Rmu_ssc0000354.1_g000001

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000354.1
OG0014835
Physical Location & Seq
Reverse (-)
933 .. 1577
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000354.1_g000001.1.cds

Sequence Viewer

Length: 645 bp
atgtctggagttacatgctgcctaaggttccctggacagcttaactctgatttgcgcaagcttgctgttaacctcatccccttccctcgtcttcactttttcatggtcggttttgctcctctgacctcaagagggtctcagcagtacagagctttgactgtcccagaactcacccagcaaatgtgggattccaagaacatgatgtgtgctgctgatccccgacatggaaggtacctcacagcatcagccatgttccgtggtaagatgagcaccaaggaagtggatgaccagatgatgaatgtgcagaacaagaactcttcctactttgttgagtggatccccaacaacgtcaagtcaacagtctgtgacatcccaccaactggtctcaagatggcttcaactttcatcggaaactctacatccattcaagagatgttcaggagggtgagtgagcagttcactgctatgttcaggaggaaggctttcttgcattggtacactggtgaaggcatggacgagatggaattcactgaagctgagagcaacatgaatgatctggtgtcagaatatcagcagtaccaggatgccaccgctgatgaggacgagtatgaagatgaacagggagagtatgaggaggaaatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

214

Amino Acids

24.73

Weight (kDa)

4.77

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 56
Acc65I GGTACC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 231
AccB7I CCANNNNNTGG 1 cut(s) 380
AciI CCGC 1 cut(s) 591
AclWI GGATC 3 cut(s) 209, 331, 344
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 4 cut(s) 146, 233, 497, 578
AfiI CCNNNNNNNGG 3 cut(s) 132, 224, 380
AgsI TTSAA 2 cut(s) 399, 428
AjnI CCWGG 2 cut(s) 31, 579
AluBI AGCT 4 cut(s) 40, 61, 152, 536
AluI AGCT 4 cut(s) 40, 61, 152, 536
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 2 cut(s) 141, 389
AlwI GGATC 3 cut(s) 209, 331, 344
ApeKI GCWGC 2 cut(s) 18, 209
ApoI RAATTY 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 482
Asp718I GGTACC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 3 cut(s) 163, 457, 515
AxyI CCTNAGG 1 cut(s) 23
BaeI ACNNNNGTAYC 2 cut(s) 487, 520
BamHI GGATCC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 5, 196
BccI CCATC 2 cut(s) 385, 514
BciT130I CCWGG 2 cut(s) 33, 581
BcoDI GTCTC 2 cut(s) 141, 389
BisI GCNGC 2 cut(s) 19, 210
BlsI GCNGC 2 cut(s) 20, 211
Bme1390I CCNGG 2 cut(s) 33, 581
BmiI GGNNCC 3 cut(s) 29, 233, 338
BmrFI CCNGG 2 cut(s) 33, 581
BmsI GCATC 2 cut(s) 251, 574
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 2 cut(s) 112, 371
BsaI GGTCTC 2 cut(s) 141, 389
BsaJI CCNNGG 3 cut(s) 31, 256, 273
BsaXI ACNNNNNCTCC 3 cut(s) 433, 463, 626
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 224, 380
Bse1I ACTGG 2 cut(s) 385, 505
Bse21I CCTNAGG 1 cut(s) 23
BseBI CCWGG 2 cut(s) 33, 581
BseDI CCNNGG 3 cut(s) 31, 256, 273
BseGI GGATG 5 cut(s) 75, 289, 369, 419, 589
BseLI CCNNNNNNNGG 3 cut(s) 132, 224, 380
BseMII CTCAG 2 cut(s) 152, 528
BseNI ACTGG 2 cut(s) 385, 505
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 5, 196
BseYI CCCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 272
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 3 cut(s) 132, 224, 380
BsmAI GTCTC 2 cut(s) 141, 389
BsmFI GGGAC 1 cut(s) 146
Bso31I GGTCTC 2 cut(s) 141, 389
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 214, 336, 553
BspACI CCGC 1 cut(s) 591
BspCNI CTCAG 2 cut(s) 151, 529
BspLI GGNNCC 3 cut(s) 29, 233, 338
BspPI GGATC 3 cut(s) 209, 331, 344
BspT107I GGYRCC 1 cut(s) 231
BspTNI GGTCTC 2 cut(s) 141, 389
BsrI ACTGG 2 cut(s) 385, 505
BssECI CCNNGG 3 cut(s) 31, 256, 273
BssMI GATC 3 cut(s) 214, 336, 553
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 33, 581
Bst4CI ACNGT 2 cut(s) 160, 361
Bst6I CTCTTC 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 59, 63
BstDEI CTNAG 3 cut(s) 23, 138, 537
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 5 cut(s) 75, 289, 369, 419, 589
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 3 cut(s) 217, 339, 556
BstMAI GTCTC 2 cut(s) 141, 389
BstMBI GATC 3 cut(s) 214, 336, 553
BstNI CCWGG 2 cut(s) 33, 581
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 31, 579
BstV1I GCAGC 2 cut(s) 5, 196
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 5 cut(s) 75, 289, 369, 419, 589
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 459, 498, 528
Cac8I GCNNGC 2 cut(s) 59, 63
CfoI GCGC 1 cut(s) 57
Csp6I GTAC 4 cut(s) 145, 232, 496, 577
CviAII CATG 7 cut(s) 15, 103, 199, 224, 250, 511, 547
CviJI RGCY 7 cut(s) 40, 61, 152, 248, 395, 482, 536
CviKI_1 RGCY 7 cut(s) 40, 61, 152, 248, 395, 482, 536
CviQI GTAC 4 cut(s) 145, 232, 496, 577
DdeI CTNAG 3 cut(s) 23, 138, 537
DpnI GATC 3 cut(s) 216, 338, 555
DpnII GATC 3 cut(s) 214, 336, 553
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 2 cut(s) 141, 389
Eco57I CTGAAG 1 cut(s) 552
Eco81I CCTNAGG 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 31, 579
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 1 cut(s) 146
FatI CATG 7 cut(s) 14, 102, 198, 223, 249, 510, 546
Fnu4HI GCNGC 2 cut(s) 19, 210
FokI GGATG 5 cut(s) 62, 296, 356, 406, 596
Fsp4HI GCNGC 2 cut(s) 19, 210
FspI TGCGCA 1 cut(s) 56
GlaI GCGC 1 cut(s) 56
GluI GCNGC 2 cut(s) 19, 210
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 27
HhaI GCGC 1 cut(s) 57
Hin1II CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 2 cut(s) 70, 357
HindII GTYRAC 2 cut(s) 70, 357
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 188
HpaI GTTAAC 1 cut(s) 70
HphI GGTGA 3 cut(s) 163, 457, 515
Hpy166II GTNNAC 4 cut(s) 70, 357, 459, 498
Hpy188I TCNGA 4 cut(s) 49, 123, 410, 565
Hpy188III TCNNGA 6 cut(s) 6, 129, 388, 428, 439, 472
Hpy8I GTNNAC 4 cut(s) 70, 357, 459, 498
HpyAV CCTTC 4 cut(s) 91, 222, 472, 500
HpyCH4III ACNGT 2 cut(s) 160, 361
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 304, 490
HpyF3I CTNAG 3 cut(s) 23, 138, 537
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
HspAI GCGC 1 cut(s) 55
KpnI GGTACC 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 3 cut(s) 214, 336, 553
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 2 cut(s) 5, 196
LweI GCATC 2 cut(s) 251, 574
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 10, 365
MalI GATC 3 cut(s) 216, 338, 555
MboI GATC 3 cut(s) 214, 336, 553
MboII GAAGA 3 cut(s) 83, 309, 623
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 2 cut(s) 42, 69
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 593
MspR9I CCNGG 2 cut(s) 33, 581
MvaI CCWGG 2 cut(s) 33, 581
NdeII GATC 3 cut(s) 214, 336, 553
NlaIII CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
NlaIV GGNNCC 3 cut(s) 29, 233, 338
NmuCI GTSAC 1 cut(s) 365
NsbI TGCGCA 1 cut(s) 56
NspI RCATGY 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 2 cut(s) 20, 211
Psp6I CCWGG 2 cut(s) 31, 579
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 2 cut(s) 31, 579
PspN4I GGNNCC 3 cut(s) 29, 233, 338
PsrI GAACNNNNNNTAC 2 cut(s) 305, 337
PsuI RGATCY 1 cut(s) 336
RsaI GTAC 4 cut(s) 146, 233, 497, 578
RsaNI GTAC 4 cut(s) 145, 232, 496, 577
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 2 cut(s) 42, 69
SatI GCNGC 2 cut(s) 19, 210
Sau3AI GATC 3 cut(s) 214, 336, 553
ScrFI CCNGG 2 cut(s) 33, 581
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 2 cut(s) 251, 574
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 2 cut(s) 127, 386
SmoI CTYRAG 2 cut(s) 127, 386
Sse9I AATT 1 cut(s) 524
SsiI CCGC 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 31, 579
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 160, 361
TaiI ACGT 1 cut(s) 351
TasI AATT 1 cut(s) 524
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 42, 69
Tru9I TTAA 2 cut(s) 42, 69
TscAI CASTG 3 cut(s) 466, 505, 535
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 18, 209
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 6 cut(s) 91, 311, 394, 563, 624, 630
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 3 cut(s) 466, 505, 535
Van91I CCANNNNNTGG 1 cut(s) 380
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.