Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0008670

Physical Location & Seq

Genomic Coordinates Reverse (-)

8198545 .. 8203311

Gene Structure 4767 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGCCAGGTACATTAACATCAAGAAACTCAACAATAAATAATGTTCCTTCTGGTGGAGTCCAACAACCAGCTGGAAGCCTCTCTGGCGGACGATTCACATCAAATAACCTTCCTGTTGCTTTGTCTCAGTTATCTCATGGAAGCTCCCATGCACATTCAGGAGTCACTAATAGAGGAGGATTGGGCGTGTCCCCAATTTTGGGAAATGCAGGTCCTCGGATTACAAGTTCCATGGGAAACATGGTTGGTGGGGGAAACATTGGGAGGAGTATTAGCACTGGTGGAGGCTTGTCGGTGCCTGGTCTTGCTTCTCGTCTGAATTTAAGTGGAAATAGTGGATCTGGAAGTTTAACTGTACAAGGGCAAAATCGCTTGATGAGTGGTGTGCTACCACAAGGGTCTCCGCAAGTAATTTCGATGCTAGGAAATTCTTACCCTAATGCTGGTGGTCCACTTTCTCAAAGCCATGTCCAAGTGAATAATTTGAGCTCGATGGGAATAATGAATGATGTGAATTCTAATGACAGTTCTCCTTTTGACATCAATGATTTCCCTTCATTAACAAGTCGTCCTAGTTCTGCGGGAGGGCCTCAGGGGCAGTTGGGTTCACTACGAAAACAAGGCCTTGGTGTTAGTCCTATTGTTCAACAGAACCAAGAGTTCAGCATCCAAAATGAGGACTTTCCAGCTTTACCAGGATTTAAAGCAGGTGGTGATGCTGAATATGGGATCAATATGCACCAGAAAGAACAACTCCATGACAATGCTGTGTCGATGATGCAATCTCAGCACTTCCCAATGGGACGGTCTTCAGGATTTAACTTGGGGGGAACTTATTCTTCTCATAGGCCACAACAGCAACAACATGCTCCATCAGTCAGCAGTAGTGGTGTCTCGTTTTCACAAGTAAACAATCAGGATCTTCTCCATATGCATGGTTCAGACATATTCCCATCATCACATTCAACATATCATTCACAGACTAGTGGAGGACCTCCTGGCATTGGATTAAGGCCTCTGAATTCTGCCAATTCAGTTTCTGGTATGGGTTCATATGACCAGCTTATTCAGCAGTATCAACAACAAAATCAATCCCAGTTTCGCCTGCACCAGATGTCAGCTGGCAATCAATCATTTAGGGATCAGGGAATGAAATCGATGCAGTCAGCGCAATCTGCTCCAGACCTATTTGGTTTGCTTGGATTGTTAAGTGTCATAAGGATGAGTGATCCTGATCTTACATCCCTTGCACTTGGAATTGATTTGACAACACTTGGATTAAACTTGAATTCAACAGAAAACCTTCACAAGACTTTTGGTTCCCCATGGTCCGACGAGCCAGCTAAGGGTGATCCAGAGTTTAGTGTTCCACAATGTTACTATGCTAAACAACCGCCTGCACTACATCAAGGGTATTTTTCAAAGTTTTCCGTGGAAACGTTGTTCTATATCTTTTACAGCATGCCCAAAGACGAGGCCCAATTATATGCTGCCAATGAACTCAATAATAGAGGCTGGTTTTATCACAAGGAACACCGCCTATGGTTCATAAGAGTACCCAACATGGAGCCACTTGTGAAGACCAACACGTTTGAGAGAGGATCGATTATCACTGCTTTGATCCCAATACCTTTGAAACTATCCGAAAGGATAATTTTCTTGTGCATTACGAGTTGTTGGAGAAGAGACCGGTGTTACCACAGCATTGACGGTCTTAATATTTTTAGAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000288 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006355 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0015074 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903506 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

NOT2_3_5

Protein Analysis

578

Amino Acids

61.99

Weight (kDa)

8.29

Isoelectric Point (pI)

56.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOT2_3_5_C	PF04153	436 - 535	3.2e-32	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07705 AT1G07705
fragaria_vesca	FvH4_3g34620 FvH4_3g34620 FvH4_3g34620
malus_domestica	MD03G1103300.v1.1 MD11G1117100.v1.1
prunus_persica	Prupe.6G087300_v2.0.a1
pyrus_communis	pycom03g08390 pycom11g09830
rosa_chinensis	RchiOBHm_Chr5g0062331
rosa_laevigata	RLG00000035550
rosa_multiflora	Rmu_sc0006295.1_g000002 Rmu_ssc0000409.1_g000034
rosa_roxburghii	Rroxscaffold_1G00018350
rosa_rugosa	Rorug05G0348500 Rorug05G0348600 Rorug05G0348700
rosa_samantha	Rh5AG408400 Rh5BG422100 Rh5CG446100 Rh5DG437100
rosa_wichuraiana	Rw5G038480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	698
Acc36I	ACCTGC	2 cut(s)	200, 698
AccB1I	GGYRCC	1 cut(s)	295
AciI	CCGC	5 cut(s)	87, 404, 581, 1394, 1537
AclI	AACGTT	1 cut(s)	1439
AclWI	GGATC	8 cut(s)	346, 737, 927, 1149, 1223, 1346, 1609, 1615
AcsI	RAATTY	5 cut(s)	319, 427, 514, 1021, 1288
AcuI	CTGAAG	1 cut(s)	795
AfaI	GTAC	3 cut(s)	10, 357, 1557
AfiI	CCNNNNNNNGG	7 cut(s)	53, 199, 200, 443, 676, 1004, 1346
AflIII	ACRYGT	1 cut(s)	1587
AgeI	ACCGGT	1 cut(s)	1689
AgsI	TTSAA	6 cut(s)	647, 966, 1288, 1293, 1422, 1636
AhlI	ACTAGT	1 cut(s)	983
AjnI	CCWGG	4 cut(s)	4, 298, 694, 997
AloI	GAACNNNNNNTCC	4 cut(s)	211, 243, 1427, 1459
AluBI	AGCT	7 cut(s)	71, 144, 489, 689, 1063, 1121, 1343
AluI	AGCT	7 cut(s)	71, 144, 489, 689, 1063, 1121, 1343
Alw21I	GWGCWC	1 cut(s)	491
Alw26I	GTCTC	4 cut(s)	129, 405, 898, 1680
AlwI	GGATC	8 cut(s)	346, 737, 927, 1149, 1223, 1346, 1609, 1615
AlwNI	CAGNNNCTG	1 cut(s)	1040
AoxI	GGCC	5 cut(s)	587, 622, 848, 1013, 1476
ApeKI	GCWGC	1 cut(s)	1490
ApoI	RAATTY	5 cut(s)	319, 427, 514, 1021, 1288
AsiGI	ACCGGT	1 cut(s)	1689
Asp700I	GAANNNNTTC	2 cut(s)	835, 1302
AspLEI	GCGC	1 cut(s)	1171
AspS9I	GGNCC	6 cut(s)	212, 449, 587, 992, 1329, 1477
AsuHPI	GGTGA	2 cut(s)	725, 1361
AvaII	GGWCC	4 cut(s)	212, 449, 992, 1329
AxyI	CCTNAGG	1 cut(s)	591
BanI	GGYRCC	1 cut(s)	295
BanII	GRGCYC	1 cut(s)	491
BbsI	GAAGAC	2 cut(s)	801, 1586
Bbv12I	GWGCWC	1 cut(s)	491
BbvI	GCAGC	1 cut(s)	1477
BccI	CCATC	3 cut(s)	487, 880, 961
BciT130I	CCWGG	4 cut(s)	6, 300, 696, 999
BcoDI	GTCTC	4 cut(s)	129, 405, 898, 1680
BcuI	ACTAGT	1 cut(s)	983
BfaI	CTAG	3 cut(s)	422, 573, 984
BfuAI	ACCTGC	2 cut(s)	200, 698
BglI	GCCNNNNNGGC	2 cut(s)	84, 595
BisI	GCNGC	1 cut(s)	1491
BlsI	GCNGC	1 cut(s)	1492
Bme1390I	CCNGG	4 cut(s)	6, 300, 696, 999
Bme18I	GGWCC	4 cut(s)	212, 449, 992, 1329
BmgT120I	GGNCC	6 cut(s)	212, 449, 587, 992, 1329, 1477
BmiI	GGNNCC	3 cut(s)	297, 1321, 1569
BmrFI	CCNGG	4 cut(s)	6, 300, 696, 999
BmrI	ACTGGG	1 cut(s)	1090
BmsI	GCATC	5 cut(s)	408, 675, 706, 768, 1149
BmuI	ACTGGG	1 cut(s)	1090
BpiI	GAAGAC	2 cut(s)	801, 1586
BpmI	CTGGAG	1 cut(s)	1164
Bpu10I	CCTNAGC	1 cut(s)	1344
Bsa29I	ATCGAT	2 cut(s)	1157, 1604
BsaBI	GATNNNNATC	2 cut(s)	97, 1233
BsaI	GGTCTC	2 cut(s)	405, 1680
BsaJI	CCNNGG	5 cut(s)	215, 231, 625, 1325, 1431
BsaWI	WCCGGW	1 cut(s)	1689
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 199, 200, 443, 676, 1004, 1346
Bse118I	RCCGGY	1 cut(s)	1689
Bse1I	ACTGG	2 cut(s)	283, 1096
Bse21I	CCTNAGG	1 cut(s)	591
Bse8I	GATNNNNATC	2 cut(s)	97, 1233
BseBI	CCWGG	4 cut(s)	6, 300, 696, 999
BseCI	ATCGAT	2 cut(s)	1157, 1604
BseDI	CCNNGG	5 cut(s)	215, 231, 625, 1325, 1431
BseGI	GGATG	3 cut(s)	666, 1227, 1241
BseJI	GATNNNNATC	2 cut(s)	97, 1233
BseLI	CCNNNNNNNGG	7 cut(s)	53, 199, 200, 443, 676, 1004, 1346
BseMII	CTCAG	3 cut(s)	140, 605, 800
BseNI	ACTGG	2 cut(s)	283, 1096
BseRI	GAGGAG	2 cut(s)	189, 280
BseXI	GCAGC	1 cut(s)	1477
BsgI	GTGCAG	2 cut(s)	1091, 1383
BshFI	GGCC	5 cut(s)	589, 624, 850, 1015, 1478
BshNI	GGYRCC	1 cut(s)	295
BshTI	ACCGGT	1 cut(s)	1689
BshVI	ATCGAT	2 cut(s)	1157, 1604
BsiHKAI	GWGCWC	1 cut(s)	491
BsiSI	CCGG	1 cut(s)	1690
BslFI	GGGAC	2 cut(s)	175, 816
BslI	CCNNNNNNNGG	7 cut(s)	53, 199, 200, 443, 676, 1004, 1346
BsmAI	GTCTC	4 cut(s)	129, 405, 898, 1680
BsmFI	GGGAC	2 cut(s)	175, 816
BsnI	GGCC	5 cut(s)	589, 624, 850, 1015, 1478
Bso31I	GGTCTC	2 cut(s)	405, 1680
Bsp1286I	GDGCHC	1 cut(s)	491
Bsp1407I	TGTACA	1 cut(s)	355
Bsp143I	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
Bsp19I	CCATGG	2 cut(s)	231, 1325
BspACI	CCGC	5 cut(s)	87, 404, 581, 1394, 1537
BspANI	GGCC	5 cut(s)	589, 624, 850, 1015, 1478
BspCNI	CTCAG	3 cut(s)	139, 604, 799
BspDI	ATCGAT	2 cut(s)	1157, 1604
BspLI	GGNNCC	3 cut(s)	297, 1321, 1569
BspMI	ACCTGC	2 cut(s)	200, 698
BspPI	GGATC	8 cut(s)	346, 737, 927, 1149, 1223, 1346, 1609, 1615
BspT107I	GGYRCC	1 cut(s)	295
BspTNI	GGTCTC	2 cut(s)	405, 1680
BsrFI	RCCGGY	1 cut(s)	1689
BsrGI	TGTACA	1 cut(s)	355
BsrI	ACTGG	2 cut(s)	283, 1096
BssAI	RCCGGY	1 cut(s)	1689
BssECI	CCNNGG	5 cut(s)	215, 231, 625, 1325, 1431
BssMI	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
BssT1I	CCWWGG	3 cut(s)	231, 625, 1325
Bst2UI	CCWGG	4 cut(s)	6, 300, 696, 999
Bst4CI	ACNGT	4 cut(s)	355, 527, 807, 1712
Bst6I	CTCTTC	1 cut(s)	1678
BstAUI	TGTACA	1 cut(s)	355
BstC8I	GCNNGC	5 cut(s)	1106, 1123, 1341, 1398, 1463
BstDEI	CTNAG	4 cut(s)	126, 591, 786, 1344
BstDSI	CCRYGG	3 cut(s)	231, 1325, 1431
BstF5I	GGATG	3 cut(s)	666, 1227, 1241
BstHHI	GCGC	1 cut(s)	1171
BstKTI	GATC	9 cut(s)	341, 732, 922, 1144, 1231, 1237, 1354, 1604, 1623
BstMAI	GTCTC	4 cut(s)	129, 405, 898, 1680
BstMBI	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
BstMWI	GCNNNNNNNGC	7 cut(s)	84, 595, 787, 856, 1069, 1168, 1175
BstNI	CCWGG	4 cut(s)	6, 300, 696, 999
BstNSI	RCATGY	2 cut(s)	869, 1465
BstSCI	CCNGG	4 cut(s)	4, 298, 694, 997
BstV1I	GCAGC	1 cut(s)	1477
BstV2I	GAAGAC	2 cut(s)	801, 1586
BstX2I	RGATCY	2 cut(s)	338, 919
BstXI	CCANNNNNNTGG	1 cut(s)	935
BstYI	RGATCY	2 cut(s)	338, 919
Bsu15I	ATCGAT	2 cut(s)	1157, 1604
Bsu36I	CCTNAGG	1 cut(s)	591
BsuRI	GGCC	5 cut(s)	589, 624, 850, 1015, 1478
BsuTUI	ATCGAT	2 cut(s)	1157, 1604
BtgI	CCRYGG	3 cut(s)	231, 1325, 1431
BtsCI	GGATG	3 cut(s)	666, 1227, 1241
BtsI	GCAGTG	1 cut(s)	1611
BtsIMutI	CAGTG	2 cut(s)	276, 1611
BveI	ACCTGC	2 cut(s)	200, 698
Cac8I	GCNNGC	5 cut(s)	1106, 1123, 1341, 1398, 1463
CaiI	CAGNNNCTG	1 cut(s)	1040
CfoI	GCGC	1 cut(s)	1171
Cfr10I	RCCGGY	1 cut(s)	1689
Cfr13I	GGNCC	6 cut(s)	212, 449, 587, 992, 1329, 1477
ClaI	ATCGAT	2 cut(s)	1157, 1604
Csp6I	GTAC	3 cut(s)	9, 356, 1556
CspAI	ACCGGT	1 cut(s)	1689
CviQI	GTAC	3 cut(s)	9, 356, 1556
DdeI	CTNAG	4 cut(s)	126, 591, 786, 1344
DpnI	GATC	9 cut(s)	340, 731, 921, 1143, 1230, 1236, 1353, 1603, 1622
DpnII	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
DraI	TTTAAA	1 cut(s)	703
Eam1104I	CTCTTC	1 cut(s)	1678
EarI	CTCTTC	1 cut(s)	1678
EciI	GGCGGA	1 cut(s)	102
Ecl136II	GAGCTC	1 cut(s)	489
Eco130I	CCWWGG	3 cut(s)	231, 625, 1325
Eco147I	AGGCCT	2 cut(s)	624, 1015
Eco24I	GRGCYC	1 cut(s)	491
Eco31I	GGTCTC	2 cut(s)	405, 1680
Eco47I	GGWCC	4 cut(s)	212, 449, 992, 1329
Eco53kI	GAGCTC	1 cut(s)	489
Eco57I	CTGAAG	1 cut(s)	795
Eco81I	CCTNAGG	1 cut(s)	591
EcoICRI	GAGCTC	1 cut(s)	489
EcoO109I	RGGNCCY	3 cut(s)	212, 587, 992
EcoRI	GAATTC	3 cut(s)	514, 1021, 1288
EcoRII	CCWGG	4 cut(s)	4, 298, 694, 997
EcoT14I	CCWWGG	3 cut(s)	231, 625, 1325
EcoT22I	ATGCAT	1 cut(s)	936
EcoT38I	GRGCYC	1 cut(s)	491
ErhI	CCWWGG	3 cut(s)	231, 625, 1325
FaqI	GGGAC	2 cut(s)	175, 816
FauI	CCCGC	1 cut(s)	574
FauNDI	CATATG	2 cut(s)	930, 1054
Fnu4HI	GCNGC	1 cut(s)	1491
FokI	GGATG	3 cut(s)	653, 1228, 1234
FriOI	GRGCYC	1 cut(s)	491
Fsp4HI	GCNGC	1 cut(s)	1491
FspBI	CTAG	3 cut(s)	422, 573, 984
GlaI	GCGC	1 cut(s)	1170
GluI	GCNGC	1 cut(s)	1491
GsuI	CTGGAG	1 cut(s)	1164
HaeIII	GGCC	5 cut(s)	589, 624, 850, 1015, 1478
HapII	CCGG	1 cut(s)	1690
HhaI	GCGC	1 cut(s)	1171
Hin6I	GCGC	1 cut(s)	1169
HinP1I	GCGC	1 cut(s)	1169
HinfI	GANTC	3 cut(s)	57, 93, 162
HpaII	CCGG	1 cut(s)	1690
HphI	GGTGA	2 cut(s)	725, 1361
Hpy166II	GTNNAC	3 cut(s)	452, 608, 910
Hpy188I	TCNGA	6 cut(s)	219, 318, 943, 1020, 1333, 1645
Hpy188III	TCNNGA	8 cut(s)	21, 159, 342, 813, 917, 1181, 1232, 1355
Hpy8I	GTNNAC	3 cut(s)	452, 608, 910
Hpy99I	CGWCG	1 cut(s)	1337
HpyAV	CCTTC	4 cut(s)	57, 119, 564, 1313
HpyCH4III	ACNGT	4 cut(s)	355, 527, 807, 1712
HpyCH4IV	ACGT	2 cut(s)	1439, 1589
HpyF10VI	GCNNNNNNNGC	7 cut(s)	84, 595, 787, 856, 1069, 1168, 1175
HpyF3I	CTNAG	4 cut(s)	126, 591, 786, 1344
HpySE526I	ACGT	2 cut(s)	1439, 1589
HspAI	GCGC	1 cut(s)	1169
Kzo9I	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
LmnI	GCTCC	4 cut(s)	149, 874, 1183, 1567
Lsp1109I	GCAGC	1 cut(s)	1477
LweI	GCATC	5 cut(s)	408, 675, 706, 768, 1149
MaeI	CTAG	3 cut(s)	422, 573, 984
MaeII	ACGT	2 cut(s)	1439, 1589
MaeIII	GTNAC	3 cut(s)	163, 1376, 1694
MalI	GATC	9 cut(s)	340, 731, 921, 1143, 1230, 1236, 1353, 1603, 1622
MboI	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
MboII	GAAGA	5 cut(s)	801, 831, 914, 1591, 1695
MflI	RGATCY	2 cut(s)	338, 919
MhlI	GDGCHC	1 cut(s)	491
MlyI	GAGTC	2 cut(s)	66, 171
MmeI	TCCRAC	3 cut(s)	85, 1356, 1658
Mph1103I	ATGCAT	1 cut(s)	936
MroXI	GAANNNNTTC	2 cut(s)	835, 1302
MslI	CAYNNNNRTG	3 cut(s)	762, 933, 1220
MspA1I	CMGCKG	2 cut(s)	71, 1121
MspI	CCGG	1 cut(s)	1690
MspR9I	CCNGG	4 cut(s)	6, 300, 696, 999
MvaI	CCWGG	4 cut(s)	6, 300, 696, 999
MwoI	GCNNNNNNNGC	7 cut(s)	84, 595, 787, 856, 1069, 1168, 1175
NcoI	CCATGG	2 cut(s)	231, 1325
NdeI	CATATG	2 cut(s)	930, 1054
NdeII	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
NlaIV	GGNNCC	3 cut(s)	297, 1321, 1569
NmuCI	GTSAC	1 cut(s)	163
NsiI	ATGCAT	1 cut(s)	936
NspI	RCATGY	2 cut(s)	869, 1465
PaeI	GCATGC	1 cut(s)	1465
PaqCI	CACCTGC	1 cut(s)	698
PceI	AGGCCT	2 cut(s)	624, 1015
PdmI	GAANNNNTTC	2 cut(s)	835, 1302
PfeI	GAWTC	1 cut(s)	93
PinAI	ACCGGT	1 cut(s)	1689
PkrI	GCNGC	1 cut(s)	1492
PleI	GAGTC	2 cut(s)	65, 170
PpsI	GAGTC	2 cut(s)	65, 170
PpuMI	RGGWCCY	2 cut(s)	212, 992
Psp124BI	GAGCTC	1 cut(s)	491
Psp1406I	AACGTT	1 cut(s)	1439
Psp5II	RGGWCCY	2 cut(s)	212, 992
Psp6I	CCWGG	4 cut(s)	4, 298, 694, 997
PspGI	CCWGG	4 cut(s)	4, 298, 694, 997
PspN4I	GGNNCC	3 cut(s)	297, 1321, 1569
PspPI	GGNCC	6 cut(s)	212, 449, 587, 992, 1329, 1477
PspPPI	RGGWCCY	2 cut(s)	212, 992
PstNI	CAGNNNCTG	1 cut(s)	1040
PsuI	RGATCY	2 cut(s)	338, 919
PvuII	CAGCTG	2 cut(s)	71, 1121
RsaI	GTAC	3 cut(s)	10, 357, 1557
RsaNI	GTAC	3 cut(s)	9, 356, 1556
RseI	CAYNNNNRTG	3 cut(s)	762, 933, 1220
SacI	GAGCTC	1 cut(s)	491
SatI	GCNGC	1 cut(s)	1491
Sau3AI	GATC	9 cut(s)	338, 729, 919, 1141, 1228, 1234, 1351, 1601, 1620
Sau96I	GGNCC	6 cut(s)	212, 449, 587, 992, 1329, 1477
SchI	GAGTC	2 cut(s)	66, 171
ScrFI	CCNGG	4 cut(s)	6, 300, 696, 999
SduI	GDGCHC	1 cut(s)	491
SfaNI	GCATC	5 cut(s)	408, 675, 706, 768, 1149
SinI	GGWCC	4 cut(s)	212, 449, 992, 1329
SmiMI	CAYNNNNRTG	3 cut(s)	762, 933, 1220
SpeI	ACTAGT	1 cut(s)	983
SphI	GCATGC	1 cut(s)	1465
SseBI	AGGCCT	2 cut(s)	624, 1015
SsiI	CCGC	5 cut(s)	87, 404, 581, 1394, 1537
SspI	AATATT	1 cut(s)	1720
SspMI	CTAG	3 cut(s)	422, 573, 984
SstI	GAGCTC	1 cut(s)	491
StuI	AGGCCT	2 cut(s)	624, 1015
StyD4I	CCNGG	4 cut(s)	4, 298, 694, 997
StyI	CCWWGG	3 cut(s)	231, 625, 1325
TaaI	ACNGT	4 cut(s)	355, 527, 807, 1712
TaiI	ACGT	2 cut(s)	1442, 1592
TaqI	TCGA	5 cut(s)	416, 491, 773, 1157, 1604
TatI	WGTACW	1 cut(s)	355
TfiI	GAWTC	1 cut(s)	93
TscAI	CASTG	2 cut(s)	283, 1618
TseFI	GTSAC	1 cut(s)	163
TseI	GCWGC	1 cut(s)	1490
Tsp45I	GTSAC	1 cut(s)	163
TspDTI	ATGAA	6 cut(s)	518, 546, 1041, 1166, 1512, 1537
TspGWI	ACGGA	1 cut(s)	1420
TspRI	CASTG	2 cut(s)	283, 1618
VpaK11BI	GGWCC	4 cut(s)	212, 449, 992, 1329
XapI	RAATTY	5 cut(s)	319, 427, 514, 1021, 1288
XceI	RCATGY	2 cut(s)	869, 1465
XcmI	CCANNNNNNNNNTGG	3 cut(s)	68, 238, 1118
XmnI	GAANNNNTTC	2 cut(s)	835, 1302
XspI	CTAG	3 cut(s)	422, 573, 984
Zsp2I	ATGCAT	1 cut(s)	936

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.