pycom10g01610

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012146
Physical Location & Seq
Forward (+)
1688677 .. 1696691
8015 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1866 bp
ATGGCGGATCACGAGGAGGAGGACCTCCGAATGGCACTGCGGATGAGTATGCAGAATTCACCTCCGGAGCCCAAGCGTAGCAAGCCCAGGGACGGACCCGCCGGAGCTCTGGCTGAGGAGCCGTTGGAAGTGAAGACCCGGAGACTTCAGCGGGAGCTCATGGCTGCTGCTGCTGAGAAGAGAATGCTGGACACCAATACGTGTCCGCCTTCGGCTTCGGCTTCACCTAGCAAAGTGTTGTCGGGGTCTGCGGGTTCCGCTTCGGGTTTGGCTCCAAAGGTGGCGCCGAAAGATAAGGAATTGAGCTTGGAGGAAGTGAATTTGGGAAAGGGGTTGAGCGAAGAGGATGCAAAGCAGCTGTTTTCGATGGTTTTCGGGCCCGAGGTTACAAAGGGCATTCTTGTGCAGTGGAGTAATCAGGGAATAAGGTTTAGCTCTGATCCAGAAACATCTATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCATTTGTTCTCAAATACCTCCTTTTCTACCCAGCTGAATTAGGTAAAGTTGCACCAAACATGCATCAAAATACGGGTTCAAGGACACCGTCTAATAGTCAGTGTGTTGCATCGAATAATTTTGCCTCTCTTACTGAAGATGCAAAAGCAAGCGTACTTATTAGAAGTATGGGTGAGATATTGTTCTTATGTGGAAGCAATAAAAGGGCGGTGATTGCAACTTTGAGTGTTATTGGAGATGAAACTGAGAGGGCTGACGACAACCTGAATGATGAAGTAATAACAAAGTCACTTGAAGGTCTTTCAATCGAATCAGCTGCTGATTTGCATAAAGTGCTAAGAGTTAGCACTTACACAACACAAGAAAGTGCGTTTATGGGGCTCCAAGCAGTGCTTCCTGTCTTCCAAAGTCGTATGGGGGCACTGCTTTTCCTTATTTCTGCTTTACTTTCTCGAGGACTGGACTTGGTACAATCTGACAGGGATGACCCCGGCCTACCTCTTGTAACTGCTCCATTTGGGCATGCTTCACAGGAAATTGTGAACTTATTGCTCTGCGGGCAGGCAGTCCCTAATGTTTTTGATGGAAAGATGGATTTAGGTGGTGGCATGTCTTTAAAGGGCATATCCAAAACTGTGGAAGTTGGATTCCTCACTCTGTTAGAATCCCTAAATTTCTGTAAGGTTGGTCAGTATTTGAAAAGTCCAAAATGGCCAATCTGGGTAGTTGGGAGTGAGTCTCATTATACAGTCCTTTTTTCTCTTGATACCACTGTCCAAGATGAGAATGAATTGGAAGGAAGGGAATCACAGATTCGGAGAGCTTTTGATGCACAAGATCAGAGTGGCGGTGGTGGTTTTATCAGCGTGGAAGGCTTTCATCAAGTCCTTAGGGAAACTAACGTAAAACTTCCAACTGAGAAGGTTGACCTCCTCTGCAACACAGGATTTATTGTATGGAGTGAGTTGTGGCAGGTCATTTTGGATTTGGACAAGAGCTTGGGAGGCCTGAAGGATTCAACTGGATTGATGGGTAAGAAGGTGTTTGATCTTTACCATTTTAATGGAATTGCAAAATCTGACCTGAATGGAAGCCAGACAACCTCTGGAGGCGATATGCCAGTTCAAAGACCTAGGCTCACAAAATTGAGGGTGTCGGTACCTCCGAGGTGGACTCCTGAAGAATACATGACAGATGTGGTAGTGTCCTCTGCTTCTAGTGGTAATGAATCTGGAGGGAAAGTCACAGAAATCGCTAAGCCGGAGCCTGCTCAGCATGCGCCTCTGGTGGACTGCATAAGAACGCGCTGGCCCCGTGCCGTCTGCAATTGGGTAGGTGACCCGCCTAGCATAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

622

Amino Acids

67.13

Weight (kDa)

5.27

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 123 - 433 3e-59 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1471
Acc65I GGTACC 1 cut(s) 1666
AccB1I GGYRCC 2 cut(s) 283, 1666
AccII CGCG 1 cut(s) 1813
AccIII TCCGGA 1 cut(s) 64
AclWI GGATC 2 cut(s) 15, 434
AcoI YGGCCR 1 cut(s) 1220
AcsI RAATTY 3 cut(s) 55, 319, 1180
AcuI CTGAAG 4 cut(s) 131, 663, 1538, 1707
AcyI GRCGYC 1 cut(s) 284
AfaI GTAC 3 cut(s) 663, 978, 1668
AfiI CCNNNNNNNGG 2 cut(s) 31, 92
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 7 cut(s) 506, 588, 803, 813, 1207, 1527, 1634
AjnI CCWGG 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 885, 917
AluBI AGCT 9 cut(s) 107, 157, 306, 358, 435, 542, 824, 1331, 1506
AluI AGCT 9 cut(s) 107, 157, 306, 358, 435, 542, 824, 1331, 1506
Alw21I GWGCWC 2 cut(s) 109, 159
Alw26I GTCTC 2 cut(s) 136, 1251
AlwI GGATC 2 cut(s) 15, 434
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 2 cut(s) 380, 960
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 6 cut(s) 377, 479, 1000, 1220, 1513, 1817
ApaI GGGCCC 2 cut(s) 381, 483
ApeKI GCWGC 7 cut(s) 164, 167, 170, 355, 466, 497, 824
ApoI RAATTY 3 cut(s) 55, 319, 1180
Asp700I GAANNNNTTC 1 cut(s) 1383
Asp718I GGTACC 1 cut(s) 1666
AspA2I CCTAGG 1 cut(s) 1640
AspLEI GCGC 3 cut(s) 286, 1789, 1815
AspS9I GGNCC 7 cut(s) 22, 95, 377, 378, 479, 480, 1818
AsuC2I CCSGG 2 cut(s) 139, 999
AsuHPI GGTGA 5 cut(s) 51, 216, 692, 730, 1856
AvaI CYCGRG 2 cut(s) 380, 960
AvaII GGWCC 2 cut(s) 22, 95
AvrII CCTAGG 1 cut(s) 1640
AxyI CCTNAGG 1 cut(s) 1397
BaeGI GKGCMC 3 cut(s) 381, 483, 931
BaeI ACNNNNGTAYC 2 cut(s) 1266, 1299
BalI TGGCCA 1 cut(s) 1222
BanI GGYRCC 2 cut(s) 283, 1666
BanII GRGCYC 6 cut(s) 72, 109, 159, 381, 483, 891
BauI CACGAG 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 140, 901
Bbv12I GWGCWC 2 cut(s) 109, 159
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 7 cut(s) 151, 154, 157, 367, 478, 509, 811
BccI CCATC 4 cut(s) 361, 1085, 1093, 1531
BceAI ACGGC 2 cut(s) 106, 1811
BcgI CGANNNNNNTGC 4 cut(s) 329, 363, 806, 840
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 2 cut(s) 139, 999
BcoDI GTCTC 2 cut(s) 136, 1251
BfaI CTAG 5 cut(s) 228, 461, 1641, 1725, 1854
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 1471
BisI GCNGC 7 cut(s) 165, 168, 171, 356, 467, 498, 825
BlnI CCTAGG 1 cut(s) 1640
BlpI GCTNAGC 2 cut(s) 1764, 1779
BlsI GCNGC 7 cut(s) 166, 169, 172, 357, 468, 499, 826
Bme1390I CCNGG 3 cut(s) 88, 139, 999
Bme18I GGWCC 2 cut(s) 22, 95
BmeT110I CYCGRG 2 cut(s) 380, 960
BmgT120I GGNCC 7 cut(s) 22, 95, 377, 378, 479, 480, 1818
BmrFI CCNGG 3 cut(s) 88, 139, 999
BmsI GCATC 5 cut(s) 337, 580, 626, 637, 1327
BpiI GAAGAC 2 cut(s) 140, 901
BplI GAGNNNNNCTC 4 cut(s) 1231, 1263, 1666, 1698
BpmI CTGGAG 2 cut(s) 1635, 1761
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 2 cut(s) 1764, 1779
BpuMI CCSGG 2 cut(s) 139, 999
BsaAI YACGTR 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 284
BsaJI CCNNGG 6 cut(s) 86, 87, 381, 997, 1640, 1673
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 1734, 1764
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 92
Bse1I ACTGG 3 cut(s) 972, 1534, 1628
Bse21I CCTNAGG 1 cut(s) 1397
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 6 cut(s) 86, 87, 381, 997, 1640, 1673
BseGI GGATG 3 cut(s) 48, 352, 997
BseLI CCNNNNNNNGG 2 cut(s) 31, 92
BseMII CTCAG 5 cut(s) 105, 165, 744, 1416, 1793
BseNI ACTGG 3 cut(s) 972, 1534, 1628
BseRI GAGGAG 4 cut(s) 29, 32, 131, 1430
BseSI GKGCMC 3 cut(s) 381, 483, 931
BseXI GCAGC 7 cut(s) 151, 154, 157, 367, 478, 509, 811
BseYI CCCAGC 1 cut(s) 538
BsgI GTGCAG 2 cut(s) 425, 485
Bsh1236I CGCG 1 cut(s) 1813
BshFI GGCC 6 cut(s) 379, 481, 1002, 1222, 1515, 1819
BshNI GGYRCC 2 cut(s) 283, 1666
BsiHKAI GWGCWC 2 cut(s) 109, 159
BsiHKCI CYCGRG 2 cut(s) 380, 960
BsiSI CCGG 5 cut(s) 65, 102, 139, 999, 1769
BslFI GGGAC 2 cut(s) 104, 1061
BslI CCNNNNNNNGG 2 cut(s) 31, 92
BsmAI GTCTC 2 cut(s) 136, 1251
BsmFI GGGAC 2 cut(s) 104, 1061
BsmI GAATGC 2 cut(s) 189, 396
BsnI GGCC 6 cut(s) 379, 481, 1002, 1222, 1515, 1819
BsoBI CYCGRG 2 cut(s) 380, 960
Bsp120I GGGCCC 2 cut(s) 377, 479
Bsp1286I GDGCHC 7 cut(s) 72, 109, 159, 381, 483, 891, 931
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 4 cut(s) 7, 439, 1345, 1555
Bsp1720I GCTNAGC 2 cut(s) 1764, 1779
BspANI GGCC 6 cut(s) 379, 481, 1002, 1222, 1515, 1819
BspCNI CTCAG 5 cut(s) 106, 166, 745, 1417, 1792
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 1 cut(s) 1813
BspMI ACCTGC 1 cut(s) 1471
BspPI GGATC 2 cut(s) 15, 434
BspT107I GGYRCC 2 cut(s) 283, 1666
BsrI ACTGG 3 cut(s) 972, 1534, 1628
BssECI CCNNGG 6 cut(s) 86, 87, 381, 997, 1640, 1673
BssMI GATC 4 cut(s) 7, 439, 1345, 1555
BssNI GRCGYC 1 cut(s) 284
BssSI CACGAG 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 1640
Bst2BI CACGAG 1 cut(s) 11
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 4 cut(s) 597, 1144, 1258, 1282
Bst6I CTCTTC 2 cut(s) 173, 336
BstACI GRCGYC 1 cut(s) 284
BstAPI GCANNNNNTGC 1 cut(s) 841
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 8 cut(s) 83, 658, 1032, 1067, 1071, 1776, 1785, 1817
BstDEI CTNAG 8 cut(s) 114, 174, 753, 845, 1397, 1425, 1764, 1779
BstEII GGTNACC 1 cut(s) 1844
BstF5I GGATG 3 cut(s) 48, 352, 997
BstFNI CGCG 1 cut(s) 1813
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 3 cut(s) 286, 1789, 1815
BstKTI GATC 4 cut(s) 10, 442, 1348, 1558
BstMAI GTCTC 2 cut(s) 136, 1251
BstMBI GATC 4 cut(s) 7, 439, 1345, 1555
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 4 cut(s) 571, 1034, 1120, 1787
BstPI GGTNACC 1 cut(s) 1844
BstSCI CCNGG 3 cut(s) 86, 137, 997
BstSLI GKGCMC 3 cut(s) 381, 483, 931
BstUI CGCG 1 cut(s) 1813
BstV1I GCAGC 7 cut(s) 151, 154, 157, 367, 478, 509, 811
BstV2I GAAGAC 2 cut(s) 140, 901
BstXI CCANNNNNNTGG 2 cut(s) 1144, 1571
Bsu36I CCTNAGG 1 cut(s) 1397
BsuRI GGCC 6 cut(s) 379, 481, 1002, 1222, 1515, 1819
BtsCI GGATG 3 cut(s) 48, 352, 997
BtsI GCAGTG 4 cut(s) 35, 413, 903, 929
BtsIMutI CAGTG 6 cut(s) 35, 413, 614, 903, 929, 1278
BveI ACCTGC 1 cut(s) 1471
Cac8I GCNNGC 8 cut(s) 83, 658, 1032, 1067, 1071, 1776, 1785, 1817
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 3 cut(s) 286, 1789, 1815
Cfr13I GGNCC 7 cut(s) 22, 95, 377, 378, 479, 480, 1818
Csp6I GTAC 3 cut(s) 662, 977, 1667
CviAII CATG 7 cut(s) 160, 470, 568, 1031, 1117, 1696, 1784
CviQI GTAC 3 cut(s) 662, 977, 1667
DdeI CTNAG 8 cut(s) 114, 174, 753, 845, 1397, 1425, 1764, 1779
DinI GGCGCC 1 cut(s) 285
DpnI GATC 4 cut(s) 9, 441, 1347, 1557
DpnII GATC 4 cut(s) 7, 439, 1345, 1555
DraI TTTAAA 1 cut(s) 1125
EaeI YGGCCR 1 cut(s) 1220
Eam1104I CTCTTC 2 cut(s) 173, 336
EarI CTCTTC 2 cut(s) 173, 336
EciI GGCGGA 2 cut(s) 20, 195
Ecl136II GAGCTC 2 cut(s) 107, 157
Eco130I CCWWGG 1 cut(s) 1640
Eco147I AGGCCT 1 cut(s) 1515
Eco24I GRGCYC 6 cut(s) 72, 109, 159, 381, 483, 891
Eco47I GGWCC 2 cut(s) 22, 95
Eco53kI GAGCTC 2 cut(s) 107, 157
Eco57I CTGAAG 4 cut(s) 131, 663, 1538, 1707
Eco81I CCTNAGG 1 cut(s) 1397
Eco88I CYCGRG 2 cut(s) 380, 960
Eco91I GGTNACC 1 cut(s) 1844
EcoICRI GAGCTC 2 cut(s) 107, 157
EcoO109I RGGNCCY 2 cut(s) 22, 480
EcoO65I GGTNACC 1 cut(s) 1844
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 1640
EcoT22I ATGCAT 1 cut(s) 573
EcoT38I GRGCYC 6 cut(s) 72, 109, 159, 381, 483, 891
EgeI GGCGCC 1 cut(s) 285
EheI GGCGCC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 1640
FaeI CATG 7 cut(s) 163, 473, 571, 1034, 1120, 1699, 1787
FalI AAGNNNNNCTT 2 cut(s) 885, 917
FaqI GGGAC 2 cut(s) 104, 1061
FatI CATG 7 cut(s) 159, 469, 567, 1030, 1116, 1695, 1783
FauI CCCGC 5 cut(s) 106, 144, 244, 1058, 1857
Fnu4HI GCNGC 7 cut(s) 165, 168, 171, 356, 467, 498, 825
FokI GGATG 3 cut(s) 55, 359, 1004
FriOI GRGCYC 6 cut(s) 72, 109, 159, 381, 483, 891
Fsp4HI GCNGC 7 cut(s) 165, 168, 171, 356, 467, 498, 825
FspBI CTAG 5 cut(s) 228, 461, 1641, 1725, 1854
GlaI GCGC 3 cut(s) 285, 1788, 1814
GluI GCNGC 7 cut(s) 165, 168, 171, 356, 467, 498, 825
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 2 cut(s) 1635, 1761
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 6 cut(s) 379, 481, 1002, 1222, 1515, 1819
HapII CCGG 5 cut(s) 65, 102, 139, 999, 1769
HhaI GCGC 3 cut(s) 286, 1789, 1815
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 7 cut(s) 163, 473, 571, 1034, 1120, 1699, 1787
Hin6I GCGC 3 cut(s) 284, 1787, 1813
HinP1I GCGC 3 cut(s) 284, 1787, 1813
HincII GTYRAC 1 cut(s) 1435
HindII GTYRAC 1 cut(s) 1435
HinfI GANTC 9 cut(s) 818, 1155, 1172, 1244, 1313, 1321, 1523, 1681, 1736
HpaII CCGG 5 cut(s) 65, 102, 139, 999, 1769
HphI GGTGA 5 cut(s) 51, 216, 692, 730, 1856
Hpy166II GTNNAC 4 cut(s) 1051, 1435, 1680, 1798
Hpy188I TCNGA 7 cut(s) 29, 439, 985, 1326, 1350, 1588, 1674
Hpy188III TCNNGA 8 cut(s) 11, 65, 443, 960, 1271, 1614, 1685, 1740
Hpy8I GTNNAC 4 cut(s) 1051, 1435, 1680, 1798
HpyAV CCTTC 9 cut(s) 219, 467, 797, 1298, 1302, 1373, 1423, 1513, 1540
HpyCH4III ACNGT 4 cut(s) 597, 1144, 1258, 1282
HpyCH4IV ACGT 2 cut(s) 200, 1410
HpyF3I CTNAG 8 cut(s) 114, 174, 753, 845, 1397, 1425, 1764, 1779
HpySE526I ACGT 2 cut(s) 200, 1410
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 7 cut(s) 163, 473, 571, 1034, 1120, 1699, 1787
HspAI GCGC 3 cut(s) 284, 1787, 1813
KasI GGCGCC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 64
KpnI GGTACC 1 cut(s) 1670
Kzo9I GATC 4 cut(s) 7, 439, 1345, 1555
LmnI GCTCC 8 cut(s) 67, 104, 118, 154, 277, 894, 1024, 1771
Lsp1109I GCAGC 7 cut(s) 151, 154, 157, 367, 478, 509, 811
LweI GCATC 5 cut(s) 337, 580, 626, 637, 1327
MaeI CTAG 5 cut(s) 228, 461, 1641, 1725, 1854
MaeII ACGT 2 cut(s) 200, 1410
MaeIII GTNAC 5 cut(s) 385, 795, 1012, 1750, 1844
MalI GATC 4 cut(s) 9, 441, 1347, 1557
MboI GATC 4 cut(s) 7, 439, 1345, 1555
MboII GAAGA 6 cut(s) 145, 190, 353, 656, 901, 1700
MfeI CAATTG 1 cut(s) 1834
MhlI GDGCHC 7 cut(s) 72, 109, 159, 381, 483, 891, 931
MlsI TGGCCA 1 cut(s) 1222
MluNI TGGCCA 1 cut(s) 1222
Mly113I GGCGCC 1 cut(s) 284
MlyI GAGTC 2 cut(s) 1253, 1675
MmeI TCCRAC 3 cut(s) 105, 1132, 1445
Mox20I TGGCCA 1 cut(s) 1222
Mph1103I ATGCAT 1 cut(s) 573
MroI TCCGGA 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 1383
MscI TGGCCA 1 cut(s) 1222
MseI TTAA 2 cut(s) 1124, 1569
MslI CAYNNNNRTG 3 cut(s) 401, 474, 1569
Msp20I TGGCCA 1 cut(s) 1222
MspA1I CMGCKG 4 cut(s) 151, 358, 542, 824
MspI CCGG 5 cut(s) 65, 102, 139, 999, 1769
MspR9I CCNGG 3 cut(s) 88, 139, 999
MunI CAATTG 1 cut(s) 1834
Mva1269I GAATGC 2 cut(s) 189, 396
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 1813
NarI GGCGCC 1 cut(s) 284
NciI CCSGG 2 cut(s) 139, 999
NdeII GATC 4 cut(s) 7, 439, 1345, 1555
NlaIII CATG 7 cut(s) 163, 473, 571, 1034, 1120, 1699, 1787
NmuCI GTSAC 3 cut(s) 795, 1750, 1844
NsiI ATGCAT 1 cut(s) 573
NspI RCATGY 4 cut(s) 571, 1034, 1120, 1787
PaeI GCATGC 2 cut(s) 1034, 1787
PaeR7I CTCGAG 1 cut(s) 960
PasI CCCWGGG 1 cut(s) 87
PceI AGGCCT 1 cut(s) 1515
PcsI WCGNNNNNNNCGW 1 cut(s) 1670
PctI GAATGC 2 cut(s) 189, 396
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 7 cut(s) 818, 1155, 1172, 1313, 1321, 1523, 1736
PflFI GACNNNGTC 1 cut(s) 595
PkrI GCNGC 7 cut(s) 166, 169, 172, 357, 468, 499, 826
PleI GAGTC 2 cut(s) 1252, 1675
PluTI GGCGCC 1 cut(s) 287
PpsI GAGTC 2 cut(s) 1252, 1675
Ppu21I YACGTR 1 cut(s) 201
PpuMI RGGWCCY 1 cut(s) 22
Psp124BI GAGCTC 2 cut(s) 109, 159
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 1844
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 1 cut(s) 86
PspOMI GGGCCC 2 cut(s) 377, 479
PspPI GGNCC 7 cut(s) 22, 95, 377, 378, 479, 480, 1818
PspPPI RGGWCCY 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 827
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 3 cut(s) 358, 542, 824
RsaI GTAC 3 cut(s) 663, 978, 1668
RsaNI GTAC 3 cut(s) 662, 977, 1667
RseI CAYNNNNRTG 3 cut(s) 401, 474, 1569
SacI GAGCTC 2 cut(s) 109, 159
SaqAI TTAA 2 cut(s) 1124, 1569
SatI GCNGC 7 cut(s) 165, 168, 171, 356, 467, 498, 825
Sau3AI GATC 4 cut(s) 7, 439, 1345, 1555
Sau96I GGNCC 7 cut(s) 22, 95, 377, 378, 479, 480, 1818
SchI GAGTC 2 cut(s) 1253, 1675
ScrFI CCNGG 3 cut(s) 88, 139, 999
SduI GDGCHC 7 cut(s) 72, 109, 159, 381, 483, 891, 931
SfaNI GCATC 5 cut(s) 337, 580, 626, 637, 1327
SfoI GGCGCC 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 960
SinI GGWCC 2 cut(s) 22, 95
SlaI CTCGAG 1 cut(s) 960
SmiMI CAYNNNNRTG 3 cut(s) 401, 474, 1569
SmlI CTYRAG 1 cut(s) 960
SmoI CTYRAG 1 cut(s) 960
SphI GCATGC 2 cut(s) 1034, 1787
SseBI AGGCCT 1 cut(s) 1515
SspDI GGCGCC 1 cut(s) 283
SspMI CTAG 5 cut(s) 228, 461, 1641, 1725, 1854
SstI GAGCTC 2 cut(s) 109, 159
StuI AGGCCT 1 cut(s) 1515
StyD4I CCNGG 3 cut(s) 86, 137, 997
StyI CCWWGG 1 cut(s) 1640
TaaI ACNGT 4 cut(s) 597, 1144, 1258, 1282
TaiI ACGT 2 cut(s) 203, 1413
TaqI TCGA 4 cut(s) 365, 620, 816, 961
TfiI GAWTC 7 cut(s) 818, 1155, 1172, 1313, 1321, 1523, 1736
Tru1I TTAA 2 cut(s) 1124, 1569
Tru9I TTAA 2 cut(s) 1124, 1569
TscAI CASTG 6 cut(s) 42, 413, 614, 903, 936, 1285
TseFI GTSAC 3 cut(s) 795, 1750, 1844
TseI GCWGC 7 cut(s) 164, 167, 170, 355, 466, 497, 824
Tsp45I GTSAC 3 cut(s) 795, 1750, 1844
TspDTI ATGAA 6 cut(s) 486, 762, 795, 1311, 1376, 1749
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 6 cut(s) 42, 413, 614, 903, 936, 1285
Tth111I GACNNNGTC 1 cut(s) 595
VpaK11BI GGWCC 2 cut(s) 22, 95
XapI RAATTY 3 cut(s) 55, 319, 1180
XceI RCATGY 4 cut(s) 571, 1034, 1120, 1787
XcmI CCANNNNNNNNNTGG 1 cut(s) 1610
XhoI CTCGAG 1 cut(s) 960
XmaJI CCTAGG 1 cut(s) 1640
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 5 cut(s) 228, 461, 1641, 1725, 1854
Zsp2I ATGCAT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.