pycom10g05250
ERF Family

Tetratricopeptide repeat (TPR)-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006116
Physical Location & Seq
Reverse (-)
5607527 .. 5608754
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05250.1

Sequence Viewer

Length: 948 bp
ATGTGGATCGGCTTCACCGACTTCATGGAAGCTCTGCTCGAAGAGGAGCGAGTCATAATCGACTCAATGGAGTTGGACCGAGTCAACAAGGTTCGTAAATTGCTCATGATGTCCGCCAACAAGCGTATCCCTCTGAGTAAAATCTACCATTGCAGGTCGATTTTTGGAATTCCTGAAGATTTTAGGGACAGGCCAAGATTCCGGTCCATTAGACGAGCTTGCAGTGCCAGTCTGGACGCCTTCTCAGACGAAGAGTTCTCGAAGAAGATTCAAGAGCTCGCTCTCAGATTCCAGCTCTCCGACAATTTTGACGGCGAAGATGACTTTAACGTTGGCAAAAACGCCTCGAATTCGGAACCAGAAGCGGCGAATTCTGTTGACAATCACGGCATCAGTGAGTCTGCAACGAGAATGAGAGTTGAACAGAACCAGAGCCGGTTCCAGTTGCCGCTCGCTCTAGAGCCACCGTGGCTGACCGATTGGCAGCGAAGGGACGATATTGTCGCGGAGGTTAGCATCGAGCGGAAGGCAAACAGCGTCGACGTTCCGCTCTCGCTCCGAATCATCAAGCGGAAGATGCAGTGGCAGGAGGGTTTCCGAGAAGCAGGGGAATCTGCGTGCTGCTCTGTAAAAAAGGCCTTCTCTTCCATGGTGTTCATAATCCGAGAGCTTCATAGCTACACGTTGCAGATGAAAGAGATTCTCTTCTTCGAAGATTTGCAGGGGATTTTAAACAGGATGCAGAAGGAGATGCAAGCTTCGTTCGTCTGGCTATTTCAACAGGTCTTCTCTCGGACTAAAGGCTCCGACTTTGGGAGCTGCAAAGGAAGCTGGGCGTGGCTTGGGAGTCCGAGAGGTCACTCCTCCTTCGATCATCGACCTCCTCACAGCTGTGCTCCATCTTCAAACTCTCCGAACTCCGCTGCTTCAGCTCTCCAGACTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_17 TPR_19

Protein Analysis

316

Amino Acids

36.15

Weight (kDa)

6.62

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 2 - 64 1.4e-17 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 144
AccBSI CCGCTC 3 cut(s) 451, 523, 550
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 506
AciI CCGC 8 cut(s) 114, 365, 449, 506, 523, 548, 571, 921
AclI AACGTT 1 cut(s) 330
AclWI GGATC 1 cut(s) 14
AcsI RAATTY 3 cut(s) 168, 349, 370
AcuI CTGAAG 2 cut(s) 195, 912
AcyI GRCGYC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 813
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 4 cut(s) 272, 422, 779, 906
AleI CACNNNNGTG 1 cut(s) 891
Alw21I GWGCWC 2 cut(s) 279, 898
AlwI GGATC 1 cut(s) 14
AoxI GGCC 2 cut(s) 191, 636
ApeKI GCWGC 4 cut(s) 484, 621, 819, 923
ApoI RAATTY 3 cut(s) 168, 349, 370
AspS9I GGNCC 2 cut(s) 76, 204
AsuHPI GGTGA 1 cut(s) 7
AsuII TTCGAA 1 cut(s) 711
AvaII GGWCC 2 cut(s) 76, 204
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 2 cut(s) 279, 898
BbvI GCAGC 4 cut(s) 496, 608, 806, 910
BccI CCATC 1 cut(s) 907
BceAI ACGGC 2 cut(s) 328, 403
BciVI GTATCC 1 cut(s) 137
BfaI CTAG 1 cut(s) 458
BfuAI ACCTGC 1 cut(s) 144
BfuI GTATCC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 469
BisI GCNGC 6 cut(s) 366, 449, 485, 622, 820, 924
BlsI GCNGC 6 cut(s) 367, 450, 486, 623, 821, 925
Bme18I GGWCC 2 cut(s) 76, 204
BmgT120I GGNCC 2 cut(s) 76, 204
BmiI GGNNCC 3 cut(s) 357, 440, 805
BmsI GCATC 5 cut(s) 399, 525, 567, 729, 741
BpiI GAAGAC 1 cut(s) 778
BpmI CTGGAG 1 cut(s) 920
Bpu14I TTCGAA 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 467, 648
BsaWI WCCGGW 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 813
Bse118I RCCGGY 1 cut(s) 435
Bse1I ACTGG 2 cut(s) 228, 442
Bse3DI GCAATG 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 467, 648
BseGI GGATG 1 cut(s) 744
BseLI CCNNNNNNNGG 1 cut(s) 813
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 3 cut(s) 125, 258, 298
BseNI ACTGG 2 cut(s) 228, 442
BseRI GAGGAG 3 cut(s) 59, 853, 873
BseXI GCAGC 4 cut(s) 496, 608, 806, 910
BseYI CCCAGC 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 193, 638
BsiHKAI GWGCWC 2 cut(s) 279, 898
BsiSI CCGG 2 cut(s) 202, 436
BslFI GGGAC 2 cut(s) 200, 506
BslI CCNNNNNNNGG 1 cut(s) 813
BsmFI GGGAC 2 cut(s) 200, 506
BsnI GGCC 2 cut(s) 193, 638
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 2 cut(s) 279, 898
Bsp143I GATC 2 cut(s) 6, 871
Bsp19I CCATGG 1 cut(s) 648
BspACI CCGC 8 cut(s) 114, 365, 449, 506, 523, 548, 571, 921
BspANI GGCC 2 cut(s) 193, 638
BspCNI CTCAG 3 cut(s) 126, 257, 297
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 357, 440, 805
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 1 cut(s) 14
BspT104I TTCGAA 1 cut(s) 711
BsrBI CCGCTC 3 cut(s) 451, 523, 550
BsrDI GCAATG 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 435
BsrI ACTGG 2 cut(s) 228, 442
BssAI RCCGGY 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 467, 648
BssMI GATC 2 cut(s) 6, 871
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 648
Bst4CI ACNGT 1 cut(s) 468
Bst6I CTCTTC 4 cut(s) 36, 246, 649, 710
BstACI GRCGYC 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 5 cut(s) 220, 279, 453, 619, 756
BstDEI CTNAG 3 cut(s) 134, 244, 284
BstDSI CCRYGG 2 cut(s) 467, 648
BstF5I GGATG 1 cut(s) 744
BstFNI CGCG 1 cut(s) 506
BstKTI GATC 2 cut(s) 9, 874
BstMBI GATC 2 cut(s) 6, 871
BstMWI GCNNNNNNNGC 5 cut(s) 224, 469, 577, 828, 929
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 4 cut(s) 496, 608, 806, 910
BstV2I GAAGAC 1 cut(s) 778
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 2 cut(s) 193, 638
BtgI CCRYGG 2 cut(s) 467, 648
BtsCI GGATG 1 cut(s) 744
BtsI GCAGTG 2 cut(s) 229, 587
BtsIMutI CAGTG 3 cut(s) 229, 400, 587
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 5 cut(s) 220, 279, 453, 619, 756
CciI TCATGA 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 2 cut(s) 76, 204
CseI GACGC 2 cut(s) 245, 526
CviAII CATG 3 cut(s) 25, 106, 649
DdeI CTNAG 3 cut(s) 134, 244, 284
DpnI GATC 2 cut(s) 8, 873
DpnII GATC 2 cut(s) 6, 871
DraI TTTAAA 1 cut(s) 732
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
Eam1104I CTCTTC 4 cut(s) 36, 246, 649, 710
EarI CTCTTC 4 cut(s) 36, 246, 649, 710
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 648
Eco147I AGGCCT 1 cut(s) 638
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 76, 204
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 2 cut(s) 195, 912
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 3 cut(s) 168, 349, 370
EcoT14I CCWWGG 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 648
FaeI CATG 3 cut(s) 28, 109, 652
FaiI YATR 7 cut(s) 26, 56, 107, 650, 659, 675, 946
FaqI GGGAC 2 cut(s) 200, 506
FatI CATG 3 cut(s) 24, 105, 648
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 6 cut(s) 366, 449, 485, 622, 820, 924
FokI GGATG 1 cut(s) 751
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 6 cut(s) 366, 449, 485, 622, 820, 924
FspBI CTAG 1 cut(s) 458
GluI GCNGC 6 cut(s) 366, 449, 485, 622, 820, 924
GsaI CCCAGC 1 cut(s) 835
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 2 cut(s) 193, 638
HapII CCGG 2 cut(s) 202, 436
HgaI GACGC 2 cut(s) 245, 526
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 3 cut(s) 28, 109, 652
HincII GTYRAC 3 cut(s) 85, 379, 541
HindII GTYRAC 3 cut(s) 85, 379, 541
HindIII AAGCTT 1 cut(s) 756
HpaII CCGG 2 cut(s) 202, 436
HphI GGTGA 1 cut(s) 7
Hpy166II GTNNAC 3 cut(s) 85, 379, 541
Hpy188III TCNNGA 7 cut(s) 106, 173, 233, 259, 272, 458, 937
Hpy8I GTNNAC 3 cut(s) 85, 379, 541
Hpy99I CGWCG 2 cut(s) 542, 545
HpyAV CCTTC 6 cut(s) 250, 483, 520, 649, 739, 877
HpyCH4III ACNGT 1 cut(s) 468
HpyCH4IV ACGT 3 cut(s) 330, 543, 683
HpyCH4V TGCA 9 cut(s) 153, 222, 404, 580, 688, 721, 742, 754, 822
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 469, 577, 828, 929
HpyF3I CTNAG 3 cut(s) 134, 244, 284
HpySE526I ACGT 3 cut(s) 330, 543, 683
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 3 cut(s) 28, 109, 652
Kzo9I GATC 2 cut(s) 6, 871
LmnI GCTCC 5 cut(s) 46, 561, 809, 816, 901
Lsp1109I GCAGC 4 cut(s) 496, 608, 806, 910
LweI GCATC 5 cut(s) 399, 525, 567, 729, 741
MaeI CTAG 1 cut(s) 458
MaeII ACGT 3 cut(s) 330, 543, 683
MaeIII GTNAC 1 cut(s) 857
MalI GATC 2 cut(s) 8, 873
MbiI CCGCTC 3 cut(s) 451, 523, 550
MboI GATC 2 cut(s) 6, 871
MhlI GDGCHC 2 cut(s) 279, 898
MluCI AATT 5 cut(s) 98, 168, 304, 349, 370
MlyI GAGTC 6 cut(s) 56, 60, 90, 407, 856, 934
MmeI TCCRAC 3 cut(s) 54, 324, 831
MnlI CCTC 9 cut(s) 37, 141, 355, 502, 583, 848, 874, 891, 894
MseI TTAA 2 cut(s) 327, 731
MslI CAYNNNNRTG 1 cut(s) 891
MspA1I CMGCKG 2 cut(s) 891, 923
MspI CCGG 2 cut(s) 202, 436
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 5 cut(s) 224, 469, 577, 828, 929
NcoI CCATGG 1 cut(s) 648
NdeII GATC 2 cut(s) 6, 871
NlaIII CATG 3 cut(s) 28, 109, 652
NlaIV GGNNCC 3 cut(s) 357, 440, 805
NmuCI GTSAC 1 cut(s) 857
NspV TTCGAA 1 cut(s) 711
OliI CACNNNNGTG 1 cut(s) 891
PagI TCATGA 1 cut(s) 105
PceI AGGCCT 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 6 cut(s) 198, 268, 288, 561, 611, 700
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 6 cut(s) 367, 450, 486, 623, 821, 925
PleI GAGTC 6 cut(s) 56, 59, 89, 406, 855, 934
PpsI GAGTC 6 cut(s) 56, 59, 89, 406, 855, 934
Psp124BI GAGCTC 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 831
PspN4I GGNNCC 3 cut(s) 357, 440, 805
PspPI GGNCC 2 cut(s) 76, 204
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 891
RseI CAYNNNNRTG 1 cut(s) 891
SacI GAGCTC 1 cut(s) 279
SalI GTCGAC 1 cut(s) 539
SaqAI TTAA 2 cut(s) 327, 731
SatI GCNGC 6 cut(s) 366, 449, 485, 622, 820, 924
Sau3AI GATC 2 cut(s) 6, 871
Sau96I GGNCC 2 cut(s) 76, 204
SchI GAGTC 6 cut(s) 56, 60, 90, 407, 856, 934
SduI GDGCHC 2 cut(s) 279, 898
SfaNI GCATC 5 cut(s) 399, 525, 567, 729, 741
SfuI TTCGAA 1 cut(s) 711
SgrDI CGTCGACG 1 cut(s) 539
SinI GGWCC 2 cut(s) 76, 204
SmiMI CAYNNNNRTG 1 cut(s) 891
Sse9I AATT 5 cut(s) 98, 168, 304, 349, 370
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 8 cut(s) 114, 365, 449, 506, 523, 548, 571, 921
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 279
StuI AGGCCT 1 cut(s) 638
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 1 cut(s) 468
TaiI ACGT 3 cut(s) 333, 546, 686
TaqII GACCGA 2 cut(s) 93, 491
TasI AATT 5 cut(s) 98, 168, 304, 349, 370
TauI GCSGC 2 cut(s) 368, 451
TfiI GAWTC 6 cut(s) 198, 268, 288, 561, 611, 700
Tru1I TTAA 2 cut(s) 327, 731
Tru9I TTAA 2 cut(s) 327, 731
TscAI CASTG 3 cut(s) 229, 400, 587
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 4 cut(s) 484, 621, 819, 923
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 13, 646, 662, 707
TspRI CASTG 3 cut(s) 229, 400, 587
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 76, 204
XapI RAATTY 3 cut(s) 168, 349, 370
XbaI TCTAGA 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.