pycom10g15300

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014662
Physical Location & Seq
Forward (+)
18572879 .. 18574609
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGAAATATAGCTGTGATGCTCAAGGACAACCCTATTTAGTCATCTCCTCGGATCCGGATGAGTCTTCTTCCGATGAAGATATCGATGAGAAATCTGATAATGGGTTCCTACAACTACAGGAGGCAAATAACATCTGCCTTGCAGCTTACAGAGAGGTTATGACATTGGCTATCAACGCAAAAGCTTGGAAGATGGGCCTTCCACCACTCGGTTCAATCGAGGATATACAACAAATGTTCAAGACTCTGCCTTCACCCGGAATGAAAGTAGAATCCTCCGTTGAGCGCGTCATTCTTCCTCTTTCTCCTAGGTTTTTGATAACTACTATGATTGACGTGGACAAATGGGCTTCAACCTTTTCCCACATTGTTCATCGAGGATGTGTACAAGACCCAACTGGTGTTGTTGGACGTCTATCGGCAATGCACAGAGGACAAGAATCTGGCAACCGTATCGATATGACAATTGTCAATGCTGAATTTCACTTGCCTACACCATTTGTGCAAAAACAAAAATACCGTTTTGTCAGATTTGTACGGAGCGTCATGGAAGATACATGGGCTGTAGTTGATCTATCAACTGATTATTTTCAGCAGCATTCACTTGATTCTGCTGCATCGAAAGTGAACTGTCTTCGGAGGCCATCTGGGGTCATTGTCAAGCGCCTCAGGACGTATGAAAATTTATCCGAGGTTATATGGATTGAAAATATGGAAGTGAAAGATTGCAAAGTTGATGATGATGTGGACGACGATGATATGCAGTCAGGAACAATGAATTCAAACCTAGCATTTGACGCAAAGCACTGGGTTAATATTTTGTCATCAAATCACAGAAGAAGATGTGCCAACCCTAAAATTTCATATATATCTGATCAAGTGACTCAAGGGGAGAAGAATGAACTGTCAATAGAAACCTTAATAAAAATTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

312

Amino Acids

35.23

Weight (kDa)

5.17

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 152 - 274 2.4e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 415
AccII CGCG 1 cut(s) 288
AccIII TCCGGA 1 cut(s) 55
AclWI GGATC 2 cut(s) 47, 60
AcsI RAATTY 4 cut(s) 479, 682, 778, 858
AcyI GRCGYC 1 cut(s) 412
AfaI GTAC 2 cut(s) 387, 537
AfiI CCNNNNNNNGG 2 cut(s) 209, 257
AgsI TTSAA 5 cut(s) 216, 241, 354, 707, 783
AjiI CACGTC 1 cut(s) 337
AluBI AGCT 3 cut(s) 12, 146, 185
AluI AGCT 3 cut(s) 12, 146, 185
AlwI GGATC 2 cut(s) 47, 60
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 2 cut(s) 196, 641
ApeKI GCWGC 3 cut(s) 143, 595, 614
ApoI RAATTY 4 cut(s) 479, 682, 778, 858
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 2 cut(s) 288, 666
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 246
AvrII CCTAGG 1 cut(s) 308
AxyI CCTNAGG 1 cut(s) 668
BamHI GGATCC 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 57, 626
BbvI GCAGC 3 cut(s) 155, 601, 607
BccI CCATC 2 cut(s) 187, 652
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 258
BfaI CTAG 2 cut(s) 309, 788
BfmI CTRYAG 2 cut(s) 116, 564
BfoI RGCGCY 1 cut(s) 667
BisI GCNGC 3 cut(s) 144, 596, 615
BlnI CCTAGG 1 cut(s) 308
BlsI GCNGC 3 cut(s) 145, 597, 616
Bme1390I CCNGG 1 cut(s) 258
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 54, 107
BmrFI CCNGG 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 817
BmsI GCATC 2 cut(s) 7, 626
BmuI ACTGGG 1 cut(s) 817
BoxI GACNNNNGTC 1 cut(s) 467
BpiI GAAGAC 2 cut(s) 57, 626
BpuEI CTTGAG 2 cut(s) 6, 870
BpuMI CCSGG 1 cut(s) 258
Bsa29I ATCGAT 2 cut(s) 84, 456
BsaHI GRCGYC 1 cut(s) 412
BsaJI CCNNGG 3 cut(s) 48, 308, 690
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 257
Bse1I ACTGG 2 cut(s) 403, 812
Bse21I CCTNAGG 1 cut(s) 668
Bse3DI GCAATG 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 55
BseCI ATCGAT 2 cut(s) 84, 456
BseDI CCNNGG 3 cut(s) 48, 308, 690
BseGI GGATG 2 cut(s) 64, 386
BseLI CCNNNNNNNGG 2 cut(s) 209, 257
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 682
BseNI ACTGG 2 cut(s) 403, 812
BseRI GAGGAG 1 cut(s) 37
BseXI GCAGC 3 cut(s) 155, 601, 607
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 198, 643
BshVI ATCGAT 2 cut(s) 84, 456
BsiSI CCGG 2 cut(s) 56, 258
BslI CCNNNNNNNGG 2 cut(s) 209, 257
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 2 cut(s) 198, 643
Bsp13I TCCGGA 1 cut(s) 55
Bsp1407I TGTACA 1 cut(s) 385
Bsp143I GATC 3 cut(s) 52, 571, 874
BspANI GGCC 2 cut(s) 198, 643
BspCNI CTCAG 1 cut(s) 681
BspDI ATCGAT 2 cut(s) 84, 456
BspEI TCCGGA 1 cut(s) 55
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 54, 107
BspPI GGATC 2 cut(s) 47, 60
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 385
BsrI ACTGG 2 cut(s) 403, 812
BssECI CCNNGG 3 cut(s) 48, 308, 690
BssMI GATC 3 cut(s) 52, 571, 874
BssNI GRCGYC 1 cut(s) 412
BssT1I CCWWGG 1 cut(s) 308
Bst4CI ACNGT 4 cut(s) 452, 521, 632, 906
BstACI GRCGYC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 668
BstF5I GGATG 2 cut(s) 64, 386
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 667
BstHHI GCGC 2 cut(s) 288, 666
BstKTI GATC 3 cut(s) 55, 574, 877
BstMBI GATC 3 cut(s) 52, 571, 874
BstMWI GCNNNNNNNGC 2 cut(s) 176, 797
BstPAI GACNNNNGTC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 2 cut(s) 116, 564
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 155, 601, 607
BstV2I GAAGAC 2 cut(s) 57, 626
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 2 cut(s) 84, 456
Bsu36I CCTNAGG 1 cut(s) 668
BsuRI GGCC 2 cut(s) 198, 643
BsuTUI ATCGAT 2 cut(s) 84, 456
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 2 cut(s) 64, 386
BtsIMutI CAGTG 1 cut(s) 805
CfoI GCGC 2 cut(s) 288, 666
Cfr13I GGNCC 1 cut(s) 196
ClaI ATCGAT 2 cut(s) 84, 456
CseI GACGC 3 cut(s) 277, 532, 806
Csp6I GTAC 2 cut(s) 386, 536
CviAII CATG 2 cut(s) 547, 558
CviJI RGCY 8 cut(s) 12, 146, 170, 185, 198, 350, 563, 643
CviKI_1 RGCY 8 cut(s) 12, 146, 170, 185, 198, 350, 563, 643
CviQI GTAC 2 cut(s) 386, 536
DdeI CTNAG 1 cut(s) 668
DpnI GATC 3 cut(s) 54, 573, 876
DpnII GATC 3 cut(s) 52, 571, 874
Eco130I CCWWGG 1 cut(s) 308
Eco32I GATATC 1 cut(s) 82
Eco81I CCTNAGG 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 778
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 2 cut(s) 550, 561
FatI CATG 2 cut(s) 546, 557
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 3 cut(s) 144, 596, 615
FokI GGATG 2 cut(s) 71, 393
Fsp4HI GCNGC 3 cut(s) 144, 596, 615
FspBI CTAG 2 cut(s) 309, 788
GlaI GCGC 2 cut(s) 287, 665
GluI GCNGC 3 cut(s) 144, 596, 615
HaeII RGCGCY 1 cut(s) 667
HaeIII GGCC 2 cut(s) 198, 643
HapII CCGG 2 cut(s) 56, 258
HgaI GACGC 3 cut(s) 277, 532, 806
HhaI GCGC 2 cut(s) 288, 666
Hin1I GRCGYC 1 cut(s) 412
Hin1II CATG 2 cut(s) 550, 561
Hin6I GCGC 2 cut(s) 286, 664
HinP1I GCGC 2 cut(s) 286, 664
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 6 cut(s) 62, 244, 272, 440, 608, 883
HpaII CCGG 2 cut(s) 56, 258
HphI GGTGA 1 cut(s) 246
Hpy166II GTNNAC 4 cut(s) 340, 386, 628, 748
Hpy188I TCNGA 7 cut(s) 52, 73, 97, 530, 639, 691, 874
Hpy188III TCNNGA 4 cut(s) 56, 241, 670, 768
Hpy8I GTNNAC 4 cut(s) 340, 386, 628, 748
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 2 cut(s) 209, 261
HpyCH4III ACNGT 4 cut(s) 452, 521, 632, 906
HpyCH4IV ACGT 3 cut(s) 336, 412, 674
HpyCH4V TGCA 6 cut(s) 143, 427, 505, 617, 729, 763
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 797
HpyF3I CTNAG 1 cut(s) 668
HpySE526I ACGT 3 cut(s) 336, 412, 674
Hsp92I GRCGYC 1 cut(s) 412
Hsp92II CATG 2 cut(s) 550, 561
HspAI GCGC 2 cut(s) 286, 664
Kpn2I TCCGGA 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 3 cut(s) 52, 571, 874
LmnI GCTCC 1 cut(s) 540
LpnPI CCDG 9 cut(s) 69, 104, 271, 384, 429, 633, 655, 753, 793
Lsp1109I GCAGC 3 cut(s) 155, 601, 607
LweI GCATC 2 cut(s) 7, 626
MaeI CTAG 2 cut(s) 309, 788
MaeII ACGT 3 cut(s) 336, 412, 674
MaeIII GTNAC 1 cut(s) 880
MalI GATC 3 cut(s) 54, 573, 876
MboI GATC 3 cut(s) 52, 571, 874
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 52
MluCI AATT 6 cut(s) 465, 479, 682, 778, 858, 927
MlyI GAGTC 3 cut(s) 71, 238, 877
MmeI TCCRAC 1 cut(s) 388
MroI TCCGGA 1 cut(s) 55
MseI TTAA 2 cut(s) 813, 920
MspI CCGG 2 cut(s) 56, 258
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 176, 797
NciI CCSGG 1 cut(s) 258
NdeII GATC 3 cut(s) 52, 571, 874
NlaIII CATG 2 cut(s) 550, 561
NlaIV GGNNCC 2 cut(s) 54, 107
NmuCI GTSAC 1 cut(s) 880
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 598
PfeI GAWTC 3 cut(s) 272, 440, 608
PkrI GCNGC 3 cut(s) 145, 597, 616
PleI GAGTC 3 cut(s) 70, 238, 877
PpsI GAGTC 3 cut(s) 70, 238, 877
PshAI GACNNNNGTC 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 54, 107
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 2 cut(s) 387, 537
RsaNI GTAC 2 cut(s) 386, 536
SaqAI TTAA 2 cut(s) 813, 920
SatI GCNGC 3 cut(s) 144, 596, 615
Sau3AI GATC 3 cut(s) 52, 571, 874
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 3 cut(s) 71, 238, 877
ScrFI CCNGG 1 cut(s) 258
SfaNI GCATC 2 cut(s) 7, 626
SfcI CTRYAG 2 cut(s) 116, 564
SmlI CTYRAG 2 cut(s) 21, 885
SmoI CTYRAG 2 cut(s) 21, 885
Sse9I AATT 6 cut(s) 465, 479, 682, 778, 858, 927
SspI AATATT 1 cut(s) 817
SspMI CTAG 2 cut(s) 309, 788
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 4 cut(s) 452, 521, 632, 906
TaiI ACGT 3 cut(s) 339, 415, 677
TaqI TCGA 5 cut(s) 84, 219, 376, 456, 620
TasI AATT 6 cut(s) 465, 479, 682, 778, 858, 927
TatI WGTACW 1 cut(s) 385
TfiI GAWTC 3 cut(s) 272, 440, 608
Tru1I TTAA 2 cut(s) 813, 920
Tru9I TTAA 2 cut(s) 813, 920
TscAI CASTG 1 cut(s) 812
TseFI GTSAC 1 cut(s) 880
TseI GCWGC 3 cut(s) 143, 595, 614
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 8 cut(s) 17, 90, 278, 362, 693, 791, 852, 915
TspGWI ACGGA 2 cut(s) 268, 553
TspRI CASTG 1 cut(s) 812
XapI RAATTY 4 cut(s) 479, 682, 778, 858
XmaJI CCTAGG 1 cut(s) 308
XspI CTAG 2 cut(s) 309, 788
ZraI GACGTC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.