Rorug01G0067500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011545
Physical Location & Seq
Forward (+)
10987666 .. 10988253
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0067500.1

Sequence Viewer

Length: 588 bp
ATGCTTTCATTTTGGAAGAGGTTGTGGAAAGCCACTGTACCTGGTATAATGAAGATTTTTATGTGGAAATTGGTACAACAATGCCTTCCTACAATTGACTTACTAATAGCGAGGCATGTGCCCATTGAGAATACATTATGTGTGTTGTGCTTACAAGATTCAGAGTTTGCTGATCACTTTTGTCGTCAGTGCACATTTTCTAAACAAGTGTTGCAGTCAAACTCTAGTCTACAACAGGTGTGCTTTTCGCATGAAGCATTGGATTTAGATTTAAAGGAATGGTTGCAGCATTGTTCTAAGATGTTGACTGATGATAATTGGAATATATTTATATACTTTCTGTGGGGACTTTGGAAAGAGAGAAATACAAGAATTTGGGATGACAAGGCAATGCGTGCAGGTGATGTTGTTTTTGCTCTTAATGCAAGACTCTTTGATTATACTTTCCACAATAGACCTGTTTTGCATCCTATTTTGAAGGAGGCAAGGTGGAAACCTCCTCCTGAGGGGTGGGTTAAGATAAATCTGGATGGTACGTTTGACGTTGCTACAGTTCCAGCTGGGGGAGGCTCTTCACCTACATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

195

Amino Acids

22.78

Weight (kDa)

7.62

Isoelectric Point (pI)

56.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 3 - 70 2.6e-12 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 229
AcsI RAATTY 1 cut(s) 372
AfaI GTAC 3 cut(s) 39, 75, 535
AfiI CCNNNNNNNGG 2 cut(s) 506, 563
AgsI TTSAA 1 cut(s) 478
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 1 cut(s) 560
AluI AGCT 1 cut(s) 560
Alw21I GWGCWC 1 cut(s) 194
Alw44I GTGCAC 1 cut(s) 190
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 413, 567
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 2 cut(s) 123, 194
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 298
BccI CCATC 1 cut(s) 524
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 42
BclI TGATCA 1 cut(s) 172
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 389
BisI GCNGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 506, 563
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 1 cut(s) 42
BseGI GGATG 3 cut(s) 385, 466, 535
BseLI CCNNNNNNNGG 2 cut(s) 506, 563
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 495
BseRI GAGGAG 1 cut(s) 489
BseSI GKGCMC 2 cut(s) 123, 194
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 194
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 2 cut(s) 506, 563
BsmFI GGGAC 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 123, 194
Bsp143I GATC 1 cut(s) 172
BspCNI CTCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 389
BspQI GCTCTTC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 396
BssMI GATC 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 37, 553
Bst6I CTCTTC 2 cut(s) 11, 577
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 2 cut(s) 297, 504
BstF5I GGATG 3 cut(s) 385, 466, 535
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 395, 422
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 2 cut(s) 123, 194
BstV1I GCAGC 1 cut(s) 298
Bsu36I CCTNAGG 1 cut(s) 504
BtsCI GGATG 3 cut(s) 385, 466, 535
BtsIMutI CAGTG 2 cut(s) 33, 194
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 396
CsiI ACCWGGT 1 cut(s) 40
Csp6I GTAC 3 cut(s) 38, 74, 534
CviAII CATG 3 cut(s) 116, 251, 582
CviJI RGCY 3 cut(s) 32, 560, 570
CviKI_1 RGCY 3 cut(s) 32, 560, 570
CviQI GTAC 3 cut(s) 38, 74, 534
DdeI CTNAG 2 cut(s) 297, 504
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
DraI TTTAAA 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 11, 577
EarI CTCTTC 2 cut(s) 11, 577
Eco81I CCTNAGG 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 40
FaeI CATG 3 cut(s) 119, 254, 585
FaqI GGGAC 1 cut(s) 360
FatI CATG 3 cut(s) 115, 250, 581
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 3 cut(s) 392, 453, 542
Fsp4HI GCNGC 1 cut(s) 287
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 287
GsaI CCCAGC 1 cut(s) 564
Hin1II CATG 3 cut(s) 119, 254, 585
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 2 cut(s) 158, 429
HphI GGTGA 2 cut(s) 413, 567
Hpy166II GTNNAC 3 cut(s) 192, 230, 306
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 2 cut(s) 503, 527
Hpy8I GTNNAC 3 cut(s) 192, 230, 306
HpyAV CCTTC 2 cut(s) 95, 472
HpyCH4III ACNGT 2 cut(s) 37, 553
HpyCH4IV ACGT 2 cut(s) 536, 543
HpyCH4V TGCA 6 cut(s) 192, 214, 286, 398, 425, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 422
HpyF3I CTNAG 2 cut(s) 297, 504
HpySE526I ACGT 2 cut(s) 536, 543
Hsp92II CATG 3 cut(s) 119, 254, 585
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 1 cut(s) 172
LguI GCTCTTC 1 cut(s) 577
LpnPI CCDG 9 cut(s) 27, 54, 221, 384, 471, 512, 516, 546, 570
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 1 cut(s) 475
MabI ACCWGGT 1 cut(s) 40
MaeI CTAG 1 cut(s) 225
MaeII ACGT 2 cut(s) 536, 543
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 3 cut(s) 28, 64, 564
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 123, 194
MluCI AATT 4 cut(s) 68, 93, 316, 372
MlyI GAGTC 1 cut(s) 423
MnlI CCTC 7 cut(s) 12, 105, 475, 499, 507, 510, 560
MseI TTAA 3 cut(s) 272, 420, 516
MslI CAYNNNNRTG 1 cut(s) 580
MspA1I CMGCKG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 395, 422
NdeII GATC 1 cut(s) 172
NlaIII CATG 3 cut(s) 119, 254, 585
NspI RCATGY 1 cut(s) 119
PaqCI CACCTGC 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 1 cut(s) 288
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 3 cut(s) 39, 75, 535
RsaNI GTAC 3 cut(s) 38, 74, 534
RseI CAYNNNNRTG 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 577
SaqAI TTAA 3 cut(s) 272, 420, 516
SatI GCNGC 1 cut(s) 287
Sau3AI GATC 1 cut(s) 172
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 2 cut(s) 123, 194
SexAI ACCWGGT 1 cut(s) 40
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 549
SmiMI CAYNNNNRTG 1 cut(s) 580
Sse9I AATT 4 cut(s) 68, 93, 316, 372
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 37, 553
TaiI ACGT 2 cut(s) 539, 546
TasI AATT 4 cut(s) 68, 93, 316, 372
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 3 cut(s) 272, 420, 516
Tru9I TTAA 3 cut(s) 272, 420, 516
TscAI CASTG 2 cut(s) 40, 194
TseI GCWGC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 65, 267
TspRI CASTG 2 cut(s) 40, 194
VneI GTGCAC 1 cut(s) 190
XapI RAATTY 1 cut(s) 372
XceI RCATGY 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 229
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.