pycom10g21540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010843
Physical Location & Seq
Forward (+)
24132329 .. 24132898
570 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGGCTGAGGCATCGCACGCGGTTTCTAACAAGTGGCTTTGTGAAAATTACGACGTTGTTAAACGACAAATCTTTCGCACATATCAATTCCTACAAACTCAATATCTCCACTTCTCTCTTAGTAATCTTACAAGATTATTGATCATATTCTGTATTTTCAAGGTGCTTAATTTAGAGAAACTGTATTATCTCACAGAGATCATGATGGTGAAGGAGGTGAGCTACATGCACAACTGGGGTGAAGTTGCACCGACCCCTTTCATATCTCATCAGAAATCTTCGAAATGTCCAGAGCTGGAGACAATTGTTGAAGAAGCGGGACCAGTTGGACCTCAGATTAACATTGAAATTCTACCGTTTTCAAAGAGGGCGTTGTGTCTGGTTCCAGTGTTTGTTTCTTGTATTTCTTATTTCTTGTTGTACAGATATGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.98

Weight (kDa)

6.89

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 20, 317
AcsI RAATTY 1 cut(s) 348
AfaI GTAC 1 cut(s) 422
AgsI TTSAA 4 cut(s) 160, 311, 347, 363
AluBI AGCT 2 cut(s) 222, 295
AluI AGCT 2 cut(s) 222, 295
Alw26I GTCTC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 348
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspS9I GGNCC 2 cut(s) 320, 329
AsuHPI GGTGA 3 cut(s) 220, 229, 251
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 2 cut(s) 320, 329
BbvCI CCTCAGC 1 cut(s) 6
BccI CCATC 1 cut(s) 199
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 293
Bme18I GGWCC 2 cut(s) 320, 329
BmgT120I GGNCC 2 cut(s) 320, 329
BmiI GGNNCC 2 cut(s) 321, 384
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 317
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 281
Bse1I ACTGG 3 cut(s) 239, 323, 386
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 3 cut(s) 239, 323, 386
Bsh1236I CGCG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 281
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 141, 198
BspACI CCGC 2 cut(s) 20, 317
BspCNI CTCAG 1 cut(s) 346
BspFNI CGCG 1 cut(s) 20
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 321, 384
BspT104I TTCGAA 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 3 cut(s) 239, 323, 386
BssMI GATC 2 cut(s) 141, 198
Bst4CI ACNGT 2 cut(s) 183, 357
BstAUI TGTACA 1 cut(s) 420
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 3 cut(s) 6, 119, 333
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 2 cut(s) 144, 201
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 2 cut(s) 141, 198
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 229
BstUI CGCG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 18
CciI TCATGA 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 320, 329
Csp6I GTAC 1 cut(s) 421
CviAII CATG 2 cut(s) 202, 226
CviJI RGCY 4 cut(s) 5, 37, 222, 295
CviKI_1 RGCY 4 cut(s) 5, 37, 222, 295
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 3 cut(s) 6, 119, 333
DpnI GATC 2 cut(s) 143, 200
DpnII GATC 2 cut(s) 141, 198
Eco47I GGWCC 2 cut(s) 320, 329
FaeI CATG 2 cut(s) 205, 229
FaiI YATR 6 cut(s) 82, 146, 203, 227, 263, 429
FaqI GGGAC 1 cut(s) 333
FatI CATG 2 cut(s) 201, 225
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 317
Hin1II CATG 2 cut(s) 205, 229
HphI GGTGA 3 cut(s) 220, 229, 251
Hpy188I TCNGA 2 cut(s) 273, 336
Hpy188III TCNNGA 2 cut(s) 202, 290
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 183, 357
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 229, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 6, 119, 333
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 2 cut(s) 205, 229
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 198
LpnPI CCDG 6 cut(s) 220, 281, 303, 336, 365, 399
LweI GCATC 1 cut(s) 20
MaeII ACGT 1 cut(s) 54
MalI GATC 2 cut(s) 143, 200
MboI GATC 2 cut(s) 141, 198
MboII GAAGA 2 cut(s) 270, 323
MfeI CAATTG 1 cut(s) 303
MluCI AATT 5 cut(s) 46, 86, 169, 303, 348
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 3 cut(s) 208, 342, 360
MseI TTAA 3 cut(s) 60, 168, 339
MslI CAYNNNNRTG 1 cut(s) 206
MunI CAATTG 1 cut(s) 303
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 2 cut(s) 141, 198
NlaIII CATG 2 cut(s) 205, 229
NlaIV GGNNCC 2 cut(s) 321, 384
NspI RCATGY 1 cut(s) 229
NspV TTCGAA 1 cut(s) 281
PagI TCATGA 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 321, 384
PspPI GGNCC 2 cut(s) 320, 329
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 3 cut(s) 60, 168, 339
Sau3AI GATC 2 cut(s) 141, 198
Sau96I GGNCC 2 cut(s) 320, 329
SetI ASST 6 cut(s) 57, 165, 219, 224, 297, 334
SfaNI GCATC 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 2 cut(s) 320, 329
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 5 cut(s) 46, 86, 169, 303, 348
SsiI CCGC 2 cut(s) 20, 317
TaaI ACNGT 2 cut(s) 183, 357
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 281
TasI AATT 5 cut(s) 46, 86, 169, 303, 348
TatI WGTACW 1 cut(s) 420
Tru1I TTAA 3 cut(s) 60, 168, 339
Tru9I TTAA 3 cut(s) 60, 168, 339
TscAI CASTG 1 cut(s) 393
TspDTI ATGAA 1 cut(s) 250
TspRI CASTG 1 cut(s) 393
VpaK11BI GGWCC 2 cut(s) 320, 329
XapI RAATTY 1 cut(s) 348
XceI RCATGY 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.