pycom10g25540

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011265
Physical Location & Seq
Forward (+)
27139207 .. 27141337
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGGCGGGCAAGGCTGTAGAGGAAGCGATTCCGGCCGTAAATCCCCGAGGTTCTCACATAAAGAAGAGGGCTCTCAAAAACAAAGCTCTCGCCGTCACGTTCAATGAGAAAGACCTCAGCGATTTCGTGTCTGGGTTTCACAAGAGGAAGAAGAAGAGGAGAAAGGAAGCTGAGCAGAAGCAAGGGGAGGCGCTGCGGCGGAAGCGTCTCGAGCTGCGCAAAAAGAGGAGACTAGAAAAGGAACTGGTTCTTAATGGTGGGATTACCCCAGCCACTGATACAGCAGCTGATGAGGTTGATGAGGGTGATGAGGACCACGAGGATGATGAAGATAGTGAGCCATCTGTACCAATTTCTGGTACAACGACTTATGACAACGGCGAAATGAAAGTCGTTGTGACAACAAGTGAGATTTCTCGAGAGGAGGAAAGTGATCCAGATGAAAAGCCAGAGGCAGCAGTACCGGAATCAGTCGGAGCTTCTAAAAAACACAAGATACCAGTGAAAAAGACCAAACCTCTGAAAAAAGCTGCAAAGCGTAAATCCCGGCCCAAGCTACAACGGAAAAGAGATAAAAGGAAGGGAATAAAACCGACCAAGAACAAGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

204

Amino Acids

22.86

Weight (kDa)

9.85

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop25 PF09805 21 - 155 4.9e-17 Nucleolar protein 12 (25kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 356
AciI CCGC 3 cut(s) 5, 196, 199
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 33
AfaI GTAC 3 cut(s) 348, 361, 462
AfeI AGCGCT 1 cut(s) 608
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 103
AluBI AGCT 7 cut(s) 86, 170, 214, 287, 479, 530, 556
AluI AGCT 7 cut(s) 86, 170, 214, 287, 479, 530, 556
Alw26I GTCTC 2 cut(s) 212, 223
AlwI GGATC 1 cut(s) 428
AlwNI CAGNNNCTG 2 cut(s) 275, 287
Ama87I CYCGRG 3 cut(s) 45, 209, 417
Aor51HI AGCGCT 1 cut(s) 608
AoxI GGCC 2 cut(s) 33, 548
ApeKI GCWGC 5 cut(s) 193, 214, 284, 455, 530
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
Asp700I GAANNNNTTC 2 cut(s) 27, 246
AspLEI GCGC 3 cut(s) 193, 219, 609
AspS9I GGNCC 2 cut(s) 313, 549
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 3 cut(s) 45, 209, 417
AvaII GGWCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 73
BauI CACGAG 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 116
BbvI GCAGC 5 cut(s) 180, 201, 296, 467, 517
BccI CCATC 1 cut(s) 349
BceAI ACGGC 3 cut(s) 20, 77, 394
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 212, 223
BfaI CTAG 2 cut(s) 233, 610
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 194, 610
BisI GCNGC 6 cut(s) 194, 197, 215, 285, 456, 531
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 6 cut(s) 195, 198, 216, 286, 457, 532
Bme1390I CCNGG 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 3 cut(s) 45, 209, 417
BmgT120I GGNCC 2 cut(s) 313, 549
BmrFI CCNGG 1 cut(s) 547
Bpu10I CCTNAGC 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 547
BsaJI CCNNGG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 249, 500
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 130, 162
BseNI ACTGG 2 cut(s) 249, 500
BseRI GAGGAG 3 cut(s) 172, 241, 437
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 5 cut(s) 180, 201, 296, 467, 517
BseYI CCCAGC 1 cut(s) 268
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 35, 550
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 3 cut(s) 45, 209, 417
BsiSI CCGG 3 cut(s) 32, 464, 547
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 2 cut(s) 212, 223
BsmBI CGTCTC 1 cut(s) 212
BsnI GGCC 2 cut(s) 35, 550
BsoBI CYCGRG 3 cut(s) 45, 209, 417
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 3 cut(s) 5, 196, 199
BspANI GGCC 2 cut(s) 35, 550
BspCNI CTCAG 2 cut(s) 129, 163
BspPI GGATC 1 cut(s) 428
BsrI ACTGG 2 cut(s) 249, 500
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 1 cut(s) 433
BssSI CACGAG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 317
Bst6I CTCTTC 2 cut(s) 59, 149
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 116, 171
BstF5I GGATG 1 cut(s) 328
BstH2I RGCGCY 2 cut(s) 194, 610
BstHHI GCGC 3 cut(s) 193, 219, 609
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 2 cut(s) 212, 223
BstMBI GATC 1 cut(s) 433
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 11, 32, 202, 211
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 5 cut(s) 180, 201, 296, 467, 517
BstZI CGGCCG 1 cut(s) 33
BsuRI GGCC 2 cut(s) 35, 550
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 273, 507
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 2 cut(s) 275, 287
CfoI GCGC 3 cut(s) 193, 219, 609
Cfr13I GGNCC 2 cut(s) 313, 549
CseI GACGC 1 cut(s) 194
Csp6I GTAC 3 cut(s) 347, 360, 461
CviQI GTAC 3 cut(s) 347, 360, 461
DdeI CTNAG 2 cut(s) 116, 171
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 33
EagI CGGCCG 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 59, 149
EarI CTCTTC 2 cut(s) 59, 149
EciI GGCGGA 1 cut(s) 214
EclXI CGGCCG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 313
Eco47III AGCGCT 1 cut(s) 608
Eco52I CGGCCG 1 cut(s) 33
Eco88I CYCGRG 3 cut(s) 45, 209, 417
EcoT38I GRGCYC 1 cut(s) 73
Esp3I CGTCTC 1 cut(s) 212
FaiI YATR 2 cut(s) 59, 372
Fnu4HI GCNGC 6 cut(s) 194, 197, 215, 285, 456, 531
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 6 cut(s) 194, 197, 215, 285, 456, 531
FspBI CTAG 2 cut(s) 233, 610
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 3 cut(s) 192, 218, 608
GluI GCNGC 6 cut(s) 194, 197, 215, 285, 456, 531
GsaI CCCAGC 1 cut(s) 272
HaeII RGCGCY 2 cut(s) 194, 610
HaeIII GGCC 2 cut(s) 35, 550
HapII CCGG 3 cut(s) 32, 464, 547
HgaI GACGC 1 cut(s) 194
HhaI GCGC 3 cut(s) 193, 219, 609
Hin6I GCGC 3 cut(s) 191, 217, 607
HinP1I GCGC 3 cut(s) 191, 217, 607
HinfI GANTC 2 cut(s) 28, 467
HpaII CCGG 3 cut(s) 32, 464, 547
HphI GGTGA 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 476, 522
Hpy188III TCNNGA 4 cut(s) 209, 417, 419, 437
HpyAV CCTTC 1 cut(s) 574
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 1 cut(s) 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 32, 202, 211
HpyF3I CTNAG 2 cut(s) 116, 171
HpySE526I ACGT 1 cut(s) 98
HspAI GCGC 3 cut(s) 191, 217, 607
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 476
Lsp1109I GCAGC 5 cut(s) 180, 201, 296, 467, 517
MaeI CTAG 2 cut(s) 233, 610
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 94, 397
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 5 cut(s) 76, 160, 163, 166, 341
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 1 cut(s) 351
MmeI TCCRAC 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 27, 246
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 3 cut(s) 32, 464, 547
MspR9I CCNGG 1 cut(s) 547
MwoI GCNNNNNNNGC 4 cut(s) 11, 32, 202, 211
NciI CCSGG 1 cut(s) 547
NdeII GATC 1 cut(s) 433
NmuCI GTSAC 2 cut(s) 94, 397
NsbI TGCGCA 1 cut(s) 218
PaeR7I CTCGAG 2 cut(s) 209, 417
PdmI GAANNNNTTC 2 cut(s) 27, 246
PfeI GAWTC 2 cut(s) 28, 467
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 6 cut(s) 195, 198, 216, 286, 457, 532
PspFI CCCAGC 1 cut(s) 268
PspPI GGNCC 2 cut(s) 313, 549
PstNI CAGNNNCTG 2 cut(s) 275, 287
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 3 cut(s) 348, 361, 462
RsaNI GTAC 3 cut(s) 347, 360, 461
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 6 cut(s) 194, 197, 215, 285, 456, 531
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 2 cut(s) 313, 549
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 15
Sfr274I CTCGAG 2 cut(s) 209, 417
SinI GGWCC 1 cut(s) 313
SlaI CTCGAG 2 cut(s) 209, 417
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 209, 417
SmoI CTYRAG 2 cut(s) 209, 417
Sse9I AATT 1 cut(s) 351
SsiI CCGC 3 cut(s) 5, 196, 199
SspMI CTAG 2 cut(s) 233, 610
StyD4I CCNGG 1 cut(s) 545
TaiI ACGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 210, 418
TasI AATT 1 cut(s) 351
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 2 cut(s) 28, 467
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 2 cut(s) 280, 507
TseFI GTSAC 2 cut(s) 94, 397
TseI GCWGC 5 cut(s) 193, 214, 284, 455, 530
Tsp45I GTSAC 2 cut(s) 94, 397
TspDTI ATGAA 3 cut(s) 342, 401, 456
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 280, 507
Van91I CCANNNNNTGG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 313
XhoI CTCGAG 2 cut(s) 209, 417
XmnI GAANNNNTTC 2 cut(s) 27, 246
XspI CTAG 2 cut(s) 233, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.