pycom111g01260

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0008681
Physical Location & Seq
Reverse (-)
807754 .. 810012
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGGAGCTCACTCCTCCGCCCCACCGTTGCATTTGGTCCTCGCCGATTCGTTCGTCCTCCTCCTCGTACCGACCTCTCCTCGTTTCAATTTCGCCTCGGAAGACCAAGCTCCTCAGGTCCGTACCTTCTTTTGGATCGATTCGCCGGAGAAATCTGTGCTCCGCCTCCAGCTCCGAGACGTTGGTTGGGTCGCGGAAGGAGGACGGCGGCAAGCAGAGTCAGGCGGTGAGCAAGAAAGACGAGGAGGAAGACTTGAAATCTTGGATGCACAAGAACGGGCTGCCTCCTTGCAAGGTCGTGCTCAAAGACAAACCTTCCTACGACGAGAAGCTCCGGCCCATTCATTACGTAGCTGCCAGCGAAGATCTTGAGGTTGGTGATGTCGCATTTTCGGTGCCGGATTCTTTAGTTGTAACTCTCAAGAGAGTGTTGGGGAACGAAACAATTGCTGAATTATTAACTACGAACAAATTATCGGAATTGGCATGCTTGGCATTGTATCTGATGTACGAGAAGAAACAAGGAAAGAAGTCATTTTGGTATCCCTATATAAGAGAGCTCGATCGTCAGCGAGGGAGGGGTCAATTGGCGGTGGAATCACCCCTTTTATGGTCAGAAGCTGAACTGGCTCATCTTACAGGCAGCCCCACAAAGGCTGACGTTCGTGCAAGGGCCGAAGGAATTAAAAGAGAATATAATGAGCTTGACACTGTCTGGTTTATGGCTGGTTCCCTATTTCAGCAATACCCATATGATATTCCTACGGAGGCCTTCCCATTTGAGATATTCAAACAAGCTTTTGTTGCAGTTCAGTCTTGTGTAGTGCATTTGCAGAAAGTTAGTTTGGCTCAAAGATTTGCTTTGGTTCCTCTTGGGCCCCCATTGTTAGCTTACGGAAGCAACTGTAGAGCAATGTTGACGGCTGTTGATGGTGCTGTTCAGCTAGTGGTCGATCGGCCATATAAGGCAGGGGAGTCTATTGTTGTGTGGTGTGGACCGCAGCCTAATTCAAAGTTGCTTATAAACTATGGTTTTGTTGACGAAGATAATTCTTATGACCGTTTGGTGGTTGAGGCAGCTTTGAACACTGAGGATCCACAATATCAAGATAAAAGAATGGTTGCTCAAAGAAATGGAAAATTATCAGTACAAACTTTTCAGGTATATGTGGGAAAGGAAAAGGAAACGGTCTCCGATATGCTTCCCTATCTGCGACTGGGCTATGTTTCAGATCCTTCAGAGATGCAATCTGTTATCTCCTCTCAAGGTCCAATTTGTCCAAAGAGACTGGAACGTAGAACATTTGGGGACCTTGTTAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

442

Amino Acids

49.51

Weight (kDa)

8.71

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 363 - 429 3.1e-10 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 1 cut(s) 193
AciI CCGC 7 cut(s) 17, 162, 193, 207, 224, 590, 998
AclWI GGATC 4 cut(s) 142, 1088, 1101, 1226
AcoI YGGCCR 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 1221
AfaI GTAC 4 cut(s) 68, 123, 509, 1149
AfiI CCNNNNNNNGG 5 cut(s) 131, 609, 652, 1066, 1318
AgsI TTSAA 5 cut(s) 87, 256, 790, 1011, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
Alw21I GWGCWC 4 cut(s) 9, 161, 303, 561
Alw26I GTCTC 3 cut(s) 170, 1195, 1279
AlwI GGATC 4 cut(s) 142, 1088, 1101, 1226
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 5 cut(s) 335, 672, 768, 875, 956
ApaI GGGCCC 1 cut(s) 879
ApeKI GCWGC 5 cut(s) 280, 353, 642, 1000, 1076
AspS9I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1309
AsuHPI GGTGA 3 cut(s) 238, 389, 591
AvaII GGWCC 5 cut(s) 36, 117, 995, 1268, 1309
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 879
BamHI GGATCC 1 cut(s) 1093
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 9, 561, 879
BbsI GAAGAC 2 cut(s) 107, 255
Bbv12I GWGCWC 4 cut(s) 9, 161, 303, 561
BbvI GCAGC 5 cut(s) 267, 340, 654, 1012, 1088
BccI CCATC 1 cut(s) 923
BceAI ACGGC 2 cut(s) 220, 936
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciVI GTATCC 1 cut(s) 552
BcoDI GTCTC 3 cut(s) 170, 1195, 1279
BfaI CTAG 1 cut(s) 944
BfmI CTRYAG 1 cut(s) 904
BfuI GTATCC 1 cut(s) 552
BglII AGATCT 1 cut(s) 364
BisI GCNGC 6 cut(s) 208, 281, 354, 643, 1001, 1077
BlsI GCNGC 6 cut(s) 209, 282, 355, 644, 1002, 1078
Bme18I GGWCC 5 cut(s) 36, 117, 995, 1268, 1309
BmgT120I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1309
BmiI GGNNCC 7 cut(s) 396, 730, 867, 877, 878, 1095, 1310
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 2 cut(s) 255, 1233
BmuI ACTGGG 1 cut(s) 1226
BpiI GAAGAC 2 cut(s) 107, 255
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 3 cut(s) 389, 404, 1248
Bsa29I ATCGAT 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 349
BsaI GGTCTC 1 cut(s) 1195
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 609, 652, 1066, 1318
Bse1I ACTGG 3 cut(s) 630, 1221, 1293
Bse21I CCTNAGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 918
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 131, 609, 652, 1066, 1318
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 2 cut(s) 127, 1080
BseNI ACTGG 3 cut(s) 630, 1221, 1293
BseRI GAGGAG 6 cut(s) 49, 52, 68, 101, 257, 1249
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 5 cut(s) 267, 340, 654, 1012, 1088
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 2 cut(s) 565, 955
BshFI GGCC 5 cut(s) 337, 674, 770, 877, 958
BshNI GGYRCC 1 cut(s) 394
BshVI ATCGAT 1 cut(s) 137
BsiEI CGRYCG 2 cut(s) 565, 955
BsiHKAI GWGCWC 4 cut(s) 9, 161, 303, 561
BsiSI CCGG 3 cut(s) 145, 334, 398
BslFI GGGAC 1 cut(s) 1322
BslI CCNNNNNNNGG 5 cut(s) 131, 609, 652, 1066, 1318
BsmAI GTCTC 3 cut(s) 170, 1195, 1279
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 1322
BsnI GGCC 5 cut(s) 337, 674, 770, 877, 958
Bso31I GGTCTC 1 cut(s) 1195
Bsp120I GGGCCC 1 cut(s) 875
Bsp1286I GDGCHC 5 cut(s) 9, 161, 303, 561, 879
Bsp143I GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
BspACI CCGC 7 cut(s) 17, 162, 193, 207, 224, 590, 998
BspANI GGCC 5 cut(s) 337, 674, 770, 877, 958
BspCNI CTCAG 2 cut(s) 126, 1081
BspDI ATCGAT 1 cut(s) 137
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 7 cut(s) 396, 730, 867, 877, 878, 1095, 1310
BspPI GGATC 4 cut(s) 142, 1088, 1101, 1226
BspT107I GGYRCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 1195
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 3 cut(s) 630, 1221, 1293
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
Bst4CI ACNGT 5 cut(s) 26, 712, 905, 1061, 1189
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 3 cut(s) 212, 358, 487
BstDEI CTNAG 2 cut(s) 113, 1089
BstENI CCTNNNNNAGG 1 cut(s) 1316
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 6 cut(s) 137, 367, 565, 955, 1096, 1234
BstMAI GTCTC 3 cut(s) 170, 1195, 1279
BstMBI GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
BstMCI CGRYCG 2 cut(s) 565, 955
BstMWI GCNNNNNNNGC 3 cut(s) 491, 626, 803
BstNSI RCATGY 1 cut(s) 489
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 349
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 5 cut(s) 267, 340, 654, 1012, 1088
BstV2I GAAGAC 2 cut(s) 107, 255
BstX2I RGATCY 3 cut(s) 364, 1093, 1231
BstYI RGATCY 3 cut(s) 364, 1093, 1231
Bsu15I ATCGAT 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 113
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 5 cut(s) 337, 674, 770, 877, 958
BsuTUI ATCGAT 1 cut(s) 137
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 708, 1086
Cac8I GCNNGC 3 cut(s) 212, 358, 487
CaiI CAGNNNCTG 1 cut(s) 620
Cfr13I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1309
ClaI ATCGAT 1 cut(s) 137
Csp6I GTAC 4 cut(s) 67, 122, 508, 1148
CviAII CATG 1 cut(s) 486
CviQI GTAC 4 cut(s) 67, 122, 508, 1148
DdeI CTNAG 2 cut(s) 113, 1089
DpnI GATC 6 cut(s) 136, 366, 564, 954, 1095, 1233
DpnII GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
EaeI YGGCCR 1 cut(s) 956
EciI GGCGGA 2 cut(s) 6, 151
Ecl136II GAGCTC 2 cut(s) 7, 559
Eco105I TACGTA 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 770
Eco24I GRGCYC 3 cut(s) 9, 561, 879
Eco31I GGTCTC 1 cut(s) 1195
Eco47I GGWCC 5 cut(s) 36, 117, 995, 1268, 1309
Eco53kI GAGCTC 2 cut(s) 7, 559
Eco57I CTGAAG 1 cut(s) 1221
Eco81I CCTNAGG 1 cut(s) 113
EcoICRI GAGCTC 2 cut(s) 7, 559
EcoNI CCTNNNNNAGG 1 cut(s) 1316
EcoO109I RGGNCCY 2 cut(s) 876, 1309
EcoT38I GRGCYC 3 cut(s) 9, 561, 879
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 1 cut(s) 489
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FaqI GGGAC 1 cut(s) 1322
FatI CATG 1 cut(s) 485
FauNDI CATATG 1 cut(s) 751
Fnu4HI GCNGC 6 cut(s) 208, 281, 354, 643, 1001, 1077
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 3 cut(s) 9, 561, 879
Fsp4HI GCNGC 6 cut(s) 208, 281, 354, 643, 1001, 1077
FspBI CTAG 1 cut(s) 944
GluI GCNGC 6 cut(s) 208, 281, 354, 643, 1001, 1077
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 5 cut(s) 337, 674, 770, 877, 958
HapII CCGG 3 cut(s) 145, 334, 398
Hin1II CATG 1 cut(s) 489
HincII GTYRAC 2 cut(s) 918, 1039
HindII GTYRAC 2 cut(s) 918, 1039
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 6 cut(s) 46, 139, 217, 401, 596, 974
HpaII CCGG 3 cut(s) 145, 334, 398
HphI GGTGA 3 cut(s) 238, 389, 591
Hpy166II GTNNAC 3 cut(s) 918, 995, 1039
Hpy188I TCNGA 8 cut(s) 99, 175, 478, 504, 616, 1195, 1231, 1240
Hpy188III TCNNGA 3 cut(s) 368, 421, 1106
Hpy8I GTNNAC 3 cut(s) 918, 995, 1039
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 6 cut(s) 135, 190, 324, 671, 781, 1245
HpyCH4III ACNGT 5 cut(s) 26, 712, 905, 1061, 1189
HpyCH4IV ACGT 4 cut(s) 179, 348, 660, 1294
HpyCH4V TGCA 8 cut(s) 30, 268, 291, 668, 806, 826, 832, 1246
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 626, 803
HpyF3I CTNAG 2 cut(s) 113, 1089
HpySE526I ACGT 4 cut(s) 179, 348, 660, 1294
Hsp92II CATG 1 cut(s) 489
Kzo9I GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
LmnI GCTCC 5 cut(s) 4, 114, 164, 176, 336
Lsp1109I GCAGC 5 cut(s) 267, 340, 654, 1012, 1088
LweI GCATC 2 cut(s) 255, 1233
MaeI CTAG 1 cut(s) 944
MaeII ACGT 4 cut(s) 179, 348, 660, 1294
MaeIII GTNAC 1 cut(s) 412
MalI GATC 6 cut(s) 136, 366, 564, 954, 1095, 1233
MboI GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
MboII GAAGA 5 cut(s) 112, 260, 374, 526, 1055
MfeI CAATTG 2 cut(s) 444, 584
MflI RGATCY 3 cut(s) 364, 1093, 1231
MhlI GDGCHC 5 cut(s) 9, 161, 303, 561, 879
MlyI GAGTC 2 cut(s) 226, 983
MseI TTAA 4 cut(s) 458, 684, 1317, 1324
MspI CCGG 3 cut(s) 145, 334, 398
MunI CAATTG 2 cut(s) 444, 584
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 491, 626, 803
NdeI CATATG 1 cut(s) 751
NdeII GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
NlaIII CATG 1 cut(s) 489
NlaIV GGNNCC 7 cut(s) 396, 730, 867, 877, 878, 1095, 1310
NspI RCATGY 1 cut(s) 489
PaeI GCATGC 1 cut(s) 489
PceI AGGCCT 1 cut(s) 770
PfeI GAWTC 4 cut(s) 46, 139, 401, 596
PflFI GACNNNGTC 1 cut(s) 710
PkrI GCNGC 6 cut(s) 209, 282, 355, 644, 1002, 1078
Ple19I CGATCG 2 cut(s) 565, 955
PleI GAGTC 2 cut(s) 225, 982
PpsI GAGTC 2 cut(s) 225, 982
Ppu21I YACGTR 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 1309
PsiI TTATAA 1 cut(s) 1022
Psp124BI GAGCTC 2 cut(s) 9, 561
Psp5II RGGWCCY 1 cut(s) 1309
PspN4I GGNNCC 7 cut(s) 396, 730, 867, 877, 878, 1095, 1310
PspOMI GGGCCC 1 cut(s) 875
PspPI GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1309
PspPPI RGGWCCY 1 cut(s) 1309
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 364, 1093, 1231
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 2 cut(s) 565, 955
RsaI GTAC 4 cut(s) 68, 123, 509, 1149
RsaNI GTAC 4 cut(s) 67, 122, 508, 1148
SacI GAGCTC 2 cut(s) 9, 561
SaqAI TTAA 4 cut(s) 458, 684, 1317, 1324
SatI GCNGC 6 cut(s) 208, 281, 354, 643, 1001, 1077
Sau3AI GATC 6 cut(s) 134, 364, 562, 952, 1093, 1231
Sau96I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1309
SchI GAGTC 2 cut(s) 226, 983
SduI GDGCHC 5 cut(s) 9, 161, 303, 561, 879
SfaNI GCATC 2 cut(s) 255, 1233
SfcI CTRYAG 1 cut(s) 904
SinI GGWCC 5 cut(s) 36, 117, 995, 1268, 1309
SmlI CTYRAG 3 cut(s) 368, 419, 1263
SmoI CTYRAG 3 cut(s) 368, 419, 1263
SnaBI TACGTA 1 cut(s) 349
SphI GCATGC 1 cut(s) 489
SseBI AGGCCT 1 cut(s) 770
SsiI CCGC 7 cut(s) 17, 162, 193, 207, 224, 590, 998
SspMI CTAG 1 cut(s) 944
SstI GAGCTC 2 cut(s) 9, 561
StuI AGGCCT 1 cut(s) 770
TaaI ACNGT 5 cut(s) 26, 712, 905, 1061, 1189
TaiI ACGT 4 cut(s) 182, 351, 663, 1297
TaqI TCGA 3 cut(s) 137, 561, 951
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 4 cut(s) 46, 139, 401, 596
Tru1I TTAA 4 cut(s) 458, 684, 1317, 1324
Tru9I TTAA 4 cut(s) 458, 684, 1317, 1324
TscAI CASTG 2 cut(s) 715, 1093
TseI GCWGC 5 cut(s) 280, 353, 642, 1000, 1076
TspDTI ATGAA 1 cut(s) 332
TspGWI ACGGA 3 cut(s) 109, 779, 909
TspRI CASTG 2 cut(s) 715, 1093
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 5 cut(s) 36, 117, 995, 1268, 1309
XagI CCTNNNNNAGG 1 cut(s) 1316
XceI RCATGY 1 cut(s) 489
XspI CTAG 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.