pycom10g09990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007855
Physical Location & Seq
Reverse (-)
13230506 .. 13231806
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGGCTCTCAAACCGCTGTTTCTGCTCCTCCTAACGGCACAATTCCTACCGTCTTCTCTCTCTCTGATTTCCGCGCCGGAGCTCCGCCCTCCCTACGCCACCTCCAGAGCCATCTCCGATTTGAAGGAGGCGATCGTGAAGGGATTAGGGTTTCAGGCGGAGGATCTGAAGGTGTCGGGGTTCGATTTGAGGGACGCGCAGGTGGGGCATTCGGTGGCGTACGAGTTCGACGTGGAGGTCGACAACAAGGTCCTACCTTTCAAGCTTCTGGAGGACGTCGACCGCTGGGATTACGTCGATTTGCCCATTTTTCGGGTCGAGGACGAAAATGGGTTGGTCCAGAAAGGCAAATCGGATGGTGGGTTGCCTCCGGTTTTGGCTCCATTTCAGCTGGCTGGGCCCATGGAGCTCTGGATTCAGGACGCCAAAGACATGAGGATTTCGCTCCCTCATGATGTGGATGCTGGTGTGTTGAAGAAGGTGATGTTAGCAGAAGGAGCTGTGGTCACAGTGAAGGGTGCGAGGTCTGTGAGCCTACGCCACCCTCTTCAGCTCCCATTGCCATTGAACCGAACCAGCAATGGGTTTGCTTCTGGTCTTGTGCTGAATGGCTGCGCCATGCTTCTCGAACCCAGTCATCTCCGTTGTTGTCTCTCCGCATTGTTGGTCCGACTTCCCTCACATCACCGTCATCAACCTCTCCCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

25.85

Weight (kDa)

6.16

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 2 cut(s) 236, 248
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 190
AccI GTMKAC 2 cut(s) 240, 279
AccII CGCG 2 cut(s) 74, 197
AciI CCGC 6 cut(s) 14, 72, 85, 158, 283, 657
AclWI GGATC 1 cut(s) 171
AcuI CTGAAG 2 cut(s) 188, 533
AcyI GRCGYC 2 cut(s) 276, 423
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 34, 312, 582
AgsI TTSAA 4 cut(s) 124, 262, 475, 568
AjiI CACGTC 1 cut(s) 232
AluBI AGCT 6 cut(s) 82, 265, 391, 409, 500, 553
AluI AGCT 6 cut(s) 82, 265, 391, 409, 500, 553
Alw21I GWGCWC 2 cut(s) 84, 411
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 1 cut(s) 171
AoxI GGCC 1 cut(s) 398
ApaI GGGCCC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 612
AspLEI GCGC 3 cut(s) 76, 199, 617
AspS9I GGNCC 5 cut(s) 250, 337, 398, 399, 667
AsuHPI GGTGA 2 cut(s) 493, 677
AvaII GGWCC 3 cut(s) 250, 337, 667
BaeGI GKGCMC 1 cut(s) 402
BanII GRGCYC 3 cut(s) 84, 402, 411
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 2 cut(s) 84, 411
BbvI GCAGC 1 cut(s) 599
BccI CCATC 2 cut(s) 119, 350
BceAI ACGGC 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 1 cut(s) 613
BlsI GCNGC 1 cut(s) 614
Bme18I GGWCC 3 cut(s) 250, 337, 667
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 5 cut(s) 250, 337, 398, 399, 667
BmiI GGNNCC 2 cut(s) 381, 400
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 1 cut(s) 451
BmuI ACTGGG 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 2 cut(s) 88, 290
BsaHI GRCGYC 2 cut(s) 276, 423
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 312, 582
Bse1I ACTGG 1 cut(s) 633
Bse3DI GCAATG 2 cut(s) 557, 586
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 2 cut(s) 361, 466
BseLI CCNNNNNNNGG 3 cut(s) 34, 312, 582
BseMI GCAATG 2 cut(s) 557, 586
BseNI ACTGG 1 cut(s) 633
BseRI GAGGAG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 1 cut(s) 599
BseYI CCCAGC 2 cut(s) 285, 395
Bsh1236I CGCG 2 cut(s) 74, 197
Bsh1285I CGRYCG 2 cut(s) 135, 283
BshFI GGCC 1 cut(s) 400
BsiEI CGRYCG 2 cut(s) 135, 283
BsiHKAI GWGCWC 2 cut(s) 84, 411
BsiSI CCGG 2 cut(s) 77, 371
BsiWI CGTACG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 3 cut(s) 34, 312, 582
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 400
Bsp120I GGGCCC 1 cut(s) 398
Bsp1286I GDGCHC 3 cut(s) 84, 402, 411
Bsp143I GATC 2 cut(s) 132, 163
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 6 cut(s) 14, 72, 85, 158, 283, 657
BspANI GGCC 1 cut(s) 400
BspFNI CGCG 2 cut(s) 74, 197
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 2 cut(s) 381, 400
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 1 cut(s) 171
BsrDI GCAATG 2 cut(s) 557, 586
BsrI ACTGG 1 cut(s) 633
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 2 cut(s) 132, 163
BssNI GRCGYC 2 cut(s) 276, 423
BssT1I CCWWGG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 51, 511, 689
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 2 cut(s) 276, 423
BstC8I GCNNGC 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 361, 466
BstFNI CGCG 2 cut(s) 74, 197
BstHHI GCGC 3 cut(s) 76, 199, 617
BstKTI GATC 2 cut(s) 135, 166
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 2 cut(s) 132, 163
BstMCI CGRYCG 2 cut(s) 135, 283
BstMWI GCNNNNNNNGC 6 cut(s) 22, 205, 397, 406, 497, 559
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 2 cut(s) 74, 197
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuRI GGCC 1 cut(s) 400
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 361, 466
BtsIMutI CAGTG 1 cut(s) 516
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 393
CciI TCATGA 1 cut(s) 451
CfoI GCGC 3 cut(s) 76, 199, 617
Cfr13I GGNCC 5 cut(s) 250, 337, 398, 399, 667
CseI GACGC 2 cut(s) 203, 431
Csp6I GTAC 1 cut(s) 220
CviAII CATG 4 cut(s) 403, 433, 452, 619
CviQI GTAC 1 cut(s) 220
DpnI GATC 2 cut(s) 134, 165
DpnII GATC 2 cut(s) 132, 163
DrdI GACNNNNNNGTC 2 cut(s) 236, 248
DseDI GACNNNNNNGTC 2 cut(s) 236, 248
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 2 cut(s) 74, 173
Ecl136II GAGCTC 2 cut(s) 82, 409
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 3 cut(s) 84, 402, 411
Eco47I GGWCC 3 cut(s) 250, 337, 667
Eco53kI GAGCTC 2 cut(s) 82, 409
Eco57I CTGAAG 2 cut(s) 188, 533
EcoICRI GAGCTC 2 cut(s) 82, 409
EcoO109I RGGNCCY 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 3 cut(s) 84, 402, 411
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 406, 436, 455, 622
FaiI YATR 4 cut(s) 404, 434, 453, 620
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 402, 432, 451, 618
FblI GTMKAC 2 cut(s) 240, 279
Fnu4HI GCNGC 1 cut(s) 613
FokI GGATG 2 cut(s) 368, 473
FriOI GRGCYC 3 cut(s) 84, 402, 411
Fsp4HI GCNGC 1 cut(s) 613
GlaI GCGC 3 cut(s) 75, 198, 616
GluI GCNGC 1 cut(s) 613
GsaI CCCAGC 2 cut(s) 289, 399
GsuI CTGGAG 2 cut(s) 88, 290
HaeIII GGCC 1 cut(s) 400
HapII CCGG 2 cut(s) 77, 371
HgaI GACGC 2 cut(s) 203, 431
HhaI GCGC 3 cut(s) 76, 199, 617
Hin1I GRCGYC 2 cut(s) 276, 423
Hin1II CATG 4 cut(s) 406, 436, 455, 622
Hin6I GCGC 3 cut(s) 74, 197, 615
HinP1I GCGC 3 cut(s) 74, 197, 615
HincII GTYRAC 2 cut(s) 241, 280
HindII GTYRAC 2 cut(s) 241, 280
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 1 cut(s) 415
HpaII CCGG 2 cut(s) 77, 371
HphI GGTGA 2 cut(s) 493, 677
Hpy166II GTNNAC 2 cut(s) 241, 280
Hpy188I TCNGA 5 cut(s) 66, 118, 168, 355, 671
Hpy188III TCNNGA 8 cut(s) 105, 136, 269, 340, 412, 419, 452, 626
Hpy8I GTNNAC 2 cut(s) 241, 280
Hpy99I CGWCG 3 cut(s) 233, 281, 299
HpyAV CCTTC 6 cut(s) 118, 133, 163, 472, 488, 508
HpyCH4III ACNGT 3 cut(s) 51, 511, 689
HpyCH4IV ACGT 3 cut(s) 231, 276, 294
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 205, 397, 406, 497, 559
HpySE526I ACGT 3 cut(s) 231, 276, 294
Hsp92I GRCGYC 2 cut(s) 276, 423
Hsp92II CATG 4 cut(s) 406, 436, 455, 622
HspAI GCGC 3 cut(s) 74, 197, 615
Kzo9I GATC 2 cut(s) 132, 163
LmnI GCTCC 8 cut(s) 30, 79, 87, 385, 406, 450, 497, 558
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 1 cut(s) 451
MaeII ACGT 3 cut(s) 231, 276, 294
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 134, 165
MboI GATC 2 cut(s) 132, 163
MboII GAAGA 3 cut(s) 45, 487, 539
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 3 cut(s) 84, 402, 411
MluCI AATT 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 694
MspA1I CMGCKG 3 cut(s) 16, 285, 391
MspI CCGG 2 cut(s) 77, 371
Mva1269I GAATGC 1 cut(s) 208
MvnI CGCG 2 cut(s) 74, 197
MwoI GCNNNNNNNGC 6 cut(s) 22, 205, 397, 406, 497, 559
NcoI CCATGG 1 cut(s) 402
NdeII GATC 2 cut(s) 132, 163
NlaIII CATG 4 cut(s) 406, 436, 455, 622
NlaIV GGNNCC 2 cut(s) 381, 400
NmuCI GTSAC 1 cut(s) 505
PagI TCATGA 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 237
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 415
Pfl23II CGTACG 1 cut(s) 219
PkrI GCNGC 1 cut(s) 614
Ple19I CGATCG 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 250
Psp124BI GAGCTC 2 cut(s) 84, 411
Psp5II RGGWCCY 1 cut(s) 250
PspFI CCCAGC 2 cut(s) 285, 395
PspLI CGTACG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 381, 400
PspOMI GGGCCC 1 cut(s) 398
PspPI GGNCC 5 cut(s) 250, 337, 398, 399, 667
PspPPI RGGWCCY 1 cut(s) 250
PsuI RGATCY 1 cut(s) 163
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 2 cut(s) 84, 411
SalI GTCGAC 2 cut(s) 239, 278
SatI GCNGC 1 cut(s) 613
Sau3AI GATC 2 cut(s) 132, 163
Sau96I GGNCC 5 cut(s) 250, 337, 398, 399, 667
SduI GDGCHC 3 cut(s) 84, 402, 411
SfaNI GCATC 1 cut(s) 451
SinI GGWCC 3 cut(s) 250, 337, 667
Sse9I AATT 1 cut(s) 41
SsiI CCGC 6 cut(s) 14, 72, 85, 158, 283, 657
SstI GAGCTC 2 cut(s) 84, 411
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 3 cut(s) 51, 511, 689
TaiI ACGT 3 cut(s) 234, 279, 297
TaqI TCGA 7 cut(s) 183, 228, 240, 279, 297, 318, 627
TasI AATT 1 cut(s) 41
TfiI GAWTC 1 cut(s) 415
TscAI CASTG 1 cut(s) 516
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 1 cut(s) 612
Tsp45I GTSAC 1 cut(s) 505
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 1 cut(s) 516
VpaK11BI GGWCC 3 cut(s) 250, 337, 667
XmiI GTMKAC 2 cut(s) 240, 279
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.