pycom10g10140

5'-methylthioadenosine S-adenosylhomocysteine nucleosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010266
Physical Location & Seq
Forward (+)
13437902 .. 13441785
3884 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGCTCCTCACGGCGACAAATCGGACGCTACCGAGGAAGCCTTGGTTTCCGAAGCTGAGAAGCGCCCCATTTCCACCATCCTCCTCGTCATCGCTATGCAGACTGAAGCGCTTCCAGTGGTGAACAAGTTCAATCTCTCAGAGGATCCTGACTCCGTGTTCCCAAACGGGGTGCCTTGGGTTCGGTATCACGGTTCTTACAAAGATCTTCAGATTAATCTAGTTTGGCCTGGAAAAGATTCTTCTTTGGGGGTTGATTGTGTAGGCACAGTTTCTGCATCTCTTGTGACCTATGCTTCCATTCAAGCATTACAACCTGACCTAATTATCAATGCAGGCACTGCTGGTGGTTTTAAGGCCAAAGGAGCTTGCATCGGTGATGTGTATCTCGCATGTGATGTTGCTTACCATGACAGAAGGATACCTATTCCTGTCTTTGATCTATATGGAGTTGGTTTACGGCAAGCCTGCGCGTCACCGAATCTTCAAAAGGAACTTAATCTGAAGGTTGGTAAACTGTCCACTGGAAACTCTCTAGATATGTCCCCACAGGACGAAGCATCGATCATTGCAAATGATGCTACAATTAAAGACATGGAGGGGGCAGCTGTTGCTTACGTTGCAGATCTGTTGAAAGTCCCAATCATATTTGTTAAAGCCGTGACTGACATAGTAGACGGTGAAAAACCAACTGCTGAAGAATTCTTGAAAAATTTGGCAGCTGTAACTGCTGCTCTTGAAGAGAAGGTCACCCAAGTCATTGACTTTATTAATGGAAAGTGCCTCTCTGATCTTTGA

Protein Analysis

266

Amino Acids

28.25

Weight (kDa)

4.62

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 26 - 254 1.5e-28 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G38800
fragaria_vesca FvH4_2g02150
malus_domestica MD10G1116600.v1.1
prunus_persica Prupe.8G159400_v2.0.a1 Prupe.8G159500_v2.0.a1
pyrus_communis pycom10g10140
rosa_chinensis RchiOBHm_Chr6g0245651
rosa_laevigata RLG00000015296
rosa_multiflora Rmu_sc0016035.1_g000004
rosa_roxburghii Rroxscaffold_7G00215150
rosa_rugosa Rorug05G0515300
rosa_samantha Rh6AG030000 Rh6BG027400 Rh6CG026700 Rh6DG026100
rosa_wichuraiana Rw6G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 675
AccII CGCG 1 cut(s) 473
AclWI GGATC 2 cut(s) 140, 153
AcsI RAATTY 2 cut(s) 701, 712
AcuI CTGAAG 4 cut(s) 126, 194, 524, 717
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 6 cut(s) 133, 305, 488, 634, 709, 740
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 4 cut(s) 56, 368, 608, 722
AluI AGCT 4 cut(s) 56, 368, 608, 722
AlwI GGATC 2 cut(s) 140, 153
AlwNI CAGNNNCTG 2 cut(s) 275, 341
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 2 cut(s) 227, 357
ApeKI GCWGC 3 cut(s) 605, 719, 731
ApoI RAATTY 2 cut(s) 701, 712
AseI ATTAAT 2 cut(s) 216, 771
Asp700I GAANNNNTTC 3 cut(s) 111, 128, 238
AspLEI GCGC 3 cut(s) 66, 112, 473
AsuHPI GGTGA 5 cut(s) 133, 389, 468, 692, 742
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 172
BbvI GCAGC 3 cut(s) 617, 718, 731
BccI CCATC 1 cut(s) 86
BceAI ACGGC 3 cut(s) 28, 476, 644
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 414
BfaI CTAG 2 cut(s) 221, 536
BfoI RGCGCY 2 cut(s) 67, 113
BfuI GTATCC 1 cut(s) 414
BglII AGATCT 2 cut(s) 205, 625
BisI GCNGC 3 cut(s) 606, 720, 732
BlsI GCNGC 3 cut(s) 607, 721, 733
Bme1390I CCNGG 1 cut(s) 231
BmiI GGNNCC 3 cut(s) 6, 147, 174
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 4 cut(s) 287, 381, 568, 569
Bsa29I ATCGAT 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 3 cut(s) 33, 42, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 116, 529
Bse3DI GCAATG 1 cut(s) 567
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 33, 42, 176
BseGI GGATG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 2 cut(s) 48, 153
BseNI ACTGG 2 cut(s) 116, 529
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 3 cut(s) 617, 718, 731
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 2 cut(s) 229, 359
BshNI GGYRCC 1 cut(s) 172
BshVI ATCGAT 1 cut(s) 563
BslFI GGGAC 2 cut(s) 529, 623
BslI CCNNNNNNNGG 1 cut(s) 169
BsmFI GGGAC 2 cut(s) 529, 623
BsnI GGCC 2 cut(s) 229, 359
Bsp143I GATC 6 cut(s) 145, 205, 439, 564, 625, 790
BspANI GGCC 2 cut(s) 229, 359
BspCNI CTCAG 2 cut(s) 49, 152
BspDI ATCGAT 1 cut(s) 563
BspFNI CGCG 1 cut(s) 473
BspLI GGNNCC 3 cut(s) 6, 147, 174
BspPI GGATC 2 cut(s) 140, 153
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 2 cut(s) 116, 529
BssECI CCNNGG 3 cut(s) 33, 42, 176
BssMI GATC 6 cut(s) 145, 205, 439, 564, 625, 790
BssT1I CCWWGG 2 cut(s) 42, 176
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 4 cut(s) 194, 271, 519, 680
Bst6I CTCTTC 1 cut(s) 735
BstAPI GCANNNNNTGC 3 cut(s) 341, 578, 611
BstC8I GCNNGC 4 cut(s) 337, 370, 465, 469
BstDEI CTNAG 2 cut(s) 57, 139
BstEII GGTNACC 1 cut(s) 748
BstF5I GGATG 1 cut(s) 78
BstFNI CGCG 1 cut(s) 473
BstH2I RGCGCY 2 cut(s) 67, 113
BstHHI GCGC 3 cut(s) 66, 112, 473
BstKTI GATC 6 cut(s) 148, 208, 442, 567, 628, 793
BstMBI GATC 6 cut(s) 145, 205, 439, 564, 625, 790
BstMWI GCNNNNNNNGC 6 cut(s) 341, 365, 578, 611, 620, 728
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 396
BstPI GGTNACC 1 cut(s) 748
BstSCI CCNGG 1 cut(s) 229
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 3 cut(s) 617, 718, 731
BstX2I RGATCY 3 cut(s) 145, 205, 625
BstYI RGATCY 3 cut(s) 145, 205, 625
Bsu15I ATCGAT 1 cut(s) 563
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 229, 359
BsuTUI ATCGAT 1 cut(s) 563
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 123, 339, 522
Cac8I GCNNGC 4 cut(s) 337, 370, 465, 469
CaiI CAGNNNCTG 2 cut(s) 275, 341
CfoI GCGC 3 cut(s) 66, 112, 473
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 2 cut(s) 35, 462
CviAII CATG 3 cut(s) 393, 410, 595
DdeI CTNAG 2 cut(s) 57, 139
DpnI GATC 6 cut(s) 147, 207, 441, 566, 627, 792
DpnII GATC 6 cut(s) 145, 205, 439, 564, 625, 790
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco130I CCWWGG 2 cut(s) 42, 176
Eco47III AGCGCT 1 cut(s) 111
Eco57I CTGAAG 4 cut(s) 126, 194, 524, 717
Eco91I GGTNACC 1 cut(s) 748
EcoO65I GGTNACC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 42, 176
ErhI CCWWGG 2 cut(s) 42, 176
FaeI CATG 3 cut(s) 396, 413, 598
FaqI GGGAC 2 cut(s) 529, 623
FatI CATG 3 cut(s) 392, 409, 594
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 606, 720, 732
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 606, 720, 732
FspBI CTAG 2 cut(s) 221, 536
GlaI GCGC 3 cut(s) 65, 111, 472
GluI GCNGC 3 cut(s) 606, 720, 732
HaeII RGCGCY 2 cut(s) 67, 113
HaeIII GGCC 2 cut(s) 229, 359
HgaI GACGC 2 cut(s) 35, 462
HhaI GCGC 3 cut(s) 66, 112, 473
Hin1II CATG 3 cut(s) 396, 413, 598
Hin6I GCGC 3 cut(s) 64, 110, 471
HinP1I GCGC 3 cut(s) 64, 110, 471
HinfI GANTC 3 cut(s) 152, 239, 481
HphI GGTGA 5 cut(s) 133, 389, 468, 692, 742
Hpy166II GTNNAC 5 cut(s) 124, 458, 515, 522, 676
Hpy188I TCNGA 6 cut(s) 25, 52, 142, 213, 504, 790
Hpy188III TCNNGA 4 cut(s) 149, 536, 706, 737
Hpy8I GTNNAC 5 cut(s) 124, 458, 515, 522, 676
HpyAV CCTTC 3 cut(s) 411, 499, 739
HpyCH4III ACNGT 4 cut(s) 194, 271, 519, 680
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 6 cut(s) 100, 278, 335, 372, 572, 623
HpyF10VI GCNNNNNNNGC 6 cut(s) 341, 365, 578, 611, 620, 728
HpyF3I CTNAG 2 cut(s) 57, 139
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 3 cut(s) 396, 413, 598
HspAI GCGC 3 cut(s) 64, 110, 471
Kzo9I GATC 6 cut(s) 145, 205, 439, 564, 625, 790
LmnI GCTCC 2 cut(s) 10, 365
Lsp1109I GCAGC 3 cut(s) 617, 718, 731
LweI GCATC 4 cut(s) 287, 381, 568, 569
MaeI CTAG 2 cut(s) 221, 536
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 5 cut(s) 286, 474, 661, 724, 748
MalI GATC 6 cut(s) 147, 207, 441, 566, 627, 792
MboI GATC 6 cut(s) 145, 205, 439, 564, 625, 790
MboII GAAGA 5 cut(s) 200, 234, 476, 710, 752
MflI RGATCY 3 cut(s) 145, 205, 625
MluCI AATT 4 cut(s) 324, 585, 701, 712
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 7 cut(s) 18, 28, 92, 95, 136, 592, 794
MroXI GAANNNNTTC 3 cut(s) 111, 128, 238
MseI TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 608, 722
MspR9I CCNGG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 6 cut(s) 341, 365, 578, 611, 620, 728
NdeII GATC 6 cut(s) 145, 205, 439, 564, 625, 790
NlaIII CATG 3 cut(s) 396, 413, 598
NlaIV GGNNCC 3 cut(s) 6, 147, 174
NmuCI GTSAC 4 cut(s) 286, 474, 661, 748
NspI RCATGY 1 cut(s) 396
PdmI GAANNNNTTC 3 cut(s) 111, 128, 238
PfeI GAWTC 2 cut(s) 239, 481
PkrI GCNGC 3 cut(s) 607, 721, 733
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PshBI ATTAAT 2 cut(s) 216, 771
Psp6I CCWGG 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 748
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 6, 147, 174
PstNI CAGNNNCTG 2 cut(s) 275, 341
PsuI RGATCY 3 cut(s) 145, 205, 625
PvuII CAGCTG 2 cut(s) 608, 722
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
SatI GCNGC 3 cut(s) 606, 720, 732
Sau3AI GATC 6 cut(s) 145, 205, 439, 564, 625, 790
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 231
SfaNI GCATC 4 cut(s) 287, 381, 568, 569
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 4 cut(s) 324, 585, 701, 712
SspMI CTAG 2 cut(s) 221, 536
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 2 cut(s) 42, 176
TaaI ACNGT 4 cut(s) 194, 271, 519, 680
TaiI ACGT 1 cut(s) 621
TaqI TCGA 1 cut(s) 563
TasI AATT 4 cut(s) 324, 585, 701, 712
TfiI GAWTC 2 cut(s) 239, 481
Tru1I TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
Tru9I TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
TscAI CASTG 3 cut(s) 123, 346, 529
TseFI GTSAC 4 cut(s) 286, 474, 661, 748
TseI GCWGC 3 cut(s) 605, 719, 731
Tsp45I GTSAC 4 cut(s) 286, 474, 661, 748
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 3 cut(s) 123, 346, 529
VspI ATTAAT 2 cut(s) 216, 771
XapI RAATTY 2 cut(s) 701, 712
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 3 cut(s) 111, 128, 238
XspI CTAG 2 cut(s) 221, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.