Rh7AG016100

structural constituent of cytoskeleton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014835
Physical Location & Seq
Reverse (-)
1061796 .. 1062263
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG016100.1

Sequence Viewer

Length: 468 bp
ATGTGGGATGCCAAGAACATGATGTGTGCAGCTGATCCACGCCACGGCCGTTACCTCACAGCATCAGCCATGTTTAGAGGCAAAATGAGCACAAAAGAAGTGGACGAGCAGATGATGAATGTGCAGAACAAGAACTCCTCCTACTTTGTTGAGTGGATCCCCAACAATGTCAAGTCCAGTGTTTGTGACATCCCGCCTAAGGGTCTCACCATGTCTTCAACTTTCATTGGGAATTCCACTTCCATACAAGAGATGTTCCGAAGAGTAAGCGAACAGTTCACAGCCATGTTTAGGAGGAAGGCCTTCTTGCACTGGTATACGGGAGAAGGAATGGACGAGATGGAATTCACTGAAGCCGAGAGTAACATGAATGATCTCGTTGCTGAATATCAACAGTACCAAGATGCTACGGTTGAAGAAGATGTTGAGTATGAAGAAGAAGAAGTGATTGGCGGTGTTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

155

Amino Acids

17.94

Weight (kDa)

4.51

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 2 - 90 2e-27 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 317
AciI CCGC 2 cut(s) 194, 453
AclWI GGATC 3 cut(s) 29, 151, 164
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 232, 344
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 4 cut(s) 44, 199, 200, 291
AgsI TTSAA 3 cut(s) 219, 416, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 3 cut(s) 29, 151, 164
AoxI GGCC 2 cut(s) 46, 300
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 232, 344
Asp700I GAANNNNTTC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 199
AxyI CCTNAGG 1 cut(s) 198
BamHI GGATCC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 334
BceAI ACGGC 2 cut(s) 33, 61
BcoDI GTCTC 1 cut(s) 209
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 158
BmsI GCATC 2 cut(s) 71, 394
BpiI GAAGAC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 199, 200, 291
Bse1I ACTGG 2 cut(s) 177, 317
Bse21I CCTNAGG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 2 cut(s) 13, 189
BseLI CCNNNNNNNGG 4 cut(s) 44, 199, 200, 291
BseNI ACTGG 2 cut(s) 177, 317
BseRI GAGGAG 1 cut(s) 127
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 2 cut(s) 48, 143
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 2 cut(s) 48, 302
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 44, 199, 200, 291
BsmAI GTCTC 1 cut(s) 209
BsnI GGCC 2 cut(s) 48, 302
Bso31I GGTCTC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 3 cut(s) 34, 156, 373
BspACI CCGC 2 cut(s) 194, 453
BspANI GGCC 2 cut(s) 48, 302
BspLI GGNNCC 1 cut(s) 158
BspPI GGATC 3 cut(s) 29, 151, 164
BspTNI GGTCTC 1 cut(s) 209
BsrI ACTGG 2 cut(s) 177, 317
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 34, 156, 373
BssNAI GTATAC 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 276, 396, 412
Bst6I CTCTTC 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 13, 189
BstKTI GATC 3 cut(s) 37, 159, 376
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 3 cut(s) 34, 156, 373
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BstZ17I GTATAC 1 cut(s) 318
BstZI CGGCCG 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 2 cut(s) 48, 302
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 13, 189
BtsIMutI CAGTG 3 cut(s) 184, 310, 348
Csp6I GTAC 1 cut(s) 397
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviAII CATG 5 cut(s) 19, 70, 211, 286, 367
CviJI RGCY 6 cut(s) 32, 48, 68, 284, 302, 356
CviKI_1 RGCY 6 cut(s) 32, 48, 68, 284, 302, 356
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 1 cut(s) 198
DpnI GATC 3 cut(s) 36, 158, 375
DpnII GATC 3 cut(s) 34, 156, 373
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 256
EclXI CGGCCG 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 302
Eco31I GGTCTC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 372
Eco81I CCTNAGG 1 cut(s) 198
EcoRI GAATTC 2 cut(s) 232, 344
FaeI CATG 5 cut(s) 22, 73, 214, 289, 370
FaiI YATR 8 cut(s) 20, 71, 212, 245, 287, 318, 368, 432
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 5 cut(s) 18, 69, 210, 285, 366
FauI CCCGC 1 cut(s) 201
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 20, 176
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 48, 302
Hin1II CATG 5 cut(s) 22, 73, 214, 289, 370
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 3 cut(s) 103, 279, 318
Hpy188I TCNGA 1 cut(s) 260
Hpy8I GTNNAC 3 cut(s) 103, 279, 318
HpyAV CCTTC 3 cut(s) 292, 313, 320
HpyCH4III ACNGT 3 cut(s) 276, 396, 412
HpyCH4V TGCA 3 cut(s) 29, 124, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 5 cut(s) 22, 73, 214, 289, 370
Kzo9I GATC 3 cut(s) 34, 156, 373
LpnPI CCDG 2 cut(s) 190, 298
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 71, 394
MaeIII GTNAC 3 cut(s) 50, 185, 362
MalI GATC 3 cut(s) 36, 158, 375
MboI GATC 3 cut(s) 34, 156, 373
MboII GAAGA 7 cut(s) 207, 273, 428, 431, 446, 449, 452
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 2 cut(s) 232, 344
MnlI CCTC 4 cut(s) 65, 71, 148, 288
MroXI GAANNNNTTC 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 87
NdeII GATC 3 cut(s) 34, 156, 373
NlaIII CATG 5 cut(s) 22, 73, 214, 289, 370
NlaIV GGNNCC 1 cut(s) 158
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 185
PceI AGGCCT 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 302
PkrI GCNGC 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 158
PsrI GAACNNNNNNTAC 2 cut(s) 125, 157
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 284
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 3 cut(s) 34, 156, 373
SduI GDGCHC 1 cut(s) 92
SetI ASST 2 cut(s) 34, 57
SfaNI GCATC 2 cut(s) 71, 394
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 2 cut(s) 232, 344
SseBI AGGCCT 1 cut(s) 302
SsiI CCGC 2 cut(s) 194, 453
StuI AGGCCT 1 cut(s) 302
TaaI ACNGT 3 cut(s) 276, 396, 412
TasI AATT 2 cut(s) 232, 344
TscAI CASTG 3 cut(s) 184, 317, 355
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 4 cut(s) 131, 214, 383, 447
TspRI CASTG 3 cut(s) 184, 317, 355
XapI RAATTY 2 cut(s) 232, 344
XmiI GTMKAC 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.