pycom11g05550

Rab3 GTPase-activating protein catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004790
Physical Location & Seq
Reverse (-)
4225774 .. 4234091
8318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2925 bp
ATGGCGTCATCCAGCAGGTTGGAATCTGTCGGAGACGATGAAGCTGAAGAGGAGGAGGTTGAGCATTTTGATGATTTCACTCTTGCCTCTTCATGGGAAAGGTTTATTTCCGATATTGAAGCGGTTTGTAGGCAATGGTTGGCAGACGGTCCGAAAAATCTGCTGAAAAAGGATGCAAGGGAGGTTTCGGAGGATTTATACAAGGTCAAGTCTGAGTTGAAGTTTTCAGCAAAAACTTATTGCATGGAATACTATTTTGGAACAAAATATGACGGCAAAGTTATTGATTGGAATTCCACATTGCATGACTTGCAGTTGTGTTTTGGGGTGAAAGAATTTTTGGTAATTGCTCCTCAAAGTGCCAGCGGTGTGGTTCTTGATGCACCTGAGGCTAGCAAGCTTTTAAGTGCAGTTGCAATTGCCTTATCAAATTGTTCGAGTTTGTGGCCTGCATTTGTTCCAGTACATGTTCCTGCTAGGAAAGCTTACATCGGTATCCAAAATATGGGAACAGTTTTTACAAGAAGATTTGAGGCTGATCATATTGGTAGCCAGGTTCCAGTAAAACTCATGCATTTGGAAGGATTGTATGAGTTGTTTGTTTCCAAATTTGCCTACTCCACATTGGACTTTTCTACTCACAGTTTCAAAGTCCATTTCACAATGAAGCTAACCTACAGATCCCTTCCCCATGATGATGAGGTTCAAGGAGATGATCCAGATGTTACTGAATCTGAAATAGATCTTGGTGGTGAAACTCACAATACAACACAATGGGATGATGATTGTCCATGGAGTGAGTGGTATTCTGCCGAAGACCCTGTAAAAGGATTTGAATTGGTTGCCATGTGGTCAGAGAATTTGGTTGAGAGCTCTATGGAAATGGCTGAGCTGGAAAATTCTTCGCCCCACGAAGCTCAAAAATGGATACTATCTCCAAATATGTCTTCAACTTTAACTGATTCTTCCAAAGGAAACAGAATTGGATTTGCTTCTCAACTGTGCCTATTACTTGACACATTGAATATGTCCTTTGAGGCTCAATTTATGGAAGATTTTGTGTCAGTTGAAAATCCAGGTTCAGATAATTTGAAGTCCTCAATGGTTATACCTCCACCTACAGTTATTGATCGTATGCTTAAGGAACTCTTTCATGATGGTGCACGATTCCCAGATGTTGCTGCTGCGGAGCATAAGACTTCTCGAGCTATTAAGGGCGCACCTCTTAAATCCCTTTTTGCACAGTTTTGTTTGCATTCTCTATGGTTTGGAAACTGCAACATACGTGCTATTGCTGTTATTTGGATAGAGTTTGTTCGTGAAGTCCGGTGGTGTTGGGAAGAGTCACAGCCACTGCCCCTAATGCCAGCCACTGGTTCAATTGACCTCTCCACGTGTTTGATTAATCAGAAACTTCACATGCTTGCAACATGCATTGAGATGAAGCGTCAATTGAATGAAGACTTTCAAGATTGTGTTGGAAGCCAGGAGAATTCATCTCCTCAGACTGAGGAAGATAGTCAAGATGAGTATTCCTTTATAATGCAAACACCTGGTGAAAACTTTGATGGGAAACATGACAGCCCTGCAACACCAGATAATTTACAACACCCTGAAAAAAGTTTATCGAGTGGCAGCACTAAGCCGGAAGCTGTGGTATCTGCAAATCTGAAATCTTCAGATTCTACCAGGAGGGGTTCGGCTGGTGTGGCAGGATCTATGAAGCTGGAAGATGTGGTATCTGCTAATCTGAAATCTTCAGATTCTATCAGGAGGGGTTCAGCTGGTGTGGCAGGATCTATTATGCTTCTGAAGTCGTATCAGAGCATGCATGCGCCATACACTCAGGAATCACCACTTATGACAGAAGACATGCACGAAGAACGGCTTCATGCTGTTGAAGCCTTTGGCAACTCATTTGACTTTTCTGCTCAGTTGGAGAGAGAGATCTTAACTTCAGACATGTCAGCATTTAAAGCTGCAAATCCAGATGCTGTTTTTGAAGATTTTATTCGCTGGCATTCTCCCGGAGATTGGGAGAGCGATGACAAAAAAGATACTGGATCATCTAACACTCCGGCAATAGAGGGTTCAAAGGATAACTGGCCTCCTCAAGGAAGACTTTCAAAGAGAATGTCTGAGCATGGAAATTTATGGCGAAAGATTTGGAATGATGCACCTGCTTTGCCAGCTTATGAGCAGAAGCCACTTCTGGACCCAAATAGAGAAGGAGAAAAGATCCTTCATTATCTGGAAACATTACGACCGCATCAGTTGCTCGAGCAAATGATTTGTACTGCCTTCAGAGCATCAGCAGATACTTTGAACCAGACTAGCTACGGAGGCTTGAAGCAGATGGAAACCAAAATGGATCAACTTTACATTACTTTGGCGTCTGCATTGAGGCCTCTGCAAGCAAATCGTCTATCTGCTGGCAGTGAGACTATTGAAGATATCAGACGGCTCTGTGGTGTTTTTGAACATGTCGAGAAGCTACTCACTATTGCAGCTTCCCTTCATCGCAAGTTCTTGCAAGCCCCACGCCTCTCTGAAGCCATTTTCTCCGACTGCTGCAGCTTTTATTTTCCTAGAATGGCAACGAGCTCATCAGGAGACAATGCTCCAAAGGAGTTTGACAAGAAGCAACAAGTAAGGATGCACGAAAGAACCATCGTGTCGAATATGTTTACGCCTCCAACTGCTAATCAGTCGTGGAGGAAAGTTTTAAGCCTGGGTAATCTTCTCAACGGCCACGAACCAATCTTGAGGGAGATAATATTTTCCAAGCGGGATAGAGTGAGCGGAAATCACTACGCTGCCCACACATCCAAAATTACTCCAGAAGAAGTAGAAACATATAGAATGTATTCAAGTGGAACCTCGAACGATCTTCGCGTAGCTCTTTCCGTCGTTTCGTGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rab3-GTPase_cat

Protein Analysis

975

Amino Acids

109.26

Weight (kDa)

5.07

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rab3-GTPase_cat PF13890 588 - 752 1.4e-57 Rab3 GTPase-activating protein catalytic subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
AarI CACCTGC 1 cut(s) 2188
Acc36I ACCTGC 2 cut(s) 6, 2188
AccB7I CCANNNNNTGG 2 cut(s) 505, 1373
AccBSI CCGCTC 1 cut(s) 2802
AccII CGCG 1 cut(s) 2895
AciI CCGC 6 cut(s) 122, 366, 1187, 2267, 2788, 2802
AclWI GGATC 7 cut(s) 675, 710, 1723, 1804, 2071, 2233, 2379
AcoI YGGCCR 1 cut(s) 2749
AcsI RAATTY 7 cut(s) 292, 335, 608, 859, 898, 1492, 2149
AcuI CTGAAG 7 cut(s) 66, 1662, 1743, 1832, 1941, 2287, 2571
AcvI CACGTG 1 cut(s) 1395
AcyI GRCGYC 2 cut(s) 5, 2393
AdeI CACNNNGTG 1 cut(s) 1556
AfaI GTAC 2 cut(s) 465, 2296
AfiI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
AflII CTTAAG 1 cut(s) 1139
AflIII ACRYGT 4 cut(s) 466, 1394, 1962, 2482
AjnI CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2730
AjuI GAANNNNNNNTTGG 8 cut(s) 240, 272, 323, 355, 729, 761, 931, 963
Alw21I GWGCWC 3 cut(s) 875, 1165, 2606
Alw26I GTCTC 3 cut(s) 27, 2435, 2607
Alw44I GTGCAC 1 cut(s) 1161
AlwI GGATC 7 cut(s) 675, 710, 1723, 1804, 2071, 2233, 2379
AlwNI CAGNNNCTG 3 cut(s) 1354, 1373, 1994
Ama87I CYCGRG 2 cut(s) 1203, 2279
AoxI GGCC 4 cut(s) 446, 2105, 2405, 2749
ApaLI GTGCAC 1 cut(s) 1161
ApeKI GCWGC 8 cut(s) 1181, 1184, 1635, 1979, 2507, 2571, 2574, 2816
ApoI RAATTY 7 cut(s) 292, 335, 608, 859, 898, 1492, 2149
ArsI GACNNNNNNTTYG 2 cut(s) 2120, 2152
AseI ATTAAT 1 cut(s) 1404
Asp700I GAANNNNTTC 5 cut(s) 1149, 1464, 1887, 2122, 2866
AspLEI GCGC 2 cut(s) 1220, 1837
AspS9I GGNCC 2 cut(s) 149, 2215
AsuC2I CCSGG 1 cut(s) 2028
AsuHPI GGTGA 4 cut(s) 340, 764, 1568, 1845
AsuNHI GCTAGC 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 1203, 2279
AvaII GGWCC 2 cut(s) 149, 2215
AxyI CCTNAGG 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 1165
BaeI ACNNNNGTAYC 2 cut(s) 478, 511
BanII GRGCYC 2 cut(s) 875, 2606
BarI GAAGNNNNNNTAC 2 cut(s) 659, 691
BbrPI CACGTG 1 cut(s) 1395
BbsI GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2125
Bbv12I GWGCWC 3 cut(s) 875, 1165, 2606
BbvI GCAGC 8 cut(s) 1168, 1171, 1647, 1966, 2519, 2558, 2586, 2803
BccI CCATC 4 cut(s) 1151, 1562, 2350, 2678
BceAI ACGGC 4 cut(s) 289, 1901, 2477, 2764
BcgI CGANNNNNNTGC 4 cut(s) 142, 176, 2402, 2436
BciT130I CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2732
BciVI GTATCC 2 cut(s) 506, 921
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 2028
BcoDI GTCTC 3 cut(s) 27, 2435, 2607
BfaI CTAG 5 cut(s) 393, 477, 2334, 2589, 2923
BfmI CTRYAG 3 cut(s) 676, 1119, 2572
BfrI CTTAAG 1 cut(s) 1139
BfuAI ACCTGC 2 cut(s) 6, 2188
BfuI GTATCC 2 cut(s) 506, 921
BglII AGATCT 2 cut(s) 742, 1947
BisI GCNGC 8 cut(s) 1182, 1185, 1636, 1980, 2508, 2572, 2575, 2817
BlpI GCTNAGC 1 cut(s) 888
BlsI GCNGC 8 cut(s) 1183, 1186, 1637, 1981, 2509, 2573, 2576, 2818
Bme1390I CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2732
Bme18I GGWCC 2 cut(s) 149, 2215
BmeT110I CYCGRG 2 cut(s) 1203, 2279
BmgT120I GGNCC 2 cut(s) 149, 2215
BmiI GGNNCC 3 cut(s) 558, 2217, 2878
BmrFI CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2732
BmsI GCATC 7 cut(s) 163, 370, 1981, 2164, 2278, 2318, 2646
BmtI GCTAGC 1 cut(s) 396
BpiI GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2125
BpmI CTGGAG 1 cut(s) 2823
Bpu1102I GCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 2097, 2785
BpuMI CCSGG 1 cut(s) 2028
BsaAI YACGTR 2 cut(s) 1286, 1395
BsaHI GRCGYC 2 cut(s) 5, 2393
BsaJI CCNNGG 2 cut(s) 791, 2731
BsaWI WCCGGW 1 cut(s) 1326
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
Bse1I ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 2 cut(s) 140, 299
BseBI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2732
BseDI CCNNGG 2 cut(s) 791, 2731
BseGI GGATG 5 cut(s) 8, 178, 784, 2661, 2825
BseLI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
BseMI GCAATG 2 cut(s) 140, 299
BseMII CTCAG 8 cut(s) 204, 378, 879, 1500, 1517, 1859, 1946, 2130
BseNI ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
BseRI GAGGAG 5 cut(s) 65, 68, 342, 1491, 2100
BseSI GKGCMC 1 cut(s) 1165
BseXI GCAGC 8 cut(s) 1168, 1171, 1647, 1966, 2519, 2558, 2586, 2803
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 2895
Bsh1285I CGRYCG 1 cut(s) 2267
BshFI GGCC 4 cut(s) 448, 2107, 2407, 2751
BsiEI CGRYCG 1 cut(s) 2267
BsiHKAI GWGCWC 3 cut(s) 875, 1165, 2606
BsiHKCI CYCGRG 2 cut(s) 1203, 2279
BsiSI CCGG 4 cut(s) 1327, 1646, 2028, 2078
BslI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
BsmAI GTCTC 3 cut(s) 27, 2435, 2607
BsmBI CGTCTC 1 cut(s) 27
BsmI GAATGC 2 cut(s) 1255, 2020
BsnI GGCC 4 cut(s) 448, 2107, 2407, 2751
BsoBI CYCGRG 2 cut(s) 1203, 2279
Bsp1286I GDGCHC 3 cut(s) 875, 1165, 2606
Bsp1720I GCTNAGC 1 cut(s) 888
Bsp19I CCATGG 1 cut(s) 791
BspACI CCGC 6 cut(s) 122, 366, 1187, 2267, 2788, 2802
BspANI GGCC 4 cut(s) 448, 2107, 2407, 2751
BspCNI CTCAG 8 cut(s) 205, 379, 880, 1501, 1516, 1858, 1945, 2131
BspFNI CGCG 1 cut(s) 2895
BspHI TCATGA 1 cut(s) 1153
BspLI GGNNCC 3 cut(s) 558, 2217, 2878
BspMAI CTGCAG 1 cut(s) 2576
BspMI ACCTGC 2 cut(s) 6, 2188
BspOI GCTAGC 1 cut(s) 396
BspPI GGATC 7 cut(s) 675, 710, 1723, 1804, 2071, 2233, 2379
BspTI CTTAAG 1 cut(s) 1139
BsrBI CCGCTC 1 cut(s) 2802
BsrDI GCAATG 2 cut(s) 140, 299
BsrI ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
BssECI CCNNGG 2 cut(s) 791, 2731
BssNI GRCGYC 2 cut(s) 5, 2393
BssT1I CCWWGG 1 cut(s) 791
Bst2UI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2732
Bst4CI ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
Bst6I CTCTTC 3 cut(s) 42, 94, 1335
BstACI GRCGYC 2 cut(s) 5, 2393
BstAFI CTTAAG 1 cut(s) 1139
BstAPI GCANNNNNTGC 2 cut(s) 310, 2275
BstBAI YACGTR 2 cut(s) 1286, 1395
BstDEI CTNAG 9 cut(s) 213, 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
BstDSI CCRYGG 1 cut(s) 791
BstENI CCTNNNNNAGG 2 cut(s) 825, 2112
BstF5I GGATG 5 cut(s) 8, 178, 784, 2661, 2825
BstFNI CGCG 1 cut(s) 2895
BstHHI GCGC 2 cut(s) 1220, 1837
BstMAI GTCTC 3 cut(s) 27, 2435, 2607
BstMCI CGRYCG 1 cut(s) 2267
BstNI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2732
BstNSI RCATGY 8 cut(s) 470, 1423, 1434, 1831, 1835, 1876, 1966, 2486
BstSCI CCNGG 7 cut(s) 552, 1075, 1485, 1552, 1688, 2026, 2730
BstSFI CTRYAG 3 cut(s) 676, 1119, 2572
BstSLI GKGCMC 1 cut(s) 1165
BstUI CGCG 1 cut(s) 2895
BstV1I GCAGC 8 cut(s) 1168, 1171, 1647, 1966, 2519, 2558, 2586, 2803
BstV2I GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2125
BstX2I RGATCY 6 cut(s) 680, 742, 1715, 1796, 1947, 2238
BstXI CCANNNNNNTGG 2 cut(s) 19, 370
BstYI RGATCY 6 cut(s) 680, 742, 1715, 1796, 1947, 2238
Bsu36I CCTNAGG 1 cut(s) 387
BsuI GTATCC 2 cut(s) 506, 921
BsuRI GGCC 4 cut(s) 448, 2107, 2407, 2751
BtgI CCRYGG 1 cut(s) 791
BtgZI GCGATG 2 cut(s) 2058, 2504
BtsCI GGATG 5 cut(s) 8, 178, 784, 2661, 2825
BtsI GCAGTG 2 cut(s) 1352, 2443
BtsIMutI CAGTG 3 cut(s) 1352, 1371, 2443
BveI ACCTGC 2 cut(s) 6, 2188
CaiI CAGNNNCTG 3 cut(s) 1354, 1373, 1994
CciI TCATGA 1 cut(s) 1153
CfoI GCGC 2 cut(s) 1220, 1837
Cfr13I GGNCC 2 cut(s) 149, 2215
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 2 cut(s) 1436, 2382
CsiI ACCWGGT 1 cut(s) 1552
Csp6I GTAC 2 cut(s) 464, 2295
CspI CGGWCCG 1 cut(s) 149
CviQI GTAC 2 cut(s) 464, 2295
DdeI CTNAG 9 cut(s) 213, 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
DraI TTTAAA 1 cut(s) 1975
DraIII CACNNNGTG 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 2749
Eam1104I CTCTTC 3 cut(s) 42, 94, 1335
EarI CTCTTC 3 cut(s) 42, 94, 1335
Ecl136II GAGCTC 2 cut(s) 873, 2604
Eco130I CCWWGG 1 cut(s) 791
Eco147I AGGCCT 1 cut(s) 2407
Eco24I GRGCYC 2 cut(s) 875, 2606
Eco32I GATATC 1 cut(s) 2455
Eco47I GGWCC 2 cut(s) 149, 2215
Eco53kI GAGCTC 2 cut(s) 873, 2604
Eco57I CTGAAG 7 cut(s) 66, 1662, 1743, 1832, 1941, 2287, 2571
Eco72I CACGTG 1 cut(s) 1395
Eco81I CCTNAGG 1 cut(s) 387
Eco88I CYCGRG 2 cut(s) 1203, 2279
EcoICRI GAGCTC 2 cut(s) 873, 2604
EcoNI CCTNNNNNAGG 2 cut(s) 825, 2112
EcoRI GAATTC 2 cut(s) 292, 1492
EcoRII CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2730
EcoRV GATATC 1 cut(s) 2455
EcoT14I CCWWGG 1 cut(s) 791
EcoT22I ATGCAT 3 cut(s) 576, 1436, 1833
EcoT38I GRGCYC 2 cut(s) 875, 2606
ErhI CCWWGG 1 cut(s) 791
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 6 cut(s) 1133, 1165, 1872, 1904, 2106, 2138
FauI CCCGC 1 cut(s) 2781
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 8 cut(s) 1182, 1185, 1636, 1980, 2508, 2572, 2575, 2817
FokI GGATG 4 cut(s) 185, 791, 2668, 2812
FriOI GRGCYC 2 cut(s) 875, 2606
Fsp4HI GCNGC 8 cut(s) 1182, 1185, 1636, 1980, 2508, 2572, 2575, 2817
FspBI CTAG 5 cut(s) 393, 477, 2334, 2589, 2923
GlaI GCGC 2 cut(s) 1219, 1836
GluI GCNGC 8 cut(s) 1182, 1185, 1636, 1980, 2508, 2572, 2575, 2817
GsuI CTGGAG 1 cut(s) 2823
HaeIII GGCC 4 cut(s) 448, 2107, 2407, 2751
HapII CCGG 4 cut(s) 1327, 1646, 2028, 2078
HgaI GACGC 2 cut(s) 1436, 2382
HhaI GCGC 2 cut(s) 1220, 1837
Hin1I GRCGYC 2 cut(s) 5, 2393
Hin6I GCGC 2 cut(s) 1218, 1835
HinP1I GCGC 2 cut(s) 1218, 1835
HindIII AAGCTT 2 cut(s) 398, 483
HinfI GANTC 8 cut(s) 23, 731, 962, 1167, 1343, 1682, 1763, 1850
HpaII CCGG 4 cut(s) 1327, 1646, 2028, 2078
HphI GGTGA 4 cut(s) 340, 764, 1568, 1845
Hpy166II GTNNAC 2 cut(s) 1163, 2688
Hpy8I GTNNAC 2 cut(s) 1163, 2688
Hpy99I CGWCG 1 cut(s) 2912
HpyAV CCTTC 6 cut(s) 575, 695, 2222, 2252, 2311, 2525
HpyCH4III ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
HpyCH4IV ACGT 2 cut(s) 1285, 1394
HpyF3I CTNAG 9 cut(s) 213, 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
HpySE526I ACGT 2 cut(s) 1285, 1394
Hsp92I GRCGYC 2 cut(s) 5, 2393
HspAI GCGC 2 cut(s) 1218, 1835
Ksp22I TGATCA 1 cut(s) 538
LmnI GCTCC 3 cut(s) 355, 1189, 2626
Lsp1109I GCAGC 8 cut(s) 1168, 1171, 1647, 1966, 2519, 2558, 2586, 2803
LweI GCATC 7 cut(s) 163, 370, 1981, 2164, 2278, 2318, 2646
MabI ACCWGGT 1 cut(s) 1552
MaeI CTAG 5 cut(s) 393, 477, 2334, 2589, 2923
MaeII ACGT 2 cut(s) 1285, 1394
MaeIII GTNAC 3 cut(s) 724, 1344, 2918
MbiI CCGCTC 1 cut(s) 2802
MfeI CAATTG 3 cut(s) 417, 1380, 1451
MflI RGATCY 6 cut(s) 680, 742, 1715, 1796, 1947, 2238
MhlI GDGCHC 3 cut(s) 875, 1165, 2606
MlyI GAGTC 1 cut(s) 1352
MmeI TCCRAC 5 cut(s) 10, 1459, 1917, 2589, 2720
Mph1103I ATGCAT 3 cut(s) 576, 1436, 1833
MroXI GAANNNNTTC 5 cut(s) 1149, 1464, 1887, 2122, 2866
MseI TTAA 9 cut(s) 404, 956, 1140, 1212, 1227, 1404, 1952, 1974, 2726
MslI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
MspA1I CMGCKG 2 cut(s) 366, 1784
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 4 cut(s) 1327, 1646, 2028, 2078
MspR9I CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2732
MunI CAATTG 3 cut(s) 417, 1380, 1451
Mva1269I GAATGC 2 cut(s) 1255, 2020
MvaI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2732
MvnI CGCG 1 cut(s) 2895
NciI CCSGG 1 cut(s) 2028
NcoI CCATGG 1 cut(s) 791
NheI GCTAGC 1 cut(s) 392
NlaIV GGNNCC 3 cut(s) 558, 2217, 2878
NmuCI GTSAC 2 cut(s) 1344, 2918
NsiI ATGCAT 3 cut(s) 576, 1436, 1833
NspI RCATGY 8 cut(s) 470, 1423, 1434, 1831, 1835, 1876, 1966, 2486
PaeI GCATGC 2 cut(s) 1831, 1835
PaeR7I CTCGAG 2 cut(s) 1203, 2279
PagI TCATGA 1 cut(s) 1153
PaqCI CACCTGC 1 cut(s) 2188
PceI AGGCCT 1 cut(s) 2407
PciI ACATGT 3 cut(s) 466, 1962, 2482
PcsI WCGNNNNNNNCGW 1 cut(s) 2892
PctI GAATGC 2 cut(s) 1255, 2020
PdmI GAANNNNTTC 5 cut(s) 1149, 1464, 1887, 2122, 2866
PfeI GAWTC 7 cut(s) 23, 731, 962, 1167, 1682, 1763, 1850
PflMI CCANNNNNTGG 2 cut(s) 505, 1373
PfoI TCCNGGA 1 cut(s) 2026
PkrI GCNGC 8 cut(s) 1183, 1186, 1637, 1981, 2509, 2573, 2576, 2818
PleI GAGTC 1 cut(s) 1351
PmaCI CACGTG 1 cut(s) 1395
PmlI CACGTG 1 cut(s) 1395
PpsI GAGTC 1 cut(s) 1351
Ppu21I YACGTR 2 cut(s) 1286, 1395
PscI ACATGT 3 cut(s) 466, 1962, 2482
PshBI ATTAAT 1 cut(s) 1404
PsiI TTATAA 1 cut(s) 1541
Psp124BI GAGCTC 2 cut(s) 875, 2606
Psp6I CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2730
PspCI CACGTG 1 cut(s) 1395
PspGI CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2730
PspN4I GGNNCC 3 cut(s) 558, 2217, 2878
PspPI GGNCC 2 cut(s) 149, 2215
PspXI VCTCGAGB 1 cut(s) 2279
PsrI GAACNNNNNNTAC 2 cut(s) 502, 534
PstI CTGCAG 1 cut(s) 2576
PstNI CAGNNNCTG 3 cut(s) 1354, 1373, 1994
PsuI RGATCY 6 cut(s) 680, 742, 1715, 1796, 1947, 2238
PvuII CAGCTG 1 cut(s) 1784
RsaI GTAC 2 cut(s) 465, 2296
RsaNI GTAC 2 cut(s) 464, 2295
RseI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 2 cut(s) 875, 2606
SaqAI TTAA 9 cut(s) 404, 956, 1140, 1212, 1227, 1404, 1952, 1974, 2726
SatI GCNGC 8 cut(s) 1182, 1185, 1636, 1980, 2508, 2572, 2575, 2817
Sau96I GGNCC 2 cut(s) 149, 2215
SchI GAGTC 1 cut(s) 1352
ScrFI CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2732
SduI GDGCHC 3 cut(s) 875, 1165, 2606
SexAI ACCWGGT 1 cut(s) 1552
SfaNI GCATC 7 cut(s) 163, 370, 1981, 2164, 2278, 2318, 2646
SfcI CTRYAG 3 cut(s) 676, 1119, 2572
Sfr274I CTCGAG 2 cut(s) 1203, 2279
SinI GGWCC 2 cut(s) 149, 2215
SlaI CTCGAG 2 cut(s) 1203, 2279
SmiMI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
SmlI CTYRAG 5 cut(s) 1139, 1203, 2112, 2279, 2764
SmoI CTYRAG 5 cut(s) 1139, 1203, 2112, 2279, 2764
SphI GCATGC 2 cut(s) 1831, 1835
SseBI AGGCCT 1 cut(s) 2407
SsiI CCGC 6 cut(s) 122, 366, 1187, 2267, 2788, 2802
SspI AATATT 1 cut(s) 2778
SspMI CTAG 5 cut(s) 393, 477, 2334, 2589, 2923
SstI GAGCTC 2 cut(s) 875, 2606
StuI AGGCCT 1 cut(s) 2407
StyD4I CCNGG 7 cut(s) 552, 1075, 1485, 1552, 1688, 2026, 2730
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
TaiI ACGT 2 cut(s) 1288, 1397
TaqI TCGA 7 cut(s) 437, 1204, 1628, 2280, 2487, 2678, 2882
TatI WGTACW 2 cut(s) 463, 2294
TfiI GAWTC 7 cut(s) 23, 731, 962, 1167, 1682, 1763, 1850
Tru1I TTAA 9 cut(s) 404, 956, 1140, 1212, 1227, 1404, 1952, 1974, 2726
Tru9I TTAA 9 cut(s) 404, 956, 1140, 1212, 1227, 1404, 1952, 1974, 2726
TscAI CASTG 3 cut(s) 1359, 1378, 2443
TseFI GTSAC 2 cut(s) 1344, 2918
TseI GCWGC 8 cut(s) 1181, 1184, 1635, 1979, 2507, 2571, 2574, 2816
Tsp45I GTSAC 2 cut(s) 1344, 2918
TspGWI ACGGA 2 cut(s) 2355, 2896
TspRI CASTG 3 cut(s) 1359, 1378, 2443
Van91I CCANNNNNTGG 2 cut(s) 505, 1373
Vha464I CTTAAG 1 cut(s) 1139
VneI GTGCAC 1 cut(s) 1161
VpaK11BI GGWCC 2 cut(s) 149, 2215
VspI ATTAAT 1 cut(s) 1404
XagI CCTNNNNNAGG 2 cut(s) 825, 2112
XapI RAATTY 7 cut(s) 292, 335, 608, 859, 898, 1492, 2149
XceI RCATGY 8 cut(s) 470, 1423, 1434, 1831, 1835, 1876, 1966, 2486
XcmI CCANNNNNNNNNTGG 1 cut(s) 798
XhoI CTCGAG 2 cut(s) 1203, 2279
XmnI GAANNNNTTC 5 cut(s) 1149, 1464, 1887, 2122, 2866
XspI CTAG 5 cut(s) 393, 477, 2334, 2589, 2923
Zsp2I ATGCAT 3 cut(s) 576, 1436, 1833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.