RchiOBHm_Chr5g0061151

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012123
Physical Location & Seq
Reverse (-)
66499802 .. 66502035
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33754

Sequence Viewer

Length: 948 bp
ATGGATCCTTCAAGCGGACAACAAAACCAGGATATGCAAGCTCATAATTCCCTAGACATGTTGGTCAGCTCAAAATCCCAGCAGCAAACAGACCAGAGAAAACCAAGGCCACAACCAGAACAAGCCCTCAAGTGTCCAAGATGTGACTCCACCAACACCAAGTTCTGCTACTACAACAACTACAGCCTCACCCAGCCCAGGTACTTCTGCAAGTCCTGCAGGAGGTACTGGACCAAAGGCGGCACACTGCGGAACGTTCCGGTGGGCGGTGGCTGCCGCAAGAACAAGAGATCCTCATCGAAGCGATCGTCGCAAGACCCTCAACAACCACTCACTCCCAACTCCAACCCGCTCCTCCCATCGCTCTCCTACGACTCCAATGACCTCAGTCTTGCCTTTGCTAGGCTCCAGAGGTCATGTGGGCAGTTGGGTTATGATGATCACCTATCCATGTTAGGAAACCCAACCACCAATTCCCACAGCGACATTTTGTCGAACGGTTCGTCTGCATCAGCATCGCCAGGGTTTCTGGATGCTCTCCGGACTGGGTTTCTCGAAACCCACAACAGCAACAGCTTCCAGAACAACTTGTATTATGGGTTTGGATCAAGTGGGGGGAACGCCATGGTGGGTGAGGCGGAAATGGACAATGGGGTTTGCGGAGAAATGGGGCTGCACCCTTATGAGGAATTGAGCAATGTAGCAACCACAACAGCTGTGACGGTCACAACAATGAAGCAAGAGCTGAGCAGAGATATTGAGACCAACAACAACAGAGTCTTGTGGGGGTTTCCATGGCAGATGAACAATGGAGATCATCACAACTTGAACATGGGTGGTGGTGGTGGTGGTGGTGGTGCTCATCATGATCAGTATGATAACTCGGGAAGAGAGAGCTGGAATGGACTTGCAAATCCATCTTGGCATGGACTTCTTCCCCTAGTTTAA

Protein Analysis

315

Amino Acids

34.54

Weight (kDa)

6.78

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 41 - 97 4e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28510 AT3G45610 AT5G60200
fragaria_vesca FvH4_3g33660 FvH4_3g33660
malus_domestica MD03G1110000.v1.1 MD11G1123400.v1.1
prunus_persica Prupe.6G092600_v2.0.a1
pyrus_communis pycom11g10420
rosa_chinensis RchiOBHm_Chr5g0061151
rosa_laevigata RLG00000035436
rosa_multiflora Rmu_sc0001366.1_g000022
rosa_roxburghii Rroxscaffold_1G00019530
rosa_rugosa Rorug05G0338400
rosa_samantha Rh5AG399100 Rh5BG412300 Rh5CG437100 Rh5DG426600
rosa_wichuraiana Rw5G037660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 352
AccIII TCCGGA 1 cut(s) 540
AciI CCGC 8 cut(s) 15, 240, 250, 267, 277, 350, 638, 660
AclI AACGTT 1 cut(s) 255
AclWI GGATC 3 cut(s) 12, 285, 613
AfaI GTAC 2 cut(s) 203, 227
AfiI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 2 cut(s) 12, 829
AhdI GACNNNNNGTC 1 cut(s) 490
AjnI CCWGG 3 cut(s) 27, 197, 520
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 457, 489
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 6 cut(s) 41, 69, 576, 716, 745, 897
AluI AGCT 6 cut(s) 41, 69, 576, 716, 745, 897
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 3 cut(s) 12, 285, 613
Ama87I CYCGRG 1 cut(s) 883
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 3 cut(s) 82, 273, 673
ArsI GACNNNNNNTTYG 4 cut(s) 293, 325, 488, 520
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 3 cut(s) 181, 434, 644
AvaI CYCGRG 1 cut(s) 883
AvaII GGWCC 1 cut(s) 231
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 862
BbvI GCAGC 3 cut(s) 94, 260, 660
BccI CCATC 2 cut(s) 367, 925
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 3 cut(s) 29, 199, 522
BclI TGATCA 2 cut(s) 439, 868
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 3 cut(s) 53, 402, 941
BfmI CTRYAG 2 cut(s) 181, 217
BisI GCNGC 5 cut(s) 83, 241, 274, 277, 674
BlpI GCTNAGC 1 cut(s) 746
BlsI GCNGC 5 cut(s) 84, 242, 275, 278, 675
Bme1390I CCNGG 3 cut(s) 29, 199, 522
Bme18I GGWCC 1 cut(s) 231
BmeRI GACNNNNNGTC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 883
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 6, 407
BmrFI CCNGG 3 cut(s) 29, 199, 522
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 3 cut(s) 518, 523, 524
BmuI ACTGGG 1 cut(s) 555
BoxI GACNNNNGTC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 746
BpuEI CTTGAG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BsaWI WCCGGW 2 cut(s) 259, 540
Bsc4I CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
Bse1I ACTGG 2 cut(s) 233, 550
Bse3DI GCAATG 1 cut(s) 703
Bse8I GATNNNNATC 1 cut(s) 295
BseAI TCCGGA 1 cut(s) 540
BseBI CCWGG 3 cut(s) 29, 199, 522
BseDI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BseGI GGATG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 295
BseLI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 2 cut(s) 400, 737
BseNI ACTGG 2 cut(s) 233, 550
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 94, 260, 660
BseYI CCCAGC 2 cut(s) 78, 192
BsgI GTGCAG 1 cut(s) 659
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 862
BsiHKCI CYCGRG 1 cut(s) 883
BsiSI CCGG 2 cut(s) 260, 541
BslI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
BsmAI GTCTC 1 cut(s) 755
BsnI GGCC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 862
Bsp13I TCCGGA 1 cut(s) 540
Bsp143I GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
Bsp1720I GCTNAGC 1 cut(s) 746
Bsp19I CCATGG 2 cut(s) 624, 794
BspACI CCGC 8 cut(s) 15, 240, 250, 267, 277, 350, 638, 660
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 2 cut(s) 399, 738
BspEI TCCGGA 1 cut(s) 540
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 2 cut(s) 6, 407
BspMAI CTGCAG 1 cut(s) 221
BspPI GGATC 3 cut(s) 12, 285, 613
BspTNI GGTCTC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 703
BsrI ACTGG 2 cut(s) 233, 550
BssECI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BssMI GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
BssT1I CCWWGG 3 cut(s) 104, 624, 794
Bst2UI CCWGG 3 cut(s) 29, 199, 522
Bst4CI ACNGT 2 cut(s) 500, 724
Bst6I CTCTTC 1 cut(s) 883
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 386, 746
BstDSI CCRYGG 2 cut(s) 624, 794
BstENI CCTNNNNNAGG 2 cut(s) 220, 400
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 7 cut(s) 7, 293, 308, 442, 608, 817, 871
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 216, 273, 310
BstNI CCWGG 3 cut(s) 29, 199, 522
BstNSI RCATGY 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 3 cut(s) 27, 197, 520
BstSFI CTRYAG 2 cut(s) 181, 217
BstV1I GCAGC 3 cut(s) 94, 260, 660
BstX2I RGATCY 2 cut(s) 4, 290
BstYI RGATCY 2 cut(s) 4, 290
BsuRI GGCC 1 cut(s) 109
BtgI CCRYGG 2 cut(s) 624, 794
BtgZI GCGATG 2 cut(s) 345, 501
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 39
CciI TCATGA 1 cut(s) 865
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 202, 226
CviAII CATG 8 cut(s) 58, 417, 451, 625, 795, 832, 866, 926
CviQI GTAC 2 cut(s) 202, 226
DdeI CTNAG 2 cut(s) 386, 746
DpnI GATC 7 cut(s) 6, 292, 307, 441, 607, 816, 870
DpnII GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
DrdI GACNNNNNNGTC 1 cut(s) 62
DriI GACNNNNNGTC 1 cut(s) 490
DseDI GACNNNNNNGTC 1 cut(s) 62
Eam1104I CTCTTC 1 cut(s) 883
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 883
EciI GGCGGA 1 cut(s) 653
Eco130I CCWWGG 3 cut(s) 104, 624, 794
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 883
EcoNI CCTNNNNNAGG 2 cut(s) 220, 400
EcoRII CCWGG 3 cut(s) 27, 197, 520
EcoT14I CCWWGG 3 cut(s) 104, 624, 794
ErhI CCWWGG 3 cut(s) 104, 624, 794
FaeI CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 869, 929
FatI CATG 8 cut(s) 57, 416, 450, 624, 794, 831, 865, 925
FauI CCCGC 1 cut(s) 357
FbaI TGATCA 2 cut(s) 439, 868
Fnu4HI GCNGC 5 cut(s) 83, 241, 274, 277, 674
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 5 cut(s) 83, 241, 274, 277, 674
FspBI CTAG 3 cut(s) 53, 402, 941
GluI GCNGC 5 cut(s) 83, 241, 274, 277, 674
GsaI CCCAGC 2 cut(s) 82, 196
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 1 cut(s) 109
HapII CCGG 2 cut(s) 260, 541
Hin1II CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 869, 929
HinfI GANTC 3 cut(s) 146, 374, 777
HpaII CCGG 2 cut(s) 260, 541
HphI GGTGA 3 cut(s) 181, 434, 644
Hpy188III TCNNGA 7 cut(s) 409, 530, 541, 554, 580, 866, 885
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 1 cut(s) 18
HpyCH4III ACNGT 2 cut(s) 500, 724
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 6 cut(s) 37, 210, 219, 509, 676, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 273, 310
HpyF3I CTNAG 2 cut(s) 386, 746
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 869, 929
Kpn2I TCCGGA 1 cut(s) 540
Ksp22I TGATCA 2 cut(s) 439, 868
Kzo9I GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
LmnI GCTCC 2 cut(s) 357, 411
Lsp1109I GCAGC 3 cut(s) 94, 260, 660
LweI GCATC 3 cut(s) 518, 523, 524
MaeI CTAG 3 cut(s) 53, 402, 941
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 143, 718, 724
MalI GATC 7 cut(s) 6, 292, 307, 441, 607, 816, 870
MbiI CCGCTC 1 cut(s) 352
MboI GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
MboII GAAGA 2 cut(s) 900, 926
MflI RGATCY 2 cut(s) 4, 290
MhlI GDGCHC 1 cut(s) 862
MluCI AATT 3 cut(s) 46, 472, 689
MlyI GAGTC 3 cut(s) 140, 368, 786
MmeI TCCRAC 1 cut(s) 369
MroI TCCGGA 1 cut(s) 540
MseI TTAA 1 cut(s) 946
MslI CAYNNNNRTG 2 cut(s) 681, 731
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 2 cut(s) 260, 541
MspR9I CCNGG 3 cut(s) 29, 199, 522
MvaI CCWGG 3 cut(s) 29, 199, 522
MwoI GCNNNNNNNGC 3 cut(s) 216, 273, 310
NcoI CCATGG 2 cut(s) 624, 794
NdeII GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
NlaIII CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 869, 929
NlaIV GGNNCC 2 cut(s) 6, 407
NmuCI GTSAC 3 cut(s) 143, 718, 724
NspI RCATGY 1 cut(s) 61
PagI TCATGA 1 cut(s) 865
PciI ACATGT 1 cut(s) 57
PcsI WCGNNNNNNNCGW 2 cut(s) 305, 500
PkrI GCNGC 5 cut(s) 84, 242, 275, 278, 675
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 3 cut(s) 140, 368, 785
PpsI GAGTC 3 cut(s) 140, 368, 785
PscI ACATGT 1 cut(s) 57
PshAI GACNNNNGTC 1 cut(s) 387
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 3 cut(s) 27, 197, 520
PspFI CCCAGC 2 cut(s) 78, 192
PspGI CCWGG 3 cut(s) 27, 197, 520
PspN4I GGNNCC 2 cut(s) 6, 407
PspPI GGNCC 1 cut(s) 231
PstI CTGCAG 1 cut(s) 221
PsuI RGATCY 2 cut(s) 4, 290
PvuI CGATCG 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 2 cut(s) 203, 227
RsaNI GTAC 2 cut(s) 202, 226
RseI CAYNNNNRTG 2 cut(s) 681, 731
SaqAI TTAA 1 cut(s) 946
SatI GCNGC 5 cut(s) 83, 241, 274, 277, 674
Sau3AI GATC 7 cut(s) 4, 290, 305, 439, 605, 814, 868
Sau96I GGNCC 1 cut(s) 231
SbfI CCTGCAGG 1 cut(s) 221
SchI GAGTC 3 cut(s) 140, 368, 786
ScrFI CCNGG 3 cut(s) 29, 199, 522
SdaI CCTGCAGG 1 cut(s) 221
SduI GDGCHC 1 cut(s) 862
SfaNI GCATC 3 cut(s) 518, 523, 524
SfcI CTRYAG 2 cut(s) 181, 217
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 2 cut(s) 681, 731
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse8387I CCTGCAGG 1 cut(s) 221
Sse9I AATT 3 cut(s) 46, 472, 689
SsiI CCGC 8 cut(s) 15, 240, 250, 267, 277, 350, 638, 660
SspMI CTAG 3 cut(s) 53, 402, 941
StyD4I CCNGG 3 cut(s) 27, 197, 520
StyI CCWWGG 3 cut(s) 104, 624, 794
TaaI ACNGT 2 cut(s) 500, 724
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 299, 494, 555
TasI AATT 3 cut(s) 46, 472, 689
TauI GCSGC 2 cut(s) 243, 279
Tru1I TTAA 1 cut(s) 946
Tru9I TTAA 1 cut(s) 946
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 3 cut(s) 143, 718, 724
TseI GCWGC 3 cut(s) 82, 273, 673
Tsp45I GTSAC 3 cut(s) 143, 718, 724
TspDTI ATGAA 2 cut(s) 749, 818
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 231
XagI CCTNNNNNAGG 2 cut(s) 220, 400
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XspI CTAG 3 cut(s) 53, 402, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.