pycom111g05090

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0011545
Physical Location & Seq
Forward (+)
3281562 .. 3284036
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2379 bp
ATGATGAAAACTAAGAAGAGGAACCAAAAGGTATCAAAATGCTTGCTCTCTCACCAATCGCTTACAGTCATGAATCGTATTAAATCCGGGTTCGGATCATTGCCCTCCAAAACCCATATTAGATTTCTCTGCACATCGATTCTCGACGACCCACGCCCTCAATCTCCATTTCTGTCAGAAAAGCTCGCTTCTTTCTTGGACAGTTGTTCAGACGCTTTTTCACTGAGAAAACTCCACGCGCGCATTTTCGCTCACGGCCTCGGAAACAATATATTTCTCGGCTGCAAGCTCCTGAATTGCTACGCAAAGTTCGACCTTTTATCAGATTCCAGATGGGTTTTCGACAGAATCGTCAACGGGAATCTGTCTCTCTGGAATGCAATTCTTGTTGGGTATTTCAGAGCCGGTCAATTCGACGAAGTTCTTCGGCGGTACGTAGATTTGAGGCGGTGGAATATCGGGTTGGATAGCGGTGCCATTACGTTTGCTTTGAAAAGTTGTATCGAGTTGGGATATTTGGGATTTGGACTAGGGATTCATGGGGATGCTATGAAGTCTGGGTTTTGTTCAAATGGATTTGTGGGTTCGTCGCTCATCGGGTTGCATTCTAGATGTGGGTTTGTTGATGAAGCAGCTAAAGTGTTCGATGAAATTACTGAGAGAGATATTGTTGTCTACACTTCGATTATTACTGGTTATGCTCAGAGTGGCGATCAACACGCTTGCGAGGCTTTTGGGTTTGCACGCCGTATGCAGAGGCAAGGATTGCATCCAAATCGGGTCACTCTTGTTAGCTTACTTCAGGCAGCTAGAAGAGGAATTGGTGGTTCGGATGAAGTATTCGAAACAAGTCTCGTGGACATGTATAACAAATGTGGATCCCCTAGAAGGGCAGCCTGCATTTTTGGCGAAATGGATAGAAAGACTATTGGTTCTTGGAATGTGATGATTGCTGGTTATCTTAAAATGGAACAGCCCTTGGAGGCTTTCCACCTTTTCTGTCAAGTGATGGAAGAACATTTTGTCCCGGATTTGATAACTTTATCCAATGGGATTCTGTCTTGTGCTTGCTTGAATTATTTGTGTCCAGGAAAGAGTATCCACGGTTACATTATTCGAGCTGGTATTCAGCTTGATCTATTGGCAGCCACCGCTCTGGTCGATCTGTACTCCAAATCAAACAAGTTGATCCAGTCCAGGCAACTATTTGATAGAATGGAGAAAAAGGATGCTATATCTTATGATGTGATGATGACGGGCTATCTCCACAATAATTATGCCAGCGAAGCTGTGGACACCTTTGTCCAAATGGTTGGAGAAGGTATTAAACCAAACCTAGGTTCCTTGTTGAGTGTAATTTCTGCTACTTCTGAGCTGAAAGGCATAAGAAAAGGCAAGTGCATCCACGGTCTTGTATTAAGACATGGGTTTGACTTGAATACTGAAGTTATGAATCAAATTATCTACATGTACGCAAAATGTGGTTGTTTAGACCATGCGAGGCAAATCTTTAACAAGGTAAGATATCGGGATTTGGTCTCATGGACTTCAATGATGATGGGTTATGTTTACCACGGCCATGCCGATGAAGCCGTAACATTGTTTCGATCGATGCAAATTGAGCTGGCGCAACATGATTTAGTTACTCTGATAACTTTGCTTCAGGCAATTTGTCAGCTTGGAAGTCTGAGTTTTGCAAAAGAAGTCCACTGTCACTTGTATCGAGCTGATATAAACAACGATATATCTCTCACCAATTCTTTAATCACGACTTATTCCAAGTGTGGGAAATTAAATATGGCTGCTAATCTATTTGAGCATGCAGTTGAACGGTGTCCGACATCATGGAACACGATGATCCTTGCATATGGGATGCACGGGAAATGCAAGAAGGCCCTAACGCTCTTTGAACAGATGAAAAGTGAGAAGGTTGTCCCTGATGAAGTAACCTTTACATCAGTTCTCACCGCTTGCAGCCATTCCGGTATGGTAAACGAGGGCCTACGAGTTTTCAAGTCCATGGTGGAGGAACATTCCATAATCCCACGTGAAGAACACTATGGTTGTATGGTCGATTTACTAAGCCGAGCAGGATTGCTTGAAGAAGCATATAATCTGATCAAATCCATGCCATCAGATCTTACTGCTTCTCCACTGAAAACCCTGCTTGCCGCTTGTAAAGTACACGGAAATACGGAGATGGGTGAGGTTTTGGGAAGGCGGCTCTTGGACTTAGATCCCGAGAACTCAAGCGCGATCGCCCTGGTGTCAAATTTGTATGCAGAAGGTGGAAAGTGGAACGAAGTAGTCGGAATAAGAAACACCGCAAAACAGAGAGGTCTTAAAAGAACTCCAGGTTATAGTCTGATACAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

793

Amino Acids

88.26

Weight (kDa)

8.54

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 511 - 559 8.8e-06 PPR repeat family
PPR PF01535 615 - 643 2.2e-07 PPR repeat
PPR_2 PF13041 615 - 659 4.3e-12 PPR repeat family
PPR_long PF17177 630 - 712 1.9e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 729 - 791 1.6e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 350, 1301
AccB1I GGYRCC 1 cut(s) 473
AccBSI CCGCTC 1 cut(s) 1154
AccI GTMKAC 1 cut(s) 675
AccII CGCG 3 cut(s) 239, 241, 2255
AciI CCGC 8 cut(s) 430, 448, 471, 1152, 1968, 2172, 2221, 2325
AclWI GGATC 6 cut(s) 103, 873, 886, 1183, 1852, 2231
AcoI YGGCCR 1 cut(s) 1576
AcsI RAATTY 1 cut(s) 2272
AcuI CTGAAG 3 cut(s) 785, 1464, 1646
AcvI CACGTG 1 cut(s) 2048
AfaI GTAC 4 cut(s) 434, 1169, 1472, 2184
AfiI CCNNNNNNNGG 4 cut(s) 888, 889, 1337, 1785
AflIII ACRYGT 2 cut(s) 861, 1467
AgsI TTSAA 9 cut(s) 493, 570, 1075, 1438, 1551, 1829, 1910, 2014, 2102
AjnI CCWGG 4 cut(s) 1087, 1196, 2262, 2353
AjuI GAANNNNNNNTTGG 4 cut(s) 446, 478, 1324, 1356
AloI GAACNNNNNNTCC 2 cut(s) 1008, 1040
Alw26I GTCTC 3 cut(s) 372, 857, 1543
AlwI GGATC 6 cut(s) 103, 873, 886, 1183, 1852, 2231
Ama87I CYCGRG 1 cut(s) 2240
AoxI GGCC 4 cut(s) 256, 1576, 1893, 1999
ApeKI GCWGC 7 cut(s) 282, 632, 806, 893, 1145, 1802, 1974
ApoI RAATTY 1 cut(s) 2272
AsiSI GCGATCGC 1 cut(s) 2259
Asp700I GAANNNNTTC 1 cut(s) 423
AspA2I CCTAGG 1 cut(s) 1336
AspLEI GCGC 4 cut(s) 241, 243, 1630, 2255
AspS9I GGNCC 2 cut(s) 1894, 1999
AsuC2I CCSGG 2 cut(s) 88, 1028
AsuHPI GGTGA 4 cut(s) 44, 1744, 1957, 2216
AsuII TTCGAA 1 cut(s) 843
AvaI CYCGRG 1 cut(s) 2240
AvrII CCTAGG 1 cut(s) 1336
BamHI GGATCC 1 cut(s) 878
BanI GGYRCC 1 cut(s) 473
BauI CACGAG 1 cut(s) 854
BbrPI CACGTG 1 cut(s) 2048
BbvI GCAGC 7 cut(s) 269, 644, 818, 905, 1157, 1789, 1986
BccI CCATC 5 cut(s) 327, 1003, 1552, 2140, 2194
BceAI ACGGC 4 cut(s) 271, 732, 1577, 1591
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 4 cut(s) 1089, 1198, 2264, 2355
BciVI GTATCC 1 cut(s) 1109
BclI TGATCA 1 cut(s) 2118
BcnI CCSGG 2 cut(s) 88, 1028
BcoDI GTCTC 3 cut(s) 372, 857, 1543
BfaI CTAG 5 cut(s) 530, 609, 810, 885, 1337
BfuI GTATCC 1 cut(s) 1109
BglII AGATCT 1 cut(s) 2137
BisI GCNGC 9 cut(s) 283, 633, 807, 894, 1146, 1803, 1975, 2172, 2222
BlnI CCTAGG 1 cut(s) 1336
BlsI GCNGC 9 cut(s) 284, 634, 808, 895, 1147, 1804, 1976, 2173, 2223
Bme1390I CCNGG 6 cut(s) 88, 1028, 1089, 1198, 2264, 2355
BmeT110I CYCGRG 1 cut(s) 2240
BmgT120I GGNCC 2 cut(s) 1894, 1999
BmiI GGNNCC 4 cut(s) 23, 475, 880, 1342
BmrFI CCNGG 6 cut(s) 88, 1028, 1089, 1198, 2264, 2355
BmsI GCATC 6 cut(s) 535, 778, 1219, 1410, 1602, 1863
BpmI CTGGAG 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 843
BpuEI CTTGAG 1 cut(s) 2233
BpuMI CCSGG 2 cut(s) 88, 1028
Bsa29I ATCGAT 2 cut(s) 137, 1610
BsaAI YACGTR 2 cut(s) 436, 2048
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 8 cut(s) 259, 978, 1102, 1336, 1405, 1573, 2019, 2262
BsaWI WCCGGW 1 cut(s) 1982
BsaXI ACNNNNNCTCC 2 cut(s) 2134, 2164
Bsc4I CCNNNNNNNGG 4 cut(s) 888, 889, 1337, 1785
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 2 cut(s) 697, 1192
Bse3DI GCAATG 1 cut(s) 98
BseBI CCWGG 4 cut(s) 1089, 1198, 2264, 2355
BseCI ATCGAT 2 cut(s) 137, 1610
BseDI CCNNGG 8 cut(s) 259, 978, 1102, 1336, 1405, 1573, 2019, 2262
BseGI GGATG 6 cut(s) 550, 769, 838, 1234, 1401, 1878
BseLI CCNNNNNNNGG 4 cut(s) 888, 889, 1337, 1785
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 5 cut(s) 215, 648, 716, 1362, 1679
BseNI ACTGG 2 cut(s) 697, 1192
BsePI GCGCGC 1 cut(s) 239
BseXI GCAGC 7 cut(s) 269, 644, 818, 905, 1157, 1789, 1986
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 3 cut(s) 239, 241, 2255
Bsh1285I CGRYCG 2 cut(s) 1610, 2259
BshFI GGCC 4 cut(s) 258, 1578, 1895, 2001
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 2 cut(s) 137, 1610
BsiEI CGRYCG 2 cut(s) 1610, 2259
BsiHKCI CYCGRG 1 cut(s) 2240
BsiSI CCGG 4 cut(s) 87, 405, 1028, 1983
BslFI GGGAC 2 cut(s) 1010, 1919
BslI CCNNNNNNNGG 4 cut(s) 888, 889, 1337, 1785
BsmAI GTCTC 3 cut(s) 372, 857, 1543
BsmFI GGGAC 2 cut(s) 1010, 1919
BsmI GAATGC 2 cut(s) 382, 604
BsnI GGCC 4 cut(s) 258, 1578, 1895, 2001
Bso31I GGTCTC 1 cut(s) 1543
BsoBI CYCGRG 1 cut(s) 2240
Bsp119I TTCGAA 1 cut(s) 843
Bsp19I CCATGG 1 cut(s) 2019
BspACI CCGC 8 cut(s) 430, 448, 471, 1152, 1968, 2172, 2221, 2325
BspANI GGCC 4 cut(s) 258, 1578, 1895, 2001
BspCNI CTCAG 5 cut(s) 216, 649, 715, 1363, 1680
BspDI ATCGAT 2 cut(s) 137, 1610
BspFNI CGCG 3 cut(s) 239, 241, 2255
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 4 cut(s) 23, 475, 880, 1342
BspPI GGATC 6 cut(s) 103, 873, 886, 1183, 1852, 2231
BspT104I TTCGAA 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 1543
BsrBI CCGCTC 1 cut(s) 1154
BsrDI GCAATG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 404
BsrI ACTGG 2 cut(s) 697, 1192
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 8 cut(s) 259, 978, 1102, 1336, 1405, 1573, 2019, 2262
BssHII GCGCGC 1 cut(s) 239
BssSI CACGAG 1 cut(s) 854
BssT1I CCWWGG 3 cut(s) 978, 1336, 2019
Bst2BI CACGAG 1 cut(s) 854
Bst2UI CCWGG 4 cut(s) 1089, 1198, 2264, 2355
Bst4CI ACNGT 6 cut(s) 67, 203, 1106, 1409, 1712, 1833
Bst6I CTCTTC 2 cut(s) 11, 808
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 2 cut(s) 436, 2048
BstBI TTCGAA 1 cut(s) 843
BstDEI CTNAG 8 cut(s) 12, 224, 657, 702, 1371, 1688, 2081, 2233
BstDSI CCRYGG 4 cut(s) 1102, 1405, 1573, 2019
BstF5I GGATG 6 cut(s) 550, 769, 838, 1234, 1401, 1878
BstFNI CGCG 3 cut(s) 239, 241, 2255
BstHHI GCGC 4 cut(s) 241, 243, 1630, 2255
BstMAI GTCTC 3 cut(s) 372, 857, 1543
BstMCI CGRYCG 2 cut(s) 1610, 2259
BstMWI GCNNNNNNNGC 7 cut(s) 728, 766, 906, 1151, 1286, 1589, 1621
BstNI CCWGG 4 cut(s) 1089, 1198, 2264, 2355
BstNSI RCATGY 3 cut(s) 865, 1471, 1823
BstSCI CCNGG 6 cut(s) 86, 1026, 1087, 1196, 2262, 2353
BstSNI TACGTA 1 cut(s) 436
BstUI CGCG 3 cut(s) 239, 241, 2255
BstV1I GCAGC 7 cut(s) 269, 644, 818, 905, 1157, 1789, 1986
BstX2I RGATCY 3 cut(s) 878, 2137, 2236
BstXI CCANNNNNNTGG 2 cut(s) 1156, 1313
BstYI RGATCY 3 cut(s) 878, 2137, 2236
Bsu15I ATCGAT 2 cut(s) 137, 1610
BsuI GTATCC 1 cut(s) 1109
BsuRI GGCC 4 cut(s) 258, 1578, 1895, 2001
BsuTUI ATCGAT 2 cut(s) 137, 1610
BtgI CCRYGG 4 cut(s) 1102, 1405, 1573, 2019
BtsCI GGATG 6 cut(s) 550, 769, 838, 1234, 1401, 1878
BtsIMutI CAGTG 3 cut(s) 221, 1708, 2153
CciI TCATGA 1 cut(s) 69
CfoI GCGC 4 cut(s) 241, 243, 1630, 2255
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 2 cut(s) 1894, 1999
ClaI ATCGAT 2 cut(s) 137, 1610
CseI GACGC 1 cut(s) 221
Csp6I GTAC 4 cut(s) 433, 1168, 1471, 2183
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviQI GTAC 4 cut(s) 433, 1168, 1471, 2183
DdeI CTNAG 8 cut(s) 12, 224, 657, 702, 1371, 1688, 2081, 2233
DrdI GACNNNNNNGTC 2 cut(s) 350, 1301
DseDI GACNNNNNNGTC 2 cut(s) 350, 1301
EaeI YGGCCR 1 cut(s) 1576
Eam1104I CTCTTC 2 cut(s) 11, 808
EarI CTCTTC 2 cut(s) 11, 808
Eco105I TACGTA 1 cut(s) 436
Eco130I CCWWGG 3 cut(s) 978, 1336, 2019
Eco31I GGTCTC 1 cut(s) 1543
Eco32I GATATC 1 cut(s) 1526
Eco57I CTGAAG 3 cut(s) 785, 1464, 1646
Eco72I CACGTG 1 cut(s) 2048
Eco88I CYCGRG 1 cut(s) 2240
EcoO109I RGGNCCY 2 cut(s) 1894, 1999
EcoRII CCWGG 4 cut(s) 1087, 1196, 2262, 2353
EcoRV GATATC 1 cut(s) 1526
EcoT14I CCWWGG 3 cut(s) 978, 1336, 2019
ErhI CCWWGG 3 cut(s) 978, 1336, 2019
FaqI GGGAC 2 cut(s) 1010, 1919
FauNDI CATATG 1 cut(s) 1867
FbaI TGATCA 1 cut(s) 2118
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 9 cut(s) 283, 633, 807, 894, 1146, 1803, 1975, 2172, 2222
FokI GGATG 6 cut(s) 557, 756, 845, 1241, 1388, 1885
Fsp4HI GCNGC 9 cut(s) 283, 633, 807, 894, 1146, 1803, 1975, 2172, 2222
FspBI CTAG 5 cut(s) 530, 609, 810, 885, 1337
GlaI GCGC 4 cut(s) 240, 242, 1629, 2254
GluI GCNGC 9 cut(s) 283, 633, 807, 894, 1146, 1803, 1975, 2172, 2222
GsuI CTGGAG 1 cut(s) 2337
HaeIII GGCC 4 cut(s) 258, 1578, 1895, 2001
HapII CCGG 4 cut(s) 87, 405, 1028, 1983
HgaI GACGC 1 cut(s) 221
HhaI GCGC 4 cut(s) 241, 243, 1630, 2255
Hin6I GCGC 4 cut(s) 239, 241, 1628, 2253
HinP1I GCGC 4 cut(s) 239, 241, 1628, 2253
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 8 cut(s) 73, 139, 326, 348, 361, 535, 1054, 1453
HpaII CCGG 4 cut(s) 87, 405, 1028, 1983
HphI GGTGA 4 cut(s) 44, 1744, 1957, 2216
Hpy166II GTNNAC 8 cut(s) 355, 676, 859, 1294, 1570, 1708, 1993, 2185
Hpy188III TCNNGA 9 cut(s) 70, 143, 292, 330, 373, 609, 1529, 1768, 2240
Hpy8I GTNNAC 8 cut(s) 355, 676, 859, 1294, 1570, 1708, 1993, 2185
Hpy99I CGWCG 3 cut(s) 149, 419, 592
HpyAV CCTTC 6 cut(s) 882, 1313, 1885, 1921, 2211, 2279
HpyCH4III ACNGT 6 cut(s) 67, 203, 1106, 1409, 1712, 1833
HpyCH4IV ACGT 3 cut(s) 435, 482, 2047
HpyF10VI GCNNNNNNNGC 7 cut(s) 728, 766, 906, 1151, 1286, 1589, 1621
HpyF3I CTNAG 8 cut(s) 12, 224, 657, 702, 1371, 1688, 2081, 2233
HpySE526I ACGT 3 cut(s) 435, 482, 2047
HspAI GCGC 4 cut(s) 239, 241, 1628, 2253
Ksp22I TGATCA 1 cut(s) 2118
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 7 cut(s) 269, 644, 818, 905, 1157, 1789, 1986
LweI GCATC 6 cut(s) 535, 778, 1219, 1410, 1602, 1863
MaeI CTAG 5 cut(s) 530, 609, 810, 885, 1337
MaeII ACGT 3 cut(s) 435, 482, 2047
MaeIII GTNAC 6 cut(s) 781, 1106, 1594, 1642, 1712, 1945
MbiI CCGCTC 1 cut(s) 1154
MboII GAAGA 6 cut(s) 28, 416, 825, 1025, 2063, 2114
MflI RGATCY 3 cut(s) 878, 2137, 2236
MmeI TCCRAC 4 cut(s) 444, 1294, 1862, 2290
MroXI GAANNNNTTC 1 cut(s) 423
MseI TTAA 8 cut(s) 81, 963, 1326, 1418, 1512, 1763, 1793, 2343
MslI CAYNNNNRTG 3 cut(s) 543, 1578, 1584
MspI CCGG 4 cut(s) 87, 405, 1028, 1983
MspR9I CCNGG 6 cut(s) 88, 1028, 1089, 1198, 2264, 2355
Mva1269I GAATGC 2 cut(s) 382, 604
MvaI CCWGG 4 cut(s) 1089, 1198, 2264, 2355
MvnI CGCG 3 cut(s) 239, 241, 2255
MwoI GCNNNNNNNGC 7 cut(s) 728, 766, 906, 1151, 1286, 1589, 1621
NciI CCSGG 2 cut(s) 88, 1028
NcoI CCATGG 1 cut(s) 2019
NdeI CATATG 1 cut(s) 1867
NlaIV GGNNCC 4 cut(s) 23, 475, 880, 1342
NmeAIII GCCGAG 2 cut(s) 258, 2111
NmuCI GTSAC 2 cut(s) 781, 1712
NspI RCATGY 3 cut(s) 865, 1471, 1823
NspV TTCGAA 1 cut(s) 843
PaeI GCATGC 1 cut(s) 1823
PagI TCATGA 1 cut(s) 69
PauI GCGCGC 1 cut(s) 239
PciI ACATGT 2 cut(s) 861, 1467
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 348, 1581
PctI GAATGC 2 cut(s) 382, 604
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 8 cut(s) 73, 139, 326, 348, 361, 535, 1054, 1453
PfoI TCCNGGA 2 cut(s) 1026, 1087
PkrI GCNGC 9 cut(s) 284, 634, 808, 895, 1147, 1804, 1976, 2173, 2223
Ple19I CGATCG 2 cut(s) 1610, 2259
PmaCI CACGTG 1 cut(s) 2048
PmlI CACGTG 1 cut(s) 2048
Ppu21I YACGTR 2 cut(s) 436, 2048
PscI ACATGT 2 cut(s) 861, 1467
Psp6I CCWGG 4 cut(s) 1087, 1196, 2262, 2353
PspCI CACGTG 1 cut(s) 2048
PspGI CCWGG 4 cut(s) 1087, 1196, 2262, 2353
PspN4I GGNNCC 4 cut(s) 23, 475, 880, 1342
PspPI GGNCC 2 cut(s) 1894, 1999
PsuI RGATCY 3 cut(s) 878, 2137, 2236
PteI GCGCGC 1 cut(s) 239
PvuI CGATCG 2 cut(s) 1610, 2259
RgaI GCGATCGC 1 cut(s) 2259
RsaI GTAC 4 cut(s) 434, 1169, 1472, 2184
RsaNI GTAC 4 cut(s) 433, 1168, 1471, 2183
RseI CAYNNNNRTG 3 cut(s) 543, 1578, 1584
SaqAI TTAA 8 cut(s) 81, 963, 1326, 1418, 1512, 1763, 1793, 2343
SatI GCNGC 9 cut(s) 283, 633, 807, 894, 1146, 1803, 1975, 2172, 2222
Sau96I GGNCC 2 cut(s) 1894, 1999
ScrFI CCNGG 6 cut(s) 88, 1028, 1089, 1198, 2264, 2355
SfaAI GCGATCGC 1 cut(s) 2259
SfaNI GCATC 6 cut(s) 535, 778, 1219, 1410, 1602, 1863
SfuI TTCGAA 1 cut(s) 843
SgfI GCGATCGC 1 cut(s) 2259
SmiMI CAYNNNNRTG 3 cut(s) 543, 1578, 1584
SmlI CTYRAG 1 cut(s) 2248
SmoI CTYRAG 1 cut(s) 2248
SnaBI TACGTA 1 cut(s) 436
SphI GCATGC 1 cut(s) 1823
SsiI CCGC 8 cut(s) 430, 448, 471, 1152, 1968, 2172, 2221, 2325
SspMI CTAG 5 cut(s) 530, 609, 810, 885, 1337
StyD4I CCNGG 6 cut(s) 86, 1026, 1087, 1196, 2262, 2353
StyI CCWWGG 3 cut(s) 978, 1336, 2019
TaaI ACNGT 6 cut(s) 67, 203, 1106, 1409, 1712, 1833
TaiI ACGT 3 cut(s) 438, 485, 2050
TatI WGTACW 2 cut(s) 1167, 2182
TauI GCSGC 2 cut(s) 2174, 2224
TfiI GAWTC 8 cut(s) 73, 139, 326, 348, 361, 535, 1054, 1453
Tru1I TTAA 8 cut(s) 81, 963, 1326, 1418, 1512, 1763, 1793, 2343
Tru9I TTAA 8 cut(s) 81, 963, 1326, 1418, 1512, 1763, 1793, 2343
TscAI CASTG 3 cut(s) 228, 1715, 2160
TseFI GTSAC 2 cut(s) 781, 1712
TseI GCWGC 7 cut(s) 282, 632, 806, 893, 1145, 1802, 1974
Tsp45I GTSAC 2 cut(s) 781, 1712
TspGWI ACGGA 2 cut(s) 2202, 2210
TspRI CASTG 3 cut(s) 228, 1715, 2160
XapI RAATTY 1 cut(s) 2272
XbaI TCTAGA 1 cut(s) 608
XceI RCATGY 3 cut(s) 865, 1471, 1823
XcmI CCANNNNNNNNNTGG 1 cut(s) 1288
XmaJI CCTAGG 1 cut(s) 1336
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 1 cut(s) 423
XspI CTAG 5 cut(s) 530, 609, 810, 885, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.