Rorug05G0394300
ERF Family

Tetratricopeptide repeat (TPR)-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006116
Physical Location & Seq
Forward (+)
54107056 .. 54115341
8286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0394300.1

Sequence Viewer

Length: 5043 bp
ATGGATCCTCCGGTGGCGAAAAAGAGGGGCCGGCCGAGGAAGCGGCGGGCTGATGATCGGAAAGCGGGCCCCGAGGCGAAGAAACAGGCATTGGAGACGAGATCGATTGCGTTGTTGGGACGATACGTGTTGAAGGAGTTTGAAAGTAGGATTTATCTGGGGAAAGTTGCGTTTTATGATGTTGGATTGTATAGAGTGCACTATGAAGATGGGGATTTCGAGGACTTGGAGAGTGGTGAGATTCGGGGCATTCTTGTGGGTGAGAGTGATTTTGATGGGAGTTTGAGTGCTAAGAGGAAGAAATTGGATAAGCTGGTGGCGAAAGTGAGTGACGAAAAGAAGATTAAGGTGGATGAGAATGCGGAAAAGGAGGTGGTGGATAGGGTTGAATCCTCGGAATTGAGTGAGTGGAGCGGTAGAGTAACTATTGACAATGATGAGATTCGAGAGGATGGTGATGCTGATTTGTCTAGTGATTCGAGTGAGTGTGCAGGGGATATGGATGCCGGGGTTGATGTTGAGACCCCGGCTGTGCCGCCGCCGGAGTTGCCACCGTCTTCTGGAACCATAGGCGTGCCGGAGCCGTGTGTTTCGCATCTTTTCTCTGTATATGGTTTCATGCGGTCGTTTAGCATCTGCCTGTTCTTGAACCCGTTTACTTTGGATGATTTTGTGGGTTCGCTTAATTATCGTGCTCCAAACACATTGTTTGATGCAATTCATGTTGCTTTGTTGCGGGCCTTGAGACGTCATCTTGAAACAATCTCATCCGAAGGCTCAGAGGTAGCACAAAAATGTTTGAGGTGCATAGATTGGAGCTTGCTTGATACAGTGACTTGGCCTGTTTACTTGGTCCAGTATTTAACAATTATGGGATATGTAAAAGGACAAGAGTGGAAAGGTTTCTATGATGAGGTTTTGGACAGAGAGTATTATTTATTATCAGTTGATAGGAAGTTAAGTGTTTTGCAAATCATTTGTGATGATGTCTTAGACAGTACAGATATAAGAGCTGAATTAGACATGCGTGAAGAATCAGAAGTTGGAATAGATTATGATGCCGAAGCAACCAGTGCTCATGTAAATGGGCCAAGAAGGGTCCATCCGAGATATTCCAAAACTTCTGCATGCAAGGACAGAGAAGCAATGGAAATTGCTACTGAATCCCATGATATAAAATTATCTGGTAACTTAAATTCACTAGGTTCCAAAGTTGCTAAAGGGGAGTTTGATGCTACTGATGTTGATTCGGATTGTAACTCTGATGAATGTCGGCTTTGTGGTATGGATGGGATATTGCTTTGCTGTGATGGATGCCCATCTGCCTACCATACGAGGTGTATAGGTGTGATGAAACTGTCGATACCAGAAGGATCCTGGTATTGTCCTGAGTGCACTATCAACAAAATTGGGCCAACTATCACAGTTGGAACATCAGTTAAAGGAGCACAATTGTTTGGGATAGATACCTACGAGCACATCTTCATGGGTACTTGCAACCACTTACTTGTGCTTAAGGAAACTATTAACACACAACCATGTCTTAGATATTACAATCAGCATGACATTCCAAAAGTCCTGAAAGTTCTTTGTTCATCTGGGCAAAATACATCTTTTTATCTGGGCTTGTGCAAGGCAATTTTAGAATACTGGAATATCCCAGAAAGCATCTTATCTCTTTCAGAGCTGGCTGAATCAATGACTAATTTGGCGAAGATAAAGGAAGATGTGAACTTTTGTGGCCAGTCACTTCCTTTGTCTGGCAAGGATTGTGATAAAGTTATGGATATGGTTGAGGCTGTGAACTGTGAAACTTATGTAAATGAAGACCATTTAGGTCAAGCAGTTTCATCTTTCGATTGCCATGGTGATAGTACACCACAGGAGTACTCACATAGGAATATGGAGATTGATGAAAGGAATAAAATGGAATATGCTGTTTCGACTGGTTCTGTCACCCAGCAAGCTGATCCATCTTACTTGGTCCACAGAAGCTCAGTTGACAGATCAACTGCAATTGACCGAAGCACATGCACACCAGGGAACATCAACAGTGGTAACAAAGTGCATGCAAATGGTATGCGTCTTTCAGTGACTTCACAAAATGAAGAAGGCCATATAGGAAAGGTTTACTCCACTTCAGTTGATGATTGCAAATACAAGGGTTCTTTCTTTAAACCCCATGCATACATAAATTACTATGTGCATGGGGATTTTGCTGCATCTGCTGCTGCCAAACTGGCTGTACTTTCATCAGAAGAAGCTCGGGGTTCAGATGTCCAGGCTTCAGGCAATCCCCGGAAAGTTGCATCATCAAACAATTTATGGCAAGCAAAAGCCTTCTCACTAACAGCCTCACGTTTCTTCTGGCCAAGTTTTGAAAAGAAGCTTGTGGAGGTTCCAAGAGAGAGGTGTGGTTGGTGTCTTTCTTGTAAGGCTTCAGTTGCAAGCAAGAGAGGATGCATGCTAAATCATGCTTGTTTGAGTGCCACTAAAGGTGCTATGAAGATCCTTGCCAGCCTTCGTCCTATAAAGAGTGTGGAGGGGAATCTTGTAAGCATCGCAACATACATCTTATACATGGAGGAGAGTTTGCATGGTCTAATAACTGGCCCCTTTCTCAATGAAAATTTTAGGAAACAATTCCGCCAACAAGTTTATCAAGCATCAACCTGCAGTGCAATAAAAGTCCTATTGCTCAAACTTGAGGAAAACATCCGAACAATTGCTCTTTCCGGGGAGTGGATTAAACTGGTAGATGATTTGCTGGTTGAATCTTCTGTGATTCAAGGTCCTACGTGCACTGTTGGAACAACACAAAGACGTGCACCATACAGCAGGCGGAGCAGGAAACAGGGTGCAATTCAAGAAGTTATAGATGATGACTGCAATGACAAGAGTTTTGTATGGTGGCAAGGAGGGAAGCTGTCAAAAATCATATTCCAGAGAGCAATCTTGGCTCGCTCATTGGTTAAAAAAGCAGCTCGCCAAGGTGGATCGAGGAAAATTTTTGGTGTATCTTACGCTGATGGTCCTGATATTCCTAAAAGAAGCAGACAATCTGTATGGAGGGCTGCAGTTGAACTGAGCAAGAATGGATCCCAGCTTGCAGTTCAGGTTAGATACTTGGACTATCATTTGAGGTGGAGTGATCTTGTTCGTCCTGAGCAGAACCTCCTTGATGGGAAAGCTGCAGAGGCAGAAGCTTCTGCTTTTAGAAATGCAAGTATATGTGATAAAAAAATGTTAAAGAACAACATCGTCTATGGGGTTGCATTTGGTAGTCAAAAGCATCTGCCTAACCGTGTAATGAAAAATATCATTGAGACAGAGCAGAATCAGGATGGAACTAACAAGTTCTGGTTTCTTGAATCACGTATTCCTTTATATTTGATTAAGGAATATGAAGAAAGTGTAGGTAAAGTGTCTATTCCATCAGCTCAGGAGCCTAATCTATTGCACAAGTTACAGAGAAGGCAGCGGAAAGCAACCCGCAGGGACATATTTTTTTATCTCGAGTGTAAAAGAGACAATCTGGACTTGTCTACCTGCTCTATGTGTCAACTGGATATTTTATTGCGCAATGCAGTCAAGTGCAGTGCCTGTCAAGGTTATTGCCATGAGGGCTGTACCATAAGTTCAACGGTTTCTACAAATGAGGAAGTTGAGTTCTTGATCACTTGCAGGCGGTGCTACCATGCGAAAGCTCTTGCCCAGAAGCAAAAGTTTAAGGAGTTTCCAACTAGTCCTTTGCCCTTGCAAATGAAAGAATATCATACTCCACTGACAGTCACAACTGGTGGAAGGCCTAAAAATTATAACCAATCATTAACCTCCATCAGGGTTCAGGAGCCTCGTTCTGAGATTAAACAAGCTACTACAGACTCGGGTTTGGCAACAAAAAAACGACGTCCCATTTGTTCTTGGGGTGTTATATGGAAGAAGAAGACTCCGGAAACTGGCACCGACTTCAGGATTAATAACATACTCCTCGGGGGTCGTTCAGATGTGCATGGGTTGAAACCTGTTTGCCACTTGTGCCATATGCCATATCTCTCCGATCTGACATATATACGCTGTGAATCTTGCAAAAATTGGTACCATGCTGAAGCTGTCGAACTTGAGGAGTCAAAGATCTGTGAAGTGGCAGGCTTCAAGTGTTGCAAGTGTCGCAGGATAAAATCGCCTCTTTGTCCTTATACTGATCTTAAAGACAAGACCTTGCAAGAGAGCAAGAAGATTCGCAATAAGCGTTCAAAGCAAGAAAATCTTGGAGAGGACTCGGATTCTGCAACCAATTTTGATTCAGAGGTGTTTGAACCTGCTACTCCAGTGTTTCCCATGGAGGAAGTCTCCATTCAGGATGATGATCCTCTTCTTTTTGCTCTCTCAAGAGTGGAGCTAGTTCCAGAACACAATTCAGAAGTTAATGCTGAATGGGATATTGCTGGGCCAGGGCCACGAAAATTACCTGTGAGAAGGCATGTTAAGCGTGAAGAGGACTTGGATATATATTGTGACAGTAATAAATCTCATGCTGAAAAAACTATGCATGATGAAACAAATTACCCGTCGGAACCGATGGAGATTGCGGCATTCCCTCATGTGGAATGGGATACTTCCATGAACGGCATCAATGGTGAGATAATGGAACAGTATGAAGATCTGAACTACGATATGATGGAACCGCAAACTGTCTTTACTATCAATGAGTTGCTGGCACCTGATGACGGCGACCTTTTTGATGGAGTTGAAACATTTGCGGATTTACCAGAAAATCTGGAAAATCCATATACAACTTCACAGCATGTAGGCCCTGAGCAATATGATATGGCCACTTTCACAGACGAATCAAAGTCTGCAATTCCTGAAACATGTGCTGTTAATATGATGCAATGTCAGATATGTTTACATGCAGAACCAGCTCCTGATCTTTCTTGCCAGAACTGTGGGCTATTAATTCATAACCGTTGTTCCCCTTGGATTGAGTCATCATCTCAGAATAATAGCTGGAAGTGTGGACAGTGCCGGGAGTGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_17 TPR_19

Protein Analysis

1680

Amino Acids

188.72

Weight (kDa)

5.74

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTM_DIR17_Tudor PF21743 40 - 85 2e-14 PTM/DIR17-like, Tudor domain
DDT PF02791 196 - 251 2.7e-17 DDT domain
WHIM1 PF15612 297 - 341 1.1e-09 WSTF, HB1, Itc1p, MBD9 motif 1
PHD PF00628 424 - 465 1.9e-07 PHD-finger
Chromo_PTM PF24294 1069 - 1141 4.3e-33 Membrane-bound transcription factor PTM, chromo domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3819
AatII GACGTC 2 cut(s) 751, 3913
Acc16I TGCGCA 1 cut(s) 3581
Acc36I ACCTGC 3 cut(s) 2681, 3557, 4330
Acc65I GGTACC 1 cut(s) 4098
AccB1I GGYRCC 3 cut(s) 3962, 4098, 4720
AccBSI CCGCTC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 3545
AccIII TCCGGA 1 cut(s) 3952
AclWI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2503, 3004, 3093, 3106, 4364
AcoI YGGCCR 4 cut(s) 32, 1741, 2369, 4833
AcsI RAATTY 3 cut(s) 1195, 2629, 3006
AcuI CTGAAG 5 cut(s) 2124, 2271, 2424, 3955, 4128
AcyI GRCGYC 2 cut(s) 748, 3910
AfaI GTAC 7 cut(s) 1000, 1492, 1876, 1889, 2247, 3631, 4100
AfiI CCNNNNNNNGG 8 cut(s) 560, 1760, 2742, 3840, 3959, 3972, 4606, 4730
AflII CTTAAG 1 cut(s) 1514
AflIII ACRYGT 2 cut(s) 126, 4874
AhlI ACTAGT 1 cut(s) 3743
AjiI CACGTC 1 cut(s) 2825
AjnI CCWGG 4 cut(s) 1376, 2038, 2280, 4453
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 1026, 1058
AloI GAACNNNNNNTCC 2 cut(s) 3653, 3685
Alw21I GWGCWC 8 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
Alw26I GTCTC 6 cut(s) 89, 515, 739, 3320, 3522, 4357
Alw44I GTGCAC 4 cut(s) 197, 1393, 2800, 2826
AlwI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2503, 3004, 3093, 3106, 4364
AlwNI CAGNNNCTG 2 cut(s) 3603, 4928
Ama87I CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
Aor13HI TCCGGA 1 cut(s) 3952
ApaI GGGCCC 1 cut(s) 71
ApaLI GTGCAC 4 cut(s) 197, 1393, 2800, 2826
ApeKI GCWGC 7 cut(s) 2219, 2228, 2231, 2981, 3074, 3191, 3478
ApoI RAATTY 3 cut(s) 1195, 2629, 3006
ArsI GACNNNNNNTTYG 6 cut(s) 2885, 2917, 3900, 3932, 4556, 4588
AseI ATTAAT 2 cut(s) 3978, 4958
Asp700I GAANNNNTTC 2 cut(s) 902, 2642
Asp718I GGTACC 1 cut(s) 4098
AspLEI GCGC 1 cut(s) 3582
AsuC2I CCSGG 5 cut(s) 508, 527, 2299, 2737, 5030
AsuHPI GGTGA 6 cut(s) 248, 272, 467, 1880, 1948, 4652
AvaI CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
AvaII GGWCC 5 cut(s) 853, 1099, 1984, 2792, 3032
BaeGI GKGCMC 5 cut(s) 71, 201, 1397, 2804, 2830
BalI TGGCCA 3 cut(s) 1743, 2371, 4835
BamHI GGATCC 3 cut(s) 4, 1373, 3098
BanI GGYRCC 3 cut(s) 3962, 4098, 4720
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 3 cut(s) 549, 1833, 3953
Bbv12I GWGCWC 8 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
BbvI GCAGC 7 cut(s) 2206, 2215, 2218, 2993, 3061, 3178, 3490
BceAI ACGGC 3 cut(s) 568, 4645, 4747
BcgI CGANNNNNNTGC 2 cut(s) 2013, 2047
BciT130I CCWGG 4 cut(s) 1378, 2040, 2282, 4455
BciVI GTATCC 1 cut(s) 4609
BclI TGATCA 1 cut(s) 3675
BcnI CCSGG 5 cut(s) 508, 527, 2299, 2737, 5030
BcoDI GTCTC 6 cut(s) 89, 515, 739, 3320, 3522, 4357
BcuI ACTAGT 1 cut(s) 3743
BfaI CTAG 4 cut(s) 471, 1202, 3744, 4403
BfmI CTRYAG 4 cut(s) 2674, 3075, 3192, 3879
BfrI CTTAAG 1 cut(s) 1514
BfuAI ACCTGC 3 cut(s) 2681, 3557, 4330
BfuI GTATCC 1 cut(s) 4609
BglI GCCNNNNNGGC 2 cut(s) 2240, 3624
BglII AGATCT 2 cut(s) 4134, 4663
BmcAI AGTACT 1 cut(s) 1889
Bme1390I CCNGG 9 cut(s) 508, 527, 1378, 2040, 2282, 2299, 2737, 4455, 5030
Bme18I GGWCC 5 cut(s) 853, 1099, 1984, 2792, 3032
BmeT110I CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
BmgBI CACGTC 1 cut(s) 2825
BmrFI CCNGG 9 cut(s) 508, 527, 1378, 2040, 2282, 2299, 2737, 4455, 5030
BpiI GAAGAC 3 cut(s) 549, 1833, 3953
BplI GAGNNNNNCTC 2 cut(s) 4337, 4369
BpmI CTGGAG 1 cut(s) 4314
Bpu10I CCTNAGC 3 cut(s) 3165, 3441, 4818
BpuEI CTTGAG 4 cut(s) 763, 2726, 4142, 4375
BpuMI CCSGG 5 cut(s) 508, 527, 2299, 2737, 5030
Bsa29I ATCGAT 1 cut(s) 104
BsaAI YACGTR 3 cut(s) 127, 2799, 3377
BsaBI GATNNNNATC 2 cut(s) 1318, 4368
BsaHI GRCGYC 2 cut(s) 748, 3910
BsaI GGTCTC 1 cut(s) 515
BsaWI WCCGGW 2 cut(s) 10, 3952
BsaXI ACNNNNNCTCC 3 cut(s) 271, 301, 5024
Bsc4I CCNNNNNNNGG 8 cut(s) 560, 1760, 2742, 3840, 3959, 3972, 4606, 4730
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 4 cut(s) 1152, 2896, 3589, 4901
Bse8I GATNNNNATC 2 cut(s) 1318, 4368
BseAI TCCGGA 1 cut(s) 3952
BseBI CCWGG 4 cut(s) 1378, 2040, 2282, 4455
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 2 cut(s) 1318, 4368
BseLI CCNNNNNNNGG 8 cut(s) 560, 1760, 2742, 3840, 3959, 3972, 4606, 4730
BseMI GCAATG 4 cut(s) 1152, 2896, 3589, 4901
BseMII CTCAG 9 cut(s) 792, 1380, 2010, 3077, 3156, 3455, 3852, 4809, 5012
BseRI GAGGAG 3 cut(s) 2600, 3980, 4139
BseSI GKGCMC 5 cut(s) 71, 201, 1397, 2804, 2830
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 7 cut(s) 2206, 2215, 2218, 2993, 3061, 3178, 3490
BseYI CCCAGC 3 cut(s) 1959, 3102, 4448
BsgI GTGCAG 2 cut(s) 510, 3616
Bsh1285I CGRYCG 2 cut(s) 35, 626
BshNI GGYRCC 3 cut(s) 3962, 4098, 4720
BshVI ATCGAT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 35, 626
BsiHKAI GWGCWC 8 cut(s) 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
BsiHKCI CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
BslFI GGGAC 3 cut(s) 132, 3512, 3897
BslI CCNNNNNNNGG 8 cut(s) 560, 1760, 2742, 3840, 3959, 3972, 4606, 4730
BsmAI GTCTC 6 cut(s) 89, 515, 739, 3320, 3522, 4357
BsmBI CGTCTC 2 cut(s) 89, 739
BsmFI GGGAC 3 cut(s) 132, 3512, 3897
BsmI GAATGC 3 cut(s) 249, 364, 4595
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 9 cut(s) 71, 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
Bsp13I TCCGGA 1 cut(s) 3952
Bsp19I CCATGG 2 cut(s) 1864, 4341
BspCNI CTCAG 9 cut(s) 791, 1381, 2009, 3078, 3157, 3454, 3853, 4810, 5011
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 3952
BspMAI CTGCAG 3 cut(s) 2678, 3079, 3196
BspMI ACCTGC 3 cut(s) 2681, 3557, 4330
BspPI GGATC 9 cut(s) 12, 1368, 1381, 1964, 2503, 3004, 3093, 3106, 4364
BspT107I GGYRCC 3 cut(s) 3962, 4098, 4720
BspTI CTTAAG 1 cut(s) 1514
BspTNI GGTCTC 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 4 cut(s) 1152, 2896, 3589, 4901
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssNI GRCGYC 2 cut(s) 748, 3910
BssT1I CCWWGG 4 cut(s) 1864, 2989, 4341, 4979
Bst2UI CCWGG 4 cut(s) 1378, 2040, 2282, 4455
Bst6I CTCTTC 2 cut(s) 4380, 4491
BstACI GRCGYC 2 cut(s) 748, 3910
BstAFI CTTAAG 1 cut(s) 1514
BstAPI GCANNNNNTGC 3 cut(s) 1073, 2228, 3690
BstBAI YACGTR 3 cut(s) 127, 2799, 3377
BstDSI CCRYGG 2 cut(s) 1864, 4341
BstENI CCTNNNNNAGG 1 cut(s) 3838
BstHHI GCGC 1 cut(s) 3582
BstMAI GTCTC 6 cut(s) 89, 515, 739, 3320, 3522, 4357
BstMCI CGRYCG 2 cut(s) 35, 626
BstNI CCWGG 4 cut(s) 1378, 2040, 2282, 4455
BstNSI RCATGY 9 cut(s) 1027, 1131, 2034, 2072, 2467, 4487, 4811, 4878, 4916
BstSCI CCNGG 9 cut(s) 506, 525, 1376, 2038, 2280, 2297, 2735, 4453, 5028
BstSFI CTRYAG 4 cut(s) 2674, 3075, 3192, 3879
BstSLI GKGCMC 5 cut(s) 71, 201, 1397, 2804, 2830
BstV1I GCAGC 7 cut(s) 2206, 2215, 2218, 2993, 3061, 3178, 3490
BstV2I GAAGAC 3 cut(s) 549, 1833, 3953
BstX2I RGATCY 6 cut(s) 4, 1373, 2508, 3098, 4134, 4663
BstXI CCANNNNNNTGG 1 cut(s) 4949
BstYI RGATCY 6 cut(s) 4, 1373, 2508, 3098, 4134, 4663
BstZI CGGCCG 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 104
BsuI GTATCC 1 cut(s) 4609
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 2 cut(s) 1864, 4341
BtgZI GCGATG 1 cut(s) 2545
BtrI CACGTC 1 cut(s) 2825
BtsI GCAGTG 2 cut(s) 2683, 3604
BveI ACCTGC 3 cut(s) 2681, 3557, 4330
CaiI CAGNNNCTG 2 cut(s) 3603, 4928
CfoI GCGC 1 cut(s) 3582
Cfr10I RCCGGY 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 104
CseI GACGC 1 cut(s) 2072
Csp6I GTAC 7 cut(s) 999, 1491, 1875, 1888, 2246, 3630, 4099
CspCI CAANNNNNGTGG 2 cut(s) 2125, 2160
CviQI GTAC 7 cut(s) 999, 1491, 1875, 1888, 2246, 3630, 4099
DraI TTTAAA 1 cut(s) 2176
EaeI YGGCCR 4 cut(s) 32, 1741, 2369, 4833
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 4380, 4491
EarI CTCTTC 2 cut(s) 4380, 4491
EciI GGCGGA 2 cut(s) 2636, 2857
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 4 cut(s) 1864, 2989, 4341, 4979
Eco147I AGGCCT 1 cut(s) 3808
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 5 cut(s) 853, 1099, 1984, 2792, 3032
Eco52I CGGCCG 1 cut(s) 32
Eco57I CTGAAG 5 cut(s) 2124, 2271, 2424, 3955, 4128
Eco88I CYCGRG 5 cut(s) 71, 2265, 3515, 3886, 3992
EcoNI CCTNNNNNAGG 1 cut(s) 3838
EcoO109I RGGNCCY 3 cut(s) 68, 2792, 4814
EcoRII CCWGG 4 cut(s) 1376, 2038, 2280, 4453
EcoT14I CCWWGG 4 cut(s) 1864, 2989, 4341, 4979
EcoT22I ATGCAT 3 cut(s) 2188, 2465, 4554
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 4 cut(s) 1864, 2989, 4341, 4979
Esp3I CGTCTC 2 cut(s) 89, 739
FalI AAGNNNNNCTT 2 cut(s) 4254, 4286
FaqI GGGAC 3 cut(s) 132, 3512, 3897
FauI CCCGC 4 cut(s) 39, 58, 729, 3500
FauNDI CATATG 1 cut(s) 4044
FbaI TGATCA 1 cut(s) 3675
FblI GTMKAC 1 cut(s) 3545
FriOI GRGCYC 1 cut(s) 71
FseI GGCCGGCC 1 cut(s) 34
FspBI CTAG 4 cut(s) 471, 1202, 3744, 4403
FspI TGCGCA 1 cut(s) 3581
GlaI GCGC 1 cut(s) 3581
GsaI CCCAGC 3 cut(s) 1963, 3106, 4452
GsuI CTGGAG 1 cut(s) 4314
HgaI GACGC 1 cut(s) 2072
HhaI GCGC 1 cut(s) 3582
Hin1I GRCGYC 2 cut(s) 748, 3910
Hin6I GCGC 1 cut(s) 3580
HinP1I GCGC 1 cut(s) 3580
HincII GTYRAC 2 cut(s) 2002, 3563
HindII GTYRAC 2 cut(s) 2002, 3563
HindIII AAGCTT 2 cut(s) 2387, 3204
HphI GGTGA 6 cut(s) 248, 272, 467, 1880, 1948, 4652
Hpy99I CGWCG 2 cut(s) 3912, 4576
HpyCH4IV ACGT 7 cut(s) 126, 748, 2359, 2798, 2824, 3376, 3910
HpySE526I ACGT 7 cut(s) 126, 748, 2359, 2798, 2824, 3376, 3910
Hsp92I GRCGYC 2 cut(s) 748, 3910
HspAI GCGC 1 cut(s) 3580
Kpn2I TCCGGA 1 cut(s) 3952
KpnI GGTACC 1 cut(s) 4102
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 3675
Lsp1109I GCAGC 7 cut(s) 2206, 2215, 2218, 2993, 3061, 3178, 3490
MaeI CTAG 4 cut(s) 471, 1202, 3744, 4403
MaeII ACGT 7 cut(s) 126, 748, 2359, 2798, 2824, 3376, 3910
MbiI CCGCTC 1 cut(s) 414
MfeI CAATTG 3 cut(s) 1451, 2016, 2724
MflI RGATCY 6 cut(s) 4, 1373, 2508, 3098, 4134, 4663
MhlI GDGCHC 9 cut(s) 71, 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
MlsI TGGCCA 3 cut(s) 1743, 2371, 4835
MluNI TGGCCA 3 cut(s) 1743, 2371, 4835
MlyI GAGTC 5 cut(s) 3878, 3943, 4136, 4274, 4997
MmeI TCCRAC 6 cut(s) 163, 1024, 1408, 2788, 3764, 4554
Mox20I TGGCCA 3 cut(s) 1743, 2371, 4835
Mph1103I ATGCAT 3 cut(s) 2188, 2465, 4554
MroI TCCGGA 1 cut(s) 3952
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 2 cut(s) 902, 2642
MscI TGGCCA 3 cut(s) 1743, 2371, 4835
MslI CAYNNNNRTG 7 cut(s) 254, 572, 793, 1083, 1484, 1537, 2073
Msp20I TGGCCA 3 cut(s) 1743, 2371, 4835
MspA1I CMGCKG 1 cut(s) 3481
MspCI CTTAAG 1 cut(s) 1514
MspR9I CCNGG 9 cut(s) 508, 527, 1378, 2040, 2282, 2299, 2737, 4455, 5030
MunI CAATTG 3 cut(s) 1451, 2016, 2724
Mva1269I GAATGC 3 cut(s) 249, 364, 4595
MvaI CCWGG 4 cut(s) 1378, 2040, 2282, 4455
NaeI GCCGGC 1 cut(s) 32
NciI CCSGG 5 cut(s) 508, 527, 2299, 2737, 5030
NcoI CCATGG 2 cut(s) 1864, 4341
NdeI CATATG 1 cut(s) 4044
NgoMIV GCCGGC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3790, 4517
NsbI TGCGCA 1 cut(s) 3581
NsiI ATGCAT 3 cut(s) 2188, 2465, 4554
NspI RCATGY 9 cut(s) 1027, 1131, 2034, 2072, 2467, 4487, 4811, 4878, 4916
PaeI GCATGC 3 cut(s) 1131, 2072, 2467
PaeR7I CTCGAG 1 cut(s) 3515
PceI AGGCCT 1 cut(s) 3808
PciI ACATGT 1 cut(s) 4874
PcsI WCGNNNNNNNCGW 1 cut(s) 4249
PctI GAATGC 3 cut(s) 249, 364, 4595
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 2 cut(s) 902, 2642
PleI GAGTC 5 cut(s) 3878, 3943, 4135, 4274, 4996
PpsI GAGTC 5 cut(s) 3878, 3943, 4135, 4274, 4996
Ppu21I YACGTR 3 cut(s) 127, 2799, 3377
PpuMI RGGWCCY 1 cut(s) 2792
PscI ACATGT 1 cut(s) 4874
PshBI ATTAAT 2 cut(s) 3978, 4958
PsiI TTATAA 1 cut(s) 3819
Psp5II RGGWCCY 1 cut(s) 2792
Psp6I CCWGG 4 cut(s) 1376, 2038, 2280, 4453
PspFI CCCAGC 3 cut(s) 1959, 3102, 4448
PspGI CCWGG 4 cut(s) 1376, 2038, 2280, 4453
PspOMI GGGCCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 2792
PsrI GAACNNNNNNTAC 2 cut(s) 3622, 3654
PstI CTGCAG 3 cut(s) 2678, 3079, 3196
PstNI CAGNNNCTG 2 cut(s) 3603, 4928
PsuI RGATCY 6 cut(s) 4, 1373, 2508, 3098, 4134, 4663
RigI GGCCGGCC 1 cut(s) 34
RsaI GTAC 7 cut(s) 1000, 1492, 1876, 1889, 2247, 3631, 4100
RsaNI GTAC 7 cut(s) 999, 1491, 1875, 1888, 2246, 3630, 4099
RseI CAYNNNNRTG 7 cut(s) 254, 572, 793, 1083, 1484, 1537, 2073
ScaI AGTACT 1 cut(s) 1889
SchI GAGTC 5 cut(s) 3878, 3943, 4136, 4274, 4997
ScrFI CCNGG 9 cut(s) 508, 527, 1378, 2040, 2282, 2299, 2737, 4455, 5030
SduI GDGCHC 9 cut(s) 71, 201, 697, 1078, 1397, 1450, 1479, 2804, 2830
SfcI CTRYAG 4 cut(s) 2674, 3075, 3192, 3879
Sfr274I CTCGAG 1 cut(s) 3515
SinI GGWCC 5 cut(s) 853, 1099, 1984, 2792, 3032
SlaI CTCGAG 1 cut(s) 3515
SmiMI CAYNNNNRTG 7 cut(s) 254, 572, 793, 1083, 1484, 1537, 2073
SmlI CTYRAG 6 cut(s) 742, 1514, 2705, 3515, 4121, 4390
SmoI CTYRAG 6 cut(s) 742, 1514, 2705, 3515, 4121, 4390
SpeI ACTAGT 1 cut(s) 3743
SphI GCATGC 3 cut(s) 1131, 2072, 2467
SseBI AGGCCT 1 cut(s) 3808
SspMI CTAG 4 cut(s) 471, 1202, 3744, 4403
StuI AGGCCT 1 cut(s) 3808
StyD4I CCNGG 9 cut(s) 506, 525, 1376, 2038, 2280, 2297, 2735, 4453, 5028
StyI CCWWGG 4 cut(s) 1864, 2989, 4341, 4979
TaiI ACGT 7 cut(s) 129, 751, 2362, 2801, 2827, 3379, 3913
TaqII GACCGA 1 cut(s) 2037
TatI WGTACW 4 cut(s) 998, 1874, 1887, 2245
TauI GCSGC 4 cut(s) 46, 538, 541, 4595
TseFI GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3790, 4517
TseI GCWGC 7 cut(s) 2219, 2228, 2231, 2981, 3074, 3191, 3478
Tsp45I GTSAC 7 cut(s) 329, 832, 1746, 1954, 2092, 3790, 4517
Vha464I CTTAAG 1 cut(s) 1514
VneI GTGCAC 4 cut(s) 197, 1393, 2800, 2826
VpaK11BI GGWCC 5 cut(s) 853, 1099, 1984, 2792, 3032
VspI ATTAAT 2 cut(s) 3978, 4958
XagI CCTNNNNNAGG 1 cut(s) 3838
XapI RAATTY 3 cut(s) 1195, 2629, 3006
XceI RCATGY 9 cut(s) 1027, 1131, 2034, 2072, 2467, 4487, 4811, 4878, 4916
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XhoI CTCGAG 1 cut(s) 3515
XmiI GTMKAC 1 cut(s) 3545
XmnI GAANNNNTTC 2 cut(s) 902, 2642
XspI CTAG 4 cut(s) 471, 1202, 3744, 4403
ZraI GACGTC 2 cut(s) 749, 3911
ZrmI AGTACT 1 cut(s) 1889
Zsp2I ATGCAT 3 cut(s) 2188, 2465, 4554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.