pycom10g10550

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009560
Physical Location & Seq
Forward (+)
13932942 .. 13935984
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1776 bp
ATGGACAGCTCCGAAGAACCCAACAAGCCCATCTTCTACGACTCATCCTCTCCCTCCACCCCCCTCCTCTCCAAACCCCCAATCGAAGACCCACCACCGCCACCGTCCCCTCCGCCATCTTACACCGACCCCCCGGAATCCGATACCGACCCGACCCAGTATCTCCAGATCTCCTACAACCACGGCCCCCGCCATTTCAAAGACCTCCCCTTTCTAATCCTTCACCTCCTCTTTATCCTGGCCACCTTCGCCATTGGAATCTTCGCCGTCGTCCACCGCAACCAAAATTACACCGCCCTCTCCTCCTTCTCCTACGACTCCGACTCCGCCTCCTGCGTCAAAAACGAAACTTTGACCCGTCTCCCCAACTGGGTCGGACCCACTTTCTCCTCTTCCTCCTCTCCGCTCAAAGATTTGATATGGACCCTCGTGATTACGTTGATTCTAAGCCTGCCCATTTGCTTCAGTCTGCTGATTCTCCTCAAACACTACGCCAAGCAAGTCGTCTACGTCTTCCTCCCCTTCTTCATCCTCTTCCCAATCTTCTTCAACGTCTACTGGTTCGTCGCCTGCACTCTCAGCTCCACCTGCAGCGACGCTCTCCCTTTAGTTTACAGAATTTTGATTCTGATTTTCGTGTTTTTGGTAATTGGGGTCATTGTGTGGATTCTTGTGGTGAATTGGCATCGGATTGAGCTCACTGTGAGCATAATCGGGGTGGCATCCCATGCGCTTTCCCGGAACTTGGGCTTGTTCGGGGTTCTGCCGTGTTTGACACTAGGATTGGTGGTTTACTATGCGCCCATTGTTGTGTTCTTGGTCTATGCCAGGTTCAATGGGAAGATTGCACCGCGGGAATCGAATGGAGTGTATAGTTGTGTGTGGAAGCAGGATGGTTGGGTGCCTGCTTATTACGCATTGGCGATTTTGACAATGTTGTGGTCGTTTACGACGATGGTGGAAGCTCAGGTGTATGTGATTAGTGGGACTGTTGCTCAGTGGTATTTCACTAAGGAGGATTCAACTCCGAAGCGGATTATCAGGAGCTCTTTAAGAAATGCCTTTGGCCCCAACTTTGGCACAGTATGCCTATCTGGATTACTTTTATCTGTTGTTCGAGTTGTTCGTGCCATGGTTGATGGTGCAAGACAAGACGGTTCACCTGGGATGGTGTATTTTGTAATGCGCTGTTGTGTTAATGCCTTATTGTCAGCGATTGATTTTCTCAACAAGTTCACAATCAACTTTGCGGCAATAACTGGTGAAGCTTACTGCTCATCTGCAAAAATGACGTATGAGCTTCTAAAGCGCAATCTTCTCTCGGCGGTTTTTGTGGAAACCATCTCCACTCGTCTATTGGCTGGAATTGTTTTTGTACTCTCAGCAATATATGCAATTGTGGTCTGCGCTATCTTACAAGCTGCGACCAGTCTTGGGGTTGATTCGTACTTTGTGGCAGTTCTGGCGTGGGTGCTGCTGATCTTGGTACTGGGTTTCTTTGTTCATGTGCTGGACAATGTAATTGACACCGTGTACGTGTGCTACGCCATAGATAGGGACAGAGGAGAGGTTTGTAAACAGGAAGTTCACGAGGTTTACACTCACTTACCCATTAGTCGAAACCATAGACCATCTTCTATGATCGATCCACAACTCTGGGTGTATAAATTAATCTTTGATTTACAATGTTGTGGTGTTGATTTGTTCTTTGTGTCAAGTTGGAATTGCTTGCCGGTAGATTGTGCATATCGAGTGTTTCTTTGTAACGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

592

Amino Acids

66.21

Weight (kDa)

6.41

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 211 - 520 1.3e-68 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 901
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 2 cut(s) 507, 555
AccII CGCG 1 cut(s) 853
AclWI GGATC 1 cut(s) 1640
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 618
AcuI CTGAAG 1 cut(s) 448
AfaI GTAC 4 cut(s) 1377, 1448, 1488, 1535
AfiI CCNNNNNNNGG 5 cut(s) 370, 745, 1076, 1434, 1554
AflIII ACRYGT 1 cut(s) 1536
AgsI TTSAA 4 cut(s) 199, 550, 835, 1023
AjnI CCWGG 3 cut(s) 237, 827, 1162
AluBI AGCT 8 cut(s) 9, 582, 697, 965, 1047, 1268, 1300, 1421
AluI AGCT 8 cut(s) 9, 582, 697, 965, 1047, 1268, 1300, 1421
Alw21I GWGCWC 2 cut(s) 699, 1049
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 1640
AoxI GGCC 3 cut(s) 184, 240, 1066
ApeKI GCWGC 3 cut(s) 591, 1421, 1474
ApoI RAATTY 1 cut(s) 618
AseI ATTAAT 1 cut(s) 1670
AspLEI GCGC 5 cut(s) 733, 802, 1188, 1311, 1409
AspS9I GGNCC 4 cut(s) 185, 377, 423, 1067
AsuC2I CCSGG 2 cut(s) 134, 739
AsuHPI GGTGA 4 cut(s) 215, 688, 1152, 1274
AvaII GGWCC 2 cut(s) 377, 423
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 1 cut(s) 901
BanII GRGCYC 2 cut(s) 699, 1049
BauI CACGAG 2 cut(s) 428, 1589
BbsI GAAGAC 2 cut(s) 93, 505
Bbv12I GWGCWC 2 cut(s) 699, 1049
BbvI GCAGC 3 cut(s) 603, 1408, 1461
BccI CCATC 8 cut(s) 38, 124, 887, 949, 1133, 1162, 1349, 1639
BceAI ACGGC 3 cut(s) 199, 251, 751
BciT130I CCWGG 3 cut(s) 239, 829, 1164
BcnI CCSGG 2 cut(s) 134, 739
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 779
BfmI CTRYAG 1 cut(s) 589
BfuAI ACCTGC 1 cut(s) 596
BglII AGATCT 1 cut(s) 168
BisI GCNGC 4 cut(s) 592, 1251, 1422, 1475
BlsI GCNGC 4 cut(s) 593, 1252, 1423, 1476
Bme1390I CCNGG 5 cut(s) 134, 239, 739, 829, 1164
Bme18I GGWCC 2 cut(s) 377, 423
BmgT120I GGNCC 4 cut(s) 185, 377, 423, 1067
BmiI GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
BmrFI CCNGG 5 cut(s) 134, 239, 739, 829, 1164
BmrI ACTGGG 3 cut(s) 151, 379, 1499
BmsI GCATC 2 cut(s) 694, 731
BmuI ACTGGG 3 cut(s) 151, 379, 1499
BpiI GAAGAC 2 cut(s) 93, 505
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 966
BpuMI CCSGG 2 cut(s) 134, 739
Bsa29I ATCGAT 1 cut(s) 1644
BsaAI YACGTR 1 cut(s) 1537
BsaJI CCNNGG 5 cut(s) 132, 181, 851, 1131, 1163
BsaXI ACNNNNNCTCC 4 cut(s) 308, 314, 338, 344
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 745, 1076, 1434, 1554
Bse118I RCCGGY 1 cut(s) 1732
Bse1I ACTGG 6 cut(s) 157, 374, 563, 1264, 1428, 1494
BseBI CCWGG 3 cut(s) 239, 829, 1164
BseCI ATCGAT 1 cut(s) 1644
BseDI CCNNGG 5 cut(s) 132, 181, 851, 1131, 1163
BseGI GGATG 5 cut(s) 44, 528, 722, 898, 1173
BseLI CCNNNNNNNGG 5 cut(s) 370, 745, 1076, 1434, 1554
BseMII CTCAG 4 cut(s) 592, 980, 1010, 1395
BseNI ACTGG 6 cut(s) 157, 374, 563, 1264, 1428, 1494
BseRI GAGGAG 7 cut(s) 56, 218, 292, 379, 388, 470, 1578
BseXI GCAGC 3 cut(s) 603, 1408, 1461
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 853
BshFI GGCC 3 cut(s) 186, 242, 1068
BshNI GGYRCC 1 cut(s) 901
BshVI ATCGAT 1 cut(s) 1644
BsiHKAI GWGCWC 2 cut(s) 699, 1049
BsiSI CCGG 3 cut(s) 134, 739, 1733
BslFI GGGAC 3 cut(s) 91, 1000, 1571
BslI CCNNNNNNNGG 5 cut(s) 370, 745, 1076, 1434, 1554
BsmAI GTCTC 1 cut(s) 365
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 3 cut(s) 91, 1000, 1571
BsnI GGCC 3 cut(s) 186, 242, 1068
Bsp1286I GDGCHC 2 cut(s) 699, 1049
Bsp143I GATC 4 cut(s) 168, 1479, 1641, 1645
Bsp19I CCATGG 1 cut(s) 1131
BspANI GGCC 3 cut(s) 186, 242, 1068
BspCNI CTCAG 4 cut(s) 591, 979, 1009, 1394
BspDI ATCGAT 1 cut(s) 1644
BspFNI CGCG 1 cut(s) 853
BspLI GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
BspMAI CTGCAG 1 cut(s) 593
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 1 cut(s) 1640
BspT107I GGYRCC 1 cut(s) 901
BsrBI CCGCTC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 1732
BsrI ACTGG 6 cut(s) 157, 374, 563, 1264, 1428, 1494
BssAI RCCGGY 1 cut(s) 1732
BssECI CCNNGG 5 cut(s) 132, 181, 851, 1131, 1163
BssMI GATC 4 cut(s) 168, 1479, 1641, 1645
BssSI CACGAG 2 cut(s) 428, 1589
BssT1I CCWWGG 1 cut(s) 1131
Bst2BI CACGAG 2 cut(s) 428, 1589
Bst2UI CCWGG 3 cut(s) 239, 829, 1164
Bst4CI ACNGT 6 cut(s) 105, 703, 991, 1084, 1157, 1531
Bst6I CTCTTC 2 cut(s) 397, 539
BstAPI GCANNNNNTGC 3 cut(s) 728, 1086, 1391
BstBAI YACGTR 1 cut(s) 1537
BstC8I GCNNGC 4 cut(s) 452, 571, 906, 1730
BstDEI CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
BstDSI CCRYGG 3 cut(s) 181, 851, 1131
BstF5I GGATG 5 cut(s) 44, 528, 722, 898, 1173
BstFNI CGCG 1 cut(s) 853
BstHHI GCGC 5 cut(s) 733, 802, 1188, 1311, 1409
BstKTI GATC 4 cut(s) 171, 1482, 1644, 1648
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 4 cut(s) 168, 1479, 1641, 1645
BstMWI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
BstNI CCWGG 3 cut(s) 239, 829, 1164
BstSCI CCNGG 5 cut(s) 132, 237, 737, 827, 1162
BstSFI CTRYAG 1 cut(s) 589
BstUI CGCG 1 cut(s) 853
BstV1I GCAGC 3 cut(s) 603, 1408, 1461
BstV2I GAAGAC 2 cut(s) 93, 505
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 1656
BstYI RGATCY 1 cut(s) 168
Bsu15I ATCGAT 1 cut(s) 1644
BsuRI GGCC 3 cut(s) 186, 242, 1068
BsuTUI ATCGAT 1 cut(s) 1644
BtgI CCRYGG 3 cut(s) 181, 851, 1131
BtsCI GGATG 5 cut(s) 44, 528, 722, 898, 1173
BtsIMutI CAGTG 2 cut(s) 699, 1004
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 452, 571, 906, 1730
CfoI GCGC 5 cut(s) 733, 802, 1188, 1311, 1409
Cfr10I RCCGGY 1 cut(s) 1732
Cfr13I GGNCC 4 cut(s) 185, 377, 423, 1067
Cfr42I CCGCGG 1 cut(s) 854
ClaI ATCGAT 1 cut(s) 1644
CseI GACGC 2 cut(s) 325, 605
Csp6I GTAC 4 cut(s) 1376, 1447, 1487, 1534
CviAII CATG 3 cut(s) 728, 1132, 1505
CviQI GTAC 4 cut(s) 1376, 1447, 1487, 1534
DdeI CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
DpnI GATC 4 cut(s) 170, 1481, 1643, 1647
DpnII GATC 4 cut(s) 168, 1479, 1641, 1645
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 397, 539
EarI CTCTTC 2 cut(s) 397, 539
EciI GGCGGA 2 cut(s) 102, 316
Ecl136II GAGCTC 2 cut(s) 697, 1047
Eco130I CCWWGG 1 cut(s) 1131
Eco24I GRGCYC 2 cut(s) 699, 1049
Eco47I GGWCC 2 cut(s) 377, 423
Eco53kI GAGCTC 2 cut(s) 697, 1047
Eco57I CTGAAG 1 cut(s) 448
EcoICRI GAGCTC 2 cut(s) 697, 1047
EcoRII CCWGG 3 cut(s) 237, 827, 1162
EcoT14I CCWWGG 1 cut(s) 1131
EcoT38I GRGCYC 2 cut(s) 699, 1049
ErhI CCWWGG 1 cut(s) 1131
Esp3I CGTCTC 1 cut(s) 365
FaeI CATG 3 cut(s) 731, 1135, 1508
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 3 cut(s) 91, 1000, 1571
FatI CATG 3 cut(s) 727, 1131, 1504
FauI CCCGC 2 cut(s) 197, 846
FblI GTMKAC 2 cut(s) 507, 555
Fnu4HI GCNGC 4 cut(s) 592, 1251, 1422, 1475
FokI GGATG 5 cut(s) 31, 515, 709, 905, 1180
FriOI GRGCYC 2 cut(s) 699, 1049
Fsp4HI GCNGC 4 cut(s) 592, 1251, 1422, 1475
FspBI CTAG 1 cut(s) 779
GlaI GCGC 5 cut(s) 732, 801, 1187, 1310, 1408
GluI GCNGC 4 cut(s) 592, 1251, 1422, 1475
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 3 cut(s) 186, 242, 1068
HapII CCGG 3 cut(s) 134, 739, 1733
HgaI GACGC 2 cut(s) 325, 605
HhaI GCGC 5 cut(s) 733, 802, 1188, 1311, 1409
Hin1II CATG 3 cut(s) 731, 1135, 1508
Hin6I GCGC 5 cut(s) 731, 800, 1186, 1309, 1407
HinP1I GCGC 5 cut(s) 731, 800, 1186, 1309, 1407
HindIII AAGCTT 1 cut(s) 1266
HpaII CCGG 3 cut(s) 134, 739, 1733
HphI GGTGA 4 cut(s) 215, 688, 1152, 1274
Hpy188I TCNGA 8 cut(s) 13, 142, 322, 377, 630, 690, 1029, 1775
Hpy188III TCNNGA 5 cut(s) 166, 430, 1042, 1095, 1589
Hpy99I CGWCG 4 cut(s) 272, 569, 599, 955
HpyAV CCTTC 4 cut(s) 230, 256, 316, 532
HpyCH4III ACNGT 6 cut(s) 105, 703, 991, 1084, 1157, 1531
HpyCH4IV ACGT 5 cut(s) 437, 510, 552, 1292, 1536
HpyCH4V TGCA 7 cut(s) 573, 591, 848, 1145, 1283, 1394, 1745
HpyF10VI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
HpyF3I CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
HpySE526I ACGT 5 cut(s) 437, 510, 552, 1292, 1536
Hsp92II CATG 3 cut(s) 731, 1135, 1508
HspAI GCGC 5 cut(s) 731, 800, 1186, 1309, 1407
KspI CCGCGG 1 cut(s) 854
Kzo9I GATC 4 cut(s) 168, 1479, 1641, 1645
LmnI GCTCC 3 cut(s) 14, 587, 1044
Lsp1109I GCAGC 3 cut(s) 603, 1408, 1461
LweI GCATC 2 cut(s) 694, 731
MaeI CTAG 1 cut(s) 779
MaeII ACGT 5 cut(s) 437, 510, 552, 1292, 1536
MaeIII GTNAC 1 cut(s) 1763
MalI GATC 4 cut(s) 170, 1481, 1643, 1647
MbiI CCGCTC 1 cut(s) 406
MboI GATC 4 cut(s) 168, 1479, 1641, 1645
MfeI CAATTG 1 cut(s) 1395
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 699, 1049
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 9 cut(s) 286, 618, 648, 679, 1365, 1395, 1521, 1667, 1723
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 3 cut(s) 35, 311, 317
MmeI TCCRAC 3 cut(s) 345, 355, 1700
Mox20I TGGCCA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 242
MseI TTAA 3 cut(s) 1052, 1197, 1670
MslI CAYNNNNRTG 1 cut(s) 809
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 3 cut(s) 134, 739, 1733
MspR9I CCNGG 5 cut(s) 134, 239, 739, 829, 1164
MunI CAATTG 1 cut(s) 1395
MvaI CCWGG 3 cut(s) 239, 829, 1164
MvnI CGCG 1 cut(s) 853
MwoI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
NciI CCSGG 2 cut(s) 134, 739
NcoI CCATGG 1 cut(s) 1131
NdeII GATC 4 cut(s) 168, 1479, 1641, 1645
NlaIII CATG 3 cut(s) 731, 1135, 1508
NlaIV GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
NmeAIII GCCGAG 1 cut(s) 1301
PaqCI CACCTGC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 2 cut(s) 950, 1123
PfeI GAWTC 9 cut(s) 137, 258, 442, 475, 625, 667, 857, 1019, 1442
PfoI TCCNGGA 1 cut(s) 737
PkrI GCNGC 4 cut(s) 593, 1252, 1423, 1476
PleI GAGTC 3 cut(s) 35, 311, 317
PpsI GAGTC 3 cut(s) 35, 311, 317
Ppu21I YACGTR 1 cut(s) 1537
PshBI ATTAAT 1 cut(s) 1670
Psp124BI GAGCTC 2 cut(s) 699, 1049
Psp6I CCWGG 3 cut(s) 237, 827, 1162
PspGI CCWGG 3 cut(s) 237, 827, 1162
PspN4I GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
PspPI GGNCC 4 cut(s) 185, 377, 423, 1067
PstI CTGCAG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 4 cut(s) 1377, 1448, 1488, 1535
RsaNI GTAC 4 cut(s) 1376, 1447, 1487, 1534
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 2 cut(s) 699, 1049
SacII CCGCGG 1 cut(s) 854
SaqAI TTAA 3 cut(s) 1052, 1197, 1670
SatI GCNGC 4 cut(s) 592, 1251, 1422, 1475
Sau3AI GATC 4 cut(s) 168, 1479, 1641, 1645
Sau96I GGNCC 4 cut(s) 185, 377, 423, 1067
SchI GAGTC 3 cut(s) 35, 311, 317
ScrFI CCNGG 5 cut(s) 134, 239, 739, 829, 1164
SduI GDGCHC 2 cut(s) 699, 1049
SfaNI GCATC 2 cut(s) 694, 731
SfcI CTRYAG 1 cut(s) 589
Sfr303I CCGCGG 1 cut(s) 854
SgrBI CCGCGG 1 cut(s) 854
SinI GGWCC 2 cut(s) 377, 423
SmiMI CAYNNNNRTG 1 cut(s) 809
Sse9I AATT 9 cut(s) 286, 618, 648, 679, 1365, 1395, 1521, 1667, 1723
SspMI CTAG 1 cut(s) 779
SstI GAGCTC 2 cut(s) 699, 1049
StyD4I CCNGG 5 cut(s) 132, 237, 737, 827, 1162
StyI CCWWGG 1 cut(s) 1131
TaaI ACNGT 6 cut(s) 105, 703, 991, 1084, 1157, 1531
TaiI ACGT 5 cut(s) 440, 513, 555, 1295, 1539
TaqI TCGA 6 cut(s) 84, 860, 1117, 1618, 1644, 1750
TasI AATT 9 cut(s) 286, 618, 648, 679, 1365, 1395, 1521, 1667, 1723
TatI WGTACW 1 cut(s) 1375
TauI GCSGC 1 cut(s) 1253
TfiI GAWTC 9 cut(s) 137, 258, 442, 475, 625, 667, 857, 1019, 1442
Tru1I TTAA 3 cut(s) 1052, 1197, 1670
Tru9I TTAA 3 cut(s) 1052, 1197, 1670
TscAI CASTG 2 cut(s) 706, 1004
TseI GCWGC 3 cut(s) 591, 1421, 1474
TspDTI ATGAA 2 cut(s) 517, 1493
TspRI CASTG 2 cut(s) 706, 1004
VpaK11BI GGWCC 2 cut(s) 377, 423
VspI ATTAAT 1 cut(s) 1670
XapI RAATTY 1 cut(s) 618
XcmI CCANNNNNNNNNTGG 1 cut(s) 1354
XmiI GTMKAC 2 cut(s) 507, 555
XspI CTAG 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.