Rh5CG483200

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014835
Physical Location & Seq
Reverse (-)
67359302 .. 67362549
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG483200.1

Sequence Viewer

Length: 903 bp
ATGGGAACGCTTCTGATTTCAAAGATTAGGGAAGAGTACCCAGATCGGATGATGATGACCTTCTCTGTGTTCCCATCTCCCAAGGTCTCAGATACTGTTGTGGAGCCTTACAATGCTACTTTGTCTGTTCATCAGCTTGTAGAGAATGCAGATGAGTGTATGGTGCTTGACAATGAGGCTCTCTATGACATTTGCTTCCGCACACTTAAACTCACTACCCCCAGCTTTGGTGATTTGAATCACTTAATCTCTGCAACAATGTCTGGAGTTACATGCTGCCTAAGGTTCCCTGGACAGCTTAACTCTGATTTGCGCAAGCTTGCTGTTAACCTCATCCCCTTCCCTCGTCTTCACTTTTTCATGGTCGGTTTTGCTCCTCTGACCTCAAGAGGGTCTCAGCAGTACAGAGCTTTGACTGTCCCAGAACTCACCCAGCAAATGTGGGATTCCAAGAACATGATGTGTGCTGCTGATCCCCGACATGGAAGGTACCTCACAGCATCAGCCATGTTCCGTGGTAAGATGAGCACCAAGGAAGTGGATGACCAGATGATGAATGTGCAGAACAAGAACTCTTCCTACTTTGTTGAGTGGATCCCCAACAACGTCAAGTCAACAGTCTGTGACATCCCACCAACTGGTCTCAAGATGGCTTCAACTTTCATCGGAAACTCTACATCCATTCAAGAGATGTTCAGGAGGGTGAGTGAGCAGTTCACTGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGACGAGATGGAATTCACTGAAGCTGAGAGCAACATGAATGATCTGGTGTCAGAATATCAGCAGTACCAGGATGCCACCGCTGATGAGGACGAGTATGAAGATGAACAGGGAGAGTATGAGGAGGAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

300

Amino Acids

34.43

Weight (kDa)

4.71

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 2 - 65 7.3e-15 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 115 - 236 7.2e-41 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 314
Acc65I GGTACC 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 489
AccB7I CCANNNNNTGG 1 cut(s) 638
AciI CCGC 2 cut(s) 199, 849
AclWI GGATC 3 cut(s) 467, 589, 602
AcsI RAATTY 1 cut(s) 782
AcuI CTGAAG 1 cut(s) 810
AfaI GTAC 5 cut(s) 38, 404, 491, 755, 836
AfiI CCNNNNNNNGG 4 cut(s) 227, 390, 482, 638
AgsI TTSAA 4 cut(s) 21, 238, 657, 686
AjnI CCWGG 2 cut(s) 289, 837
AluBI AGCT 6 cut(s) 136, 225, 298, 319, 410, 794
AluI AGCT 6 cut(s) 136, 225, 298, 319, 410, 794
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 3 cut(s) 91, 399, 647
AlwI GGATC 3 cut(s) 467, 589, 602
AlwNI CAGNNNCTG 1 cut(s) 95
ApeKI GCWGC 2 cut(s) 276, 467
ApoI RAATTY 1 cut(s) 782
Asp700I GAANNNNTTC 1 cut(s) 740
Asp718I GGTACC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 315
AsuHPI GGTGA 4 cut(s) 242, 421, 715, 773
AxyI CCTNAGG 1 cut(s) 281
BaeI ACNNNNGTAYC 4 cut(s) 84, 117, 745, 778
BamHI GGATCC 1 cut(s) 594
BanI GGYRCC 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 2 cut(s) 263, 454
BccI CCATC 3 cut(s) 82, 643, 772
BciT130I CCWGG 2 cut(s) 291, 839
BcoDI GTCTC 3 cut(s) 91, 399, 647
BisI GCNGC 2 cut(s) 277, 468
BlsI GCNGC 2 cut(s) 278, 469
Bme1390I CCNGG 2 cut(s) 291, 839
BmiI GGNNCC 4 cut(s) 105, 287, 491, 596
BmrFI CCNGG 2 cut(s) 291, 839
BmsI GCATC 2 cut(s) 509, 832
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 370, 629
BsaBI GATNNNNATC 1 cut(s) 237
BsaI GGTCTC 3 cut(s) 91, 399, 647
BsaJI CCNNGG 4 cut(s) 81, 289, 514, 531
BsaXI ACNNNNNCTCC 3 cut(s) 691, 721, 884
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 390, 482, 638
Bse1I ACTGG 2 cut(s) 643, 763
Bse21I CCTNAGG 1 cut(s) 281
Bse8I GATNNNNATC 1 cut(s) 237
BseBI CCWGG 2 cut(s) 291, 839
BseDI CCNNGG 4 cut(s) 81, 289, 514, 531
BseGI GGATG 6 cut(s) 54, 333, 547, 627, 677, 847
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 4 cut(s) 227, 390, 482, 638
BseMII CTCAG 3 cut(s) 102, 410, 786
BseNI ACTGG 2 cut(s) 643, 763
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 2 cut(s) 263, 454
BseYI CCCAGC 2 cut(s) 221, 432
BsgI GTGCAG 1 cut(s) 581
BshNI GGYRCC 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 530
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 4 cut(s) 227, 390, 482, 638
BsmAI GTCTC 3 cut(s) 91, 399, 647
BsmFI GGGAC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 151
Bso31I GGTCTC 3 cut(s) 91, 399, 647
Bsp1286I GDGCHC 1 cut(s) 530
Bsp143I GATC 4 cut(s) 43, 472, 594, 811
BspACI CCGC 2 cut(s) 199, 849
BspCNI CTCAG 3 cut(s) 101, 409, 787
BspLI GGNNCC 4 cut(s) 105, 287, 491, 596
BspPI GGATC 3 cut(s) 467, 589, 602
BspT107I GGYRCC 1 cut(s) 489
BspTNI GGTCTC 3 cut(s) 91, 399, 647
BsrI ACTGG 2 cut(s) 643, 763
BssECI CCNNGG 4 cut(s) 81, 289, 514, 531
BssMI GATC 4 cut(s) 43, 472, 594, 811
BssT1I CCWWGG 2 cut(s) 81, 531
Bst2UI CCWGG 2 cut(s) 291, 839
Bst4CI ACNGT 3 cut(s) 97, 418, 619
Bst6I CTCTTC 2 cut(s) 27, 580
BstC8I GCNNGC 2 cut(s) 317, 321
BstDEI CTNAG 4 cut(s) 88, 281, 396, 795
BstDSI CCRYGG 1 cut(s) 514
BstF5I GGATG 6 cut(s) 54, 333, 547, 627, 677, 847
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 4 cut(s) 46, 475, 597, 814
BstMAI GTCTC 3 cut(s) 91, 399, 647
BstMBI GATC 4 cut(s) 43, 472, 594, 811
BstNI CCWGG 2 cut(s) 291, 839
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 289, 837
BstV1I GCAGC 2 cut(s) 263, 454
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 594
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 1 cut(s) 594
Bsu36I CCTNAGG 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 6 cut(s) 54, 333, 547, 627, 677, 847
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 3 cut(s) 717, 756, 786
Cac8I GCNNGC 2 cut(s) 317, 321
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 315
Csp6I GTAC 5 cut(s) 37, 403, 490, 754, 835
CviAII CATG 7 cut(s) 273, 361, 457, 482, 508, 769, 805
CviQI GTAC 5 cut(s) 37, 403, 490, 754, 835
DdeI CTNAG 4 cut(s) 88, 281, 396, 795
DpnI GATC 4 cut(s) 45, 474, 596, 813
DpnII GATC 4 cut(s) 43, 472, 594, 811
Eam1104I CTCTTC 2 cut(s) 27, 580
EarI CTCTTC 2 cut(s) 27, 580
Eco130I CCWWGG 2 cut(s) 81, 531
Eco31I GGTCTC 3 cut(s) 91, 399, 647
Eco57I CTGAAG 1 cut(s) 810
Eco81I CCTNAGG 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 289, 837
EcoT14I CCWWGG 2 cut(s) 81, 531
ErhI CCWWGG 2 cut(s) 81, 531
FaeI CATG 7 cut(s) 276, 364, 460, 485, 511, 772, 808
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 1 cut(s) 404
FatI CATG 7 cut(s) 272, 360, 456, 481, 507, 768, 804
Fnu4HI GCNGC 2 cut(s) 277, 468
FokI GGATG 6 cut(s) 61, 320, 554, 614, 664, 854
Fsp4HI GCNGC 2 cut(s) 277, 468
FspI TGCGCA 1 cut(s) 314
GlaI GCGC 1 cut(s) 314
GluI GCNGC 2 cut(s) 277, 468
GsaI CCCAGC 2 cut(s) 225, 436
GsuI CTGGAG 1 cut(s) 285
HhaI GCGC 1 cut(s) 315
Hin1II CATG 7 cut(s) 276, 364, 460, 485, 511, 772, 808
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HincII GTYRAC 2 cut(s) 328, 615
HindII GTYRAC 2 cut(s) 328, 615
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 2 cut(s) 238, 446
HpaI GTTAAC 1 cut(s) 328
HphI GGTGA 4 cut(s) 242, 421, 715, 773
Hpy166II GTNNAC 4 cut(s) 328, 615, 717, 756
Hpy188I TCNGA 7 cut(s) 15, 48, 91, 307, 381, 668, 823
Hpy188III TCNNGA 6 cut(s) 264, 387, 646, 686, 697, 730
Hpy8I GTNNAC 4 cut(s) 328, 615, 717, 756
HpyAV CCTTC 5 cut(s) 70, 349, 480, 730, 758
HpyCH4III ACNGT 3 cut(s) 97, 418, 619
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 4 cut(s) 149, 254, 562, 748
HpyF3I CTNAG 4 cut(s) 88, 281, 396, 795
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 7 cut(s) 276, 364, 460, 485, 511, 772, 808
HspAI GCGC 1 cut(s) 313
KpnI GGTACC 1 cut(s) 493
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 4 cut(s) 43, 472, 594, 811
LmnI GCTCC 2 cut(s) 103, 379
Lsp1109I GCAGC 2 cut(s) 263, 454
LweI GCATC 2 cut(s) 509, 832
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 2 cut(s) 268, 623
MalI GATC 4 cut(s) 45, 474, 596, 813
MboI GATC 4 cut(s) 43, 472, 594, 811
MboII GAAGA 4 cut(s) 44, 341, 567, 881
MflI RGATCY 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 530
MluCI AATT 1 cut(s) 782
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 4 cut(s) 207, 245, 300, 327
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 851
MspR9I CCNGG 2 cut(s) 291, 839
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 2 cut(s) 291, 839
NdeII GATC 4 cut(s) 43, 472, 594, 811
NlaIII CATG 7 cut(s) 276, 364, 460, 485, 511, 772, 808
NlaIV GGNNCC 4 cut(s) 105, 287, 491, 596
NmuCI GTSAC 1 cut(s) 623
NsbI TGCGCA 1 cut(s) 314
NspI RCATGY 1 cut(s) 276
PctI GAATGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 2 cut(s) 238, 446
PflMI CCANNNNNTGG 1 cut(s) 638
PkrI GCNGC 2 cut(s) 278, 469
Psp6I CCWGG 2 cut(s) 289, 837
PspFI CCCAGC 2 cut(s) 221, 432
PspGI CCWGG 2 cut(s) 289, 837
PspN4I GGNNCC 4 cut(s) 105, 287, 491, 596
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 594
RsaI GTAC 5 cut(s) 38, 404, 491, 755, 836
RsaNI GTAC 5 cut(s) 37, 403, 490, 754, 835
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 4 cut(s) 207, 245, 300, 327
SatI GCNGC 2 cut(s) 277, 468
Sau3AI GATC 4 cut(s) 43, 472, 594, 811
ScrFI CCNGG 2 cut(s) 291, 839
SduI GDGCHC 1 cut(s) 530
SfaNI GCATC 2 cut(s) 509, 832
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 2 cut(s) 385, 644
SmoI CTYRAG 2 cut(s) 385, 644
Sse9I AATT 1 cut(s) 782
SsiI CCGC 2 cut(s) 199, 849
StyD4I CCNGG 2 cut(s) 289, 837
StyI CCWWGG 2 cut(s) 81, 531
TaaI ACNGT 3 cut(s) 97, 418, 619
TaiI ACGT 1 cut(s) 609
TasI AATT 1 cut(s) 782
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 2 cut(s) 238, 446
Tru1I TTAA 4 cut(s) 207, 245, 300, 327
Tru9I TTAA 4 cut(s) 207, 245, 300, 327
TscAI CASTG 3 cut(s) 724, 763, 793
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 2 cut(s) 276, 467
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 7 cut(s) 119, 349, 569, 652, 821, 882, 888
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 3 cut(s) 724, 763, 793
Van91I CCANNNNNTGG 1 cut(s) 638
XapI RAATTY 1 cut(s) 782
XceI RCATGY 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.