pycom10g27230

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003232
Physical Location & Seq
Forward (+)
28257934 .. 28263272
5339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2835 bp
ATGGAGGTTTCTATACAAGCACAGAGGCCACTCAGTTGCAGAGGAATTTTCCAACAGAGAGGAAGCAACTTAAAGCTCAAACCTTTTACTGGATTCCCTCCCCATGGAAGATATTCTTCATGGTTCAAAGGAAACCTGGCAAATGGGGTTTCATACCAAATTACTGCCCGTTCAGCCGATTTTTCAAGGAGGAGAGAAAGAAAAGTGTCTCCGGGTTCAAAGGGATTAGTGCCGAAAAACCCAGTAGGAACTAGCGGCCAGAAGACGAATCAAAGGAATATTGGGGACAAAAAGGGTATTACATCTTTGACATCCAGTGAACTTGATGGGAAAAATAAGAAAACACTTGAATCAAGAGTTGACACCAGAAGAGAATTGGTAGTTGAACCTTCAGAAGAGAGCGATGTTGAGGAAAAAAGAATTGATGAAACGAGTACTAAGGTTGAGGAATCATCTTCATTTAGCAAGCCATCAAGTGGGGGAATTCAAGGGATAAAAAATGGGAGTGTAGGTAAGGTCCTTGATGACTTGGCAGACACTGATAAAGGCATTGATGCTAGTGAAAAGTTAACGAATGAAGTTTCTTTAAAGTTAAAGTTGGAGAGGGAAGAAAAATTGCGTAAAGAGGAAATTGCGAGGCTTGCTGAGGAGAACTTCTCAAGAGGGAACAAAATCTTTGTCTATCCTCAGGTGGTGAAACCTGATCAAGACATAGAAGTGTTCCTTAATAGAAGTATATCAACCCTAAGCAATGAGCCAGAGGTTCAGATGATGGGTGCCTTTAACGACTGGCGTTGGAAATCTTTTAACTTCAGGTTGAACAAAACACAACTCAAAGGGGATTGGTGGTCTTGCCAGTTTCATGTTCCTAAAGAATCATATAAGATAGACTTTGTGTTCTTCAATGGGCAAAATGTTTATGACAATAATGATGCGAAAAATTTCTGGATAACTGTGGAAGGTGGAATGGACCTCTTGGCATTTGAAGATTTCTTACTTGAGGAGAAGCGTAAAGAACAAGAGAAACTTGCAAAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATCGAAGCAGAGAAGGCAGCAAGTGAAGCTGACAGAGCACAGGCAAAGGCAGAGATTGTAAAGAGACAACAAATGGTGCAAGAGTTGATCAAAAAGGCTGTAAGGTCTGTAGAAGACGTTTGGCACATAGAGCCTAGAGAGTTTAAAGGTGAGGACTTGGTGAAGTTATACTATAACCGAAGCTCGGGTCCTCTTGCTCATGCTAAGGAACTATGGATTCATGGGGGGCATAATGGTTGGACAGATGGATTGTCAATTGTTGAAAGACTTGTTAGCTCTGAGAGAAAGGATGGTGACTGGTGGTATGCCAATGTTGTTGTACCTGAACAAGCTGTTGTCTTGGATTGGGTTTTTGCTGATGGACCACCTCAGAATGCCGTTCTATATGATAACAACCACCGTCATGATTTCCATGCTATAGTTCCAAAATCCATTCCTGAGGAACTAGATTGGGTTGAGGAAGAACACCAGATATATAAGAAGCTCCAGGAGGAGAGGAGATTAAAGGAGGAGGCAATCCGTGCCAAGGTCGAAAGAACAGCTCGTATGAAAGCAGAAATGAAGGAAAGAACTTTGAAAAGGTTTTTGCTGTCTCAGAAGCATATAGTATACACTGAGCCTCTTGATGTTCAAGCTGGAAACAAGGCGACAGTTTTCTACAATCCTGCCAACACAGTTCTGAATGGAAAGCCTGAGGTTTGGTTCAGACGATCATTTAACCGTTGGACCCACCGCAAGGGTCCATTGCAACCACAGAAGATGTTACCTGCAGAGAATGGTTCTCGTGTCAAAACAATTGTTCCAGTCCCACTCGATGCATACATGATGGACTTTGTTTTCTCTGAGACGGAAGATGGTGGACTTTTTGACAACAATAATGGCATGGATTACCACATTCCGGTGTTTGGAGGAGTTGCGAAGGAGCCACCAATGAACATTGTACATATTGCTGTTGAAATGGCCCCCATTGCAAAGGTTGGAGGCCTTGGTGACGTTGTTACCAGTCTCTCACGAGCTGTGCAAGACTTAAATCACCACGTTGACATCATTCTTCCAAAATATGATTGTTTGAACTTTAGCAATGTGAAGGAGTTTCAGTATAACAGAAGCTTCTCTTGGGGTGGAACTGAAATAAAAGTTTGGTTTGGGAAAGTGGAAGGCGTTTCAGTGTACTTTTTGGAACCTCAAAACAGATTCTTTGATGCAGGTTGCGTATATGGCTGTAAAAATGATGCAGAGAGATTTGGTTTCTTTTGCCATGCTGCGCTAGAATTTCTACTTCAAAGTGGATTTCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCACCTGTTGCCTGGTTATTTAAAGATCATTACATGCATTATGGTCTCAGTAAAGCTCGGGTTGTCTTCACAATTCATAACCTTGAGTTTGGAACGCACTTCATTGGTAAAGCTGTGGCGTACTCAGACAAATCCACAACAGTATCCAACACTTACGCTAAGGAGGTATCTGGAAATCCTGCAATTGCTTCTCATCTTTACAAGTTTCATGGTATAATAAATGGAATTGACCAAGATATATGGGATCCATACGATGATAAGTTCATTCCTATATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGATCGATCTCCCTGTAATAGGTATTATTACGCGCTTAACTCGCCAGAAAGGAATCCATCTCATCAAGCATGCCATTAG

Protein Analysis

945

Amino Acids

107.94

Weight (kDa)

6.76

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 239 - 298 1.6e-09 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 413 - 475 3.5e-15 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 576 - 637 6e-10 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 671 - 859 1.6e-49 Starch synthase catalytic domain
Glyco_transf_4 PF13439 685 - 858 4.7e-09 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1852, 2273
AccB1I GGYRCC 1 cut(s) 776
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 1 cut(s) 1686
AccII CGCG 1 cut(s) 2789
AciI CCGC 2 cut(s) 255, 1810
AclWI GGATC 2 cut(s) 2645, 2658
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 4 cut(s) 45, 483, 940, 2348
AcuI CTGAAG 2 cut(s) 375, 796
AfaI GTAC 5 cut(s) 436, 1398, 2019, 2249, 2528
AfiI CCNNNNNNNGG 9 cut(s) 89, 104, 476, 1261, 1603, 1813, 1975, 1982, 2775
AjnI CCWGG 3 cut(s) 135, 1563, 2417
AjuI GAANNNNNNNTTGG 2 cut(s) 2176, 2208
Alw21I GWGCWC 2 cut(s) 1117, 2410
Alw26I GTCTC 6 cut(s) 213, 1135, 1674, 1916, 2087, 2456
Alw44I GTGCAC 1 cut(s) 2406
AlwI GGATC 2 cut(s) 2645, 2658
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 2 cut(s) 1261, 2463
AoxI GGCC 4 cut(s) 26, 256, 2037, 2059
ApaLI GTGCAC 1 cut(s) 2406
ApeKI GCWGC 3 cut(s) 1094, 2339, 2717
ApoI RAATTY 4 cut(s) 45, 483, 940, 2348
ArsI GACNNNNNNTTYG 6 cut(s) 663, 695, 1249, 1281, 2731, 2763
Asp700I GAANNNNTTC 2 cut(s) 112, 941
AspLEI GCGC 2 cut(s) 2344, 2791
AspS9I GGNCC 7 cut(s) 517, 970, 1265, 1439, 1803, 1817, 2038
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 6 cut(s) 706, 1238, 1249, 1382, 2078, 2102
AvaI CYCGRG 2 cut(s) 1261, 2463
AvaII GGWCC 6 cut(s) 517, 970, 1265, 1439, 1803, 1817
AxyI CCTNAGG 3 cut(s) 687, 1515, 1770
BaeGI GKGCMC 1 cut(s) 2410
BaeI ACNNNNGTAYC 2 cut(s) 2532, 2565
BamHI GGATCC 1 cut(s) 2650
BanI GGYRCC 1 cut(s) 776
BarI GAAGNNNNNNTAC 2 cut(s) 978, 1010
BauI CACGAG 2 cut(s) 1860, 2088
BbsI GAAGAC 3 cut(s) 269, 1197, 2464
Bbv12I GWGCWC 2 cut(s) 1117, 2410
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 1106, 2326, 2704
BccI CCATC 9 cut(s) 320, 478, 766, 1316, 1361, 1430, 1897, 1925, 2821
BceAI ACGGC 1 cut(s) 1439
BciT130I CCWGG 3 cut(s) 137, 1565, 2419
BciVI GTATCC 1 cut(s) 2560
BclI TGATCA 2 cut(s) 703, 1164
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 6 cut(s) 213, 1135, 1674, 1916, 2087, 2456
BfaI CTAG 6 cut(s) 252, 558, 1212, 1523, 2345, 2403
BfmI CTRYAG 3 cut(s) 1185, 1494, 1845
BfuAI ACCTGC 2 cut(s) 1852, 2273
BfuI GTATCC 1 cut(s) 2560
BisI GCNGC 4 cut(s) 256, 1095, 2340, 2718
BlsI GCNGC 4 cut(s) 257, 1096, 2341, 2719
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 4 cut(s) 137, 213, 1565, 2419
Bme18I GGWCC 6 cut(s) 517, 970, 1265, 1439, 1803, 1817
BmeT110I CYCGRG 2 cut(s) 1261, 2463
BmgT120I GGNCC 7 cut(s) 517, 970, 1265, 1439, 1803, 1817, 2038
BmiI GGNNCC 8 cut(s) 778, 1266, 1805, 1818, 2001, 2040, 2259, 2652
BmrFI CCNGG 4 cut(s) 137, 213, 1565, 2419
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 5 cut(s) 544, 922, 1882, 2269, 2299
BmuI ACTGGG 1 cut(s) 236
BpiI GAAGAC 3 cut(s) 269, 1197, 2464
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 1 cut(s) 1547
Bpu10I CCTNAGC 4 cut(s) 645, 746, 1281, 2565
BpuEI CTTGAG 3 cut(s) 643, 1019, 2510
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 2761
BsaI GGTCTC 1 cut(s) 2456
BsaJI CCNNGG 4 cut(s) 103, 1602, 2062, 2721
BsaWI WCCGGW 1 cut(s) 1975
BsaXI ACNNNNNCTCC 4 cut(s) 496, 526, 2159, 2189
Bsc4I CCNNNNNNNGG 9 cut(s) 89, 104, 476, 1261, 1603, 1813, 1975, 1982, 2775
Bse1I ACTGG 8 cut(s) 94, 242, 315, 794, 856, 1379, 1880, 2079
Bse21I CCTNAGG 3 cut(s) 687, 1515, 1770
Bse3DI GCAATG 5 cut(s) 757, 1820, 2043, 2164, 2386
BseBI CCWGG 3 cut(s) 137, 1565, 2419
BseCI ATCGAT 1 cut(s) 2761
BseDI CCNNGG 4 cut(s) 103, 1602, 2062, 2721
BseGI GGATG 3 cut(s) 311, 1372, 2371
BseLI CCNNNNNNNGG 9 cut(s) 89, 104, 476, 1261, 1603, 1813, 1975, 1982, 2775
BseMI GCAATG 5 cut(s) 757, 1820, 2043, 2164, 2386
BseNI ACTGG 8 cut(s) 94, 242, 315, 794, 856, 1379, 1880, 2079
BseRI GAGGAG 7 cut(s) 205, 662, 1016, 1583, 1588, 1601, 2001
BseSI GKGCMC 1 cut(s) 2410
BseXI GCAGC 3 cut(s) 1106, 2326, 2704
Bsh1236I CGCG 1 cut(s) 2789
BshFI GGCC 4 cut(s) 28, 258, 2039, 2061
BshNI GGYRCC 1 cut(s) 776
BshVI ATCGAT 1 cut(s) 2761
BsiHKAI GWGCWC 2 cut(s) 1117, 2410
BsiHKCI CYCGRG 2 cut(s) 1261, 2463
BsiSI CCGG 2 cut(s) 212, 1976
BslFI GGGAC 2 cut(s) 299, 1868
BslI CCNNNNNNNGG 9 cut(s) 89, 104, 476, 1261, 1603, 1813, 1975, 1982, 2775
BsmAI GTCTC 6 cut(s) 213, 1135, 1674, 1916, 2087, 2456
BsmBI CGTCTC 1 cut(s) 1916
BsmFI GGGAC 2 cut(s) 299, 1868
BsmI GAATGC 1 cut(s) 1456
BsnI GGCC 4 cut(s) 28, 258, 2039, 2061
Bso31I GGTCTC 1 cut(s) 2456
BsoBI CYCGRG 2 cut(s) 1261, 2463
Bsp1286I GDGCHC 2 cut(s) 1117, 2410
Bsp1407I TGTACA 1 cut(s) 2017
Bsp143I GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 2 cut(s) 255, 1810
BspANI GGCC 4 cut(s) 28, 258, 2039, 2061
BspDI ATCGAT 1 cut(s) 2761
BspFNI CGCG 1 cut(s) 2789
BspHI TCATGA 1 cut(s) 1480
BspLI GGNNCC 8 cut(s) 778, 1266, 1805, 1818, 2001, 2040, 2259, 2652
BspMAI CTGCAG 1 cut(s) 1849
BspMI ACCTGC 2 cut(s) 1852, 2273
BspPI GGATC 2 cut(s) 2645, 2658
BspQI GCTCTTC 1 cut(s) 1059
BspT107I GGYRCC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 2456
BsrDI GCAATG 5 cut(s) 757, 1820, 2043, 2164, 2386
BsrGI TGTACA 1 cut(s) 2017
BsrI ACTGG 8 cut(s) 94, 242, 315, 794, 856, 1379, 1880, 2079
BssECI CCNNGG 4 cut(s) 103, 1602, 2062, 2721
BssMI GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
BssNAI GTATAC 1 cut(s) 1687
BssSI CACGAG 2 cut(s) 1860, 2088
BssT1I CCWWGG 4 cut(s) 103, 1602, 2062, 2721
Bst1107I GTATAC 1 cut(s) 1687
Bst2BI CACGAG 2 cut(s) 1860, 2088
Bst2UI CCWGG 3 cut(s) 137, 1565, 2419
Bst4CI ACNGT 6 cut(s) 955, 1478, 1729, 1753, 1799, 2548
Bst6I CTCTTC 3 cut(s) 364, 390, 1059
BstAPI GCANNNNNTGC 2 cut(s) 1598, 2414
BstAUI TGTACA 1 cut(s) 2017
BstC8I GCNNGC 5 cut(s) 467, 642, 1041, 1059, 2827
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 2773
BstF5I GGATG 3 cut(s) 311, 1372, 2371
BstFNI CGCG 1 cut(s) 2789
BstHHI GCGC 2 cut(s) 2344, 2791
BstKTI GATC 7 cut(s) 706, 1167, 1790, 2434, 2653, 2761, 2765
BstMAI GTCTC 6 cut(s) 213, 1135, 1674, 1916, 2087, 2456
BstMBI GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
BstNI CCWGG 3 cut(s) 137, 1565, 2419
BstNSI RCATGY 2 cut(s) 2443, 2829
BstSCI CCNGG 4 cut(s) 135, 211, 1563, 2417
BstSFI CTRYAG 3 cut(s) 1185, 1494, 1845
BstSLI GKGCMC 1 cut(s) 2410
BstUI CGCG 1 cut(s) 2789
BstV1I GCAGC 3 cut(s) 1106, 2326, 2704
BstV2I GAAGAC 3 cut(s) 269, 1197, 2464
BstX2I RGATCY 1 cut(s) 2650
BstYI RGATCY 1 cut(s) 2650
BstZ17I GTATAC 1 cut(s) 1687
Bsu15I ATCGAT 1 cut(s) 2761
Bsu36I CCTNAGG 3 cut(s) 687, 1515, 1770
BsuI GTATCC 1 cut(s) 2560
BsuRI GGCC 4 cut(s) 28, 258, 2039, 2061
BsuTUI ATCGAT 1 cut(s) 2761
BtgI CCRYGG 1 cut(s) 103
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 311, 1372, 2371
BtsIMutI CAGTG 4 cut(s) 322, 537, 1689, 2250
BveI ACCTGC 2 cut(s) 1852, 2273
Cac8I GCNNGC 5 cut(s) 467, 642, 1041, 1059, 2827
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 1480
CfoI GCGC 2 cut(s) 2344, 2791
Cfr13I GGNCC 7 cut(s) 517, 970, 1265, 1439, 1803, 1817, 2038
ClaI ATCGAT 1 cut(s) 2761
Csp6I GTAC 5 cut(s) 435, 1397, 2018, 2248, 2527
CviQI GTAC 5 cut(s) 435, 1397, 2018, 2248, 2527
DpnI GATC 7 cut(s) 705, 1166, 1789, 2433, 2652, 2760, 2764
DpnII GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
DraI TTTAAA 3 cut(s) 588, 1222, 2428
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 3 cut(s) 364, 390, 1059
EarI CTCTTC 3 cut(s) 364, 390, 1059
Eco130I CCWWGG 4 cut(s) 103, 1602, 2062, 2721
Eco147I AGGCCT 1 cut(s) 2061
Eco31I GGTCTC 1 cut(s) 2456
Eco32I GATATC 1 cut(s) 2380
Eco47I GGWCC 6 cut(s) 517, 970, 1265, 1439, 1803, 1817
Eco57I CTGAAG 2 cut(s) 375, 796
Eco81I CCTNAGG 3 cut(s) 687, 1515, 1770
Eco88I CYCGRG 2 cut(s) 1261, 2463
EcoNI CCTNNNNNAGG 1 cut(s) 2773
EcoO109I RGGNCCY 2 cut(s) 517, 1265
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 3 cut(s) 135, 1563, 2417
EcoRV GATATC 1 cut(s) 2380
EcoT14I CCWWGG 4 cut(s) 103, 1602, 2062, 2721
EcoT22I ATGCAT 2 cut(s) 1897, 2445
ErhI CCWWGG 4 cut(s) 103, 1602, 2062, 2721
Esp3I CGTCTC 1 cut(s) 1916
FaqI GGGAC 2 cut(s) 299, 1868
FbaI TGATCA 2 cut(s) 703, 1164
FblI GTMKAC 1 cut(s) 1686
Fnu4HI GCNGC 4 cut(s) 256, 1095, 2340, 2718
FokI GGATG 3 cut(s) 298, 1379, 2358
Fsp4HI GCNGC 4 cut(s) 256, 1095, 2340, 2718
FspBI CTAG 6 cut(s) 252, 558, 1212, 1523, 2345, 2403
GlaI GCGC 2 cut(s) 2343, 2790
GluI GCNGC 4 cut(s) 256, 1095, 2340, 2718
GsuI CTGGAG 1 cut(s) 1547
HaeIII GGCC 4 cut(s) 28, 258, 2039, 2061
HapII CCGG 2 cut(s) 212, 1976
HhaI GCGC 2 cut(s) 2344, 2791
Hin6I GCGC 2 cut(s) 2342, 2789
HinP1I GCGC 2 cut(s) 2342, 2789
HincII GTYRAC 3 cut(s) 361, 570, 2119
HindII GTYRAC 3 cut(s) 361, 570, 2119
HindIII AAGCTT 1 cut(s) 2185
HinfI GANTC 9 cut(s) 93, 268, 350, 449, 875, 1077, 1294, 2271, 2809
HpaI GTTAAC 1 cut(s) 570
HpaII CCGG 2 cut(s) 212, 1976
HphI GGTGA 6 cut(s) 706, 1238, 1249, 1382, 2078, 2102
Hpy166II GTNNAC 8 cut(s) 320, 361, 570, 1687, 1937, 2119, 2248, 2408
Hpy8I GTNNAC 8 cut(s) 320, 361, 570, 1687, 1937, 2119, 2248, 2408
HpyAV CCTTC 8 cut(s) 399, 953, 1084, 1633, 1990, 2158, 2228, 2699
HpyCH4III ACNGT 6 cut(s) 955, 1478, 1729, 1753, 1799, 2548
HpyCH4IV ACGT 3 cut(s) 1194, 2070, 2115
HpySE526I ACGT 3 cut(s) 1194, 2070, 2115
HspAI GCGC 2 cut(s) 2342, 2789
Ksp22I TGATCA 2 cut(s) 703, 1164
KspAI GTTAAC 1 cut(s) 570
Kzo9I GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
LguI GCTCTTC 1 cut(s) 1059
LmnI GCTCC 3 cut(s) 1036, 1566, 1999
Lsp1109I GCAGC 3 cut(s) 1106, 2326, 2704
LweI GCATC 5 cut(s) 544, 922, 1882, 2269, 2299
MaeI CTAG 6 cut(s) 252, 558, 1212, 1523, 2345, 2403
MaeII ACGT 3 cut(s) 1194, 2070, 2115
MaeIII GTNAC 4 cut(s) 1370, 1839, 2066, 2074
MalI GATC 7 cut(s) 705, 1166, 1789, 2433, 2652, 2760, 2764
MboI GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
MfeI CAATTG 3 cut(s) 1332, 1872, 2589
MflI RGATCY 1 cut(s) 2650
MhlI GDGCHC 2 cut(s) 1117, 2410
MmeI TCCRAC 7 cut(s) 76, 579, 776, 1295, 1781, 2035, 2577
Mph1103I ATGCAT 2 cut(s) 1897, 2445
MroXI GAANNNNTTC 2 cut(s) 112, 941
MslI CAYNNNNRTG 4 cut(s) 716, 1479, 1976, 2391
MspI CCGG 2 cut(s) 212, 1976
MspR9I CCNGG 4 cut(s) 137, 213, 1565, 2419
MunI CAATTG 3 cut(s) 1332, 1872, 2589
Mva1269I GAATGC 1 cut(s) 1456
MvaI CCWGG 3 cut(s) 137, 1565, 2419
MvnI CGCG 1 cut(s) 2789
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 103
NdeII GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
NlaIV GGNNCC 8 cut(s) 778, 1266, 1805, 1818, 2001, 2040, 2259, 2652
NmuCI GTSAC 2 cut(s) 1370, 2066
NsiI ATGCAT 2 cut(s) 1897, 2445
NspI RCATGY 2 cut(s) 2443, 2829
PaeI GCATGC 1 cut(s) 2829
PagI TCATGA 1 cut(s) 1480
PceI AGGCCT 1 cut(s) 2061
PciSI GCTCTTC 1 cut(s) 1059
PctI GAATGC 1 cut(s) 1456
PdmI GAANNNNTTC 2 cut(s) 112, 941
PfeI GAWTC 9 cut(s) 93, 268, 350, 449, 875, 1077, 1294, 2271, 2809
PflMI CCANNNNNTGG 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 1563
PkrI GCNGC 4 cut(s) 257, 1096, 2341, 2719
PpuMI RGGWCCY 2 cut(s) 517, 1265
Psp5II RGGWCCY 2 cut(s) 517, 1265
Psp6I CCWGG 3 cut(s) 135, 1563, 2417
PspGI CCWGG 3 cut(s) 135, 1563, 2417
PspN4I GGNNCC 8 cut(s) 778, 1266, 1805, 1818, 2001, 2040, 2259, 2652
PspPI GGNCC 7 cut(s) 517, 970, 1265, 1439, 1803, 1817, 2038
PspPPI RGGWCCY 2 cut(s) 517, 1265
PstI CTGCAG 1 cut(s) 1849
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 1 cut(s) 2650
RsaI GTAC 5 cut(s) 436, 1398, 2019, 2249, 2528
RsaNI GTAC 5 cut(s) 435, 1397, 2018, 2248, 2527
RseI CAYNNNNRTG 4 cut(s) 716, 1479, 1976, 2391
SapI GCTCTTC 1 cut(s) 1059
SatI GCNGC 4 cut(s) 256, 1095, 2340, 2718
Sau3AI GATC 7 cut(s) 703, 1164, 1787, 2431, 2650, 2758, 2762
Sau96I GGNCC 7 cut(s) 517, 970, 1265, 1439, 1803, 1817, 2038
ScaI AGTACT 1 cut(s) 436
ScrFI CCNGG 4 cut(s) 137, 213, 1565, 2419
SduI GDGCHC 2 cut(s) 1117, 2410
SfaNI GCATC 5 cut(s) 544, 922, 1882, 2269, 2299
SfcI CTRYAG 3 cut(s) 1185, 1494, 1845
SinI GGWCC 6 cut(s) 517, 970, 1265, 1439, 1803, 1817
SmiMI CAYNNNNRTG 4 cut(s) 716, 1479, 1976, 2391
SmlI CTYRAG 3 cut(s) 658, 998, 2489
SmoI CTYRAG 3 cut(s) 658, 998, 2489
SphI GCATGC 1 cut(s) 2829
SseBI AGGCCT 1 cut(s) 2061
SsiI CCGC 2 cut(s) 255, 1810
SspI AATATT 1 cut(s) 280
SspMI CTAG 6 cut(s) 252, 558, 1212, 1523, 2345, 2403
StuI AGGCCT 1 cut(s) 2061
StyD4I CCNGG 4 cut(s) 135, 211, 1563, 2417
StyI CCWWGG 4 cut(s) 103, 1602, 2062, 2721
TaaI ACNGT 6 cut(s) 955, 1478, 1729, 1753, 1799, 2548
TaiI ACGT 3 cut(s) 1197, 2073, 2118
TaqI TCGA 4 cut(s) 1080, 1608, 1890, 2761
TatI WGTACW 3 cut(s) 434, 2017, 2247
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 9 cut(s) 93, 268, 350, 449, 875, 1077, 1294, 2271, 2809
TscAI CASTG 4 cut(s) 322, 544, 1696, 2250
TseFI GTSAC 2 cut(s) 1370, 2066
TseI GCWGC 3 cut(s) 1094, 2339, 2717
Tsp45I GTSAC 2 cut(s) 1370, 2066
TspGWI ACGGA 2 cut(s) 1586, 1940
TspRI CASTG 4 cut(s) 322, 544, 1696, 2250
Van91I CCANNNNNTGG 1 cut(s) 476
VneI GTGCAC 1 cut(s) 2406
VpaK11BI GGWCC 6 cut(s) 517, 970, 1265, 1439, 1803, 1817
XagI CCTNNNNNAGG 1 cut(s) 2773
XapI RAATTY 4 cut(s) 45, 483, 940, 2348
XceI RCATGY 2 cut(s) 2443, 2829
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 1686
XmnI GAANNNNTTC 2 cut(s) 112, 941
XspI CTAG 6 cut(s) 252, 558, 1212, 1523, 2345, 2403
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 2 cut(s) 1897, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.