pycom10g20350

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011459
Physical Location & Seq
Forward (+)
23265292 .. 23268795
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGATCATGCCACGTCACAGAATCTTTCATCGTCATCGTCCAGATCATAGGTTTTCAAACTTTTTCATGCAGCATCACTCCATTTTATGCTCCCAGCTACTTTCAAAAGACTTCATCTTTGCCGCCGTGCGACGTTGTTTTGCCAAGGAACCGTTTAAAGCACCCAACAGCCGCCGAAACAGAACGGAAACTCGAGTGTCAATGGAGAAAGGAGGGTTAGACTCCGCCGGCCGAGAATTCAAGAACGCGGAGGAGATGTGGAGGGAGCAGGTCGGGGACGACCACAGCAAGAAGACCGAGTGGTACCGCCAAGGCGTTGGCTACTGGGAAGGTGTGGAGGCGTCGACCAATGGAGTGTTGGGAGGATATGCGCAGGTGAATGAGCCTGACATAATGGGCAGTGAAGCTTTTCTTAAGCAGCTTCTCTCTGAGCGTTTTCCCGACTCTGCAAATGGCCAACGTCACCTTGTTGTTCTTGATTGTGGTTCTGGCATTGGCAGAGTCTCCAAAAATCTTCTCATAAGATACTTCAATGAGGTTGATCTTCTTGAGCCTGTATCTCATTTCTTGGAAACTGCTCGTGAAAGTCTGGCTCCTGAAAATCATATGGTCTCTGAGACGCACAAAGCTACTAACTTTTTCTGCGTGCCTCTTCAGGAATTCACACCAGAAGCAGGGAGGTACGACATTATATGGGTTCAATGGTGTATTGGTCATCTGACAGATGATGACTTCGTCTCATTCTTTAAACGGGCAAAGGTTGGATTAAAACCTGGTGGTCTTTTTGTTCTGAAGGAAAATATTGCAAGATCCGGATTCGTGTTAGACACAGAAGATCGGAGTGTCACTAGGTCTGATTTGTACTTTAAGGAGCTCTTTCGTCAATGTGGACTTCATCTTTACATATCAAAGGATCAAAAGGGATTGCCTGAAGAACTATTCGCCGTGAAAATGTATGCATTAACTACTGAGTTGCCAAAGAAGGTTCATCGGACAAGATCTAAAGTGCAAGCCAACAGACCCGGAGTCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

346

Amino Acids

39.77

Weight (kDa)

8.92

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 98 - 322 2.1e-93 AdoMet dependent proline di-methyltransferase
Methyltransf_23 PF13489 140 - 266 4.2e-06 Methyltransferase domain
Methyltransf_25 PF13649 158 - 260 1.9e-09 Methyltransferase domain
Methyltransf_11 PF08241 159 - 264 1.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
Acc16I TGCGCA 1 cut(s) 372
Acc36I ACCTGC 2 cut(s) 259, 364
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 344
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 5 cut(s) 123, 172, 225, 248, 307
AclWI GGATC 2 cut(s) 804, 921
AcoI YGGCCR 2 cut(s) 229, 454
AcsI RAATTY 2 cut(s) 236, 659
AcuI CTGAAG 3 cut(s) 638, 812, 951
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 3 cut(s) 305, 683, 863
AfiI CCNNNNNNNGG 1 cut(s) 674
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 5 cut(s) 57, 105, 241, 532, 701
AhdI GACNNNNNGTC 1 cut(s) 1025
AjiI CACGTC 1 cut(s) 14
AjnI CCWGG 1 cut(s) 772
AluBI AGCT 5 cut(s) 97, 407, 421, 629, 874
AluI AGCT 5 cut(s) 97, 407, 421, 629, 874
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 4 cut(s) 508, 611, 616, 742
AlwI GGATC 2 cut(s) 804, 921
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 812
AoxI GGCC 2 cut(s) 229, 454
ApeKI GCWGC 2 cut(s) 70, 418
ApoI RAATTY 2 cut(s) 236, 659
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 373
AsuC2I CCSGG 1 cut(s) 1023
AsuHPI GGTGA 2 cut(s) 388, 455
AvaI CYCGRG 1 cut(s) 192
BalI TGGCCA 1 cut(s) 456
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 876
BauI CACGAG 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 2 cut(s) 82, 430
BceAI ACGGC 2 cut(s) 110, 929
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1023
BcoDI GTCTC 4 cut(s) 508, 611, 616, 742
BfaI CTAG 1 cut(s) 849
BfrI CTTAAG 1 cut(s) 413
BfuAI ACCTGC 2 cut(s) 259, 364
BglII AGATCT 1 cut(s) 998
BisI GCNGC 4 cut(s) 71, 123, 172, 419
BlsI GCNGC 4 cut(s) 72, 124, 173, 420
Bme1390I CCNGG 2 cut(s) 774, 1023
BmeRI GACNNNNNGTC 1 cut(s) 1025
BmeT110I CYCGRG 1 cut(s) 192
BmgBI CACGTC 1 cut(s) 14
BmiI GGNNCC 3 cut(s) 150, 305, 594
BmrFI CCNGG 2 cut(s) 774, 1023
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 82
BmuI ACTGGG 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 1023
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 2 cut(s) 144, 310
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 329
BseAI TCCGGA 1 cut(s) 812
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 2 cut(s) 144, 310
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMII CTCAG 3 cut(s) 420, 606, 960
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 266
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 82, 430
BseYI CCCAGC 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 231, 456
BshNI GGYRCC 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 876
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 3 cut(s) 228, 813, 1023
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 4 cut(s) 508, 611, 616, 742
BsmBI CGTCTC 2 cut(s) 611, 742
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 231, 456
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 876
Bsp13I TCCGGA 1 cut(s) 812
Bsp143I GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
BspACI CCGC 5 cut(s) 123, 172, 225, 248, 307
BspANI GGCC 2 cut(s) 231, 456
BspCNI CTCAG 3 cut(s) 421, 607, 961
BspEI TCCGGA 1 cut(s) 812
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 150, 305, 594
BspMI ACCTGC 2 cut(s) 259, 364
BspPI GGATC 2 cut(s) 804, 921
BspT107I GGYRCC 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 144, 310
BssMI GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
BssNI GRCGYC 1 cut(s) 341
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 2 cut(s) 144, 310
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 341
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 229, 647, 1011
BstDEI CTNAG 3 cut(s) 429, 615, 969
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 7 cut(s) 6, 46, 544, 812, 838, 916, 1001
BstMAI GTCTC 4 cut(s) 508, 611, 616, 742
BstMBI GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 1 cut(s) 774
BstSCI CCNGG 2 cut(s) 772, 1021
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 82, 430
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 2 cut(s) 809, 998
BstXI CCANNNNNNTGG 1 cut(s) 317
BstYI RGATCY 2 cut(s) 809, 998
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 2 cut(s) 231, 456
BtrI CACGTC 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
BveI ACCTGC 2 cut(s) 259, 364
Cac8I GCNNGC 3 cut(s) 229, 647, 1011
CfoI GCGC 1 cut(s) 373
Cfr10I RCCGGY 1 cut(s) 227
CseI GACGC 2 cut(s) 330, 628
CsiI ACCWGGT 1 cut(s) 772
Csp6I GTAC 3 cut(s) 304, 682, 862
CviAII CATG 2 cut(s) 7, 67
CviQI GTAC 3 cut(s) 304, 682, 862
DdeI CTNAG 3 cut(s) 429, 615, 969
DpnI GATC 7 cut(s) 5, 45, 543, 811, 837, 915, 1000
DpnII GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
DraI TTTAAA 2 cut(s) 157, 748
DriI GACNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 2 cut(s) 229, 454
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 657
Eam1105I GACNNNNNGTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 874
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 144, 310
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 616
Eco52I CGGCCG 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 3 cut(s) 638, 812, 951
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 874
EcoRI GAATTC 2 cut(s) 236, 659
EcoRII CCWGG 1 cut(s) 772
EcoT14I CCWWGG 2 cut(s) 144, 310
EcoT22I ATGCAT 1 cut(s) 961
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 2 cut(s) 144, 310
Esp3I CGTCTC 2 cut(s) 611, 742
FaeI CATG 2 cut(s) 10, 70
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 860, 892
FaqI GGGAC 1 cut(s) 290
FatI CATG 2 cut(s) 6, 66
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 71, 123, 172, 419
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 4 cut(s) 71, 123, 172, 419
FspBI CTAG 1 cut(s) 849
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 1 cut(s) 372
GluI GCNGC 4 cut(s) 71, 123, 172, 419
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 2 cut(s) 231, 456
HapII CCGG 3 cut(s) 228, 813, 1023
HgaI GACGC 2 cut(s) 330, 628
HhaI GCGC 1 cut(s) 373
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 2 cut(s) 10, 70
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 6 cut(s) 21, 221, 443, 501, 816, 1026
HpaII CCGG 3 cut(s) 228, 813, 1023
HphI GGTGA 2 cut(s) 388, 455
Hpy166II GTNNAC 2 cut(s) 345, 890
Hpy188I TCNGA 7 cut(s) 430, 616, 720, 792, 840, 856, 993
Hpy188III TCNNGA 9 cut(s) 41, 241, 440, 476, 548, 581, 596, 656, 813
Hpy8I GTNNAC 2 cut(s) 345, 890
Hpy99I CGWCG 2 cut(s) 135, 346
HpyAV CCTTC 3 cut(s) 323, 787, 976
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4IV ACGT 3 cut(s) 13, 133, 460
HpyCH4V TGCA 5 cut(s) 70, 449, 806, 959, 1009
HpyF3I CTNAG 3 cut(s) 429, 615, 969
HpySE526I ACGT 3 cut(s) 13, 133, 460
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 2 cut(s) 10, 70
HspAI GCGC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 812
KpnI GGTACC 1 cut(s) 307
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
LmnI GCTCC 4 cut(s) 95, 265, 598, 871
Lsp1109I GCAGC 2 cut(s) 82, 430
LweI GCATC 1 cut(s) 82
MabI ACCWGGT 1 cut(s) 772
MaeI CTAG 1 cut(s) 849
MaeII ACGT 3 cut(s) 13, 133, 460
MaeIII GTNAC 3 cut(s) 14, 461, 844
MalI GATC 7 cut(s) 5, 45, 543, 811, 837, 915, 1000
MboI GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
MboII GAAGA 6 cut(s) 304, 506, 536, 644, 845, 944
MflI RGATCY 2 cut(s) 809, 998
MhlI GDGCHC 1 cut(s) 876
MlsI TGGCCA 1 cut(s) 456
MluCI AATT 2 cut(s) 236, 659
MluNI TGGCCA 1 cut(s) 456
MlyI GAGTC 4 cut(s) 215, 437, 510, 1035
MmeI TCCRAC 1 cut(s) 742
MnlI CCTC 8 cut(s) 206, 244, 255, 331, 356, 529, 660, 672
Mox20I TGGCCA 1 cut(s) 456
Mph1103I ATGCAT 1 cut(s) 961
MroI TCCGGA 1 cut(s) 812
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 456
MseI TTAA 6 cut(s) 156, 414, 747, 767, 867, 962
Msp20I TGGCCA 1 cut(s) 456
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 3 cut(s) 228, 813, 1023
MspR9I CCNGG 2 cut(s) 774, 1023
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 1 cut(s) 248
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 1023
NdeI CATATG 1 cut(s) 606
NdeII GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
NgoMIV GCCGGC 1 cut(s) 227
NlaIII CATG 2 cut(s) 10, 70
NlaIV GGNNCC 3 cut(s) 150, 305, 594
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 3 cut(s) 14, 461, 844
NsbI TGCGCA 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 961
PaeR7I CTCGAG 1 cut(s) 192
PaqCI CACCTGC 1 cut(s) 364
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 21, 816
PflFI GACNNNGTC 1 cut(s) 734
PkrI GCNGC 4 cut(s) 72, 124, 173, 420
PleI GAGTC 4 cut(s) 215, 437, 509, 1034
PpsI GAGTC 4 cut(s) 215, 437, 509, 1034
Psp124BI GAGCTC 1 cut(s) 876
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 150, 305, 594
PspXI VCTCGAGB 1 cut(s) 192
PsuI RGATCY 2 cut(s) 809, 998
PsyI GACNNNGTC 1 cut(s) 734
RsaI GTAC 3 cut(s) 305, 683, 863
RsaNI GTAC 3 cut(s) 304, 682, 862
SacI GAGCTC 1 cut(s) 876
SalI GTCGAC 1 cut(s) 343
SaqAI TTAA 6 cut(s) 156, 414, 747, 767, 867, 962
SatI GCNGC 4 cut(s) 71, 123, 172, 419
Sau3AI GATC 7 cut(s) 3, 43, 541, 809, 835, 913, 998
SchI GAGTC 4 cut(s) 215, 437, 510, 1035
ScrFI CCNGG 2 cut(s) 774, 1023
SduI GDGCHC 1 cut(s) 876
SexAI ACCWGGT 1 cut(s) 772
SfaNI GCATC 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 3 cut(s) 192, 413, 548
SmoI CTYRAG 3 cut(s) 192, 413, 548
Sse9I AATT 2 cut(s) 236, 659
SsiI CCGC 5 cut(s) 123, 172, 225, 248, 307
SspI AATATT 1 cut(s) 802
SspMI CTAG 1 cut(s) 849
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 2 cut(s) 772, 1021
StyI CCWWGG 2 cut(s) 144, 310
TaaI ACNGT 1 cut(s) 153
TaiI ACGT 3 cut(s) 16, 136, 463
TaqI TCGA 2 cut(s) 193, 344
TaqII GACCGA 1 cut(s) 311
TasI AATT 2 cut(s) 236, 659
TatI WGTACW 1 cut(s) 861
TauI GCSGC 2 cut(s) 125, 174
TfiI GAWTC 2 cut(s) 21, 816
Tru1I TTAA 6 cut(s) 156, 414, 747, 767, 867, 962
Tru9I TTAA 6 cut(s) 156, 414, 747, 767, 867, 962
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 3 cut(s) 14, 461, 844
TseI GCWGC 2 cut(s) 70, 418
Tsp45I GTSAC 3 cut(s) 14, 461, 844
TspDTI ATGAA 5 cut(s) 17, 55, 103, 884, 977
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 1 cut(s) 406
Tth111I GACNNNGTC 1 cut(s) 734
Vha464I CTTAAG 1 cut(s) 413
XapI RAATTY 2 cut(s) 236, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 192
XmiI GTMKAC 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 1 cut(s) 849
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.