pycom11g10420

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012123
Physical Location & Seq
Forward (+)
8847765 .. 8848622
858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGCTGGTCTGCTCAAAATCCCAACAGCAGCAGCAAGACAGGAAGCCCAGGCCTCAGCCAGAACAAGCCCTAAAGTGTCCAAGATGTGACTCCACAAACACGAAGTTCTGTTACTACAACAACTACAGCCTCACTCAGCCCAGGTACTTCTGCAAGTCCTGCAGAAGGTACTGGACCAAAGGTGGCACCCTCAGGAACGTTCCTGTGGGTGGAGGCTGCAGGAAGAACAAGAGATCATCGTCAACGAAACGAGCTCAGGACCAACCGCTCACACCCAATTCCAACCCACTCCCAGCCCTAGGTTATGACTCAAATGACCTCACCCTTGCATTTGCTAGGCTCCAAAGGCAACAGTCATGTGGTGGGCAGTTAGGATTTGATGACCATGACCACATTACTCTCTTGGGAAATCCCACAAATGCCCCTGATCATAACATTCTTGGAAACCCTACTTCAGCTCCTGGGTTTCTTGATGCACTGAGGACTGGGTTTCTTGAGACCCACAATGGTAATTTCCAGAACTTGTATAACTATGGGTATGGGAGTGGGAATGGGAATTTGGGTGAGGTGGAAGATTGTGGTGGCGGAATGGGAATCCACCACCCATTTGAAGAAGCCACCACCAACTCAACCACCACAGCAGTGACAGTTACAACAATGAAGCAAGAACTGTTCAATGGCCAAAGTGAGAGCAGCAACAGAGTTCTGTGGGGGTTTCCATGGCAGATGAACGGTGGAGATCATCATTCAAACATGATGGGGAATGGTAATCACGAGTATGATTCAGGAAGAGAGAGCTGGAATGGGATTGCTTCATCTTGGCAGCATGGACTTCTGCACACTACTCCCCTCGTGTAA

Protein Analysis

286

Amino Acids

31.55

Weight (kDa)

8.07

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 22 - 78 3.4e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28510 AT3G45610 AT5G60200
fragaria_vesca FvH4_3g33660 FvH4_3g33660
malus_domestica MD03G1110000.v1.1 MD11G1123400.v1.1
prunus_persica Prupe.6G092600_v2.0.a1
pyrus_communis pycom11g10420
rosa_chinensis RchiOBHm_Chr5g0061151
rosa_laevigata RLG00000035436
rosa_multiflora Rmu_sc0001366.1_g000022
rosa_roxburghii Rroxscaffold_1G00019530
rosa_rugosa Rorug05G0338400
rosa_samantha Rh5AG399100 Rh5BG412300 Rh5CG437100 Rh5DG426600
rosa_wichuraiana Rw5G037660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 268
AciI CCGC 2 cut(s) 266, 585
AclI AACGTT 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 2 cut(s) 146, 170
AfiI CCNNNNNNNGG 3 cut(s) 165, 209, 299
AgsI TTSAA 3 cut(s) 611, 676, 750
AjnI CCWGG 3 cut(s) 47, 140, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AleI CACNNNNGTG 1 cut(s) 641
AluBI AGCT 3 cut(s) 254, 458, 798
AluI AGCT 3 cut(s) 254, 458, 798
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 2 cut(s) 50, 679
ApeKI GCWGC 5 cut(s) 28, 31, 216, 693, 823
ApoI RAATTY 1 cut(s) 556
AspA2I CCTAGG 1 cut(s) 298
AspS9I GGNCC 2 cut(s) 174, 259
AsuHPI GGTGA 2 cut(s) 313, 575
AvaII GGWCC 2 cut(s) 174, 259
AvrII CCTAGG 1 cut(s) 298
AxyI CCTNAGG 1 cut(s) 191
BalI TGGCCA 1 cut(s) 681
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BauI CACGAG 2 cut(s) 773, 851
Bbv12I GWGCWC 1 cut(s) 256
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 5 cut(s) 40, 43, 203, 705, 835
BccI CCATC 1 cut(s) 751
BciT130I CCWGG 3 cut(s) 49, 142, 462
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 2 cut(s) 299, 336
BfmI CTRYAG 3 cut(s) 124, 160, 217
BisI GCNGC 5 cut(s) 29, 32, 217, 694, 824
BlnI CCTAGG 1 cut(s) 298
BlsI GCNGC 5 cut(s) 30, 33, 218, 695, 825
Bme1390I CCNGG 3 cut(s) 49, 142, 462
Bme18I GGWCC 2 cut(s) 174, 259
BmgT120I GGNCC 2 cut(s) 174, 259
BmiI GGNNCC 2 cut(s) 187, 341
BmrFI CCNGG 3 cut(s) 49, 142, 462
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 495
Bpu10I CCTNAGC 2 cut(s) 54, 255
BpuEI CTTGAG 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 5 cut(s) 47, 140, 298, 461, 719
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 209, 299
Bse1I ACTGG 2 cut(s) 176, 490
Bse21I CCTNAGG 1 cut(s) 191
BseBI CCWGG 3 cut(s) 49, 142, 462
BseDI CCNNGG 5 cut(s) 47, 140, 298, 461, 719
BseLI CCNNNNNNNGG 3 cut(s) 165, 209, 299
BseMII CTCAG 5 cut(s) 68, 149, 205, 269, 470
BseNI ACTGG 2 cut(s) 176, 490
BseXI GCAGC 5 cut(s) 40, 43, 203, 705, 835
BseYI CCCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 821
BshFI GGCC 2 cut(s) 52, 681
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 165, 209, 299
BsmAI GTCTC 1 cut(s) 491
BsnI GGCC 2 cut(s) 52, 681
Bso31I GGTCTC 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 3 cut(s) 233, 427, 739
Bsp19I CCATGG 1 cut(s) 719
BspACI CCGC 2 cut(s) 266, 585
BspANI GGCC 2 cut(s) 52, 681
BspCNI CTCAG 5 cut(s) 67, 148, 204, 268, 471
BspLI GGNNCC 2 cut(s) 187, 341
BspMAI CTGCAG 2 cut(s) 164, 221
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 268
BsrI ACTGG 2 cut(s) 176, 490
BssECI CCNNGG 5 cut(s) 47, 140, 298, 461, 719
BssMI GATC 3 cut(s) 233, 427, 739
BssSI CACGAG 2 cut(s) 773, 851
BssT1I CCWWGG 2 cut(s) 298, 719
Bst2BI CACGAG 2 cut(s) 773, 851
Bst2UI CCWGG 3 cut(s) 49, 142, 462
Bst4CI ACNGT 4 cut(s) 354, 649, 672, 734
Bst6I CTCTTC 1 cut(s) 784
BstAPI GCANNNNNTGC 1 cut(s) 159
BstDEI CTNAG 5 cut(s) 54, 135, 191, 255, 479
BstDSI CCRYGG 1 cut(s) 719
BstENI CCTNNNNNAGG 1 cut(s) 163
BstKTI GATC 3 cut(s) 236, 430, 742
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 3 cut(s) 233, 427, 739
BstMWI GCNNNNNNNGC 2 cut(s) 159, 346
BstNI CCWGG 3 cut(s) 49, 142, 462
BstSCI CCNGG 3 cut(s) 47, 140, 460
BstSFI CTRYAG 3 cut(s) 124, 160, 217
BstV1I GCAGC 5 cut(s) 40, 43, 203, 705, 835
Bsu36I CCTNAGG 1 cut(s) 191
BsuRI GGCC 2 cut(s) 52, 681
BtgI CCRYGG 1 cut(s) 719
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 476, 648
CaiI CAGNNNCTG 1 cut(s) 461
Cfr13I GGNCC 2 cut(s) 174, 259
Csp6I GTAC 2 cut(s) 145, 169
CviAII CATG 5 cut(s) 357, 386, 720, 754, 827
CviQI GTAC 2 cut(s) 145, 169
DdeI CTNAG 5 cut(s) 54, 135, 191, 255, 479
DpnI GATC 3 cut(s) 235, 429, 741
DpnII GATC 3 cut(s) 233, 427, 739
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 784
EarI CTCTTC 1 cut(s) 784
EciI GGCGGA 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 298, 719
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 174, 259
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 438
Eco81I CCTNAGG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 254
EcoNI CCTNNNNNAGG 1 cut(s) 163
EcoRII CCWGG 3 cut(s) 47, 140, 460
EcoT14I CCWWGG 2 cut(s) 298, 719
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 2 cut(s) 298, 719
FaeI CATG 5 cut(s) 360, 389, 723, 757, 830
FatI CATG 5 cut(s) 356, 385, 719, 753, 826
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 5 cut(s) 29, 32, 217, 694, 824
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 5 cut(s) 29, 32, 217, 694, 824
FspBI CTAG 2 cut(s) 299, 336
GluI GCNGC 5 cut(s) 29, 32, 217, 694, 824
GsaI CCCAGC 1 cut(s) 296
HaeIII GGCC 2 cut(s) 52, 681
Hin1II CATG 5 cut(s) 360, 389, 723, 757, 830
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 4 cut(s) 89, 308, 594, 782
HphI GGTGA 2 cut(s) 313, 575
Hpy166II GTNNAC 1 cut(s) 243
Hpy188III TCNNGA 7 cut(s) 193, 257, 470, 494, 517, 773, 786
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 4 cut(s) 354, 649, 672, 734
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 6 cut(s) 153, 162, 219, 329, 476, 838
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 346
HpyF3I CTNAG 5 cut(s) 54, 135, 191, 255, 479
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 5 cut(s) 360, 389, 723, 757, 830
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 3 cut(s) 233, 427, 739
LmnI GCTCC 2 cut(s) 345, 463
Lsp1109I GCAGC 5 cut(s) 40, 43, 203, 705, 835
LweI GCATC 1 cut(s) 463
MaeI CTAG 2 cut(s) 299, 336
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 4 cut(s) 86, 110, 643, 649
MalI GATC 3 cut(s) 235, 429, 741
MbiI CCGCTC 1 cut(s) 268
MboI GATC 3 cut(s) 233, 427, 739
MboII GAAGA 4 cut(s) 235, 584, 623, 801
MhlI GDGCHC 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 3 cut(s) 277, 511, 556
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 2 cut(s) 83, 302
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 7 cut(s) 63, 140, 200, 206, 329, 474, 559
Mox20I TGGCCA 1 cut(s) 681
MscI TGGCCA 1 cut(s) 681
MslI CAYNNNNRTG 2 cut(s) 641, 777
Msp20I TGGCCA 1 cut(s) 681
MspR9I CCNGG 3 cut(s) 49, 142, 462
MvaI CCWGG 3 cut(s) 49, 142, 462
MwoI GCNNNNNNNGC 2 cut(s) 159, 346
NcoI CCATGG 1 cut(s) 719
NdeII GATC 3 cut(s) 233, 427, 739
NlaIII CATG 5 cut(s) 360, 389, 723, 757, 830
NlaIV GGNNCC 2 cut(s) 187, 341
NmuCI GTSAC 2 cut(s) 86, 643
OliI CACNNNNGTG 1 cut(s) 641
PceI AGGCCT 1 cut(s) 52
PfeI GAWTC 2 cut(s) 594, 782
PkrI GCNGC 5 cut(s) 30, 33, 218, 695, 825
PleI GAGTC 2 cut(s) 83, 302
PpsI GAGTC 2 cut(s) 83, 302
Psp124BI GAGCTC 1 cut(s) 256
Psp1406I AACGTT 1 cut(s) 198
Psp6I CCWGG 3 cut(s) 47, 140, 460
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 3 cut(s) 47, 140, 460
PspN4I GGNNCC 2 cut(s) 187, 341
PspPI GGNCC 2 cut(s) 174, 259
PstI CTGCAG 2 cut(s) 164, 221
PstNI CAGNNNCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 146, 170
RsaNI GTAC 2 cut(s) 145, 169
RseI CAYNNNNRTG 2 cut(s) 641, 777
SacI GAGCTC 1 cut(s) 256
SatI GCNGC 5 cut(s) 29, 32, 217, 694, 824
Sau3AI GATC 3 cut(s) 233, 427, 739
Sau96I GGNCC 2 cut(s) 174, 259
SchI GAGTC 2 cut(s) 83, 302
ScrFI CCNGG 3 cut(s) 49, 142, 462
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 3 cut(s) 124, 160, 217
SinI GGWCC 2 cut(s) 174, 259
SmiMI CAYNNNNRTG 2 cut(s) 641, 777
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 3 cut(s) 277, 511, 556
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 2 cut(s) 266, 585
SspMI CTAG 2 cut(s) 299, 336
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 3 cut(s) 47, 140, 460
StyI CCWWGG 2 cut(s) 298, 719
TaaI ACNGT 4 cut(s) 354, 649, 672, 734
TaiI ACGT 1 cut(s) 201
TasI AATT 3 cut(s) 277, 511, 556
TfiI GAWTC 2 cut(s) 594, 782
TscAI CASTG 2 cut(s) 483, 648
TseFI GTSAC 2 cut(s) 86, 643
TseI GCWGC 5 cut(s) 28, 31, 216, 693, 823
Tsp45I GTSAC 2 cut(s) 86, 643
TspDTI ATGAA 3 cut(s) 674, 743, 804
TspRI CASTG 2 cut(s) 483, 648
VpaK11BI GGWCC 2 cut(s) 174, 259
XagI CCTNNNNNAGG 1 cut(s) 163
XapI RAATTY 1 cut(s) 556
XmaJI CCTAGG 1 cut(s) 298
XspI CTAG 2 cut(s) 299, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.