pycom11g09820

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007320
Physical Location & Seq
Forward (+)
8177094 .. 8184554
7461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2454 bp
ATGGACGACGAATTGCTGGAACTGCAGAGGCAATTCGAGTTCGCACAGCAGGCGAAATCGAGCATCAGATTATCCGATCGAAATGTCGTCGAATTGGTCCAGAAGCTTCAGGAGCTGCATATCATCGACTTCGAGCTTCTCCACACCGTCACCGGCAAAGAATACATTACTCCCGATCAATTGAGGAATGAGATATCGGCGGAGGTCAGTAAACTAGGGCGTGTTTCGTTGATTGACCTGGCGGACGCTACCGGAGTTGATTTGTATCATGTTGAGAAGCAAGCTCAACATGTTGTTTCGGATGATCTGGGGCTTATGTTGATTCAAGGGGAAATAATATCGCAGTCTTATTGGGATTCTGTCGCCGAAGAAGTCAATGACAGGCTTCAAGAGTGTAGCCAAATCGCTCTGGCAGAACTTGCCGCGCAGTTGCATGTCAGTTCGGAAATGGTGGCATCTGTGTTGGAGCCCCGCCTTGGGACTTTGGTGAAAGGTAGGCTTGAAGGTGGGCAATTGTATACACCTGCATATGTTGCACGGGTTACTGCTATGGTTCGTGGTGCTGCCAGAGGTATCGCAGTTCCCACAAACTTATCATTGTTGTGGAGTTCTTTACAGCAGTTGCTGCAAGAAATGGATGGAGGGAGTGGAGTGGCTGTAGAAGGTTCTTTTTTCCAGTCCCTATTTAATGGGCTTGTAAGGGAAGGACAGCTTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCTAATGTCTTTGCCATTGCTCAAAAGGAATCAATTGATTCTTTCTTTTCACAGAATTCTTTCATTAACTATGATGTTCTGCACAAACTTAGAATTCCTCAGCCTATTCAGTTCTTGCAGTCCAGATATCCAGAAGGCATACCTTTGGTTACTACATTTGTTCACCCCTCAATGATTGAGATGCTTGATGCTGCTACGGAAGATGCTCTCGAACGTGATAGCTGGATCGATTCTCTTTCTATACTGCCGATGTCCTTTGGGTCTCAAGATGCATCTAAACTTTTGTCCCTCTGCCCTTCCATTCAGCAGGGTCTCAAGTCTGATAAAGCAATACTATTCGGGGACTCGTATGTGTTTAGCAGTGGCTTTATCAAGGATGTGTATGATCTCCTAGAGAAAGAAATGGAAACCTTTAGTGTTTCAGTTCCTTCCAGTACTGTGGTGTCAGATGATTTGCATGAGACAAAAATTGGTCATGACACAAGCAGGTCGACTGCGTCAAATGAAACTGTTAGTGACAGCAGCAGTAATAAACAGGCAACAGAGAAAGGATCAAAAAAGAAAAAAGGTAGAGGGGCTGGGAACATGATGACAGGGCCAGCTGAGAGTGAGCTGGACAACCAGGACAGTGTTCCTACGAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATATCTCTTCCGGGCAGGCTGCAGAGTCAAAAGCAGCTGCTAAATTGGTGAAAATAAAAGAGGAAAATCTCAACGTCCCTTCAGAAGATTGGGTAATGAATAAGATTACAGCGCTAGTACCTGATTTTGAAGAACAAGGTCTTGACGGTCCCCACACAATTCTTGGACCTTTGGCACACTATTTGAGGCCTAAGTTAATCAACTCCTGGAAGGAGAGAAGAAAGGCATTGTTTACAGAAAATGCGGAGAGAATGAAGAACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAATATGCAGCTTTATGAAAAGGCTTTAGATTTGTTTGAAGATGACCAATCAACCTCAGTCATTTTTCATCGGCATCTGTTAAGAACAACAGCTACTACCATTGCTGATATACTTCTTCACAATTTGGACATGCACAACAAATTGAAGAATGGAGTCGAAGAAGTTGCCGAGCCTCAAATTTCAGAATCTATTTCCCTCAATGCGGGAGAGAGAACTTCCATTGCCAAGAATTTTCCTGGATCTCTTTCAATCAAGGCTCTTGCTGTGGTTGAAGCATTGGAAGGAAAGCGGGTGGAAACATTCATGACTGCTCTAAGAGATATTGCAGAAGAGAGTGGCTTGCTCTTGAAAAAGCTTGACAAGAAACTGGAAAGAACACTTCTGCATTCATATCAGAAGGATTTGGTGTCTCAAGTTTCTGCTGAGACCGATCCAGTTCCTCTTTTGCCAAAAGTTGTTTCCCTGATTTACGTTCAGGTTCACCATAAAGCTCTTCAAGCACCTGGAAGGGCCATCGCTGTTGCTGTTTCACGGCTGAAGGATAAACTGGATGAATCAGCCTTCAAGATCTTGACAGATTATCAAACTACAACAGTGACCCTGTTAACGTTAATATCTGGTGCAAGTGGAGAGGAAGAAGATTGTTCATCTGATAGAATTTTGAGCAAAAGGGAGCTTTTGGAGACTCAAATGCCTGCACTCAAAGGCCTGGTCGTACGCACCTCACAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

818

Amino Acids

90.35

Weight (kDa)

5.35

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 4 - 288 4.3e-97 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 305 - 352 1.4e-07 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 561 - 692 3.6e-38 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 697 - 810 3.6e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 2 cut(s) 532, 1224
AccI GTMKAC 2 cut(s) 518, 1238
AccII CGCG 1 cut(s) 425
AciI CCGC 7 cut(s) 200, 242, 423, 472, 1661, 1922, 2008
AclI AACGTT 1 cut(s) 2327
AclWI GGATC 5 cut(s) 727, 980, 1306, 1966, 2144
AcsI RAATTY 5 cut(s) 802, 840, 1896, 1948, 2376
AcuI CTGAAG 3 cut(s) 92, 1482, 2276
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 3 cut(s) 1183, 1536, 2436
AfeI AGCGCT 1 cut(s) 1530
AfiI CCNNNNNNNGG 3 cut(s) 476, 477, 1921
AflIII ACRYGT 1 cut(s) 289
AjnI CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
AjuI GAANNNNNNNTTGG 2 cut(s) 2161, 2193
Alw26I GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
AlwI GGATC 5 cut(s) 727, 980, 1306, 1966, 2144
AlwNI CAGNNNCTG 2 cut(s) 115, 625
Aor51HI AGCGCT 1 cut(s) 1530
AoxI GGCC 4 cut(s) 1343, 1604, 2229, 2425
ApeKI GCWGC 9 cut(s) 115, 563, 625, 938, 1269, 1436, 1451, 1454, 1726
ApoI RAATTY 5 cut(s) 802, 840, 1896, 1948, 2376
ArsI GACNNNNNNTTYG 2 cut(s) 2415, 2447
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 2 cut(s) 427, 1531
AspS9I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 5 cut(s) 142, 499, 902, 1477, 2192
AvaII GGWCC 3 cut(s) 97, 1565, 1583
BanII GRGCYC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1423, 1441, 1463, 1738
BccI CCATC 2 cut(s) 632, 2240
BceAI ACGGC 1 cut(s) 2267
BcgI CGANNNNNNTGC 2 cut(s) 1865, 1899
BciT130I CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BfaI CTAG 3 cut(s) 215, 1139, 1532
BfmI CTRYAG 3 cut(s) 23, 657, 1437
BfoI RGCGCY 1 cut(s) 1532
BfuAI ACCTGC 2 cut(s) 532, 1224
BglII AGATCT 1 cut(s) 2286
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
Bme18I GGWCC 3 cut(s) 97, 1565, 1583
BmgT120I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
BmiI GGNNCC 2 cut(s) 468, 1567
BmrFI CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
BmsI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
Bpu10I CCTNAGC 1 cut(s) 846
BpuEI CTTGAG 3 cut(s) 996, 1046, 2115
BpuMI CCSGG 1 cut(s) 1429
Bsa29I ATCGAT 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 3 cut(s) 1014, 1064, 2138
BsaJI CCNNGG 1 cut(s) 475
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 477, 1921
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 5 cut(s) 676, 1179, 2091, 2153, 2271
Bse3DI GCAATG 3 cut(s) 762, 1818, 1938
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 4 cut(s) 307, 643, 1129, 2275
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 476, 477, 1921
BseMI GCAATG 3 cut(s) 762, 1818, 1938
BseMII CTCAG 4 cut(s) 860, 1341, 1788, 2133
BseNI ACTGG 5 cut(s) 676, 1179, 2091, 2153, 2271
BseXI GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1423, 1441, 1463, 1738
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 3 cut(s) 750, 812, 2400
Bsh1236I CGCG 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 4 cut(s) 1345, 1606, 2231, 2427
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 79
BsiSI CCGG 3 cut(s) 153, 252, 1428
BsiWI CGTACG 1 cut(s) 2434
BslFI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
BslI CCNNNNNNNGG 3 cut(s) 476, 477, 1921
BsmAI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BsmFI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
BsmI GAATGC 1 cut(s) 2104
BsnI GGCC 4 cut(s) 1345, 1606, 2231, 2427
Bso31I GGTCTC 3 cut(s) 1014, 1064, 2138
Bsp1286I GDGCHC 1 cut(s) 471
BspACI CCGC 7 cut(s) 200, 242, 423, 472, 1661, 1922, 2008
BspANI GGCC 4 cut(s) 1345, 1606, 2231, 2427
BspCNI CTCAG 4 cut(s) 859, 1342, 1787, 2134
BspDI ATCGAT 1 cut(s) 975
BspFNI CGCG 1 cut(s) 425
BspHI TCATGA 2 cut(s) 1222, 2022
BspLI GGNNCC 2 cut(s) 468, 1567
BspMAI CTGCAG 2 cut(s) 27, 1441
BspMI ACCTGC 2 cut(s) 532, 1224
BspPI GGATC 5 cut(s) 727, 980, 1306, 1966, 2144
BspQI GCTCTTC 1 cut(s) 2217
BspTNI GGTCTC 3 cut(s) 1014, 1064, 2138
BsrDI GCAATG 3 cut(s) 762, 1818, 1938
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 5 cut(s) 676, 1179, 2091, 2153, 2271
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 475
BssNAI GTATAC 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 475
Bst1107I GTATAC 1 cut(s) 519
Bst2UI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
Bst4CI ACNGT 6 cut(s) 148, 1186, 1258, 1376, 1565, 2314
Bst6I CTCTTC 3 cut(s) 1429, 2043, 2217
BstAPI GCANNNNNTGC 3 cut(s) 419, 533, 625
BstC8I GCNNGC 6 cut(s) 51, 282, 1347, 1434, 2060, 2415
BstDEI CTNAG 8 cut(s) 836, 846, 1350, 1392, 1608, 1774, 2033, 2142
BstF5I GGATG 4 cut(s) 307, 643, 1129, 2275
BstFNI CGCG 1 cut(s) 425
BstH2I RGCGCY 1 cut(s) 1532
BstHHI GCGC 2 cut(s) 427, 1531
BstMAI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
BstNI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BstNSI RCATGY 3 cut(s) 293, 437, 1852
BstSCI CCNGG 7 cut(s) 237, 1368, 1427, 1622, 1954, 2221, 2427
BstSFI CTRYAG 3 cut(s) 23, 657, 1437
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1423, 1441, 1463, 1738
BstX2I RGATCY 2 cut(s) 1958, 2286
BstXI CCANNNNNNTGG 1 cut(s) 1186
BstYI RGATCY 2 cut(s) 1958, 2286
BstZ17I GTATAC 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 975
BsuRI GGCC 4 cut(s) 1345, 1606, 2231, 2427
BsuTUI ATCGAT 1 cut(s) 975
BtgZI GCGATG 1 cut(s) 2218
BtsCI GGATG 4 cut(s) 307, 643, 1129, 2275
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 3 cut(s) 1114, 1381, 2319
BveI ACCTGC 2 cut(s) 532, 1224
Cac8I GCNNGC 6 cut(s) 51, 282, 1347, 1434, 2060, 2415
CaiI CAGNNNCTG 2 cut(s) 115, 625
CciI TCATGA 2 cut(s) 1222, 2022
CfoI GCGC 2 cut(s) 427, 1531
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 2 cut(s) 254, 1233
Csp6I GTAC 3 cut(s) 1182, 1535, 2435
CviAII CATG 8 cut(s) 269, 290, 434, 1205, 1223, 1333, 1849, 2023
CviQI GTAC 3 cut(s) 1182, 1535, 2435
DdeI CTNAG 8 cut(s) 836, 846, 1350, 1392, 1608, 1774, 2033, 2142
DraIII CACNNNGTG 1 cut(s) 728
Eam1104I CTCTTC 3 cut(s) 1429, 2043, 2217
EarI CTCTTC 3 cut(s) 1429, 2043, 2217
EciI GGCGGA 2 cut(s) 215, 257
Eco130I CCWWGG 1 cut(s) 475
Eco147I AGGCCT 2 cut(s) 1606, 2427
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 3 cut(s) 1014, 1064, 2138
Eco32I GATATC 2 cut(s) 195, 875
Eco47I GGWCC 3 cut(s) 97, 1565, 1583
Eco47III AGCGCT 1 cut(s) 1530
Eco57I CTGAAG 3 cut(s) 92, 1482, 2276
EcoRI GAATTC 2 cut(s) 802, 840
EcoRII CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
EcoRV GATATC 2 cut(s) 195, 875
EcoT14I CCWWGG 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 8 cut(s) 272, 293, 437, 1208, 1226, 1336, 1852, 2026
FalI AAGNNNNNCTT 4 cut(s) 483, 515, 696, 728
FaqI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
FatI CATG 8 cut(s) 268, 289, 433, 1204, 1222, 1332, 1848, 2022
FauI CCCGC 3 cut(s) 479, 1915, 2001
FauNDI CATATG 1 cut(s) 529
FblI GTMKAC 2 cut(s) 518, 1238
FokI GGATG 4 cut(s) 314, 650, 1136, 2282
FriOI GRGCYC 1 cut(s) 471
FspBI CTAG 3 cut(s) 215, 1139, 1532
GlaI GCGC 2 cut(s) 426, 1530
GsaI CCCAGC 1 cut(s) 1329
HaeII RGCGCY 1 cut(s) 1532
HaeIII GGCC 4 cut(s) 1345, 1606, 2231, 2427
HapII CCGG 3 cut(s) 153, 252, 1428
HgaI GACGC 2 cut(s) 254, 1233
HhaI GCGC 2 cut(s) 427, 1531
Hin1II CATG 8 cut(s) 272, 293, 437, 1208, 1226, 1336, 1852, 2026
Hin6I GCGC 2 cut(s) 425, 1529
HinP1I GCGC 2 cut(s) 425, 1529
HincII GTYRAC 2 cut(s) 1239, 2325
HindII GTYRAC 2 cut(s) 1239, 2325
HindIII AAGCTT 2 cut(s) 104, 2072
HpaI GTTAAC 1 cut(s) 2325
HpaII CCGG 3 cut(s) 153, 252, 1428
HphI GGTGA 5 cut(s) 142, 499, 902, 1477, 2192
Hpy166II GTNNAC 7 cut(s) 212, 519, 910, 1239, 1650, 2200, 2325
Hpy8I GTNNAC 7 cut(s) 212, 519, 910, 1239, 1650, 2200, 2325
Hpy99I CGWCG 2 cut(s) 11, 92
HpyCH4III ACNGT 6 cut(s) 148, 1186, 1258, 1376, 1565, 2314
HpyCH4IV ACGT 4 cut(s) 961, 1491, 2190, 2327
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
HpyF3I CTNAG 8 cut(s) 836, 846, 1350, 1392, 1608, 1774, 2033, 2142
HpySE526I ACGT 4 cut(s) 961, 1491, 2190, 2327
Hsp92II CATG 8 cut(s) 272, 293, 437, 1208, 1226, 1336, 1852, 2026
HspAI GCGC 2 cut(s) 425, 1529
KspAI GTTAAC 1 cut(s) 2325
LguI GCTCTTC 1 cut(s) 2217
LmnI GCTCC 3 cut(s) 112, 466, 2392
Lsp1109I GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1423, 1441, 1463, 1738
LweI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
MaeI CTAG 3 cut(s) 215, 1139, 1532
MaeII ACGT 4 cut(s) 961, 1491, 2190, 2327
MaeIII GTNAC 5 cut(s) 148, 541, 895, 1262, 2314
MfeI CAATTG 3 cut(s) 179, 512, 780
MflI RGATCY 2 cut(s) 1958, 2286
MhlI GDGCHC 1 cut(s) 471
MlyI GAGTC 5 cut(s) 1085, 1451, 1718, 1881, 2398
MmeI TCCRAC 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 1021
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
MslI CAYNNNNRTG 1 cut(s) 601
MspA1I CMGCKG 2 cut(s) 1349, 1454
MspI CCGG 3 cut(s) 153, 252, 1428
MspR9I CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
MunI CAATTG 3 cut(s) 179, 512, 780
Mva1269I GAATGC 1 cut(s) 2104
MvaI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
NciI CCSGG 1 cut(s) 1429
NdeI CATATG 1 cut(s) 529
NlaIII CATG 8 cut(s) 272, 293, 437, 1208, 1226, 1336, 1852, 2026
NlaIV GGNNCC 2 cut(s) 468, 1567
NmeAIII GCCGAG 1 cut(s) 1912
NmuCI GTSAC 3 cut(s) 148, 1262, 2314
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 3 cut(s) 293, 437, 1852
PagI TCATGA 2 cut(s) 1222, 2022
PaqCI CACCTGC 1 cut(s) 532
PceI AGGCCT 2 cut(s) 1606, 2427
PciI ACATGT 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 2217
PctI GAATGC 1 cut(s) 2104
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 7 cut(s) 322, 356, 776, 785, 977, 1904, 2273
Pfl23II CGTACG 1 cut(s) 2434
PflFI GACNNNGTC 1 cut(s) 1243
PfoI TCCNGGA 2 cut(s) 1622, 1954
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 5 cut(s) 1085, 1450, 1717, 1880, 2398
PpsI GAGTC 5 cut(s) 1085, 1450, 1717, 1880, 2398
PscI ACATGT 1 cut(s) 289
Psp1406I AACGTT 1 cut(s) 2327
Psp6I CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
PspLI CGTACG 1 cut(s) 2434
PspN4I GGNNCC 2 cut(s) 468, 1567
PspPI GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
PsrI GAACNNNNNNTAC 2 cut(s) 1795, 1827
PstI CTGCAG 2 cut(s) 27, 1441
PstNI CAGNNNCTG 2 cut(s) 115, 625
PsuI RGATCY 2 cut(s) 1958, 2286
PsyI GACNNNGTC 1 cut(s) 1243
PvuI CGATCG 1 cut(s) 79
PvuII CAGCTG 2 cut(s) 1349, 1454
RsaI GTAC 3 cut(s) 1183, 1536, 2436
RsaNI GTAC 3 cut(s) 1182, 1535, 2435
RseI CAYNNNNRTG 1 cut(s) 601
SalI GTCGAC 1 cut(s) 1237
SapI GCTCTTC 1 cut(s) 2217
SaqAI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
Sau96I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
ScaI AGTACT 1 cut(s) 1183
SchI GAGTC 5 cut(s) 1085, 1451, 1718, 1881, 2398
ScrFI CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
SfcI CTRYAG 3 cut(s) 23, 657, 1437
SinI GGWCC 3 cut(s) 97, 1565, 1583
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 3 cut(s) 1011, 1061, 2130
SmoI CTYRAG 3 cut(s) 1011, 1061, 2130
SseBI AGGCCT 2 cut(s) 1606, 2427
SsiI CCGC 7 cut(s) 200, 242, 423, 472, 1661, 1922, 2008
SspMI CTAG 3 cut(s) 215, 1139, 1532
StuI AGGCCT 2 cut(s) 1606, 2427
StyD4I CCNGG 7 cut(s) 237, 1368, 1427, 1622, 1954, 2221, 2427
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 6 cut(s) 148, 1186, 1258, 1376, 1565, 2314
TaiI ACGT 4 cut(s) 964, 1494, 2193, 2330
TaqII GACCGA 1 cut(s) 2162
TatI WGTACW 1 cut(s) 1181
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 7 cut(s) 322, 356, 776, 785, 977, 1904, 2273
Tru1I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
Tru9I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
TscAI CASTG 3 cut(s) 1114, 1381, 2319
TseFI GTSAC 3 cut(s) 148, 1262, 2314
TseI GCWGC 9 cut(s) 115, 563, 625, 938, 1269, 1436, 1451, 1454, 1726
Tsp45I GTSAC 3 cut(s) 148, 1262, 2314
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 3 cut(s) 1114, 1381, 2319
Tth111I GACNNNGTC 1 cut(s) 1243
VpaK11BI GGWCC 3 cut(s) 97, 1565, 1583
XapI RAATTY 5 cut(s) 802, 840, 1896, 1948, 2376
XceI RCATGY 3 cut(s) 293, 437, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XmiI GTMKAC 2 cut(s) 518, 1238
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 3 cut(s) 215, 1139, 1532
ZrmI AGTACT 1 cut(s) 1183
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.