pycom10g14130

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008250
Physical Location & Seq
Reverse (-)
17533110 .. 17535328
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1974 bp
ATGTCTTTTGCTTGCCAAGAAGTTCAGCCATGGACCCTTGCTGGTCTTATACGAGCTTTTCTCGACCTGGGTTTGGCGTATTTCCTGCTGTGTGTGTCTGCTTTCATGTTTTTCGCTTCGAAGTGGTTGAGGATTTTTTGGGTTTATTTGCCCTGTCCTTGTAATGGGGTTTTTGGGTATCGAAACCGTGATCTTTGCTTGCATAAGTTGCTTATTGACCGGCCTGTTGGGACGATCCACGATGTTCACAAGCTGGTGAAGAGCAGGTTCCCTTTTGATGTTATTTGGTCTCCGGATCAAAGATGCAACTTGAATAGGAAACTGATCGGAGATGTGAATTGTGAGAATGGACTCCATGAGTTGGAAGGTGAAGCGGATTCTAGTCCGTTTTCGAGTCCCAGATTGCAAAACCTGGTTGATAGGGAAAGTGGGTATGATGCTAAGGGGAAGAGGGTAATGACTCTCAAGAAAAGGGCTGGAATTCGGCGTTCTAGGAGAGCGGCTCCTGAATACGGAAGGCTTTCTTCTTACTATACAAGTGATAGTTCAAGGTTGGTTGCTCGCATATTCCCCTTTCCTTGCGATGACAGAAATACAAGGGAGATGTCGGGTGAAAGCTCAGTTGCCATAGCTGGTAGAGAAGATTATGTGAAAGTATCAACTGGAGATGATGTGGCTGAAAGTATGTGCCAAAGTTCTGAATTGAGTGGATTATTTGATGCGAGTAAGGACGTGGATGTTAGTAACACACAAGAAAAGGCTCCCGTTGTTGGAAATGAAACTGATAAACTCAGGGTGTTGGAAGAAGCACTGGAAAAAGAGAAAGCTGCTTGTGCTACTCTTTATCTAGAGCTAGAGAAAGAGCGAGCTGCTGCTGCAAGTGCTGCTGATGAAGCGATGGCCATGATAGCCCGTCTGCAAAAGGATAAAGCATCCATAGAAATGGAAGTGAGGCAGTACCAAAGGATGATCGATCCATCAGAAAAATTTGTTTATGATGAAGAGGAGATGGATGCTCTGAAAGAGATTCTTCTTAGGAGGGAGATGGAGAACCATTTTTTGGAGAAGGAAGTTGAATCGTATAGGCAGATGAGCATCTTAGGAAATGAACGGTCTGATGCTGCTTTGAGTGATATGTTGTGTGAACCGGGAAAAACGCCTTCACCTTCACTTGATCCAAATACAGATGCACAACTGATGCTAAAGCAGACTGCTGATAGTAAATCCAACTGTACGGCAATCGAAAATATTGCAAATAGTACTTCTCAGTATGAGGCTTCATTTGTTGAAAAACAAATCCATCCTAATGAACATGATTCACTTGAGAAATGTGTACTTTCAGAAAGGGGAGAAAAAGTACACATGGATAATGTAATGTGCCAAGAAATGACTACTGAAGCAGCCCAAGCTCATATTGGTACCGAGGAAAACTTACATTGTGATGAAGAAGTGCCACAGAGTGATGGGGATCTTCAGAGGAATTTGCATGGTTCAATGCTTGATATTGAGCCAGCTGTTTATGATGTTCATGTCGTTGATGGTAAAACTGAACTTTGGAAAGAAGAAGGTAGATCATCACTTTATACTGCATTGGATGGATCCCAAGATTTCTCTCATACATTTGGCGCTGCCGGGGTCAGTGGCAGTGAGTTATTACAAGGTTTTCCAAGTACAAGCCAGGTGGATACTGAACCATTTATTCCAAGTTCTTTGGATATGCATTGTGGGTTACCGATGTTGGATAGCTCCCGATGCAAAACCTTGGTGATAGACTCAATGAAGAACTCATTGTCCACAGTAAATAGTGAAAGGTCGAAGATTGACACTGAGGTTGAATTGCTAATGGAAAAGCTACAGATTCTAGAAGAGAGAAAAGAGAAGTTGACTTCCTCTTCTGAACATGGGGAGAGGCAAAAAACCCCATCAAAATGTTTGGAGGAGATCGGAACCCACTCTGCAAGATTCAGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

658

Amino Acids

73.76

Weight (kDa)

4.99

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 267 - 358 3.9e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
Acc65I GGTACC 1 cut(s) 1418
AccB1I GGYRCC 1 cut(s) 1418
AccB7I CCANNNNNTGG 2 cut(s) 361, 1060
AccBSI CCGCTC 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 2 cut(s) 374, 500
AclWI GGATC 7 cut(s) 229, 303, 968, 1169, 1476, 1593, 1606
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 3 cut(s) 480, 986, 1480
AcuI CTGAAG 2 cut(s) 1416, 1457
AdeI CACNNNGTG 1 cut(s) 1460
AfaI GTAC 7 cut(s) 959, 1234, 1261, 1335, 1359, 1420, 1672
AfiI CCNNNNNNNGG 6 cut(s) 73, 164, 361, 512, 770, 1060
AgsI TTSAA 6 cut(s) 313, 549, 1076, 1289, 1494, 1835
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 3 cut(s) 66, 411, 1677
AjuI GAANNNNNNNTTGG 2 cut(s) 1043, 1075
AloI GAACNNNNNNTCC 2 cut(s) 1775, 1807
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 7 cut(s) 229, 303, 968, 1169, 1476, 1593, 1606
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 2 cut(s) 221, 900
ApeKI GCWGC 8 cut(s) 827, 869, 872, 875, 884, 1121, 1400, 1628
ApoI RAATTY 3 cut(s) 480, 986, 1480
Asp700I GAANNNNTTC 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 1418
AspLEI GCGC 1 cut(s) 1628
AspS9I GGNCC 1 cut(s) 33
AsuC2I CCSGG 2 cut(s) 1149, 1633
AsuHPI GGTGA 5 cut(s) 268, 380, 623, 1155, 1777
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 902
BamHI GGATCC 1 cut(s) 1598
BanI GGYRCC 1 cut(s) 1418
BbvI GCAGC 8 cut(s) 814, 856, 859, 862, 871, 1108, 1412, 1615
BccI CCATC 9 cut(s) 892, 985, 1003, 1039, 1308, 1457, 1532, 1589, 1930
BceAI ACGGC 1 cut(s) 1251
BcgI CGANNNNNNTGC 2 cut(s) 1232, 1266
BciT130I CCWGG 3 cut(s) 68, 413, 1679
BciVI GTATCC 1 cut(s) 1678
BcnI CCSGG 2 cut(s) 1149, 1633
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 5 cut(s) 381, 492, 848, 854, 1862
BfmI CTRYAG 1 cut(s) 1853
BfoI RGCGCY 1 cut(s) 1629
BfuAI ACCTGC 1 cut(s) 255
BfuI GTATCC 1 cut(s) 1678
BisI GCNGC 9 cut(s) 501, 828, 870, 873, 876, 885, 1122, 1401, 1629
BlsI GCNGC 9 cut(s) 502, 829, 871, 874, 877, 886, 1123, 1402, 1630
BmcAI AGTACT 1 cut(s) 1261
Bme1390I CCNGG 5 cut(s) 68, 413, 1149, 1633, 1679
Bme18I GGWCC 1 cut(s) 33
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 7 cut(s) 35, 269, 504, 762, 1420, 1600, 1948
BmrFI CCNGG 5 cut(s) 68, 413, 1149, 1633, 1679
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 487, 519
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 449, 1343
BpuMI CCSGG 2 cut(s) 1149, 1633
Bsa29I ATCGAT 1 cut(s) 972
BsaBI GATNNNNATC 2 cut(s) 1094, 1467
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 5 cut(s) 29, 67, 1422, 1632, 1761
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 4 cut(s) 657, 687, 1341, 1371
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 164, 361, 512, 770, 1060
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 667, 816
Bse8I GATNNNNATC 2 cut(s) 1094, 1467
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 3 cut(s) 68, 413, 1679
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 5 cut(s) 29, 67, 1422, 1632, 1761
BseGI GGATG 6 cut(s) 742, 932, 972, 1018, 1300, 1600
BseJI GATNNNNATC 2 cut(s) 1094, 1467
BseLI CCNNNNNNNGG 6 cut(s) 73, 164, 361, 512, 770, 1060
BseMII CTCAG 4 cut(s) 633, 805, 1280, 1818
BseNI ACTGG 2 cut(s) 667, 816
BseRI GAGGAG 2 cut(s) 1019, 1952
BseXI GCAGC 8 cut(s) 814, 856, 859, 862, 871, 1108, 1412, 1615
BshFI GGCC 2 cut(s) 223, 902
BshNI GGYRCC 1 cut(s) 1418
BshVI ATCGAT 1 cut(s) 972
BsiSI CCGG 4 cut(s) 220, 293, 1148, 1632
BslFI GGGAC 2 cut(s) 244, 381
BslI CCNNNNNNNGG 6 cut(s) 73, 164, 361, 512, 770, 1060
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 2 cut(s) 244, 381
BsnI GGCC 2 cut(s) 223, 902
Bso31I GGTCTC 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 119
Bsp13I TCCGGA 1 cut(s) 292
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 2 cut(s) 374, 500
BspANI GGCC 2 cut(s) 223, 902
BspCNI CTCAG 4 cut(s) 632, 804, 1279, 1819
BspDI ATCGAT 1 cut(s) 972
BspEI TCCGGA 1 cut(s) 292
BspLI GGNNCC 7 cut(s) 35, 269, 504, 762, 1420, 1600, 1948
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 7 cut(s) 229, 303, 968, 1169, 1476, 1593, 1606
BspQI GCTCTTC 1 cut(s) 254
BspT104I TTCGAA 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 1418
BspTNI GGTCTC 1 cut(s) 294
BsrBI CCGCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 2 cut(s) 667, 816
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 5 cut(s) 29, 67, 1422, 1632, 1761
BssT1I CCWWGG 2 cut(s) 29, 1761
Bst2UI CCWGG 3 cut(s) 68, 413, 1679
Bst4CI ACNGT 4 cut(s) 188, 1113, 1232, 1798
Bst6I CTCTTC 5 cut(s) 254, 443, 996, 1860, 1897
BstAPI GCANNNNNTGC 2 cut(s) 208, 884
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 5 cut(s) 13, 200, 562, 867, 1512
BstDEI CTNAG 7 cut(s) 441, 619, 791, 1034, 1099, 1266, 1827
BstDSI CCRYGG 1 cut(s) 29
BstEII GGTNACC 1 cut(s) 1728
BstF5I GGATG 6 cut(s) 742, 932, 972, 1018, 1300, 1600
BstH2I RGCGCY 1 cut(s) 1629
BstHHI GCGC 1 cut(s) 1628
BstMAI GTCTC 1 cut(s) 294
BstMWI GCNNNNNNNGC 9 cut(s) 208, 833, 875, 881, 884, 893, 908, 1406, 1752
BstNI CCWGG 3 cut(s) 68, 413, 1679
BstPI GGTNACC 1 cut(s) 1728
BstSCI CCNGG 5 cut(s) 66, 411, 1147, 1631, 1677
BstSFI CTRYAG 1 cut(s) 1853
BstV1I GCAGC 8 cut(s) 814, 856, 859, 862, 871, 1108, 1412, 1615
BstX2I RGATCY 2 cut(s) 1468, 1598
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 2 cut(s) 1468, 1598
Bsu15I ATCGAT 1 cut(s) 972
BsuI GTATCC 1 cut(s) 1678
BsuRI GGCC 2 cut(s) 223, 902
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 29
BtgZI GCGATG 2 cut(s) 597, 911
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 6 cut(s) 742, 932, 972, 1018, 1300, 1600
BtsI GCAGTG 1 cut(s) 1651
BtsIMutI CAGTG 4 cut(s) 809, 1645, 1651, 1824
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 5 cut(s) 13, 200, 562, 867, 1512
CfoI GCGC 1 cut(s) 1628
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 972
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 7 cut(s) 958, 1233, 1260, 1334, 1358, 1419, 1671
CspCI CAANNNNNGTGG 2 cut(s) 1662, 1697
CviAII CATG 9 cut(s) 30, 106, 356, 904, 1313, 1363, 1487, 1529, 1901
CviQI GTAC 7 cut(s) 958, 1233, 1260, 1334, 1358, 1419, 1671
DdeI CTNAG 7 cut(s) 441, 619, 791, 1034, 1099, 1266, 1827
DraIII CACNNNGTG 1 cut(s) 1460
EaeI YGGCCR 1 cut(s) 900
Eam1104I CTCTTC 5 cut(s) 254, 443, 996, 1860, 1897
EarI CTCTTC 5 cut(s) 254, 443, 996, 1860, 1897
Eco130I CCWWGG 2 cut(s) 29, 1761
Eco31I GGTCTC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 33
Eco57I CTGAAG 2 cut(s) 1416, 1457
Eco91I GGTNACC 1 cut(s) 1728
EcoO65I GGTNACC 1 cut(s) 1728
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 3 cut(s) 66, 411, 1677
EcoT14I CCWWGG 2 cut(s) 29, 1761
EcoT22I ATGCAT 1 cut(s) 1722
ErhI CCWWGG 2 cut(s) 29, 1761
FaeI CATG 9 cut(s) 33, 109, 359, 907, 1316, 1366, 1490, 1532, 1904
FalI AAGNNNNNCTT 4 cut(s) 508, 540, 1014, 1046
FaqI GGGAC 2 cut(s) 244, 381
FatI CATG 9 cut(s) 29, 105, 355, 903, 1312, 1362, 1486, 1528, 1900
Fnu4HI GCNGC 9 cut(s) 501, 828, 870, 873, 876, 885, 1122, 1401, 1629
FokI GGATG 6 cut(s) 749, 919, 979, 1025, 1287, 1607
Fsp4HI GCNGC 9 cut(s) 501, 828, 870, 873, 876, 885, 1122, 1401, 1629
FspBI CTAG 5 cut(s) 381, 492, 848, 854, 1862
GlaI GCGC 1 cut(s) 1627
GluI GCNGC 9 cut(s) 501, 828, 870, 873, 876, 885, 1122, 1401, 1629
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 1629
HaeIII GGCC 2 cut(s) 223, 902
HapII CCGG 4 cut(s) 220, 293, 1148, 1632
HhaI GCGC 1 cut(s) 1628
Hin1II CATG 9 cut(s) 33, 109, 359, 907, 1316, 1366, 1490, 1532, 1904
Hin6I GCGC 1 cut(s) 1626
HinP1I GCGC 1 cut(s) 1626
HincII GTYRAC 1 cut(s) 1884
HindII GTYRAC 1 cut(s) 1884
HpaII CCGG 4 cut(s) 220, 293, 1148, 1632
HphI GGTGA 5 cut(s) 268, 380, 623, 1155, 1777
Hpy166II GTNNAC 6 cut(s) 247, 1145, 1334, 1360, 1794, 1884
Hpy188I TCNGA 9 cut(s) 329, 700, 982, 1020, 1117, 1342, 1476, 1897, 1946
Hpy188III TCNNGA 7 cut(s) 62, 293, 466, 506, 848, 1749, 1862
Hpy8I GTNNAC 6 cut(s) 247, 1145, 1334, 1360, 1794, 1884
HpyAV CCTTC 6 cut(s) 359, 510, 1060, 1170, 1176, 1559
HpyCH4III ACNGT 4 cut(s) 188, 1113, 1232, 1798
HpyCH4IV ACGT 1 cut(s) 732
HpyF10VI GCNNNNNNNGC 9 cut(s) 208, 833, 875, 881, 884, 893, 908, 1406, 1752
HpyF3I CTNAG 7 cut(s) 441, 619, 791, 1034, 1099, 1266, 1827
HpySE526I ACGT 1 cut(s) 732
Hsp92II CATG 9 cut(s) 33, 109, 359, 907, 1316, 1366, 1490, 1532, 1904
HspAI GCGC 1 cut(s) 1626
Kpn2I TCCGGA 1 cut(s) 292
KpnI GGTACC 1 cut(s) 1422
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 3 cut(s) 508, 766, 1751
Lsp1109I GCAGC 8 cut(s) 814, 856, 859, 862, 871, 1108, 1412, 1615
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 5 cut(s) 381, 492, 848, 854, 1862
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 2 cut(s) 743, 1728
MbiI CCGCTC 1 cut(s) 500
MflI RGATCY 2 cut(s) 1468, 1598
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 6 cut(s) 337, 480, 701, 986, 1480, 1835
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 4 cut(s) 345, 403, 454, 1766
MmeI TCCRAC 5 cut(s) 342, 751, 780, 1251, 1719
Mox20I TGGCCA 1 cut(s) 902
Mph1103I ATGCAT 1 cut(s) 1722
MroI TCCGGA 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 520
MscI TGGCCA 1 cut(s) 902
MslI CAYNNNNRTG 4 cut(s) 941, 1305, 1440, 1927
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 1514
MspI CCGG 4 cut(s) 220, 293, 1148, 1632
MspR9I CCNGG 5 cut(s) 68, 413, 1149, 1633, 1679
MvaI CCWGG 3 cut(s) 68, 413, 1679
MwoI GCNNNNNNNGC 9 cut(s) 208, 833, 875, 881, 884, 893, 908, 1406, 1752
NciI CCSGG 2 cut(s) 1149, 1633
NcoI CCATGG 1 cut(s) 29
NlaIII CATG 9 cut(s) 33, 109, 359, 907, 1316, 1366, 1490, 1532, 1904
NlaIV GGNNCC 7 cut(s) 35, 269, 504, 762, 1420, 1600, 1948
NsiI ATGCAT 1 cut(s) 1722
NspV TTCGAA 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 6 cut(s) 377, 1027, 1076, 1316, 1858, 1962
PflMI CCANNNNNTGG 2 cut(s) 361, 1060
PkrI GCNGC 9 cut(s) 502, 829, 871, 874, 877, 886, 1123, 1402, 1630
PleI GAGTC 4 cut(s) 345, 402, 454, 1766
PpsI GAGTC 4 cut(s) 345, 402, 454, 1766
Psp6I CCWGG 3 cut(s) 66, 411, 1677
PspEI GGTNACC 1 cut(s) 1728
PspGI CCWGG 3 cut(s) 66, 411, 1677
PspN4I GGNNCC 7 cut(s) 35, 269, 504, 762, 1420, 1600, 1948
PspPI GGNCC 1 cut(s) 33
PsuI RGATCY 2 cut(s) 1468, 1598
PvuII CAGCTG 1 cut(s) 1514
RsaI GTAC 7 cut(s) 959, 1234, 1261, 1335, 1359, 1420, 1672
RsaNI GTAC 7 cut(s) 958, 1233, 1260, 1334, 1358, 1419, 1671
RseI CAYNNNNRTG 4 cut(s) 941, 1305, 1440, 1927
SapI GCTCTTC 1 cut(s) 254
SatI GCNGC 9 cut(s) 501, 828, 870, 873, 876, 885, 1122, 1401, 1629
Sau96I GGNCC 1 cut(s) 33
ScaI AGTACT 1 cut(s) 1261
SchI GAGTC 4 cut(s) 345, 403, 454, 1766
ScrFI CCNGG 5 cut(s) 68, 413, 1149, 1633, 1679
SexAI ACCWGGT 1 cut(s) 411
SfcI CTRYAG 1 cut(s) 1853
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 4 cut(s) 941, 1305, 1440, 1927
SmlI CTYRAG 2 cut(s) 464, 1322
SmoI CTYRAG 2 cut(s) 464, 1322
Sse9I AATT 6 cut(s) 337, 480, 701, 986, 1480, 1835
SsiI CCGC 2 cut(s) 374, 500
SspI AATATT 1 cut(s) 1249
SspMI CTAG 5 cut(s) 381, 492, 848, 854, 1862
StyD4I CCNGG 5 cut(s) 66, 411, 1147, 1631, 1677
StyI CCWWGG 2 cut(s) 29, 1761
TaaI ACNGT 4 cut(s) 188, 1113, 1232, 1798
TaiI ACGT 1 cut(s) 735
TaqI TCGA 7 cut(s) 63, 119, 181, 392, 972, 1242, 1814
TasI AATT 6 cut(s) 337, 480, 701, 986, 1480, 1835
TatI WGTACW 4 cut(s) 1259, 1333, 1357, 1670
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 6 cut(s) 377, 1027, 1076, 1316, 1858, 1962
TscAI CASTG 4 cut(s) 816, 1645, 1651, 1831
TseI GCWGC 8 cut(s) 827, 869, 872, 875, 884, 1121, 1400, 1628
TspGWI ACGGA 2 cut(s) 375, 528
TspRI CASTG 4 cut(s) 816, 1645, 1651, 1831
Van91I CCANNNNNTGG 2 cut(s) 361, 1060
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 3 cut(s) 480, 986, 1480
XbaI TCTAGA 2 cut(s) 847, 1861
XcmI CCANNNNNNNNNTGG 1 cut(s) 1412
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 5 cut(s) 381, 492, 848, 854, 1862
ZrmI AGTACT 1 cut(s) 1261
Zsp2I ATGCAT 1 cut(s) 1722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.