pycom11g09270

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007649
Physical Location & Seq
Forward (+)
7441107 .. 7444937
3831 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGACTCGCCGGCCACCGACTCCATCACAAGATACGCATCTTTGCAAGAACCCGGTTGATGGATTCTCGGCCGGTCTGGTCGATGAAAACAATATCTTTGAGTGGAGCGTCACGATTATCGGACCGCCTGATACGCTCTACGAGGGAGGATTTTTCAATGCCATCATGAGCTTTCCTAATAATTATCCAAACAGCCCTCCGACGGTTAAGTTTACTTCGGAGATATGGCATCCGAATGTTTATACTGACGGGCGTGTTTGCATATCTATCCTTCACCCGCCTGGTGAGGACCCAAATGGTTACGAGCTTGCAAGTGAACGCTGGACGCCTGTCCATACGGTTGAAAGCATAGTCTTGAGTATAATATCGATGCTATCCAGTCCAAATGACGAATCTCCTGCAAATGTTGAAGCCGCTAAGGAGTGGAGAGACCGGAGAGAGGATTTCAAGAAAAAGGTCAGCCGCTGTGTGAGGAAATCACAAGAGATGCTATGA

Protein Analysis

165

Amino Acids

18.52

Weight (kDa)

5.2

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 13 - 156 2e-50 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 125, 278, 414, 463
AcoI YGGCCR 2 cut(s) 11, 69
AcyI GRCGYC 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 59, 202, 439
AgsI TTSAA 4 cut(s) 157, 344, 410, 448
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 2 cut(s) 171, 307
AluI AGCT 2 cut(s) 171, 307
Alw26I GTCTC 1 cut(s) 423
AlwNI CAGNNNCTG 1 cut(s) 465
AoxI GGCC 2 cut(s) 11, 69
AspS9I GGNCC 2 cut(s) 122, 289
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 266, 296
AvaII GGWCC 2 cut(s) 122, 289
BccI CCATC 3 cut(s) 31, 53, 170
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 1 cut(s) 282
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 423
BisI GCNGC 2 cut(s) 414, 463
BlsI GCNGC 2 cut(s) 415, 464
Bme1390I CCNGG 2 cut(s) 53, 282
Bme18I GGWCC 2 cut(s) 122, 289
BmgT120I GGNCC 2 cut(s) 122, 289
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 2 cut(s) 53, 282
BmsI GCATC 4 cut(s) 46, 238, 360, 477
BoxI GACNNNNGTC 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 376
BpuMI CCSGG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 423
BsaWI WCCGGW 1 cut(s) 432
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 202, 439
Bse118I RCCGGY 2 cut(s) 9, 71
Bse1I ACTGG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 368
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 3 cut(s) 59, 202, 439
BseNI ACTGG 1 cut(s) 378
BseX3I CGGCCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 13, 71
BshVI ATCGAT 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 72
BsiSI CCGG 4 cut(s) 10, 53, 72, 433
BslI CCNNNNNNNGG 3 cut(s) 59, 202, 439
BsmAI GTCTC 1 cut(s) 423
BsnI GGCC 2 cut(s) 13, 71
Bso31I GGTCTC 1 cut(s) 423
BspACI CCGC 4 cut(s) 125, 278, 414, 463
BspANI GGCC 2 cut(s) 13, 71
BspDI ATCGAT 1 cut(s) 368
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 423
BsrFI RCCGGY 2 cut(s) 9, 71
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 2 cut(s) 9, 71
BssNI GRCGYC 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 205, 340
BstACI GRCGYC 1 cut(s) 326
BstC8I GCNNGC 2 cut(s) 11, 309
BstDEI CTNAG 1 cut(s) 417
BstF5I GGATG 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 423
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 282
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 51, 280
BstZI CGGCCG 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 368
BsuRI GGCC 2 cut(s) 13, 71
BsuTUI ATCGAT 1 cut(s) 368
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 2 cut(s) 11, 309
CaiI CAGNNNCTG 1 cut(s) 465
CciI TCATGA 1 cut(s) 165
Cfr10I RCCGGY 2 cut(s) 9, 71
Cfr13I GGNCC 2 cut(s) 122, 289
ClaI ATCGAT 1 cut(s) 368
CpoI CGGWCCG 1 cut(s) 122
CseI GACGC 2 cut(s) 97, 334
CspI CGGWCCG 1 cut(s) 122
CviAII CATG 1 cut(s) 166
CviJI RGCY 7 cut(s) 13, 71, 171, 195, 307, 413, 462
CviKI_1 RGCY 7 cut(s) 13, 71, 171, 195, 307, 413, 462
DdeI CTNAG 1 cut(s) 417
EaeI YGGCCR 2 cut(s) 11, 69
EagI CGGCCG 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 122, 289
Eco52I CGGCCG 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 280
FaeI CATG 1 cut(s) 169
FaiI YATR 8 cut(s) 167, 226, 243, 263, 336, 350, 362, 493
FatI CATG 1 cut(s) 165
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 2 cut(s) 414, 463
FokI GGATG 1 cut(s) 216
Fsp4HI GCNGC 2 cut(s) 414, 463
GluI GCNGC 2 cut(s) 414, 463
HaeIII GGCC 2 cut(s) 13, 71
HapII CCGG 4 cut(s) 10, 53, 72, 433
HgaI GACGC 2 cut(s) 97, 334
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 1 cut(s) 169
HinfI GANTC 4 cut(s) 4, 19, 63, 392
HpaII CCGG 4 cut(s) 10, 53, 72, 433
HphI GGTGA 2 cut(s) 266, 296
Hpy166II GTNNAC 2 cut(s) 213, 317
Hpy188I TCNGA 4 cut(s) 122, 201, 220, 234
Hpy188III TCNNGA 4 cut(s) 112, 166, 355, 448
Hpy8I GTNNAC 2 cut(s) 213, 317
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 2 cut(s) 205, 340
HpyCH4V TGCA 4 cut(s) 45, 261, 311, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 1 cut(s) 169
KroI GCCGGC 1 cut(s) 9
KroNI GCCGGC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 105
LweI GCATC 4 cut(s) 46, 238, 360, 477
MaeIII GTNAC 2 cut(s) 109, 299
MluCI AATT 1 cut(s) 181
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 6 cut(s) 136, 140, 207, 280, 433, 465
MroNI GCCGGC 1 cut(s) 9
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 465
MspI CCGG 4 cut(s) 10, 53, 72, 433
MspR9I CCNGG 2 cut(s) 53, 282
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 133
NaeI GCCGGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 53
NgoMIV GCCGGC 1 cut(s) 9
NlaIII CATG 1 cut(s) 169
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 109
PagI TCATGA 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 63, 392
PkrI GCNGC 2 cut(s) 415, 464
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 289
PshAI GACNNNNGTC 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 2 cut(s) 122, 289
PspPPI RGGWCCY 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 465
RseI CAYNNNNRTG 1 cut(s) 234
Rsr2I CGGWCCG 1 cut(s) 122
RsrII CGGWCCG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 2 cut(s) 414, 463
Sau96I GGNCC 2 cut(s) 122, 289
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 53, 282
SetI ASST 3 cut(s) 173, 309, 459
SfaNI GCATC 4 cut(s) 46, 238, 360, 477
SinI GGWCC 2 cut(s) 122, 289
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 1 cut(s) 181
SsiI CCGC 4 cut(s) 125, 278, 414, 463
StyD4I CCNGG 2 cut(s) 51, 280
TaaI ACNGT 2 cut(s) 205, 340
TaqI TCGA 2 cut(s) 81, 368
TasI AATT 1 cut(s) 181
TauI GCSGC 2 cut(s) 416, 465
TfiI GAWTC 2 cut(s) 63, 392
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 99
VpaK11BI GGWCC 2 cut(s) 122, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.