pycom10g06430

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser Thr consensus motif in nascent polypeptide chains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006719
Physical Location & Seq
Forward (+)
7455030 .. 7460326
5297 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06430.2

Sequence Viewer

Length: 1314 bp
ATGTCGAAGCTCCTCGGCGTCCTTGCCTTCCTAACCCTAACCCTCCCATTTCTAGCCCACTCCTTCTCGCCGGATCACCCCACCGACCGCCGCGTCCTCGTCCTACTCGACGACCTCGCCGTCAAATCCTCCCACTCCTTGTTCTTCAAATCCCTCCAGTCTCGAGGCTTCGAGCTCGATTTCAAGCTCGCCGACGATCCCAAGATCTCCTTGCAGCGATACGGCCTCTACAGCTACGACGCCTTGATCCTCTTTTCTCCTTCCGTCGATCGTTTTGGAGGATCCATTGACCTGGCTGCTATACTGGATTTCGTGGATAGCGGTCATGATTTGATCATTGCAGCTGATACCAATGCATCTGATTTGATCCGGCAGATCGCGGTTGAGTGCGGAGTTGATTTCGATGAGGATCCTGGTGCTATGGTCATAGATCACACAAGCTATGCAGTTTCAGACACTGAAGGAGATCATACAGTGATTGCTAGTGATGATTTCATCAAGTCTGACGTAATTTTGGGTAGCGAGAAAATTGAGGCTCCTGTACTTTTCCAAGGGATTGGCCATGCAGTAAACTCTGAAAATAGCCTGGTTTTGAAAGTTCTCTCAGCTTCACCTTCAGCATATTCTGCTAACCCCAAAAGCAAGCTGTCAAATCCTCCATCGCTCACTGGATCTGCTATATCTTTAGTTTCAATTGTGCAGGCAAGAAACAATGCTCGGGTTTTGATCTCTGGTTCGTTGAGTTTGTTCAGCGACAGGTTTTTCAGATCCAAGGTGCAGAAGGCTGGGAGTTCAAAGAAACATGAGAAATCGTCAAATGAGCTGTTTCTGACTGAACTTAGCAAATGGGTCTTCCATGAAAGAGGTCATCTCAAGGCTGTTAATGTAAAACACAACAGAGTTGGGGAAGCAGATGAACCTGCAATGTACAGGATCAACGATGACCTGCAATATTCGGTCGAGATTTATGAATGGCATGGACAACGATGGGAACCATATGTTGCTGATGATGTCCAAGTGCAGTTTTACATGATGAGTCCCTATGTTCTGAAAACCTTGTCGACTGATCAGAAGGGTCGTTACTTTACAGATTTTAAGGTTCCAGATGTTTATGGGGTTTTCCAATTCAAGGTTGAGTACCAGAGGCTTGGCTACACAAGTTTGTCTCTCTCAAAGCAGATCGATCCTGTTCGACCATTTAGACACAATGAATACGAAAGATTTATACCAGCAGCTTACCCCTATTATGGAGCAGCATTTTCAATGATGACAGGTTTCTTCGTCTTCACAGTCGTCCACCTGTACAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 ko04141 map00510 map00513 map01100 map04141
Pfam Domains
Protein Families
DDOST_48kD

Protein Analysis

438

Amino Acids

49.02

Weight (kDa)

5.78

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 92, 119
Acc36I ACCTGC 2 cut(s) 928, 954
AccI GTMKAC 1 cut(s) 1061
AccII CGCG 2 cut(s) 93, 380
AciI CCGC 5 cut(s) 88, 91, 321, 380, 390
AcoI YGGCCR 1 cut(s) 559
AcuI CTGAAG 2 cut(s) 480, 600
AcyI GRCGYC 2 cut(s) 18, 240
AfaI GTAC 4 cut(s) 543, 929, 1139, 1304
AfiI CCNNNNNNNGG 2 cut(s) 1129, 1247
AgsI TTSAA 7 cut(s) 148, 184, 595, 693, 795, 1129, 1263
AjnI CCWGG 3 cut(s) 291, 412, 585
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 165, 1170
AlwNI CAGNNNCTG 1 cut(s) 458
Ama87I CYCGRG 2 cut(s) 162, 717
AoxI GGCC 2 cut(s) 223, 559
ApeKI GCWGC 5 cut(s) 214, 296, 341, 1232, 1253
AsuHPI GGTGA 2 cut(s) 68, 603
AvaI CYCGRG 2 cut(s) 162, 717
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 2 cut(s) 281, 409
BanII GRGCYC 1 cut(s) 177
BarI GAAGNNNNNNTAC 2 cut(s) 1064, 1096
BbsI GAAGAC 2 cut(s) 844, 1276
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 5 cut(s) 226, 283, 353, 1244, 1265
BccI CCATC 2 cut(s) 667, 981
BceAI ACGGC 2 cut(s) 104, 238
BciT130I CCWGG 3 cut(s) 293, 414, 587
BclI TGATCA 2 cut(s) 333, 1066
BcoDI GTCTC 2 cut(s) 165, 1170
BfaI CTAG 2 cut(s) 53, 483
BfmI CTRYAG 1 cut(s) 229
BfuAI ACCTGC 2 cut(s) 928, 954
BglII AGATCT 1 cut(s) 204
BisI GCNGC 6 cut(s) 91, 215, 297, 342, 1233, 1254
BlsI GCNGC 6 cut(s) 92, 216, 298, 343, 1234, 1255
Bme1390I CCNGG 3 cut(s) 293, 414, 587
BmeT110I CYCGRG 2 cut(s) 162, 717
BmiI GGNNCC 5 cut(s) 283, 411, 537, 993, 1101
BmrFI CCNGG 3 cut(s) 293, 414, 587
BmsI GCATC 1 cut(s) 365
BpiI GAAGAC 2 cut(s) 844, 1276
BplI GAGNNNNNCTC 2 cut(s) 855, 887
BpmI CTGGAG 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 857
Bsa29I ATCGAT 1 cut(s) 1182
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 18, 240
BsaJI CCNNGG 3 cut(s) 13, 550, 771
Bsc4I CCNNNNNNNGG 2 cut(s) 1129, 1247
Bse1I ACTGG 3 cut(s) 157, 309, 673
Bse3DI GCAATG 2 cut(s) 336, 930
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 3 cut(s) 293, 414, 587
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 3 cut(s) 13, 550, 771
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 1129, 1247
BseMI GCAATG 2 cut(s) 336, 930
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 3 cut(s) 157, 309, 673
BseXI GCAGC 5 cut(s) 226, 283, 353, 1244, 1265
BseYI CCCAGC 1 cut(s) 785
BsgI GTGCAG 3 cut(s) 719, 797, 1040
Bsh1236I CGCG 2 cut(s) 93, 380
Bsh1285I CGRYCG 3 cut(s) 88, 271, 960
BshFI GGCC 2 cut(s) 225, 561
BshVI ATCGAT 1 cut(s) 1182
BsiEI CGRYCG 3 cut(s) 88, 271, 960
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 2 cut(s) 162, 717
BsiSI CCGG 2 cut(s) 71, 370
BslFI GGGAC 1 cut(s) 1023
BslI CCNNNNNNNGG 2 cut(s) 1129, 1247
BsmAI GTCTC 2 cut(s) 165, 1170
BsmFI GGGAC 1 cut(s) 1023
BsnI GGCC 2 cut(s) 225, 561
BsoBI CYCGRG 2 cut(s) 162, 717
Bsp1286I GDGCHC 1 cut(s) 177
Bsp1407I TGTACA 2 cut(s) 927, 1302
BspACI CCGC 5 cut(s) 88, 91, 321, 380, 390
BspANI GGCC 2 cut(s) 225, 561
BspCNI CTCAG 1 cut(s) 617
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 2 cut(s) 93, 380
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 5 cut(s) 283, 411, 537, 993, 1101
BspMI ACCTGC 2 cut(s) 928, 954
BsrDI GCAATG 2 cut(s) 336, 930
BsrGI TGTACA 2 cut(s) 927, 1302
BsrI ACTGG 3 cut(s) 157, 309, 673
BssECI CCNNGG 3 cut(s) 13, 550, 771
BssNI GRCGYC 2 cut(s) 18, 240
BssT1I CCWWGG 2 cut(s) 550, 771
Bst2UI CCWGG 3 cut(s) 293, 414, 587
Bst4CI ACNGT 2 cut(s) 475, 1291
BstACI GRCGYC 2 cut(s) 18, 240
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 2 cut(s) 927, 1302
BstC8I GCNNGC 3 cut(s) 189, 644, 702
BstDEI CTNAG 2 cut(s) 604, 839
BstFNI CGCG 2 cut(s) 93, 380
BstMAI GTCTC 2 cut(s) 165, 1170
BstMCI CGRYCG 3 cut(s) 88, 271, 960
BstMWI GCNNNNNNNGC 2 cut(s) 231, 626
BstNI CCWGG 3 cut(s) 293, 414, 587
BstSCI CCNGG 3 cut(s) 291, 412, 585
BstSFI CTRYAG 1 cut(s) 229
BstUI CGCG 2 cut(s) 93, 380
BstV1I GCAGC 5 cut(s) 226, 283, 353, 1244, 1265
BstV2I GAAGAC 2 cut(s) 844, 1276
BstX2I RGATCY 5 cut(s) 204, 281, 409, 671, 767
BstXI CCANNNNNNTGG 3 cut(s) 292, 557, 1148
BstYI RGATCY 5 cut(s) 204, 281, 409, 671, 767
Bsu15I ATCGAT 1 cut(s) 1182
BsuRI GGCC 2 cut(s) 225, 561
BsuTUI ATCGAT 1 cut(s) 1182
BtgZI GCGATG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 456, 480, 666
BveI ACCTGC 2 cut(s) 928, 954
Cac8I GCNNGC 3 cut(s) 189, 644, 702
CaiI CAGNNNCTG 1 cut(s) 458
CciI TCATGA 1 cut(s) 325
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 3 cut(s) 7, 82, 248
Csp6I GTAC 4 cut(s) 542, 928, 1138, 1303
CviAII CATG 6 cut(s) 326, 563, 803, 857, 977, 1030
CviQI GTAC 4 cut(s) 542, 928, 1138, 1303
DdeI CTNAG 2 cut(s) 604, 839
DrdI GACNNNNNNGTC 2 cut(s) 92, 119
DseDI GACNNNNNNGTC 2 cut(s) 92, 119
EaeI YGGCCR 1 cut(s) 559
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 550, 771
Eco24I GRGCYC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 480, 600
Eco88I CYCGRG 2 cut(s) 162, 717
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 291, 412, 585
EcoT14I CCWWGG 2 cut(s) 550, 771
EcoT22I ATGCAT 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 550, 771
FaeI CATG 6 cut(s) 329, 566, 806, 860, 980, 1033
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 1023
FatI CATG 6 cut(s) 325, 562, 802, 856, 976, 1029
FauNDI CATATG 1 cut(s) 997
FbaI TGATCA 2 cut(s) 333, 1066
FblI GTMKAC 1 cut(s) 1061
Fnu4HI GCNGC 6 cut(s) 91, 215, 297, 342, 1233, 1254
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 6 cut(s) 91, 215, 297, 342, 1233, 1254
FspBI CTAG 2 cut(s) 53, 483
GluI GCNGC 6 cut(s) 91, 215, 297, 342, 1233, 1254
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 2 cut(s) 225, 561
HapII CCGG 2 cut(s) 71, 370
HgaI GACGC 3 cut(s) 7, 82, 248
Hin1I GRCGYC 2 cut(s) 18, 240
Hin1II CATG 6 cut(s) 329, 566, 806, 860, 980, 1033
HincII GTYRAC 1 cut(s) 1062
HindII GTYRAC 1 cut(s) 1062
HinfI GANTC 1 cut(s) 1036
HpaII CCGG 2 cut(s) 71, 370
HphI GGTGA 2 cut(s) 68, 603
Hpy166II GTNNAC 3 cut(s) 571, 1062, 1297
Hpy188I TCNGA 8 cut(s) 361, 454, 505, 577, 767, 831, 1050, 1071
Hpy188III TCNNGA 4 cut(s) 162, 326, 961, 1103
Hpy8I GTNNAC 3 cut(s) 571, 1062, 1297
Hpy99I CGWCG 4 cut(s) 113, 197, 242, 269
HpyAV CCTTC 7 cut(s) 37, 73, 270, 455, 624, 775, 1066
HpyCH4III ACNGT 2 cut(s) 475, 1291
HpyCH4IV ACGT 1 cut(s) 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 626
HpyF3I CTNAG 2 cut(s) 604, 839
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 2 cut(s) 18, 240
Hsp92II CATG 6 cut(s) 329, 566, 806, 860, 980, 1033
Ksp22I TGATCA 2 cut(s) 333, 1066
LmnI GCTCC 3 cut(s) 15, 541, 1250
Lsp1109I GCAGC 5 cut(s) 226, 283, 353, 1244, 1265
LweI GCATC 1 cut(s) 365
MaeI CTAG 2 cut(s) 53, 483
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 1 cut(s) 1079
MboII GAAGA 4 cut(s) 136, 844, 1270, 1276
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 5 cut(s) 204, 281, 409, 671, 767
MhlI GDGCHC 1 cut(s) 177
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 4 cut(s) 510, 528, 693, 1124
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 1 cut(s) 1045
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 1 cut(s) 358
MscI TGGCCA 1 cut(s) 561
MseI TTAA 2 cut(s) 882, 1095
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 2 cut(s) 71, 370
MspR9I CCNGG 3 cut(s) 293, 414, 587
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 3 cut(s) 293, 414, 587
MvnI CGCG 2 cut(s) 93, 380
MwoI GCNNNNNNNGC 2 cut(s) 231, 626
NdeI CATATG 1 cut(s) 997
NlaIII CATG 6 cut(s) 329, 566, 806, 860, 980, 1033
NlaIV GGNNCC 5 cut(s) 283, 411, 537, 993, 1101
NsiI ATGCAT 1 cut(s) 358
PaeR7I CTCGAG 1 cut(s) 162
PagI TCATGA 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 117
PkrI GCNGC 6 cut(s) 92, 216, 298, 343, 1234, 1255
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 1 cut(s) 1044
PpsI GAGTC 1 cut(s) 1044
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 291, 412, 585
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 3 cut(s) 291, 412, 585
PspN4I GGNNCC 5 cut(s) 283, 411, 537, 993, 1101
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 5 cut(s) 204, 281, 409, 671, 767
PvuI CGATCG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 4 cut(s) 543, 929, 1139, 1304
RsaNI GTAC 4 cut(s) 542, 928, 1138, 1303
SacI GAGCTC 1 cut(s) 177
SalI GTCGAC 1 cut(s) 1060
SaqAI TTAA 2 cut(s) 882, 1095
SatI GCNGC 6 cut(s) 91, 215, 297, 342, 1233, 1254
SchI GAGTC 1 cut(s) 1045
ScrFI CCNGG 3 cut(s) 293, 414, 587
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 162
SmlI CTYRAG 2 cut(s) 162, 872
SmoI CTYRAG 2 cut(s) 162, 872
Sse9I AATT 4 cut(s) 510, 528, 693, 1124
SsiI CCGC 5 cut(s) 88, 91, 321, 380, 390
SspI AATATT 1 cut(s) 953
SspMI CTAG 2 cut(s) 53, 483
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 3 cut(s) 291, 412, 585
StyI CCWWGG 2 cut(s) 550, 771
TaaI ACNGT 2 cut(s) 475, 1291
TaiI ACGT 1 cut(s) 510
TaqII GACCGA 1 cut(s) 946
TasI AATT 4 cut(s) 510, 528, 693, 1124
TatI WGTACW 3 cut(s) 541, 927, 1302
TauI GCSGC 1 cut(s) 93
Tru1I TTAA 2 cut(s) 882, 1095
Tru9I TTAA 2 cut(s) 882, 1095
TscAI CASTG 3 cut(s) 463, 480, 673
TseI GCWGC 5 cut(s) 214, 296, 341, 1232, 1253
TspDTI ATGAA 5 cut(s) 484, 873, 930, 984, 1224
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 3 cut(s) 463, 480, 673
XhoI CTCGAG 1 cut(s) 162
XmiI GTMKAC 1 cut(s) 1061
XspI CTAG 2 cut(s) 53, 483
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.