pycom10g16660

Protein OSB2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012578
Physical Location & Seq
Reverse (-)
19833600 .. 19836223
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGGACACTAAGCCCATGACATTTCCATTTTTAGTTTGGGCCCGGTCCAAGAGGAGTTTGAGTTCATGCGTCCAAGTCTTAGTAGCATTATCCGGCTACGGCGGAGTTTGGCGCCTTTCCTCCCCCAAGAAACCACCGAGAGCAATGGCGTTGGAGCGAGCAATTCCGGCAGCAACAAGCGCAACCTTCCTCGCAAACCCCCAAGTTCCCACCTTTTTCCCATCCACCAACCACTTGGCCTTCGCACGGCGCCGTTTCAATCTCAAATGCTCGATGGAGTACAGTAGAGACCAGTACAACGGCAACGGGAGCAGCATTCAGGTGCCGATAGCGTACTCTAGACCGGCGGAGATTCAGTGGAAGAAGGAGCTGTGCAATACGGTGCACCTCATCGGCGTCGTCGGTCTTCCCGTCGAAATTAAGCACCTCCCCTCCGGAAAGGTCCTCGCTTGGACCCGCCTCGCCGTTAAGAAGTCCGCCACCGACACCTCTTGGATTAATCTGACATTTTGGGATGAGCTAGCACATGTTGCTTTTCAGCATGTAGAGAAAGGCCAACAAATATATGTAACGGGCCGTCTGATATCAGATTCTGTGGAAACCGATGAAGGGAAGCAGCAGACCTACTATAAGGTAGTTGTTCAGCAACTGAATTTTGTTGAAAGGGGCTTTTCATCGCTGGCTTTGAAAGATCACGACTCTGATTCATTTACAGCAGGCAAAAATCTTGGCAACAATGCTACAAAGAACATAACGGAAACCACACAAGAATTATGGCAAGCCTTCTTTGCTAATCCAGTTGAATGGTGGGATAATAGGAAGACCAAGAGGAACCCAAAATATCCCGATTTTAAGCATAAAGATACTGGAGAAGCCTTTTGGATTTAA

Protein Analysis

296

Amino Acids

33.46

Weight (kDa)

9.53

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 126 - 219 3.1e-17 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 111, 249, 322
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 4 cut(s) 102, 347, 457, 477
AcsI RAATTY 1 cut(s) 652
AcyI GRCGYC 3 cut(s) 112, 250, 396
AfaI GTAC 3 cut(s) 281, 296, 335
AfiI CCNNNNNNNGG 2 cut(s) 246, 609
AflIII ACRYGT 1 cut(s) 526
AgsI TTSAA 4 cut(s) 259, 662, 688, 803
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 2 cut(s) 370, 520
AluI AGCT 2 cut(s) 370, 520
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 282
Alw44I GTGCAC 1 cut(s) 383
AlwNI CAGNNNCTG 2 cut(s) 593, 649
Aor13HI TCCGGA 1 cut(s) 434
AoxI GGCC 4 cut(s) 39, 237, 553, 574
ApaI GGGCCC 1 cut(s) 43
ApaLI GTGCAC 1 cut(s) 383
ApeKI GCWGC 3 cut(s) 170, 312, 616
ApoI RAATTY 1 cut(s) 652
AseI ATTAAT 1 cut(s) 498
AspLEI GCGC 3 cut(s) 114, 182, 252
AspS9I GGNCC 6 cut(s) 39, 40, 45, 442, 453, 574
AsuC2I CCSGG 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 520
AvaII GGWCC 3 cut(s) 45, 442, 453
BaeGI GKGCMC 2 cut(s) 43, 387
BanI GGYRCC 3 cut(s) 111, 249, 322
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 398, 827
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 182, 324, 628
BccI CCATC 2 cut(s) 229, 268
BceAI ACGGC 6 cut(s) 115, 237, 263, 316, 449, 561
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 2 cut(s) 339, 521
BfoI RGCGCY 2 cut(s) 115, 253
BisI GCNGC 3 cut(s) 171, 313, 617
BlsI GCNGC 3 cut(s) 172, 314, 618
Bme1390I CCNGG 1 cut(s) 43
Bme18I GGWCC 3 cut(s) 45, 442, 453
BmgT120I GGNCC 6 cut(s) 39, 40, 45, 442, 453, 574
BmiI GGNNCC 6 cut(s) 41, 113, 251, 324, 455, 833
BmrFI CCNGG 1 cut(s) 43
BmtI GCTAGC 1 cut(s) 524
BpiI GAAGAC 2 cut(s) 398, 827
BpmI CTGGAG 1 cut(s) 888
BpuMI CCSGG 1 cut(s) 43
BsaHI GRCGYC 3 cut(s) 112, 250, 396
BsaI GGTCTC 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 341, 371, 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 609
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 3 cut(s) 292, 797, 871
Bse3DI GCAATG 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 434
BseGI GGATG 2 cut(s) 221, 520
BseLI CCNNNNNNNGG 2 cut(s) 246, 609
BseMI GCAATG 1 cut(s) 150
BseNI ACTGG 3 cut(s) 292, 797, 871
BseRI GAGGAG 1 cut(s) 67
BseSI GKGCMC 2 cut(s) 43, 387
BseXI GCAGC 3 cut(s) 182, 324, 628
BshFI GGCC 4 cut(s) 41, 239, 555, 576
BshNI GGYRCC 3 cut(s) 111, 249, 322
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 5 cut(s) 43, 93, 167, 344, 435
BslI CCNNNNNNNGG 2 cut(s) 246, 609
BsmAI GTCTC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 4 cut(s) 41, 239, 555, 576
Bso31I GGTCTC 1 cut(s) 282
Bsp120I GGGCCC 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 43, 387
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 1 cut(s) 691
BspACI CCGC 4 cut(s) 102, 347, 457, 477
BspANI GGCC 4 cut(s) 41, 239, 555, 576
BspEI TCCGGA 1 cut(s) 434
BspLI GGNNCC 6 cut(s) 41, 113, 251, 324, 455, 833
BspOI GCTAGC 1 cut(s) 524
BspT107I GGYRCC 3 cut(s) 111, 249, 322
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 3 cut(s) 292, 797, 871
BssAI RCCGGY 1 cut(s) 343
BssMI GATC 1 cut(s) 691
BssNI GRCGYC 3 cut(s) 112, 250, 396
Bst4CI ACNGT 2 cut(s) 284, 382
BstACI GRCGYC 3 cut(s) 112, 250, 396
BstAPI GCANNNNNTGC 1 cut(s) 530
BstC8I GCNNGC 5 cut(s) 159, 522, 681, 718, 780
BstDEI CTNAG 2 cut(s) 9, 79
BstF5I GGATG 2 cut(s) 221, 520
BstH2I RGCGCY 2 cut(s) 115, 253
BstHHI GCGC 3 cut(s) 114, 182, 252
BstKTI GATC 1 cut(s) 694
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 1 cut(s) 691
BstMWI GCNNNNNNNGC 5 cut(s) 167, 179, 309, 530, 788
BstNSI RCATGY 2 cut(s) 530, 545
BstSCI CCNGG 1 cut(s) 41
BstSLI GKGCMC 2 cut(s) 43, 387
BstV1I GCAGC 3 cut(s) 182, 324, 628
BstV2I GAAGAC 2 cut(s) 398, 827
BstXI CCANNNNNNTGG 2 cut(s) 235, 804
BsuRI GGCC 4 cut(s) 41, 239, 555, 576
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 2 cut(s) 221, 520
BtsIMutI CAGTG 1 cut(s) 362
Cac8I GCNNGC 5 cut(s) 159, 522, 681, 718, 780
CaiI CAGNNNCTG 2 cut(s) 593, 649
CfoI GCGC 3 cut(s) 114, 182, 252
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 6 cut(s) 39, 40, 45, 442, 453, 574
CseI GACGC 2 cut(s) 58, 385
Csp6I GTAC 3 cut(s) 280, 295, 334
CviAII CATG 4 cut(s) 16, 66, 527, 542
CviQI GTAC 3 cut(s) 280, 295, 334
DdeI CTNAG 2 cut(s) 9, 79
DinI GGCGCC 2 cut(s) 113, 251
DpnI GATC 1 cut(s) 693
DpnII GATC 1 cut(s) 691
EciI GGCGGA 3 cut(s) 117, 362, 466
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 585
Eco47I GGWCC 3 cut(s) 45, 442, 453
EcoO109I RGGNCCY 1 cut(s) 442
EcoRV GATATC 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 43
EgeI GGCGCC 2 cut(s) 113, 251
EheI GGCGCC 2 cut(s) 113, 251
FaeI CATG 4 cut(s) 19, 69, 530, 545
FatI CATG 4 cut(s) 15, 65, 526, 541
FauI CCCGC 1 cut(s) 464
Fnu4HI GCNGC 3 cut(s) 171, 313, 617
FokI GGATG 2 cut(s) 208, 527
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 3 cut(s) 171, 313, 617
FspBI CTAG 2 cut(s) 339, 521
GlaI GCGC 3 cut(s) 113, 181, 251
GluI GCNGC 3 cut(s) 171, 313, 617
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 2 cut(s) 115, 253
HaeIII GGCC 4 cut(s) 41, 239, 555, 576
HapII CCGG 5 cut(s) 43, 93, 167, 344, 435
HgaI GACGC 2 cut(s) 58, 385
HhaI GCGC 3 cut(s) 114, 182, 252
Hin1I GRCGYC 3 cut(s) 112, 250, 396
Hin1II CATG 4 cut(s) 19, 69, 530, 545
Hin6I GCGC 3 cut(s) 112, 180, 250
HinP1I GCGC 3 cut(s) 112, 180, 250
HinfI GANTC 4 cut(s) 352, 590, 698, 704
HpaII CCGG 5 cut(s) 43, 93, 167, 344, 435
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 4 cut(s) 504, 582, 589, 703
Hpy188III TCNNGA 4 cut(s) 339, 435, 695, 845
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 3 cut(s) 401, 404, 416
HpyAV CCTTC 5 cut(s) 196, 250, 358, 602, 793
HpyCH4III ACNGT 2 cut(s) 284, 382
HpyCH4V TGCA 2 cut(s) 375, 385
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 179, 309, 530, 788
HpyF3I CTNAG 2 cut(s) 9, 79
Hsp92I GRCGYC 3 cut(s) 112, 250, 396
Hsp92II CATG 4 cut(s) 19, 69, 530, 545
HspAI GCGC 3 cut(s) 112, 180, 250
KasI GGCGCC 2 cut(s) 111, 249
Kpn2I TCCGGA 1 cut(s) 434
Kzo9I GATC 1 cut(s) 691
LmnI GCTCC 3 cut(s) 154, 309, 367
Lsp1109I GCAGC 3 cut(s) 182, 324, 628
MaeI CTAG 2 cut(s) 339, 521
MaeIII GTNAC 1 cut(s) 568
MalI GATC 1 cut(s) 693
MboI GATC 1 cut(s) 691
MboII GAAGA 3 cut(s) 373, 398, 832
MhlI GDGCHC 2 cut(s) 43, 387
MluCI AATT 4 cut(s) 162, 417, 652, 770
Mly113I GGCGCC 2 cut(s) 112, 250
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 132
MroI TCCGGA 1 cut(s) 434
MseI TTAA 5 cut(s) 420, 468, 498, 852, 886
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 5 cut(s) 43, 93, 167, 344, 435
MspR9I CCNGG 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 315
MwoI GCNNNNNNNGC 5 cut(s) 167, 179, 309, 530, 788
NarI GGCGCC 2 cut(s) 112, 250
NciI CCSGG 1 cut(s) 43
NdeII GATC 1 cut(s) 691
NheI GCTAGC 1 cut(s) 520
NlaIII CATG 4 cut(s) 19, 69, 530, 545
NlaIV GGNNCC 6 cut(s) 41, 113, 251, 324, 455, 833
NspI RCATGY 2 cut(s) 530, 545
PciI ACATGT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 3 cut(s) 352, 590, 704
PkrI GCNGC 3 cut(s) 172, 314, 618
PleI GAGTC 1 cut(s) 692
PluTI GGCGCC 2 cut(s) 115, 253
PpsI GAGTC 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 442
PscI ACATGT 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 442
PspN4I GGNNCC 6 cut(s) 41, 113, 251, 324, 455, 833
PspOMI GGGCCC 1 cut(s) 39
PspPI GGNCC 6 cut(s) 39, 40, 45, 442, 453, 574
PspPPI RGGWCCY 1 cut(s) 442
PstNI CAGNNNCTG 2 cut(s) 593, 649
RsaI GTAC 3 cut(s) 281, 296, 335
RsaNI GTAC 3 cut(s) 280, 295, 334
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 5 cut(s) 420, 468, 498, 852, 886
SatI GCNGC 3 cut(s) 171, 313, 617
Sau3AI GATC 1 cut(s) 691
Sau96I GGNCC 6 cut(s) 39, 40, 45, 442, 453, 574
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 2 cut(s) 43, 387
SfoI GGCGCC 2 cut(s) 113, 251
SinI GGWCC 3 cut(s) 45, 442, 453
SmiMI CAYNNNNRTG 1 cut(s) 320
Sse9I AATT 4 cut(s) 162, 417, 652, 770
SsiI CCGC 4 cut(s) 102, 347, 457, 477
SspDI GGCGCC 2 cut(s) 111, 249
SspMI CTAG 2 cut(s) 339, 521
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 2 cut(s) 284, 382
TaqI TCGA 2 cut(s) 272, 414
TaqII GACCGA 1 cut(s) 392
TasI AATT 4 cut(s) 162, 417, 652, 770
TatI WGTACW 2 cut(s) 279, 294
TfiI GAWTC 3 cut(s) 352, 590, 704
Tru1I TTAA 5 cut(s) 420, 468, 498, 852, 886
Tru9I TTAA 5 cut(s) 420, 468, 498, 852, 886
TscAI CASTG 1 cut(s) 362
TseI GCWGC 3 cut(s) 170, 312, 616
TspDTI ATGAA 4 cut(s) 54, 621, 663, 696
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 1 cut(s) 362
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 3 cut(s) 45, 442, 453
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 1 cut(s) 652
XbaI TCTAGA 1 cut(s) 338
XceI RCATGY 2 cut(s) 530, 545
XcmI CCANNNNNNNNNTGG 3 cut(s) 33, 232, 804
XspI CTAG 2 cut(s) 339, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.