pycom10g21120

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015469
Physical Location & Seq
Reverse (-)
23839666 .. 23840705
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGCAATCAGAAGCTGCTGCCAGCACACGGAACCTTATCCAATTAAGTAATACGATCAAATCCAATGGCAAAGGGCCACGTCAGTTACTCGTGTGGCTGTGGCTCAAAAGGTTTATAACAATATTACGGGATGCAAAAGCTCATAAAATTATCAAAACAAAAGAAAGAGAGAAATTGCAATACAAAATTCAGAAAATGGCAGCTGCCACCGTGACCACTTCTCTCCCACAATTCAACGGCCTCAGACCCAAAATCTCATTCTCCAATGCGCAAAGCATGGCAGTTGTTCAGCCGATGAGGCGCAAGGGCCAAGGCGCTCTGGGAGCTCGCTGCGATTTCATCGGCTCACCTACCAATTTGATAATGGTGACCACAACAACCCTAATGTTGTTTGCCGGAAGATTCGGGTTGGCTCCGTCGGCAAACAGGAAATCAACGGCAGGATTGAAGCTTGAAACAAGGGACTCTGGGCTGCAGACCGGCGACCCAGCTGGGTTTACACTTGCTGATACATTGGCCTGTGGTTCTGTTGGTCACATTATTGGGGTTGGAGTTGTTCTTGGCCTTAAGAATATTGGTGCCCTATAA

Protein Analysis

196

Amino Acids

20.94

Weight (kDa)

10.89

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 118 - 193 1.3e-18 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 116
Acc16I TGCGCA 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 578
AcsI RAATTY 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 27
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 3 cut(s) 235, 448, 455
AjiI CACGTC 1 cut(s) 80
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 6 cut(s) 14, 140, 203, 326, 451, 491
AluI AGCT 6 cut(s) 14, 140, 203, 326, 451, 491
Alw21I GWGCWC 1 cut(s) 328
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 5 cut(s) 74, 238, 307, 516, 562
ApeKI GCWGC 6 cut(s) 14, 17, 200, 203, 330, 472
ApoI RAATTY 1 cut(s) 186
AspLEI GCGC 3 cut(s) 271, 303, 317
AspS9I GGNCC 2 cut(s) 74, 307
AsuHPI GGTGA 2 cut(s) 339, 379
BaeGI GKGCMC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 328
BauI CACGAG 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 5 cut(s) 4, 190, 212, 317, 459
BceAI ACGGC 2 cut(s) 253, 453
BfmI CTRYAG 1 cut(s) 473
BfoI RGCGCY 1 cut(s) 318
BfrI CTTAAG 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 298
BisI GCNGC 6 cut(s) 15, 18, 201, 204, 331, 473
BlsI GCNGC 6 cut(s) 16, 19, 202, 205, 332, 474
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 74, 307
BmiI GGNNCC 3 cut(s) 32, 414, 580
BmsI GCATC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 1 cut(s) 27
Bse118I RCCGGY 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 256
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 5 cut(s) 4, 190, 212, 317, 459
BseYI CCCAGC 2 cut(s) 487, 491
BshFI GGCC 5 cut(s) 76, 240, 309, 518, 564
BshNI GGYRCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 2 cut(s) 396, 480
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 5 cut(s) 76, 240, 309, 518, 564
Bsp1286I GDGCHC 2 cut(s) 328, 583
Bsp143I GATC 1 cut(s) 54
BspANI GGCC 5 cut(s) 76, 240, 309, 518, 564
BspCNI CTCAG 1 cut(s) 255
BspLI GGNNCC 3 cut(s) 32, 414, 580
BspMAI CTGCAG 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 578
BspTI CTTAAG 1 cut(s) 566
BsrFI RCCGGY 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 22, 328
BstDEI CTNAG 1 cut(s) 242
BstEII GGTNACC 1 cut(s) 367
BstF5I GGATG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 3 cut(s) 271, 303, 317
BstKTI GATC 1 cut(s) 57
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 298, 323, 419
BstPI GGTNACC 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 5 cut(s) 4, 190, 212, 317, 459
BsuRI GGCC 5 cut(s) 76, 240, 309, 518, 564
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 22, 328
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 3 cut(s) 271, 303, 317
Cfr10I RCCGGY 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 74, 307
CviAII CATG 1 cut(s) 277
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 326
Eco91I GGTNACC 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 1 cut(s) 280
FaiI YATR 4 cut(s) 116, 144, 278, 586
FaqI GGGAC 1 cut(s) 476
FatI CATG 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 15, 18, 201, 204, 331, 473
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 6 cut(s) 15, 18, 201, 204, 331, 473
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 3 cut(s) 270, 302, 316
GluI GCNGC 6 cut(s) 15, 18, 201, 204, 331, 473
GsaI CCCAGC 2 cut(s) 491, 495
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 5 cut(s) 76, 240, 309, 518, 564
HapII CCGG 2 cut(s) 396, 480
HhaI GCGC 3 cut(s) 271, 303, 317
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 3 cut(s) 269, 301, 315
HinP1I GCGC 3 cut(s) 269, 301, 315
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 2 cut(s) 402, 464
HpaII CCGG 2 cut(s) 396, 480
HphI GGTGA 2 cut(s) 339, 379
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 3 cut(s) 10, 192, 245
Hpy8I GTNNAC 1 cut(s) 498
Hpy99I CGWCG 1 cut(s) 421
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 4, 134, 178, 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 298, 323, 419
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 3 cut(s) 269, 301, 315
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 323, 418
Lsp1109I GCAGC 5 cut(s) 4, 190, 212, 317, 459
LweI GCATC 1 cut(s) 121
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 4 cut(s) 84, 211, 367, 533
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 1 cut(s) 411
MhlI GDGCHC 2 cut(s) 328, 583
MluCI AATT 6 cut(s) 41, 147, 173, 186, 230, 355
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 2 cut(s) 251, 291
MseI TTAA 2 cut(s) 44, 567
MspA1I CMGCKG 2 cut(s) 203, 491
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 2 cut(s) 396, 480
MwoI GCNNNNNNNGC 3 cut(s) 298, 323, 419
NdeII GATC 1 cut(s) 54
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 3 cut(s) 32, 414, 580
NmuCI GTSAC 3 cut(s) 211, 367, 533
NsbI TGCGCA 1 cut(s) 270
PfeI GAWTC 1 cut(s) 402
PkrI GCNGC 6 cut(s) 16, 19, 202, 205, 332, 474
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PsiI TTATAA 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 328
PspEI GGTNACC 1 cut(s) 367
PspFI CCCAGC 2 cut(s) 487, 491
PspN4I GGNNCC 3 cut(s) 32, 414, 580
PspPI GGNCC 2 cut(s) 74, 307
PstI CTGCAG 1 cut(s) 477
PstNI CAGNNNCTG 1 cut(s) 14
PvuII CAGCTG 2 cut(s) 203, 491
SacI GAGCTC 1 cut(s) 328
SaqAI TTAA 2 cut(s) 44, 567
SatI GCNGC 6 cut(s) 15, 18, 201, 204, 331, 473
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 74, 307
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 2 cut(s) 328, 583
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 473
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 6 cut(s) 41, 147, 173, 186, 230, 355
SspI AATATT 2 cut(s) 123, 574
SstI GAGCTC 1 cut(s) 328
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 82
TasI AATT 6 cut(s) 41, 147, 173, 186, 230, 355
TfiI GAWTC 1 cut(s) 402
Tru1I TTAA 2 cut(s) 44, 567
Tru9I TTAA 2 cut(s) 44, 567
TseFI GTSAC 3 cut(s) 211, 367, 533
TseI GCWGC 6 cut(s) 14, 17, 200, 203, 330, 472
Tsp45I GTSAC 3 cut(s) 211, 367, 533
TspDTI ATGAA 1 cut(s) 328
TspGWI ACGGA 2 cut(s) 43, 405
Vha464I CTTAAG 1 cut(s) 566
XapI RAATTY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.