548, 777
BpuMI CCSGG 3 cut(s) 1485, 1521, 2105
Bsa29I ATCGAT 1 cut(s) 2130
BsaHI GRCGYC 2 cut(s) 57, 1271
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 1520, 1583, 2095
BsaXI ACNNNNNCTCC 2 cut(s) 1998, 2028
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 284, 908, 1063, 1076, 1584, 1924, 2158
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 7 cut(s) 392, 414, 531, 913, 1364, 1774, 2227
Bse21I CCTNAGG 2 cut(s) 62, 669
Bse3DI GCAATG 3 cut(s) 1440, 1572, 2067
BseBI CCWGG 4 cut(s) 285, 1058, 1457, 1584
BseCI ATCGAT 1 cut(s) 2130
BseDI CCNNGG 3 cut(s) 1520, 1583, 2095
BseGI GGATG 2 cut(s) 1195, 1645
BseLI CCNNNNNNNGG 8 cut(s) 196, 284, 908, 1063, 1076, 1584, 1924, 2158
BseMI GCAATG 3 cut(s) 1440, 1572, 2067
BseMII CTCAG 7 cut(s) 53, 245, 306, 358, 672, 1316, 1796
BseNI ACTGG 7 cut(s) 392, 414, 531, 913, 1364, 1774, 2227
BseRI GAGGAG 2 cut(s) 435, 852
BseXI GCAGC 4 cut(s) 26, 1958, 2202, 2262
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 2043, 2103
BshNI GGYRCC 2 cut(s) 75, 1105
BshVI ATCGAT 1 cut(s) 2130
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 1980
BsiSI CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
BslFI GGGAC 2 cut(s) 1500, 2239
BslI CCNNNNNNNGG 8 cut(s) 196, 284, 908, 1063, 1076, 1584, 1924, 2158
BsmAI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 2 cut(s) 1500, 2239
BsmI GAATGC 3 cut(s) 538, 1429, 2269
BsnI GGCC 2 cut(s) 2043, 2103
Bso31I GGTCTC 1 cut(s) 593
Bsp1286I GDGCHC 2 cut(s) 1527, 1980
BspACI CCGC 1 cut(s) 1578
BspANI GGCC 2 cut(s) 2043, 2103
BspCNI CTCAG 7 cut(s) 54, 244, 307, 357, 671, 1315, 1797
BspDI ATCGAT 1 cut(s) 2130
BspLI GGNNCC 5 cut(s) 77, 792, 1068, 1107, 1964
BspMAI CTGCAG 2 cut(s) 745, 1532
BspMI ACCTGC 2 cut(s) 907, 2029
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 5 cut(s) 1367, 1553, 1935, 2135, 2143
BspT107I GGYRCC 2 cut(s) 75, 1105
BspTNI GGTCTC 1 cut(s) 593
BsrBI CCGCTC 1 cut(s) 1580
BsrDI GCAATG 3 cut(s) 1440, 1572, 2067
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 7 cut(s) 392, 414, 531, 913, 1364, 1774, 2227
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 1520, 1583, 2095
BssNI GRCGYC 2 cut(s) 57, 1271
BssT1I CCWWGG 1 cut(s) 2095
Bst2UI CCWGG 4 cut(s) 285, 1058, 1457, 1584
Bst4CI ACNGT 6 cut(s) 163, 994, 1985, 2050, 2069, 2161
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 57, 1271
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstC8I GCNNGC 6 cut(s) 516, 899, 1447, 2172, 2244, 2248
BstF5I GGATG 2 cut(s) 1195, 1645
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 3 cut(s) 664, 1951, 2255
BstMAI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 6 cut(s) 11, 1313, 1522, 1586, 1630, 2252
BstNI CCWGG 4 cut(s) 285, 1058, 1457, 1584
BstNSI RCATGY 2 cut(s) 52, 260
BstSCI CCNGG 7 cut(s) 283, 1056, 1455, 1483, 1519, 1582, 2103
BstSFI CTRYAG 4 cut(s) 741, 785, 1428, 1528
BstV1I GCAGC 4 cut(s) 26, 1958, 2202, 2262
BstV2I GAAGAC 1 cut(s) 1337
BstX2I RGATCY 2 cut(s) 1231, 1372
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 2 cut(s) 1231, 1372
Bsu15I ATCGAT 1 cut(s) 2130
Bsu36I CCTNAGG 2 cut(s) 62, 669
BsuRI GGCC 2 cut(s) 2043, 2103
BsuTUI ATCGAT 1 cut(s) 2130
BtsCI GGATG 2 cut(s) 1195, 1645
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 5 cut(s) 407, 738, 906, 1555, 1901
BveI ACCTGC 2 cut(s) 907, 2029
Cac8I GCNNGC 6 cut(s) 516, 899, 1447, 2172, 2244, 2248
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 3 cut(s) 664, 1951, 2255
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 319, 2102
ClaI ATCGAT 1 cut(s) 2130
CseI GACGC 2 cut(s) 65, 1260
Csp6I GTAC 6 cut(s) 76, 103, 652, 782, 1339, 1915
CviAII CATG 9 cut(s) 49, 82, 257, 808, 866, 1279, 1319, 1392, 1420
CviQI GTAC 6 cut(s) 76, 103, 652, 782, 1339, 1915
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 1978
Eco130I CCWWGG 1 cut(s) 2095
Eco24I GRGCYC 2 cut(s) 1527, 1980
Eco31I GGTCTC 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 1978
Eco57I CTGAAG 1 cut(s) 608
Eco81I CCTNAGG 2 cut(s) 62, 669
EcoICRI GAGCTC 1 cut(s) 1978
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 4 cut(s) 283, 1056, 1455, 1582
EcoT14I CCWWGG 1 cut(s) 2095
EcoT22I ATGCAT 2 cut(s) 1324, 1899
EcoT38I GRGCYC 2 cut(s) 1527, 1980
ErhI CCWWGG 1 cut(s) 2095
Esp3I CGTCTC 1 cut(s) 1278
FaeI CATG 9 cut(s) 52, 85, 260, 811, 869, 1282, 1322, 1395, 1423
FalI AAGNNNNNCTT 8 cut(s) 77, 109, 948, 980, 1370, 1402, 1993, 2025
FaqI GGGAC 2 cut(s) 1500, 2239
FatI CATG 9 cut(s) 48, 81, 256, 807, 865, 1278, 1318, 1391, 1419
FbaI TGATCA 3 cut(s) 804, 934, 1591
Fnu4HI GCNGC 5 cut(s) 15, 1578, 1947, 2216, 2251
FokI GGATG 2 cut(s) 1202, 1632
FriOI GRGCYC 2 cut(s) 1527, 1980
Fsp4HI GCNGC 5 cut(s) 15, 1578, 1947, 2216, 2251
FspBI CTAG 2 cut(s) 515, 1706
GlaI GCGC 3 cut(s) 663, 1950, 2254
GluI GCNGC 5 cut(s) 15, 1578, 1947, 2216, 2251
GsuI CTGGAG 4 cut(s) 30, 431, 548, 777
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 2 cut(s) 2043, 2103
HapII CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
HgaI GACGC 2 cut(s) 65, 1260
HhaI GCGC 3 cut(s) 664, 1951, 2255
Hin1I GRCGYC 2 cut(s) 57, 1271
Hin1II CATG 9 cut(s) 52, 85, 260, 811, 869, 1282, 1322, 1395, 1423
Hin6I GCGC 3 cut(s) 662, 1949, 2253
HinP1I GCGC 3 cut(s) 662, 1949, 2253
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HindIII AAGCTT 4 cut(s) 131, 620, 945, 2034
HinfI GANTC 6 cut(s) 32, 480, 1023, 1096, 1128, 1346
HpaI GTTAAC 1 cut(s) 1819
HpaII CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
HphI GGTGA 3 cut(s) 128, 1027, 2173
Hpy166II GTNNAC 5 cut(s) 652, 925, 1182, 1512, 1819
Hpy188I TCNGA 9 cut(s) 31, 316, 347, 473, 778, 1351, 1700, 1932, 1937
Hpy188III TCNNGA 9 cut(s) 9, 109, 233, 355, 794, 932, 1172, 1706, 2196
Hpy8I GTNNAC 5 cut(s) 652, 925, 1182, 1512, 1819
HpyAV CCTTC 7 cut(s) 114, 718, 1117, 1214, 1357, 1710, 1860
HpyCH4III ACNGT 6 cut(s) 163, 994, 1985, 2050, 2069, 2161
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 1313, 1522, 1586, 1630, 2252
Hsp92I GRCGYC 2 cut(s) 57, 1271
Hsp92II CATG 9 cut(s) 52, 85, 260, 811, 869, 1282, 1322, 1395, 1423
HspAI GCGC 3 cut(s) 662, 1949, 2253
KpnI GGTACC 1 cut(s) 79
Ksp22I TGATCA 3 cut(s) 804, 934, 1591
KspAI GTTAAC 1 cut(s) 1819
LmnI GCTCC 5 cut(s) 11, 32, 796, 1585, 2006
Lsp1109I GCAGC 4 cut(s) 26, 1958, 2202, 2262
LweI GCATC 5 cut(s) 345, 1180, 1554, 1884, 2202
MaeI CTAG 2 cut(s) 515, 1706
MaeIII GTNAC 4 cut(s) 490, 640, 1015, 2161
MbiI CCGCTC 1 cut(s) 1580
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 2 cut(s) 1231, 1372
MhlI GDGCHC 2 cut(s) 1527, 1980
MlyI GAGTC 2 cut(s) 41, 1017
MmeI TCCRAC 1 cut(s) 1044
Mph1103I ATGCAT 2 cut(s) 1324, 1899
MroXI GAANNNNTTC 1 cut(s) 772
MseI TTAA 6 cut(s) 303, 452, 705, 975, 1758, 1818
MspI CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
MspR9I CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 3 cut(s) 538, 1429, 2269
MvaI CCWGG 4 cut(s) 285, 1058, 1457, 1584
MwoI GCNNNNNNNGC 6 cut(s) 11, 1313, 1522, 1586, 1630, 2252
NciI CCSGG 3 cut(s) 1485, 1521, 2105
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 9 cut(s) 52, 85, 260, 811, 869, 1282, 1322, 1395, 1423
NlaIV GGNNCC 5 cut(s) 77, 792, 1068, 1107, 1964
NmuCI GTSAC 2 cut(s) 1015, 2161
NsiI ATGCAT 2 cut(s) 1324, 1899
NspI RCATGY 2 cut(s) 52, 260
PciI ACATGT 1 cut(s) 256
PctI GAATGC 3 cut(s) 538, 1429, 2269
PdmI GAANNNNTTC 1 cut(s) 772
PfeI GAWTC 4 cut(s) 480, 1096, 1128, 1346
PflMI CCANNNNNTGG 3 cut(s) 196, 908, 1063
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 5 cut(s) 16, 1579, 1948, 2217, 2252
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 2 cut(s) 40, 1017
PpsI GAGTC 2 cut(s) 40, 1017
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 1980
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 4 cut(s) 283, 1056, 1455, 1582
PspGI CCWGG 4 cut(s) 283, 1056, 1455, 1582
PspN4I GGNNCC 5 cut(s) 77, 792, 1068, 1107, 1964
PspPI GGNCC 2 cut(s) 319, 2102
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 2 cut(s) 745, 1532
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 2 cut(s) 1231, 1372
PvuI CGATCG 1 cut(s) 471
RsaI GTAC 6 cut(s) 77, 104, 653, 783, 1340, 1916
RsaNI GTAC 6 cut(s) 76, 103, 652, 782, 1339, 1915
SacI GAGCTC 1 cut(s) 1980
SaqAI TTAA 6 cut(s) 303, 452, 705, 975, 1758, 1818
SatI GCNGC 5 cut(s) 15, 1578, 1947, 2216, 2251
Sau96I GGNCC 2 cut(s) 319, 2102
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 2 cut(s) 41, 1017
ScrFI CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
SduI GDGCHC 2 cut(s) 1527, 1980
SfaNI GCATC 5 cut(s) 345, 1180, 1554, 1884, 2202
SfcI CTRYAG 4 cut(s) 741, 785, 1428, 1528
SinI GGWCC 1 cut(s) 319
SsiI CCGC 1 cut(s) 1578
SspI AATATT 2 cut(s) 840, 855
SspMI CTAG 2 cut(s) 515, 1706
SstI GAGCTC 1 cut(s) 1980
StyD4I CCNGG 7 cut(s) 283, 1056, 1455, 1483, 1519, 1582, 2103
StyI CCWWGG 1 cut(s) 2095
TaaI ACNGT 6 cut(s) 163, 994, 1985, 2050, 2069, 2161
TaqI TCGA 5 cut(s) 108, 467, 1209, 2130, 2195
TatI WGTACW 2 cut(s) 102, 781
TauI GCSGC 1 cut(s) 1580
TfiI GAWTC 4 cut(s) 480, 1096, 1128, 1346
Tru1I TTAA 6 cut(s) 303, 452, 705, 975, 1758, 1818
Tru9I TTAA 6 cut(s) 303, 452, 705, 975, 1758, 1818
TscAI CASTG 5 cut(s) 414, 745, 913, 1555, 1908
TseFI GTSAC 2 cut(s) 1015, 2161
TseI GCWGC 4 cut(s) 14, 1946, 2215, 2250
Tsp45I GTSAC 2 cut(s) 1015, 2161
TspDTI ATGAA 8 cut(s) 98, 414, 1436, 1509, 1629, 1704, 2046, 2078
TspRI CASTG 5 cut(s) 414, 745, 913, 1555, 1908
Van91I CCANNNNNTGG 3 cut(s) 196, 908, 1063
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
XbaI TCTAGA 1 cut(s) 1705
XceI RCATGY 2 cut(s) 52, 260
XmnI GAANNNNTTC 1 cut(s) 772
XspI CTAG 2 cut(s) 515, 1706
ZrmI AGTACT 1 cut(s) 783
Zsp2I ATGCAT 2 cut(s) 1324, 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.