pycom11g03040

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008375
Physical Location & Seq
Forward (+)
2387880 .. 2389393
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
ATGGATAGCCGTTACATTTTTCAACCAGAACTCATGTGCCAATCTGCTTCCGCTGCGGTTTCATTTTCCTCAAATTCTCCGGGAGATGGTGGTGCGGCAGTTAGTTTGGTCAGGACAAGGTCCTCTAGAGCTCCTCCTTCATTCTTAATGAGAATGGCTATGAGAATATCTAGAGCAAGGTGGTTCACCTTCTTGAGAAGAGTCTACCACTACCAAAATGGTTCGAGGTCCAATCTTGATCGATCAAATCCTTTCGATAACAGCACTTGGATGATGCTGGAATTCATGGCTTTGGTTGTTCAAATAGGCGTGACCACGATTACCTTGGCTGTTTCAAAGGAGGAGAGGCCGGTTTGGCCTATGAGAATTTGGATTGTTGGGTATGATATTGGTTGTGTTCTCAATATGTTGGTGCTCTTTGGGCGTTACAGGCTACTTTATCTGACTCAAAGTGATGGTTTCAACCTCTCCGATTTGGAACAGCAGAGAAGCACTGAAGATTCCAGGACCACACATTTGATGAACAGGTGTAGAACCTCACTCGAGCTCTTCTTTGCCGTATGGTTTGTGATGGGTAATGTTTGGGTCTTCGATTCTCGCTTTAGTTCGTTTACTGGAGCCCCGAAACTCCATGTGCTCTGCATCTCTCTGCTTGCTTGGAATGCCATTAGCTACTCCTTTCCCTTCCTGCTGTTTGTGCTGCTATGTTGCTGTGTACCCCTAATTAGCAGCCTCCTTGGATACAACATGAACATGGGATCCATCGATAAAGGGGCTTCTGATGACCAAATTTCTCAGCTTCCCAGCTGGAGATACAAAGAAGTTAACGCCAAAATAGAGCTCGGAAATGATTGCGATTCAGGATCCCTCAATGATCGATCCAGATTGCTGCATTTGCCTGGCAAAATACCGAGACAAGGAAGAAGTACGGCAGTTGCCATGTTCCCATATGTTTCACCTCAATTGCGTCGATCATTGGCTCAGAATCATATCTTGCTGTCCTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_11 zf-RING_2

Protein Analysis

337

Amino Acids

38.13

Weight (kDa)

9.4

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AciI CCGC 3 cut(s) 51, 56, 95
AclWI GGATC 5 cut(s) 753, 766, 858, 871, 873
AcsI RAATTY 4 cut(s) 73, 281, 366, 789
AcuI CTGAAG 1 cut(s) 516
AfaI GTAC 2 cut(s) 717, 928
AfiI CCNNNNNNNGG 2 cut(s) 86, 917
AgsI TTSAA 4 cut(s) 23, 302, 336, 463
AjnI CCWGG 2 cut(s) 503, 898
AloI GAACNNNNNNTCC 2 cut(s) 743, 775
AluBI AGCT 6 cut(s) 131, 547, 672, 799, 807, 841
AluI AGCT 6 cut(s) 131, 547, 672, 799, 807, 841
Alw21I GWGCWC 5 cut(s) 133, 417, 549, 639, 843
Alw26I GTCTC 1 cut(s) 907
AlwI GGATC 5 cut(s) 753, 766, 858, 871, 873
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 2 cut(s) 347, 356
ApeKI GCWGC 4 cut(s) 53, 700, 729, 889
ApoI RAATTY 4 cut(s) 73, 281, 366, 789
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AspS9I GGNCC 3 cut(s) 120, 228, 507
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 178, 948
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 3 cut(s) 120, 228, 507
BamHI GGATCC 2 cut(s) 758, 863
BanII GRGCYC 4 cut(s) 133, 549, 622, 843
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 5 cut(s) 133, 417, 549, 639, 843
BbvI GCAGC 4 cut(s) 40, 687, 741, 876
BccI CCATC 4 cut(s) 80, 449, 565, 770
BceAI ACGGC 2 cut(s) 542, 945
BciT130I CCWGG 2 cut(s) 505, 900
BciVI GTATCC 1 cut(s) 734
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 907
BfaI CTAG 2 cut(s) 126, 171
BfuI GTATCC 1 cut(s) 734
BglI GCCNNNNNGGC 1 cut(s) 355
BisI GCNGC 5 cut(s) 54, 96, 701, 730, 890
BlsI GCNGC 5 cut(s) 55, 97, 702, 731, 891
Bme1390I CCNGG 3 cut(s) 81, 505, 900
Bme18I GGWCC 3 cut(s) 120, 228, 507
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 3 cut(s) 120, 228, 507
BmiI GGNNCC 3 cut(s) 619, 760, 865
BmrFI CCNGG 3 cut(s) 81, 505, 900
BmsI GCATC 2 cut(s) 264, 651
BpiI GAAGAC 1 cut(s) 580
BpmI CTGGAG 2 cut(s) 636, 829
BpuEI CTTGAG 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 81
Bsa29I ATCGAT 3 cut(s) 241, 765, 877
BsaJI CCNNGG 2 cut(s) 324, 736
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 917
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 619
BseBI CCWGG 2 cut(s) 505, 900
BseCI ATCGAT 3 cut(s) 241, 765, 877
BseDI CCNNGG 2 cut(s) 324, 736
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 2 cut(s) 86, 917
BseMII CTCAG 2 cut(s) 809, 995
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 2 cut(s) 123, 356
BseXI GCAGC 4 cut(s) 40, 687, 741, 876
BseYI CCCAGC 1 cut(s) 803
BshFI GGCC 2 cut(s) 349, 358
BshVI ATCGAT 3 cut(s) 241, 765, 877
BsiHKAI GWGCWC 5 cut(s) 133, 417, 549, 639, 843
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 2 cut(s) 80, 350
BslI CCNNNNNNNGG 2 cut(s) 86, 917
BsmAI GTCTC 1 cut(s) 907
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 2 cut(s) 349, 358
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 6 cut(s) 133, 417, 549, 622, 639, 843
Bsp143I GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
BspACI CCGC 3 cut(s) 51, 56, 95
BspANI GGCC 2 cut(s) 349, 358
BspCNI CTCAG 2 cut(s) 808, 994
BspDI ATCGAT 3 cut(s) 241, 765, 877
BspLI GGNNCC 3 cut(s) 619, 760, 865
BspPI GGATC 5 cut(s) 753, 766, 858, 871, 873
BspQI GCTCTTC 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 1 cut(s) 619
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 324, 736
BssMI GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
BssT1I CCWWGG 2 cut(s) 324, 736
Bst2UI CCWGG 2 cut(s) 505, 900
Bst6I CTCTTC 2 cut(s) 193, 554
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 795, 981
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 7 cut(s) 241, 245, 761, 866, 877, 881, 974
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
BstMWI GCNNNNNNNGC 7 cut(s) 53, 355, 421, 430, 662, 697, 895
BstNI CCWGG 2 cut(s) 505, 900
BstSCI CCNGG 3 cut(s) 79, 503, 898
BstV1I GCAGC 4 cut(s) 40, 687, 741, 876
BstV2I GAAGAC 1 cut(s) 580
BstX2I RGATCY 2 cut(s) 758, 863
BstYI RGATCY 2 cut(s) 758, 863
Bsu15I ATCGAT 3 cut(s) 241, 765, 877
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 2 cut(s) 349, 358
BsuTUI ATCGAT 3 cut(s) 241, 765, 877
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 654
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 120, 228, 507
ClaI ATCGAT 3 cut(s) 241, 765, 877
CseI GACGC 1 cut(s) 956
Csp6I GTAC 2 cut(s) 716, 927
CviAII CATG 6 cut(s) 34, 286, 632, 748, 754, 940
CviQI GTAC 2 cut(s) 716, 927
DdeI CTNAG 2 cut(s) 795, 981
DpnI GATC 7 cut(s) 240, 244, 760, 865, 876, 880, 973
DpnII GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
Eam1104I CTCTTC 2 cut(s) 193, 554
EarI CTCTTC 2 cut(s) 193, 554
Ecl136II GAGCTC 3 cut(s) 131, 547, 841
Eco130I CCWWGG 2 cut(s) 324, 736
Eco24I GRGCYC 4 cut(s) 133, 549, 622, 843
Eco47I GGWCC 3 cut(s) 120, 228, 507
Eco53kI GAGCTC 3 cut(s) 131, 547, 841
Eco57I CTGAAG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 542
EcoICRI GAGCTC 3 cut(s) 131, 547, 841
EcoO109I RGGNCCY 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 503, 898
EcoT14I CCWWGG 2 cut(s) 324, 736
EcoT38I GRGCYC 4 cut(s) 133, 549, 622, 843
ErhI CCWWGG 2 cut(s) 324, 736
FaeI CATG 6 cut(s) 37, 289, 635, 751, 757, 943
FatI CATG 6 cut(s) 33, 285, 631, 747, 753, 939
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 54, 96, 701, 730, 890
FokI GGATG 1 cut(s) 283
FriOI GRGCYC 4 cut(s) 133, 549, 622, 843
Fsp4HI GCNGC 5 cut(s) 54, 96, 701, 730, 890
FspBI CTAG 2 cut(s) 126, 171
GluI GCNGC 5 cut(s) 54, 96, 701, 730, 890
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 2 cut(s) 636, 829
HaeIII GGCC 2 cut(s) 349, 358
HapII CCGG 2 cut(s) 80, 350
HgaI GACGC 1 cut(s) 956
Hin1II CATG 6 cut(s) 37, 289, 635, 751, 757, 943
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 6 cut(s) 201, 445, 500, 593, 857, 985
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 2 cut(s) 80, 350
HphI GGTGA 2 cut(s) 178, 948
Hpy166II GTNNAC 5 cut(s) 186, 205, 612, 716, 826
Hpy188I TCNGA 5 cut(s) 444, 472, 781, 845, 984
Hpy188III TCNNGA 7 cut(s) 112, 126, 171, 193, 236, 861, 882
Hpy8I GTNNAC 5 cut(s) 186, 205, 612, 716, 826
Hpy99I CGWCG 1 cut(s) 972
HpyAV CCTTC 3 cut(s) 147, 199, 694
HpyCH4V TGCA 2 cut(s) 642, 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 355, 421, 430, 662, 697, 895
HpyF3I CTNAG 2 cut(s) 795, 981
Hsp92II CATG 6 cut(s) 37, 289, 635, 751, 757, 943
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 2 cut(s) 136, 617
Lsp1109I GCAGC 4 cut(s) 40, 687, 741, 876
LweI GCATC 2 cut(s) 264, 651
MaeI CTAG 2 cut(s) 126, 171
MaeIII GTNAC 3 cut(s) 11, 310, 425
MalI GATC 7 cut(s) 240, 244, 760, 865, 876, 880, 973
MboI GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
MboII GAAGA 5 cut(s) 210, 509, 541, 580, 933
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 2 cut(s) 758, 863
MhlI GDGCHC 6 cut(s) 133, 417, 549, 622, 639, 843
MluCI AATT 6 cut(s) 73, 281, 366, 723, 789, 962
MlyI GAGTC 2 cut(s) 210, 439
MseI TTAA 2 cut(s) 146, 825
MslI CAYNNNNRTG 2 cut(s) 269, 752
MspA1I CMGCKG 2 cut(s) 53, 807
MspI CCGG 2 cut(s) 80, 350
MspR9I CCNGG 3 cut(s) 81, 505, 900
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 2 cut(s) 505, 900
MwoI GCNNNNNNNGC 7 cut(s) 53, 355, 421, 430, 662, 697, 895
NciI CCSGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 949
NdeII GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
NlaIII CATG 6 cut(s) 37, 289, 635, 751, 757, 943
NlaIV GGNNCC 3 cut(s) 619, 760, 865
NmuCI GTSAC 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 542
PciSI GCTCTTC 1 cut(s) 554
PctI GAATGC 1 cut(s) 667
PfeI GAWTC 4 cut(s) 500, 593, 857, 985
PflFI GACNNNGTC 1 cut(s) 118
PfoI TCCNGGA 2 cut(s) 79, 503
PkrI GCNGC 5 cut(s) 55, 97, 702, 731, 891
PleI GAGTC 2 cut(s) 209, 439
PpsI GAGTC 2 cut(s) 209, 439
PpuMI RGGWCCY 1 cut(s) 120
Psp124BI GAGCTC 3 cut(s) 133, 549, 843
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 503, 898
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 2 cut(s) 503, 898
PspN4I GGNNCC 3 cut(s) 619, 760, 865
PspPI GGNCC 3 cut(s) 120, 228, 507
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 542
PsuI RGATCY 2 cut(s) 758, 863
PsyI GACNNNGTC 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 807
RsaI GTAC 2 cut(s) 717, 928
RsaNI GTAC 2 cut(s) 716, 927
RseI CAYNNNNRTG 2 cut(s) 269, 752
SacI GAGCTC 3 cut(s) 133, 549, 843
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 2 cut(s) 146, 825
SatI GCNGC 5 cut(s) 54, 96, 701, 730, 890
Sau3AI GATC 7 cut(s) 238, 242, 758, 863, 874, 878, 971
Sau96I GGNCC 3 cut(s) 120, 228, 507
SchI GAGTC 2 cut(s) 210, 439
ScrFI CCNGG 3 cut(s) 81, 505, 900
SduI GDGCHC 6 cut(s) 133, 417, 549, 622, 639, 843
SfaNI GCATC 2 cut(s) 264, 651
SfiI GGCCNNNNNGGCC 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 542
SinI GGWCC 3 cut(s) 120, 228, 507
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 269, 752
SmlI CTYRAG 2 cut(s) 193, 542
SmoI CTYRAG 2 cut(s) 193, 542
Sse9I AATT 6 cut(s) 73, 281, 366, 723, 789, 962
SsiI CCGC 3 cut(s) 51, 56, 95
SspMI CTAG 2 cut(s) 126, 171
SstI GAGCTC 3 cut(s) 133, 549, 843
StyD4I CCNGG 3 cut(s) 79, 503, 898
StyI CCWWGG 2 cut(s) 324, 736
TaqI TCGA 8 cut(s) 224, 241, 255, 543, 591, 765, 877, 970
TasI AATT 6 cut(s) 73, 281, 366, 723, 789, 962
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 4 cut(s) 500, 593, 857, 985
Tru1I TTAA 2 cut(s) 146, 825
Tru9I TTAA 2 cut(s) 146, 825
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 4 cut(s) 53, 700, 729, 889
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 5 cut(s) 51, 129, 274, 536, 764
TspRI CASTG 1 cut(s) 499
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 3 cut(s) 120, 228, 507
XapI RAATTY 4 cut(s) 73, 281, 366, 789
XbaI TCTAGA 2 cut(s) 125, 170
XcmI CCANNNNNNNNNTGG 2 cut(s) 215, 322
XhoI CTCGAG 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 2 cut(s) 126, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.