pycom10g20830

GDP-L-galactose phosphorylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010539
Physical Location & Seq
Forward (+)
23611059 .. 23613144
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20830.6

Sequence Viewer

Length: 1131 bp
ATGGTTACGGTTAAGCAGCTTCAAGATTGTAACTTTACGTCGCAATTCCCTCCATTTTCTCTGCAAGGTATAAAAATCCCCTTATACCGTTTTAGTAGCAATTCGATACTGGATAATGGTTGTGTTGGAGTCATTTCGTGTGTTCTCGAAGAAGAAGGAGGAGGAGAAGAAGAAGAAGAAGAACAGAGTGTACTGGATTCTATTCTTCTTGCGCAGTGGGAAGATCGAATGTGGAAAGGTCTGTTCAGATACGATGTGACAACATCTGAAATCAAGGTCATCGGTGGCAGGAAGAAGTTTATTGCTCAGTTTAACGAAGGATGGAGTAGGGATTTTATTCCAAGGATGGGCAAGAACAAGAATTTCTGCCAAGAAGATGCGTTCAAGTTTAGTTGGATGGAACGGCAAGAGGAGCTACTCTTTTGTGTTTCAAGCGGTGAAAAGTCGAACCCTGAGCTTGTTCTTTCAGCTGCTGTGCCTGATTGTGCCTTATTGATTGCTATCAATGCATCCCCTGTGGAATATGGTCATGTCTTGTTGGTACCACATGGCTCTAAAATTATTCACCAGTACCTCGATAAAAGGTCATTGGAACTTGTCCTGCGCATTGCTGTTGAAATAAATAATTGCTCTTTTCACCTATTCTATGACTATTCACCTAGTGCTTCTCCTTTATATTTCCAGGCTTGCTACCTTCAAATCCCTTTGCCTGTTGAGTTCATGCATTTCGATACAATATTTGGTGCTGGGAAAGAGGGGATGCGCATCTCTTGTGTTGCAGATTACCCCATTAAGACCCTCGTATTGGAGAGCAACCACTTTAATACTATGATGGAGGTTCTTGCTGAGATATCTTCGTGTTTACAGCAAAAGCTCATTCCATACAATCTGCTAATATCTGATCATGGCAAAAGGATATTCTTATTTTTCCAGCTACATACATTAAAAGACTCTTGTACCCTTTCTGCTTGGGAGTGTGGGGGGTATTTCTTGTTTAAATCGAGGTCTGAATTTGATCAAGCTACTGAGCAGGCTCTGCTAAAACAGCTAAGTACCGTCTCCCTTGACGACAAAGGTTTCCAAGCAGTGAAGTTGCTCTGTTGCAGCATTGCGGGTAAGTTTGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 ko01110 map00053 map01100 map01110
Pfam Domains
Protein Families
No domains found.

Protein Analysis

377

Amino Acids

42.62

Weight (kDa)

5.23

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 213, 605, 764
Acc65I GGTACC 1 cut(s) 541
AccB1I GGYRCC 1 cut(s) 541
AciI CCGC 2 cut(s) 435, 1112
AcsI RAATTY 2 cut(s) 361, 1010
AdeI CACNNNGTG 1 cut(s) 662
AfaI GTAC 5 cut(s) 192, 543, 572, 958, 1054
AfiI CCNNNNNNNGG 2 cut(s) 347, 805
AgsI TTSAA 5 cut(s) 23, 385, 432, 617, 698
AjnI CCWGG 1 cut(s) 681
AluBI AGCT 8 cut(s) 19, 415, 457, 470, 874, 934, 1022, 1048
AluI AGCT 8 cut(s) 19, 415, 457, 470, 874, 934, 1022, 1048
Alw26I GTCTC 1 cut(s) 1063
AlwNI CAGNNNCTG 2 cut(s) 473, 1036
ApeKI GCWGC 3 cut(s) 16, 470, 1104
ApoI RAATTY 2 cut(s) 361, 1010
Asp718I GGTACC 1 cut(s) 541
AspLEI GCGC 3 cut(s) 214, 606, 765
AsuHPI GGTGA 4 cut(s) 449, 557, 629, 648
BaeI ACNNNNGTAYC 2 cut(s) 940, 973
BanI GGYRCC 1 cut(s) 541
BbvI GCAGC 3 cut(s) 28, 457, 1116
BccI CCATC 4 cut(s) 315, 340, 391, 826
BceAI ACGGC 1 cut(s) 419
BciT130I CCWGG 1 cut(s) 683
BclI TGATCA 2 cut(s) 901, 1015
BcoDI GTCTC 1 cut(s) 1063
BfaI CTAG 1 cut(s) 660
BisI GCNGC 3 cut(s) 17, 471, 1105
BlsI GCNGC 3 cut(s) 18, 472, 1106
Bme1390I CCNGG 1 cut(s) 683
BmiI GGNNCC 1 cut(s) 543
BmrFI CCNGG 1 cut(s) 683
BmsI GCATC 4 cut(s) 367, 518, 750, 774
Bpu10I CCTNAGC 1 cut(s) 453
BsaBI GATNNNNATC 2 cut(s) 500, 764
BsaJI CCNNGG 1 cut(s) 341
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 805
Bse1I ACTGG 3 cut(s) 114, 198, 568
Bse3DI GCAATG 2 cut(s) 606, 1107
Bse8I GATNNNNATC 2 cut(s) 500, 764
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 5 cut(s) 326, 351, 402, 509, 765
BseJI GATNNNNATC 2 cut(s) 500, 764
BseLI CCNNNNNNNGG 2 cut(s) 347, 805
BseMI GCAATG 2 cut(s) 606, 1107
BseMII CTCAG 4 cut(s) 320, 444, 837, 1017
BseNI ACTGG 3 cut(s) 114, 198, 568
BseRI GAGGAG 3 cut(s) 174, 177, 425
BseXI GCAGC 3 cut(s) 28, 457, 1116
BseYI CCCAGC 1 cut(s) 746
BshNI GGYRCC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 347, 805
BsmAI GTCTC 1 cut(s) 1063
BsmBI CGTCTC 1 cut(s) 1063
Bsp143I GATC 3 cut(s) 223, 901, 1015
BspACI CCGC 2 cut(s) 435, 1112
BspCNI CTCAG 4 cut(s) 319, 445, 838, 1018
BspLI GGNNCC 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 541
BsrDI GCAATG 2 cut(s) 606, 1107
BsrI ACTGG 3 cut(s) 114, 198, 568
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 3 cut(s) 223, 901, 1015
BssT1I CCWWGG 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 10, 89, 1057
BstAPI GCANNNNNTGC 1 cut(s) 1036
BstC8I GCNNGC 2 cut(s) 688, 1032
BstDEI CTNAG 5 cut(s) 306, 453, 846, 1026, 1049
BstF5I GGATG 5 cut(s) 326, 351, 402, 509, 765
BstHHI GCGC 3 cut(s) 214, 606, 765
BstKTI GATC 3 cut(s) 226, 904, 1018
BstMAI GTCTC 1 cut(s) 1063
BstMBI GATC 3 cut(s) 223, 901, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 412, 506, 1036, 1045
BstNI CCWGG 1 cut(s) 683
BstSCI CCNGG 1 cut(s) 681
BstV1I GCAGC 3 cut(s) 28, 457, 1116
BtsCI GGATG 5 cut(s) 326, 351, 402, 509, 765
BtsI GCAGTG 2 cut(s) 221, 1092
BtsIMutI CAGTG 2 cut(s) 221, 1092
Cac8I GCNNGC 2 cut(s) 688, 1032
CaiI CAGNNNCTG 2 cut(s) 473, 1036
CfoI GCGC 3 cut(s) 214, 606, 765
Csp6I GTAC 5 cut(s) 191, 542, 571, 957, 1053
CviAII CATG 4 cut(s) 530, 548, 721, 905
CviQI GTAC 5 cut(s) 191, 542, 571, 957, 1053
DdeI CTNAG 5 cut(s) 306, 453, 846, 1026, 1049
DpnI GATC 3 cut(s) 225, 903, 1017
DpnII GATC 3 cut(s) 223, 901, 1015
DraI TTTAAA 1 cut(s) 997
DraIII CACNNNGTG 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 341
Eco32I GATATC 1 cut(s) 852
EcoRII CCWGG 1 cut(s) 681
EcoRV GATATC 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 341
EcoT22I ATGCAT 2 cut(s) 511, 726
ErhI CCWWGG 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 1063
FaeI CATG 4 cut(s) 533, 551, 724, 908
FatI CATG 4 cut(s) 529, 547, 720, 904
FauI CCCGC 1 cut(s) 1105
FbaI TGATCA 2 cut(s) 901, 1015
Fnu4HI GCNGC 3 cut(s) 17, 471, 1105
FokI GGATG 5 cut(s) 333, 358, 409, 496, 772
Fsp4HI GCNGC 3 cut(s) 17, 471, 1105
FspAI RTGCGCAY 1 cut(s) 764
FspBI CTAG 1 cut(s) 660
FspI TGCGCA 3 cut(s) 213, 605, 764
GlaI GCGC 3 cut(s) 213, 605, 764
GluI GCNGC 3 cut(s) 17, 471, 1105
GsaI CCCAGC 1 cut(s) 750
HhaI GCGC 3 cut(s) 214, 606, 765
Hin1II CATG 4 cut(s) 533, 551, 724, 908
Hin6I GCGC 3 cut(s) 212, 604, 763
HinP1I GCGC 3 cut(s) 212, 604, 763
HinfI GANTC 3 cut(s) 129, 197, 950
HphI GGTGA 4 cut(s) 449, 557, 629, 648
Hpy166II GTNNAC 2 cut(s) 191, 863
Hpy188I TCNGA 4 cut(s) 248, 268, 901, 1009
Hpy188III TCNNGA 2 cut(s) 23, 146
Hpy8I GTNNAC 2 cut(s) 191, 863
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 3 cut(s) 149, 311, 704
HpyCH4III ACNGT 3 cut(s) 10, 89, 1057
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 5 cut(s) 64, 509, 724, 779, 1104
HpyF10VI GCNNNNNNNGC 4 cut(s) 412, 506, 1036, 1045
HpyF3I CTNAG 5 cut(s) 306, 453, 846, 1026, 1049
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 4 cut(s) 533, 551, 724, 908
HspAI GCGC 3 cut(s) 212, 604, 763
KpnI GGTACC 1 cut(s) 545
Ksp22I TGATCA 2 cut(s) 901, 1015
Kzo9I GATC 3 cut(s) 223, 901, 1015
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 3 cut(s) 28, 457, 1116
LweI GCATC 4 cut(s) 367, 518, 750, 774
MaeI CTAG 1 cut(s) 660
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 4, 29, 256
MalI GATC 3 cut(s) 225, 903, 1017
MboI GATC 3 cut(s) 223, 901, 1015
MluCI AATT 6 cut(s) 44, 100, 361, 558, 625, 1010
MlyI GAGTC 2 cut(s) 138, 944
MmeI TCCRAC 2 cut(s) 106, 374
MnlI CCTC 9 cut(s) 60, 152, 155, 403, 584, 748, 809, 829, 996
Mph1103I ATGCAT 2 cut(s) 511, 726
MseI TTAA 6 cut(s) 12, 312, 792, 822, 944, 996
MspA1I CMGCKG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 683
MvaI CCWGG 1 cut(s) 683
MwoI GCNNNNNNNGC 4 cut(s) 412, 506, 1036, 1045
NdeII GATC 3 cut(s) 223, 901, 1015
NlaIII CATG 4 cut(s) 533, 551, 724, 908
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 256
NsbI TGCGCA 3 cut(s) 213, 605, 764
NsiI ATGCAT 2 cut(s) 511, 726
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 3 cut(s) 18, 472, 1106
PleI GAGTC 2 cut(s) 137, 944
PpsI GAGTC 2 cut(s) 137, 944
Psp6I CCWGG 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 746
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 543
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PstNI CAGNNNCTG 2 cut(s) 473, 1036
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 5 cut(s) 192, 543, 572, 958, 1054
RsaNI GTAC 5 cut(s) 191, 542, 571, 957, 1053
SaqAI TTAA 6 cut(s) 12, 312, 792, 822, 944, 996
SatI GCNGC 3 cut(s) 17, 471, 1105
Sau3AI GATC 3 cut(s) 223, 901, 1015
SchI GAGTC 2 cut(s) 138, 944
ScrFI CCNGG 1 cut(s) 683
SfaNI GCATC 4 cut(s) 367, 518, 750, 774
Sse9I AATT 6 cut(s) 44, 100, 361, 558, 625, 1010
SsiI CCGC 2 cut(s) 435, 1112
SspI AATATT 1 cut(s) 738
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 3 cut(s) 10, 89, 1057
TaiI ACGT 1 cut(s) 41
TaqI TCGA 7 cut(s) 104, 147, 226, 446, 576, 729, 1001
TasI AATT 6 cut(s) 44, 100, 361, 558, 625, 1010
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 6 cut(s) 12, 312, 792, 822, 944, 996
Tru9I TTAA 6 cut(s) 12, 312, 792, 822, 944, 996
TscAI CASTG 2 cut(s) 221, 1092
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 3 cut(s) 16, 470, 1104
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 709
TspRI CASTG 2 cut(s) 221, 1092
XapI RAATTY 2 cut(s) 361, 1010
XspI CTAG 1 cut(s) 660
Zsp2I ATGCAT 2 cut(s) 511, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.