pycom10g20570

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014835
Physical Location & Seq
Forward (+)
23458094 .. 23459421
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGTTTTCTCCCATTTTGATAGTGTCTGATACGGTTGTGGAACCATACAATGCCACCCTTTCCGTCCATCAGCTTGTCGAAAATGCTGATGAATTTGGGGATCTTAATCATCTCATCTCTGCTACCATGAGCGGTGTCACCTGTTGTCTTCGGTTCCCTGGACAGTTGAACTCTGACCTACGGAAGCTTGCAGTTAACCTTATCCCATTCCCGCGGTTGCACTTCTTTATGGTTGGGTTTGCACCCCTAACTTCAAGAGGATCACAGCAGTACCGCGCTCTGTCTGTGCCCGACTTGACCCAGCAGATGTGGGATGCCAAAAACATGATGTGTGCCGCTGACCCACGCCATGGACGTTACCTTACTGCGTCTGCCATGTTCCATGGTAAGATGAGCACCAAAGAAGTTGATGAACAGATGATTAACGTCCAGAACAAAAACTCCTCATATTTTGTTGAGTGGATTCCCAATAACGTTAAGTCCAGCGTGTGTGACATCCCACCCAAGGGTCTAAAGATGGCATCCACTTTTATTGGGAATTCGACTTCAATTCAGGAAATGTTCAGGAGAGTTAGCGAGCAGTTCACTGCTATGTTCAGGCGAAAGGCTTTCTTGCATTGGTACACAGGTGAAGGAATGGATGAGATGGAGTTCACCGAGGCCGAGAGTAACATGAACGATCTAGTTGCTGAGTACCAACAATACGAGGATGCAATTATCGAAGAGGAGTACGAAGAGGAGGAAGAGGAAGAAAAGGAGGTTGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

257

Amino Acids

29.22

Weight (kDa)

4.72

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 71 - 192 1.9e-40 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 132
AccII CGCG 2 cut(s) 214, 276
AciI CCGC 5 cut(s) 132, 212, 214, 274, 336
AclI AACGTT 1 cut(s) 474
AclWI GGATC 2 cut(s) 108, 268
AcsI RAATTY 2 cut(s) 92, 538
AfaI GTAC 4 cut(s) 272, 623, 695, 731
AfiI CCNNNNNNNGG 3 cut(s) 350, 505, 506
AgsI TTSAA 3 cut(s) 169, 255, 549
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 1 cut(s) 744
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 2 cut(s) 73, 187
AluI AGCT 2 cut(s) 73, 187
Alw21I GWGCWC 1 cut(s) 398
AlwI GGATC 2 cut(s) 108, 268
AoxI GGCC 1 cut(s) 660
ApoI RAATTY 2 cut(s) 92, 538
AspLEI GCGC 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 130, 641, 646
BaeGI GKGCMC 1 cut(s) 291
BaeI ACNNNNGTAYC 6 cut(s) 21, 54, 254, 287, 613, 646
BbsI GAAGAC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 398
BccI CCATC 3 cut(s) 75, 511, 640
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 1 cut(s) 159
BfaI CTAG 1 cut(s) 683
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 159
BmiI GGNNCC 2 cut(s) 42, 155
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 304, 530, 700
BpiI GAAGAC 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 157, 212, 349, 382, 504, 657
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 505, 506
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 6 cut(s) 157, 212, 349, 382, 504, 657
BseGI GGATG 5 cut(s) 319, 495, 521, 646, 715
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 3 cut(s) 350, 505, 506
BseMII CTCAG 1 cut(s) 681
BseRI GAGGAG 3 cut(s) 433, 740, 752
BseSI GKGCMC 1 cut(s) 291
BseYI CCCAGC 1 cut(s) 300
Bsh1236I CGCG 2 cut(s) 214, 276
BshFI GGCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 398
BslI CCNNNNNNNGG 3 cut(s) 350, 505, 506
BsnI GGCC 1 cut(s) 662
Bsp1286I GDGCHC 2 cut(s) 291, 398
Bsp143I GATC 3 cut(s) 100, 260, 679
Bsp19I CCATGG 2 cut(s) 349, 382
BspACI CCGC 5 cut(s) 132, 212, 214, 274, 336
BspANI GGCC 1 cut(s) 662
BspCNI CTCAG 1 cut(s) 682
BspFNI CGCG 2 cut(s) 214, 276
BspLI GGNNCC 2 cut(s) 42, 155
BspPI GGATC 2 cut(s) 108, 268
BsrBI CCGCTC 1 cut(s) 132
BssECI CCNNGG 6 cut(s) 157, 212, 349, 382, 504, 657
BssMI GATC 3 cut(s) 100, 260, 679
BssT1I CCWWGG 3 cut(s) 349, 382, 504
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 34, 165
Bst6I CTCTTC 3 cut(s) 717, 729, 738
BstC8I GCNNGC 2 cut(s) 189, 578
BstDEI CTNAG 1 cut(s) 690
BstDSI CCRYGG 3 cut(s) 212, 349, 382
BstF5I GGATG 5 cut(s) 319, 495, 521, 646, 715
BstFNI CGCG 2 cut(s) 214, 276
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 3 cut(s) 103, 263, 682
BstMBI GATC 3 cut(s) 100, 260, 679
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 2 cut(s) 214, 276
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 1 cut(s) 662
BtgI CCRYGG 3 cut(s) 212, 349, 382
BtsCI GGATG 5 cut(s) 319, 495, 521, 646, 715
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 2 cut(s) 189, 578
CfoI GCGC 1 cut(s) 278
Cfr42I CCGCGG 1 cut(s) 215
CseI GACGC 1 cut(s) 357
Csp6I GTAC 4 cut(s) 271, 622, 694, 730
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 6 cut(s) 127, 325, 350, 376, 383, 673
CviJI RGCY 4 cut(s) 73, 187, 608, 662
CviKI_1 RGCY 4 cut(s) 73, 187, 608, 662
CviQI GTAC 4 cut(s) 271, 622, 694, 730
DdeI CTNAG 1 cut(s) 690
DpnI GATC 3 cut(s) 102, 262, 681
DpnII GATC 3 cut(s) 100, 260, 679
Eam1104I CTCTTC 3 cut(s) 717, 729, 738
EarI CTCTTC 3 cut(s) 717, 729, 738
Eco130I CCWWGG 3 cut(s) 349, 382, 504
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 3 cut(s) 349, 382, 504
ErhI CCWWGG 3 cut(s) 349, 382, 504
FaeI CATG 6 cut(s) 130, 328, 353, 379, 386, 676
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 6 cut(s) 126, 324, 349, 375, 382, 672
FauI CCCGC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 5 cut(s) 326, 482, 508, 653, 722
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 277
GluI GCNGC 1 cut(s) 336
GsaI CCCAGC 1 cut(s) 304
HaeIII GGCC 1 cut(s) 662
HgaI GACGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 278
Hin1II CATG 6 cut(s) 130, 328, 353, 379, 386, 676
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 1 cut(s) 463
HpaI GTTAAC 1 cut(s) 196
HphI GGTGA 3 cut(s) 130, 641, 646
Hpy166II GTNNAC 4 cut(s) 196, 585, 624, 654
Hpy188I TCNGA 2 cut(s) 28, 175
Hpy188III TCNNGA 4 cut(s) 255, 430, 554, 565
Hpy8I GTNNAC 4 cut(s) 196, 585, 624, 654
HpyAV CCTTC 1 cut(s) 626
HpyCH4III ACNGT 2 cut(s) 34, 165
HpyCH4IV ACGT 3 cut(s) 355, 426, 474
HpyCH4V TGCA 5 cut(s) 191, 220, 242, 616, 713
HpyF3I CTNAG 1 cut(s) 690
HpySE526I ACGT 3 cut(s) 355, 426, 474
Hsp92II CATG 6 cut(s) 130, 328, 353, 379, 386, 676
HspAI GCGC 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 215
Kzo9I GATC 3 cut(s) 100, 260, 679
LweI GCATC 3 cut(s) 304, 530, 700
MaeI CTAG 1 cut(s) 683
MaeII ACGT 3 cut(s) 355, 426, 474
MaeIII GTNAC 4 cut(s) 136, 356, 491, 668
MalI GATC 3 cut(s) 102, 262, 681
MbiI CCGCTC 1 cut(s) 132
MboI GATC 3 cut(s) 100, 260, 679
MboII GAAGA 5 cut(s) 140, 734, 746, 755, 761
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 291, 398
MluCI AATT 4 cut(s) 92, 538, 549, 714
MnlI CCTC 9 cut(s) 251, 454, 652, 700, 718, 730, 733, 739, 751
MseI TTAA 4 cut(s) 105, 195, 423, 477
MslI CAYNNNNRTG 1 cut(s) 590
MspA1I CMGCKG 2 cut(s) 214, 338
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 2 cut(s) 214, 276
NcoI CCATGG 2 cut(s) 349, 382
NdeII GATC 3 cut(s) 100, 260, 679
NlaIII CATG 6 cut(s) 130, 328, 353, 379, 386, 676
NlaIV GGNNCC 2 cut(s) 42, 155
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 2 cut(s) 136, 491
PcsI WCGNNNNNNNCGW 1 cut(s) 352
PfeI GAWTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 1 cut(s) 337
Psp1406I AACGTT 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 42, 155
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 4 cut(s) 272, 623, 695, 731
RsaNI GTAC 4 cut(s) 271, 622, 694, 730
RseI CAYNNNNRTG 1 cut(s) 590
SacII CCGCGG 1 cut(s) 215
SaqAI TTAA 4 cut(s) 105, 195, 423, 477
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 100, 260, 679
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 2 cut(s) 291, 398
SfaNI GCATC 3 cut(s) 304, 530, 700
Sfr303I CCGCGG 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 590
Sse9I AATT 4 cut(s) 92, 538, 549, 714
SsiI CCGC 5 cut(s) 132, 212, 214, 274, 336
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 3 cut(s) 349, 382, 504
TaaI ACNGT 2 cut(s) 34, 165
TaiI ACGT 3 cut(s) 358, 429, 477
TaqI TCGA 3 cut(s) 78, 542, 720
TasI AATT 4 cut(s) 92, 538, 549, 714
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 4 cut(s) 105, 195, 423, 477
Tru9I TTAA 4 cut(s) 105, 195, 423, 477
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 2 cut(s) 136, 491
Tsp45I GTSAC 2 cut(s) 136, 491
TspDTI ATGAA 3 cut(s) 105, 426, 689
TspGWI ACGGA 2 cut(s) 52, 196
TspRI CASTG 1 cut(s) 592
Van91I CCANNNNNTGG 1 cut(s) 350
XapI RAATTY 2 cut(s) 92, 538
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.