Rroxscaffold_1G00013720

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014835
Physical Location & Seq
Forward (+)
16936899 .. 16939797
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013720.1

Sequence Viewer

Length: 543 bp
ATGGTCGGTTTTGCTCCTCTGACCTCAAGAGGGTCTCAGCAGTACAGAGCTTTGACTGTCCCAGAACTCACCCAGCAAATGTGGGATTCCAAGAACATGATGTGTGCTGCTGATCCCCGACATGGAAGGTACCTCACAGCATCAGCCATGTTCCGTGGTAAGATGAGCACCAAGGAAGTGGATGACCAGATGATGAATGTGCAGAACAAGAACTCTTCCTACTTTGTTGAATGGATCCCCAACAACGTCAAGTCTACAGTGTGTGACATCCCACCAACTGGTCTCAAGATGGCATCAACTTTCATTGGAAACTCTACATCCATTCAAGAGATGTTCAGGAGGGTGAGTGAGCAGTTCACTGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGACGAGATGGAATTCACCGAAGCTGAGAGCAACATGAATGATCTGGTGTCAGAATATCAGCAGTACCAGGATGCCACTGCTGATGAGGATGAGTATGAAGATGAACAGGGAGAGTATGAGGAGGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

180

Amino Acids

20.84

Weight (kDa)

4.49

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 1 - 116 2.7e-36 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 278
AccI GTMKAC 1 cut(s) 254
AclWI GGATC 3 cut(s) 107, 229, 242
AcsI RAATTY 2 cut(s) 422, 537
AfaI GTAC 4 cut(s) 44, 131, 395, 476
AfiI CCNNNNNNNGG 3 cut(s) 30, 122, 278
AgsI TTSAA 2 cut(s) 230, 326
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 2 cut(s) 50, 434
AluI AGCT 2 cut(s) 50, 434
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 39, 287
AlwI GGATC 3 cut(s) 107, 229, 242
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 2 cut(s) 422, 537
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 129
AsuHPI GGTGA 4 cut(s) 61, 355, 413, 418
BaeI ACNNNNGTAYC 2 cut(s) 385, 418
BamHI GGATCC 1 cut(s) 234
BanI GGYRCC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 283, 412
BciT130I CCWGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 39, 287
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 479
BmiI GGNNCC 2 cut(s) 131, 236
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 3 cut(s) 149, 302, 472
BpuEI CTTGAG 2 cut(s) 10, 269
BsaI GGTCTC 2 cut(s) 39, 287
BsaJI CCNNGG 2 cut(s) 154, 171
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 122, 278
Bse1I ACTGG 2 cut(s) 283, 403
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 2 cut(s) 154, 171
BseGI GGATG 5 cut(s) 187, 267, 317, 487, 505
BseLI CCNNNNNNNGG 3 cut(s) 30, 122, 278
BseMII CTCAG 2 cut(s) 50, 426
BseNI ACTGG 2 cut(s) 283, 403
BseRI GAGGAG 1 cut(s) 6
BseXI GCAGC 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 72
BsgI GTGCAG 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 170
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 3 cut(s) 30, 122, 278
BsmAI GTCTC 2 cut(s) 39, 287
BsmFI GGGAC 1 cut(s) 44
Bso31I GGTCTC 2 cut(s) 39, 287
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 3 cut(s) 112, 234, 451
BspCNI CTCAG 2 cut(s) 49, 427
BspLI GGNNCC 2 cut(s) 131, 236
BspPI GGATC 3 cut(s) 107, 229, 242
BspT107I GGYRCC 1 cut(s) 129
BspTNI GGTCTC 2 cut(s) 39, 287
BsrI ACTGG 2 cut(s) 283, 403
BssECI CCNNGG 2 cut(s) 154, 171
BssMI GATC 3 cut(s) 112, 234, 451
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 2 cut(s) 58, 259
Bst6I CTCTTC 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 36, 435
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 5 cut(s) 187, 267, 317, 487, 505
BstKTI GATC 3 cut(s) 115, 237, 454
BstMAI GTCTC 2 cut(s) 39, 287
BstMBI GATC 3 cut(s) 112, 234, 451
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 178
BstYI RGATCY 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 154
BtsCI GGATG 5 cut(s) 187, 267, 317, 487, 505
BtsI GCAGTG 2 cut(s) 357, 486
BtsIMutI CAGTG 4 cut(s) 264, 357, 396, 486
Csp6I GTAC 4 cut(s) 43, 130, 394, 475
CviAII CATG 5 cut(s) 97, 122, 148, 409, 445
CviJI RGCY 4 cut(s) 50, 146, 380, 434
CviKI_1 RGCY 4 cut(s) 50, 146, 380, 434
CviQI GTAC 4 cut(s) 43, 130, 394, 475
DdeI CTNAG 2 cut(s) 36, 435
DpnI GATC 3 cut(s) 114, 236, 453
DpnII GATC 3 cut(s) 112, 234, 451
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 39, 287
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 5 cut(s) 100, 125, 151, 412, 448
FaiI YATR 8 cut(s) 98, 123, 149, 365, 410, 446, 507, 528
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 44
FatI CATG 5 cut(s) 96, 121, 147, 408, 444
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 5 cut(s) 194, 254, 304, 494, 512
Fsp4HI GCNGC 1 cut(s) 108
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 76
Hin1II CATG 5 cut(s) 100, 125, 151, 412, 448
HinfI GANTC 1 cut(s) 86
HphI GGTGA 4 cut(s) 61, 355, 413, 418
Hpy166II GTNNAC 3 cut(s) 255, 357, 396
Hpy188I TCNGA 2 cut(s) 21, 463
Hpy188III TCNNGA 5 cut(s) 27, 286, 326, 337, 370
Hpy8I GTNNAC 3 cut(s) 255, 357, 396
HpyAV CCTTC 3 cut(s) 120, 370, 398
HpyCH4III ACNGT 2 cut(s) 58, 259
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 2 cut(s) 202, 388
HpyF3I CTNAG 2 cut(s) 36, 435
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 5 cut(s) 100, 125, 151, 412, 448
KpnI GGTACC 1 cut(s) 133
Kzo9I GATC 3 cut(s) 112, 234, 451
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 3 cut(s) 149, 302, 472
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 263
MalI GATC 3 cut(s) 114, 236, 453
MboI GATC 3 cut(s) 112, 234, 451
MboII GAAGA 2 cut(s) 207, 521
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 2 cut(s) 422, 537
MnlI CCTC 9 cut(s) 23, 27, 34, 143, 333, 366, 490, 523, 526
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 541
MslI CAYNNNNRTG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 479
NdeII GATC 3 cut(s) 112, 234, 451
NlaIII CATG 5 cut(s) 100, 125, 151, 412, 448
NlaIV GGNNCC 2 cut(s) 131, 236
NmuCI GTSAC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 86
PflMI CCANNNNNTGG 1 cut(s) 278
PkrI GCNGC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 477
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 131, 236
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 4 cut(s) 44, 131, 395, 476
RsaNI GTAC 4 cut(s) 43, 130, 394, 475
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 541
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 3 cut(s) 112, 234, 451
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 170
SetI ASST 6 cut(s) 26, 52, 131, 135, 249, 436
SfaNI GCATC 3 cut(s) 149, 302, 472
SfcI CTRYAG 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 25, 284
SmoI CTYRAG 2 cut(s) 25, 284
Sse9I AATT 2 cut(s) 422, 537
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 58, 259
TaiI ACGT 1 cut(s) 249
TasI AATT 2 cut(s) 422, 537
TatI WGTACW 1 cut(s) 42
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 4 cut(s) 264, 364, 403, 493
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 209, 292, 461, 522, 528
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 4 cut(s) 264, 364, 403, 493
Van91I CCANNNNNTGG 1 cut(s) 278
XapI RAATTY 2 cut(s) 422, 537
XmiI GTMKAC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.