Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0014315

Physical Location & Seq

Genomic Coordinates Reverse (-)

5391102 .. 5394731

Gene Structure 3630 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGATCCTACACTTCAGTCTCAGATTGATCAGGCAATGATGGCCCTCGATAAAACAGAAAAGAAGAGCGAACTTGGAGTAAATGCCATATTGGCTGTGTCAATTGCTGCTTGCAAAGCTGGAGCTGCTCAAAAGGAGGTTCCACTGTACAAACATATTGCTGACCTTTCTGGAAAAGGCCACCTGACCCTGCCTGTCCCTGCTTTTACTGTTATTAGTGGAGGAAAGCGTGCTGGGAATAATCTGGCCATTCAGGAAATTATGATTCTCCCAATTGGAGCTAGCAAGTTTGAGGAGGCATTGCAAATGCGATCTGAAACCTATCATCACCTAAAGGCTGTTATTACAGAAAAATATGGTGCACATGGATGTAATGTTGGTGAAGATGGTGGTTTTGCTCCCAACATCTCGAGTGTTAAAGAAGTCTTGGATCTTGTAAAAGAGGCGATAAGTAGAACAGATTATAAAGACCAAATCAAACTAGCAATTGATGTTGCTGCTACTGACTTTTGCATAGGTACAAAGTACGATCTGGATTACAAATCAGCGGATAAGGGACAAAATTTCAAGTCGGCTGATGATATGATTGGGATGTATAAAGAACTTTGTAGTGAGTACCCAATAGTGTCAGTTGAAGATCCGTTTGATAAAGAGGACTGGGAACACACTAAACGCTTTTCTAGTCTTGGAATTTGTCAGGTAGTTGGAGATGACTTGATAACTTCAAATCCAAAGCGTATTAAGAGAGCTATAGAGGAGTCCACCTGCAATTCTCTACTCATAAAGATAAACCAGATTGGAACATTGACCGAGATCATTGAAGTGATGAAGCTGGCAAAGGATGCCCACTGGGGAGTGGTCATGTCTCATAGATGTGGTGAAACTGAAGATTCCTTCATAGCCGACTTAGCTGTTGGTCTCTCAACAGGTCAGATCAAAGCTGGTGCTCCTTGCAGAGGCGAACGGCTGGCCAAGTACAACCAGTTGCTTCGAATTGAGGAAGAACTTGGGGACGATGCATTTTATGCTGGTGAAGATTGGAGGCAATCACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

352

Amino Acids

38.49

Weight (kDa)

5.24

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	1 - 53	1.5e-17	Enolase, N-terminal domain
Enolase_C	PF00113	62 - 349	2.5e-119	Enolase, C-terminal TIM barrel domain
MR_MLE_C	PF13378	191 - 314	2.1e-08	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014315)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29560
fragaria_vesca	FvH4_3g37690
malus_domestica	MD03G1078500.v1.1
prunus_persica	Prupe.6G063500_v2.0.a1
pyrus_communis	pycom03g06290 pycom11g07120
rosa_chinensis	RchiOBHm_Chr5g0067961
rosa_laevigata	RLG00000035947
rosa_roxburghii	Rroxscaffold_1G00012640 Rroxscaffold_1G00013350
rosa_rugosa	Rorug05G0386800
rosa_samantha	Rh5AG445700 Rh5BG463200 Rh5CG485100 Rh5DG477200
rosa_wichuraiana	Rw0G015360 Rw5G041620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	465
AarI	CACCTGC	1 cut(s)	773
Acc36I	ACCTGC	1 cut(s)	773
AciI	CCGC	1 cut(s)	548
AclWI	GGATC	3 cut(s)	12, 438, 632
AcoI	YGGCCR	2 cut(s)	246, 969
AcsI	RAATTY	2 cut(s)	562, 690
AcuI	CTGAAG	1 cut(s)	906
AfaI	GTAC	5 cut(s)	149, 520, 527, 617, 977
AfiI	CCNNNNNNNGG	1 cut(s)	956
AgsI	TTSAA	4 cut(s)	568, 635, 726, 821
AluBI	AGCT	7 cut(s)	119, 125, 281, 749, 832, 911, 941
AluI	AGCT	7 cut(s)	119, 125, 281, 749, 832, 911, 941
Alw21I	GWGCWC	2 cut(s)	364, 949
Alw26I	GTCTC	3 cut(s)	24, 870, 923
Alw44I	GTGCAC	1 cut(s)	360
AlwI	GGATC	3 cut(s)	12, 438, 632
Ama87I	CYCGRG	1 cut(s)	409
AoxI	GGCC	4 cut(s)	42, 178, 246, 969
ApaLI	GTGCAC	1 cut(s)	360
ApeKI	GCWGC	3 cut(s)	107, 125, 497
ApoI	RAATTY	2 cut(s)	562, 690
AspS9I	GGNCC	1 cut(s)	43
AsuHPI	GGTGA	4 cut(s)	320, 392, 890, 1043
AsuII	TTCGAA	1 cut(s)	991
AsuNHI	GCTAGC	1 cut(s)	281
AvaI	CYCGRG	1 cut(s)	409
BaeGI	GKGCMC	1 cut(s)	364
BalI	TGGCCA	2 cut(s)	248, 971
BamHI	GGATCC	1 cut(s)	4
Bbv12I	GWGCWC	2 cut(s)	364, 949
BbvI	GCAGC	3 cut(s)	94, 112, 484
BccI	CCATC	2 cut(s)	34, 380
BceAI	ACGGC	1 cut(s)	980
BclI	TGATCA	1 cut(s)	28
BcoDI	GTCTC	3 cut(s)	24, 870, 923
BfaI	CTAG	3 cut(s)	282, 482, 681
BfmI	CTRYAG	1 cut(s)	750
BfuAI	ACCTGC	1 cut(s)	773
BglI	GCCNNNNNGGC	1 cut(s)	92
BisI	GCNGC	3 cut(s)	108, 126, 498
BlsI	GCNGC	3 cut(s)	109, 127, 499
BmeT110I	CYCGRG	1 cut(s)	409
BmgT120I	GGNCC	1 cut(s)	43
BmiI	GGNNCC	2 cut(s)	6, 141
BmrI	ACTGGG	2 cut(s)	667, 859
BmsI	GCATC	2 cut(s)	832, 1006
BmtI	GCTAGC	1 cut(s)	285
BmuI	ACTGGG	2 cut(s)	667, 859
BpmI	CTGGAG	1 cut(s)	141
Bpu14I	TTCGAA	1 cut(s)	991
BsaI	GGTCTC	1 cut(s)	923
Bsc4I	CCNNNNNNNGG	1 cut(s)	956
Bse1I	ACTGG	3 cut(s)	662, 854, 982
Bse3DI	GCAATG	2 cut(s)	42, 299
BseGI	GGATG	3 cut(s)	374, 597, 847
BseLI	CCNNNNNNNGG	1 cut(s)	956
BseMI	GCAATG	2 cut(s)	42, 299
BseMII	CTCAG	1 cut(s)	35
BseNI	ACTGG	3 cut(s)	662, 854, 982
BseRI	GAGGAG	2 cut(s)	308, 770
BseSI	GKGCMC	1 cut(s)	364
BseXI	GCAGC	3 cut(s)	94, 112, 484
BseYI	CCCAGC	1 cut(s)	233
BshFI	GGCC	4 cut(s)	44, 180, 248, 971
BsiHKAI	GWGCWC	2 cut(s)	364, 949
BsiHKCI	CYCGRG	1 cut(s)	409
BslFI	GGGAC	3 cut(s)	182, 570, 1025
BslI	CCNNNNNNNGG	1 cut(s)	956
BsmAI	GTCTC	3 cut(s)	24, 870, 923
BsmFI	GGGAC	3 cut(s)	182, 570, 1025
BsnI	GGCC	4 cut(s)	44, 180, 248, 971
Bso31I	GGTCTC	1 cut(s)	923
BsoBI	CYCGRG	1 cut(s)	409
Bsp119I	TTCGAA	1 cut(s)	991
Bsp1286I	GDGCHC	2 cut(s)	364, 949
Bsp1407I	TGTACA	1 cut(s)	147
Bsp143I	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
BspACI	CCGC	1 cut(s)	548
BspANI	GGCC	4 cut(s)	44, 180, 248, 971
BspCNI	CTCAG	1 cut(s)	34
BspLI	GGNNCC	2 cut(s)	6, 141
BspMI	ACCTGC	1 cut(s)	773
BspOI	GCTAGC	1 cut(s)	285
BspPI	GGATC	3 cut(s)	12, 438, 632
BspQI	GCTCTTC	1 cut(s)	59
BspT104I	TTCGAA	1 cut(s)	991
BspTNI	GGTCTC	1 cut(s)	923
BsrDI	GCAATG	2 cut(s)	42, 299
BsrGI	TGTACA	1 cut(s)	147
BsrI	ACTGG	3 cut(s)	662, 854, 982
BssMI	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
Bst4CI	ACNGT	2 cut(s)	147, 211
Bst6I	CTCTTC	1 cut(s)	59
BstAPI	GCANNNNNTGC	2 cut(s)	842, 1025
BstAUI	TGTACA	1 cut(s)	147
BstBI	TTCGAA	1 cut(s)	991
BstC8I	GCNNGC	5 cut(s)	112, 231, 283, 834, 969
BstDEI	CTNAG	2 cut(s)	21, 907
BstENI	CCTNNNNNAGG	1 cut(s)	954
BstF5I	GGATG	3 cut(s)	374, 597, 847
BstKTI	GATC	8 cut(s)	7, 31, 314, 433, 532, 640, 816, 936
BstMAI	GTCTC	3 cut(s)	24, 870, 923
BstMBI	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 92, 116, 125, 842, 908, 1025
BstSFI	CTRYAG	1 cut(s)	750
BstSLI	GKGCMC	1 cut(s)	364
BstV1I	GCAGC	3 cut(s)	94, 112, 484
BstX2I	RGATCY	3 cut(s)	4, 430, 637
BstYI	RGATCY	3 cut(s)	4, 430, 637
BsuRI	GGCC	4 cut(s)	44, 180, 248, 971
BtsCI	GGATG	3 cut(s)	374, 597, 847
BtsIMutI	CAGTG	3 cut(s)	143, 847, 1051
BveI	ACCTGC	1 cut(s)	773
Cac8I	GCNNGC	5 cut(s)	112, 231, 283, 834, 969
Cfr13I	GGNCC	1 cut(s)	43
Csp6I	GTAC	5 cut(s)	148, 519, 526, 616, 976
CviAII	CATG	2 cut(s)	365, 862
CviQI	GTAC	5 cut(s)	148, 519, 526, 616, 976
DdeI	CTNAG	2 cut(s)	21, 907
DpnI	GATC	8 cut(s)	6, 30, 313, 432, 531, 639, 815, 935
DpnII	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
EaeI	YGGCCR	2 cut(s)	246, 969
Eam1104I	CTCTTC	1 cut(s)	59
EarI	CTCTTC	1 cut(s)	59
Eco31I	GGTCTC	1 cut(s)	923
Eco57I	CTGAAG	1 cut(s)	906
Eco88I	CYCGRG	1 cut(s)	409
EcoNI	CCTNNNNNAGG	1 cut(s)	954
EcoT22I	ATGCAT	1 cut(s)	1021
FaeI	CATG	2 cut(s)	368, 865
FaqI	GGGAC	3 cut(s)	182, 570, 1025
FatI	CATG	2 cut(s)	364, 861
FbaI	TGATCA	1 cut(s)	28
Fnu4HI	GCNGC	3 cut(s)	108, 126, 498
FokI	GGATG	3 cut(s)	381, 604, 854
Fsp4HI	GCNGC	3 cut(s)	108, 126, 498
FspBI	CTAG	3 cut(s)	282, 482, 681
GluI	GCNGC	3 cut(s)	108, 126, 498
GsaI	CCCAGC	1 cut(s)	237
GsuI	CTGGAG	1 cut(s)	141
HaeIII	GGCC	4 cut(s)	44, 180, 248, 971
Hin1II	CATG	2 cut(s)	368, 865
HinfI	GANTC	3 cut(s)	265, 758, 890
HphI	GGTGA	4 cut(s)	320, 392, 890, 1043
Hpy166II	GTNNAC	2 cut(s)	362, 762
Hpy188I	TCNGA	3 cut(s)	24, 316, 933
Hpy188III	TCNNGA	4 cut(s)	171, 254, 409, 533
Hpy8I	GTNNAC	2 cut(s)	362, 762
HpyAV	CCTTC	1 cut(s)	904
HpyCH4III	ACNGT	2 cut(s)	147, 211
HpyCH4V	TGCA	7 cut(s)	114, 304, 362, 513, 768, 954, 1019
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 92, 116, 125, 842, 908, 1025
HpyF3I	CTNAG	2 cut(s)	21, 907
Hsp92II	CATG	2 cut(s)	368, 865
Ksp22I	TGATCA	1 cut(s)	28
Kzo9I	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
LguI	GCTCTTC	1 cut(s)	59
LmnI	GCTCC	4 cut(s)	122, 278, 403, 952
Lsp1109I	GCAGC	3 cut(s)	94, 112, 484
LweI	GCATC	2 cut(s)	832, 1006
MaeI	CTAG	3 cut(s)	282, 482, 681
MalI	GATC	8 cut(s)	6, 30, 313, 432, 531, 639, 815, 935
MboI	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
MboII	GAAGA	6 cut(s)	76, 395, 647, 899, 1013, 1046
MfeI	CAATTG	3 cut(s)	102, 273, 486
MflI	RGATCY	3 cut(s)	4, 430, 637
MhlI	GDGCHC	2 cut(s)	364, 949
MlsI	TGGCCA	2 cut(s)	248, 971
MluCI	AATT	8 cut(s)	102, 258, 273, 486, 562, 690, 769, 993
MluNI	TGGCCA	2 cut(s)	248, 971
MlyI	GAGTC	1 cut(s)	767
MmeI	TCCRAC	1 cut(s)	685
Mox20I	TGGCCA	2 cut(s)	248, 971
Mph1103I	ATGCAT	1 cut(s)	1021
MscI	TGGCCA	2 cut(s)	248, 971
MseI	TTAA	2 cut(s)	417, 741
MslI	CAYNNNNRTG	3 cut(s)	367, 821, 873
Msp20I	TGGCCA	2 cut(s)	248, 971
MspA1I	CMGCKG	1 cut(s)	548
MunI	CAATTG	3 cut(s)	102, 273, 486
MwoI	GCNNNNNNNGC	7 cut(s)	41, 92, 116, 125, 842, 908, 1025
NdeII	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
NheI	GCTAGC	1 cut(s)	281
NlaIII	CATG	2 cut(s)	368, 865
NlaIV	GGNNCC	2 cut(s)	6, 141
NsiI	ATGCAT	1 cut(s)	1021
NspV	TTCGAA	1 cut(s)	991
PaeR7I	CTCGAG	1 cut(s)	409
PaqCI	CACCTGC	1 cut(s)	773
PciSI	GCTCTTC	1 cut(s)	59
PfeI	GAWTC	2 cut(s)	265, 890
PkrI	GCNGC	3 cut(s)	109, 127, 499
PleI	GAGTC	1 cut(s)	766
PpsI	GAGTC	1 cut(s)	766
PsiI	TTATAA	1 cut(s)	465
PspFI	CCCAGC	1 cut(s)	233
PspN4I	GGNNCC	2 cut(s)	6, 141
PspPI	GGNCC	1 cut(s)	43
PsuI	RGATCY	3 cut(s)	4, 430, 637
RsaI	GTAC	5 cut(s)	149, 520, 527, 617, 977
RsaNI	GTAC	5 cut(s)	148, 519, 526, 616, 976
RseI	CAYNNNNRTG	3 cut(s)	367, 821, 873
SapI	GCTCTTC	1 cut(s)	59
SaqAI	TTAA	2 cut(s)	417, 741
SatI	GCNGC	3 cut(s)	108, 126, 498
Sau3AI	GATC	8 cut(s)	4, 28, 311, 430, 529, 637, 813, 933
Sau96I	GGNCC	1 cut(s)	43
SchI	GAGTC	1 cut(s)	767
SduI	GDGCHC	2 cut(s)	364, 949
SfaNI	GCATC	2 cut(s)	832, 1006
SfcI	CTRYAG	1 cut(s)	750
Sfr274I	CTCGAG	1 cut(s)	409
SfuI	TTCGAA	1 cut(s)	991
SlaI	CTCGAG	1 cut(s)	409
SmiMI	CAYNNNNRTG	3 cut(s)	367, 821, 873
SmlI	CTYRAG	1 cut(s)	409
SmoI	CTYRAG	1 cut(s)	409
Sse9I	AATT	8 cut(s)	102, 258, 273, 486, 562, 690, 769, 993
SsiI	CCGC	1 cut(s)	548
SspMI	CTAG	3 cut(s)	282, 482, 681
TaaI	ACNGT	2 cut(s)	147, 211
TaqI	TCGA	3 cut(s)	48, 410, 991
TaqII	GACCGA	1 cut(s)	824
TasI	AATT	8 cut(s)	102, 258, 273, 486, 562, 690, 769, 993
TatI	WGTACW	2 cut(s)	147, 975
TfiI	GAWTC	2 cut(s)	265, 890
Tru1I	TTAA	2 cut(s)	417, 741
Tru9I	TTAA	2 cut(s)	417, 741
TscAI	CASTG	2 cut(s)	150, 854
TseI	GCWGC	3 cut(s)	107, 125, 497
TspDTI	ATGAA	2 cut(s)	842, 886
TspGWI	ACGGA	1 cut(s)	630
TspRI	CASTG	2 cut(s)	150, 854
VneI	GTGCAC	1 cut(s)	360
XagI	CCTNNNNNAGG	1 cut(s)	954
XapI	RAATTY	2 cut(s)	562, 690
XhoI	CTCGAG	1 cut(s)	409
XspI	CTAG	3 cut(s)	282, 482, 681
Zsp2I	ATGCAT	1 cut(s)	1021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.