Rh3BG132000

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0014835
Physical Location & Seq
Forward (+)
11122168 .. 11125373
3206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG132000.1

Sequence Viewer

Length: 693 bp
ATGTTTTGTACAGTTGGGGATTTGAACCATTTGATTTCTGCTACCATGTCTGGTGTGACTTGCTGTTTGAGGTTCCCTGGGCAACTCAACTCTGACCTCAGAAAGTTGGCTGTCAATCTCATTCCATTCCCCCGTCTCCACTTTTTCATGGTGGGTTTTGCTCCTTTGACCTCTCGTGGTTCACAGATGTACAGAGCCTTGACTGTGCCAGAACTCACTCAGCAAATGTGGGATTCTAAGAACATGATGTGCGCAGCTGACCCTCGCCATGGTCGCTATCTCACTGCCTCAGCCATGTTCAGGGGAAAGATGAGCACCAAGGAAGTCGATGATCAAATGAGCAATGTTCAGAACAAGAACTCTTCATACTTTGTGGAGTGGATTCCCCACAATGTCAAGTCCACTGTTTGTGATATCCCACCGACAGGCTTGAAGATGGCATCAACCTTCATTGGAAACTCAACTTCAATTCAGGAGATGTTCCGTCGTGTGAGTGAGCAGTTCACTGCTATGTTCAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAAGGCATGGATGAGATGGAGTTCACAGAGGCTGAAAGCAACATGAATGATCTTGTTTCAGAGTATCAGCAGTACCAGGATGCAACAGCTGAGGAGGATGAGTACTATGAAGAGGAAGAGGAAGAAGAAGCTCAGGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

230

Amino Acids

26.49

Weight (kDa)

4.81

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 44 - 165 3.8e-41 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 253
AfaI GTAC 5 cut(s) 10, 191, 542, 623, 653
AfiI CCNNNNNNNGG 3 cut(s) 269, 300, 425
AgsI TTSAA 3 cut(s) 25, 433, 468
AjnI CCWGG 2 cut(s) 76, 624
AluBI AGCT 3 cut(s) 257, 638, 680
AluI AGCT 3 cut(s) 257, 638, 680
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 581
ApeKI GCWGC 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 560
BaeI ACNNNNGTAYC 2 cut(s) 532, 565
BauI CACGAG 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 317
BbvCI CCTCAGC 2 cut(s) 289, 639
BbvI GCAGC 1 cut(s) 266
BccI CCATC 2 cut(s) 430, 559
BciT130I CCWGG 2 cut(s) 78, 626
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 140
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
BmcAI AGTACT 1 cut(s) 653
Bme1390I CCNGG 2 cut(s) 78, 626
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 78, 626
BmsI GCATC 2 cut(s) 449, 619
Bpu10I CCTNAGC 3 cut(s) 289, 639, 681
BsaJI CCNNGG 4 cut(s) 76, 77, 268, 318
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 300, 425
Bse1I ACTGG 1 cut(s) 550
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 2 cut(s) 78, 626
BseDI CCNNGG 4 cut(s) 76, 77, 268, 318
BseGI GGATG 3 cut(s) 565, 634, 652
BseLI CCNNNNNNNGG 3 cut(s) 269, 300, 425
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 4 cut(s) 112, 233, 303, 630
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 269, 300, 425
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 317
Bsp1407I TGTACA 2 cut(s) 8, 189
Bsp143I GATC 2 cut(s) 331, 598
Bsp19I CCATGG 1 cut(s) 268
BspCNI CTCAG 4 cut(s) 111, 232, 302, 631
BspLI GGNNCC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 349
BsrGI TGTACA 2 cut(s) 8, 189
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 4 cut(s) 76, 77, 268, 318
BssMI GATC 2 cut(s) 331, 598
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 268, 318
Bst2BI CACGAG 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 78, 626
Bst4CI ACNGT 3 cut(s) 13, 205, 406
Bst6I CTCTTC 3 cut(s) 367, 654, 660
BstAUI TGTACA 2 cut(s) 8, 189
BstDEI CTNAG 6 cut(s) 98, 219, 237, 289, 639, 681
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 565, 634, 652
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 2 cut(s) 334, 601
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 2 cut(s) 331, 598
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 2 cut(s) 78, 626
BstSCI CCNGG 2 cut(s) 76, 624
BstV1I GCAGC 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 565, 634, 652
BtsI GCAGTG 2 cut(s) 282, 504
BtsIMutI CAGTG 4 cut(s) 282, 402, 504, 543
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 1 cut(s) 254
Csp6I GTAC 5 cut(s) 9, 190, 541, 622, 652
CviAII CATG 7 cut(s) 46, 148, 244, 269, 295, 556, 592
CviJI RGCY 9 cut(s) 110, 197, 257, 293, 429, 527, 581, 638, 680
CviKI_1 RGCY 9 cut(s) 110, 197, 257, 293, 429, 527, 581, 638, 680
CviQI GTAC 5 cut(s) 9, 190, 541, 622, 652
DdeI CTNAG 6 cut(s) 98, 219, 237, 289, 639, 681
DpnI GATC 2 cut(s) 333, 600
DpnII GATC 2 cut(s) 331, 598
Eam1104I CTCTTC 3 cut(s) 367, 654, 660
EarI CTCTTC 3 cut(s) 367, 654, 660
Eco130I CCWWGG 2 cut(s) 268, 318
Eco32I GATATC 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 76, 624
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 268, 318
ErhI CCWWGG 2 cut(s) 268, 318
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 7 cut(s) 49, 151, 247, 272, 298, 559, 595
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 7 cut(s) 45, 147, 243, 268, 294, 555, 591
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 3 cut(s) 572, 641, 659
Fsp4HI GCNGC 1 cut(s) 255
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 1 cut(s) 253
GluI GCNGC 1 cut(s) 255
HhaI GCGC 1 cut(s) 254
Hin1II CATG 7 cut(s) 49, 151, 247, 272, 298, 559, 595
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 2 cut(s) 233, 382
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 5 cut(s) 182, 402, 504, 543, 573
Hpy188I TCNGA 4 cut(s) 94, 101, 351, 610
Hpy188III TCNNGA 3 cut(s) 473, 517, 683
Hpy8I GTNNAC 5 cut(s) 182, 402, 504, 543, 573
Hpy99I CGWCG 1 cut(s) 489
HpyAV CCTTC 3 cut(s) 457, 517, 545
HpyCH4III ACNGT 3 cut(s) 13, 205, 406
HpyCH4V TGCA 2 cut(s) 535, 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 6 cut(s) 98, 219, 237, 289, 639, 681
Hsp92II CATG 7 cut(s) 49, 151, 247, 272, 298, 559, 595
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 2 cut(s) 331, 598
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 1 cut(s) 266
LweI GCATC 2 cut(s) 449, 619
MaeIII GTNAC 1 cut(s) 55
MalI GATC 2 cut(s) 333, 600
MboI GATC 2 cut(s) 331, 598
MboII GAAGA 6 cut(s) 354, 445, 671, 677, 683, 686
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 1 cut(s) 468
MroXI GAANNNNTTC 1 cut(s) 527
MslI CAYNNNNRTG 1 cut(s) 509
MspA1I CMGCKG 2 cut(s) 257, 638
MspR9I CCNGG 2 cut(s) 78, 626
MvaI CCWGG 2 cut(s) 78, 626
MwoI GCNNNNNNNGC 1 cut(s) 273
NcoI CCATGG 1 cut(s) 268
NdeII GATC 2 cut(s) 331, 598
NlaIII CATG 7 cut(s) 49, 151, 247, 272, 298, 559, 595
NlaIV GGNNCC 1 cut(s) 74
NmuCI GTSAC 1 cut(s) 55
NsbI TGCGCA 1 cut(s) 253
PasI CCCWGGG 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 2 cut(s) 233, 382
PkrI GCNGC 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 76, 624
PspGI CCWGG 2 cut(s) 76, 624
PspN4I GGNNCC 1 cut(s) 74
PsrI GAACNNNNNNTAC 2 cut(s) 350, 382
PstNI CAGNNNCTG 1 cut(s) 581
PvuII CAGCTG 2 cut(s) 257, 638
RsaI GTAC 5 cut(s) 10, 191, 542, 623, 653
RsaNI GTAC 5 cut(s) 9, 190, 541, 622, 652
RseI CAYNNNNRTG 1 cut(s) 509
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 331, 598
ScaI AGTACT 1 cut(s) 653
ScrFI CCNGG 2 cut(s) 78, 626
SduI GDGCHC 1 cut(s) 317
SetI ASST 7 cut(s) 74, 99, 173, 259, 449, 640, 682
SfaNI GCATC 2 cut(s) 449, 619
SmiMI CAYNNNNRTG 1 cut(s) 509
Sse9I AATT 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 76, 624
StyI CCWWGG 2 cut(s) 268, 318
TaaI ACNGT 3 cut(s) 13, 205, 406
TaqI TCGA 1 cut(s) 327
TasI AATT 1 cut(s) 468
TatI WGTACW 3 cut(s) 8, 189, 651
TfiI GAWTC 2 cut(s) 233, 382
TscAI CASTG 4 cut(s) 289, 409, 511, 550
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 5 cut(s) 136, 354, 439, 608, 672
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 4 cut(s) 289, 409, 511, 550
XmnI GAANNNNTTC 1 cut(s) 527
ZrmI AGTACT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.