Rroxscaffold_1G00019530

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012123
Physical Location & Seq
Forward (+)
23879782 .. 23881916
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019530.1

Sequence Viewer

Length: 939 bp
ATGGATCCTGCAAGTGGACAACAAAACCAGGAAATGCAAGCTCATAATTCCCTAGACATGTTGGCCAGCTCAAAATCCCAGCAGCAAGCAGACCAGAGAAAACCAAGGCCACAGCCAGAACAAGCCCTCAAGTGCCCAAGATGTGACTCCACCAACACCAAGTTCTGCTACTACAACAACTACAGCCTCACCCAGCCCAGGTACTTCTGCAAGTCCTGCAGGAGGTACTGGACCAAAGGCGGCACACTGCGGAACGTTCCTGTGGGCGGTGGCTGCCGCAAGAACAAGAGATCCTCATCGAAGCGATCGTCGCAAGATCCTCAACAACCACTCACTCCCAACTCCAACCCGCTCCTCCCATCGCTCTCCTACGACTCCAATGACCTCAGTCTTGCCTTTGCTAGGCTCCAGAGGTCATGTGGGCAGTTGGGTTATGATGATCACCTATCCATGTTAGGAAACCCAACCACCAATTCCCACAGCGACATTTTGTCGAACGGCTCATCTGCATCAGCATCGCCAGGGTTTCTGGATGCTCTCCGGACCGGGTTCCTCGAAACCCACAACAGCAACAGCTTCCAGAACAACTTGTATTATGGGTTTGGATCAAGTGGGGGAACCACCATGGTGGGTGAGGCGGAAATGGACAATGGGGTTTGCGGAGAAATGGGGCTGCACCCTTATGAGGAATTGAGCAATGTAGCAACCACAACAGCTGTGACGGTCACAACAATGAAGCAAGAGCTGAGCAGAGATATTGAGACCAACAACAACAGAGTCTTGTGGGGGTTTCCATGGCAGATGAACAATGGAGATCATCACAACTTGAACATGGGTGGTGGTGGTGGTGGTGCTCATCATGAGTATGATAACTCAGGAAGAGAGAGCTGGAATGGACTTGCAAATCCATCTTGGCATGGACTTCTTCCCCTAGTTTAA

Protein Analysis

312

Amino Acids

34.27

Weight (kDa)

6.78

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 41 - 97 3.9e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28510 AT3G45610 AT5G60200
fragaria_vesca FvH4_3g33660 FvH4_3g33660
malus_domestica MD03G1110000.v1.1 MD11G1123400.v1.1
prunus_persica Prupe.6G092600_v2.0.a1
pyrus_communis pycom11g10420
rosa_chinensis RchiOBHm_Chr5g0061151
rosa_laevigata RLG00000035436
rosa_multiflora Rmu_sc0001366.1_g000022
rosa_roxburghii Rroxscaffold_1G00019530
rosa_rugosa Rorug05G0338400
rosa_samantha Rh5AG399100 Rh5BG412300 Rh5CG437100 Rh5DG426600
rosa_wichuraiana Rw5G037660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 352
AccIII TCCGGA 1 cut(s) 540
AciI CCGC 7 cut(s) 240, 250, 267, 277, 350, 638, 660
AclI AACGTT 1 cut(s) 255
AclWI GGATC 4 cut(s) 12, 285, 311, 613
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 2 cut(s) 203, 227
AfiI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 1 cut(s) 829
AhdI GACNNNNNGTC 1 cut(s) 490
AjnI CCWGG 3 cut(s) 27, 197, 520
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 457, 489
AleI CACNNNNGTG 1 cut(s) 626
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 6 cut(s) 41, 69, 576, 716, 745, 888
AluI AGCT 6 cut(s) 41, 69, 576, 716, 745, 888
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 4 cut(s) 12, 285, 311, 613
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 2 cut(s) 63, 107
ApeKI GCWGC 3 cut(s) 82, 273, 673
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspS9I GGNCC 2 cut(s) 231, 543
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 3 cut(s) 181, 434, 644
AvaII GGWCC 2 cut(s) 231, 543
BaeGI GKGCMC 1 cut(s) 137
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 3 cut(s) 94, 260, 660
BccI CCATC 2 cut(s) 367, 916
BceAI ACGGC 1 cut(s) 514
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 3 cut(s) 29, 199, 522
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 3 cut(s) 53, 402, 932
BfmI CTRYAG 2 cut(s) 181, 217
BisI GCNGC 5 cut(s) 83, 241, 274, 277, 674
BlpI GCTNAGC 1 cut(s) 746
BlsI GCNGC 5 cut(s) 84, 242, 275, 278, 675
Bme1390I CCNGG 4 cut(s) 29, 199, 522, 547
Bme18I GGWCC 2 cut(s) 231, 543
BmeRI GACNNNNNGTC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 231, 543
BmiI GGNNCC 4 cut(s) 6, 407, 551, 619
BmrFI CCNGG 4 cut(s) 29, 199, 522, 547
BmsI GCATC 3 cut(s) 518, 523, 524
BoxI GACNNNNGTC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 746
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BsaWI WCCGGW 1 cut(s) 540
Bsc4I CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 703
Bse8I GATNNNNATC 1 cut(s) 295
BseAI TCCGGA 1 cut(s) 540
BseBI CCWGG 3 cut(s) 29, 199, 522
BseDI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BseGI GGATG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 295
BseLI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 3 cut(s) 400, 737, 888
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 3 cut(s) 94, 260, 660
BseYI CCCAGC 2 cut(s) 78, 192
BsgI GTGCAG 1 cut(s) 659
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 65, 109
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 856
BsiSI CCGG 2 cut(s) 541, 546
BslI CCNNNNNNNGG 6 cut(s) 14, 198, 222, 266, 402, 685
BsmAI GTCTC 1 cut(s) 755
BsnI GGCC 2 cut(s) 65, 109
Bso31I GGTCTC 1 cut(s) 755
Bsp1286I GDGCHC 2 cut(s) 137, 856
Bsp13I TCCGGA 1 cut(s) 540
Bsp143I GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
Bsp1720I GCTNAGC 1 cut(s) 746
Bsp19I CCATGG 2 cut(s) 624, 794
BspACI CCGC 7 cut(s) 240, 250, 267, 277, 350, 638, 660
BspANI GGCC 2 cut(s) 65, 109
BspCNI CTCAG 3 cut(s) 399, 738, 887
BspEI TCCGGA 1 cut(s) 540
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 4 cut(s) 6, 407, 551, 619
BspMAI CTGCAG 1 cut(s) 221
BspPI GGATC 4 cut(s) 12, 285, 311, 613
BspTNI GGTCTC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 703
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 104, 197, 521, 624, 794
BssMI GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
BssT1I CCWWGG 3 cut(s) 104, 624, 794
Bst2UI CCWGG 3 cut(s) 29, 199, 522
Bst4CI ACNGT 1 cut(s) 724
Bst6I CTCTTC 1 cut(s) 874
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 39, 67, 87
BstDEI CTNAG 3 cut(s) 386, 746, 874
BstDSI CCRYGG 2 cut(s) 624, 794
BstENI CCTNNNNNAGG 2 cut(s) 220, 400
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 7 cut(s) 7, 293, 308, 319, 442, 608, 817
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 216, 273, 310
BstNI CCWGG 3 cut(s) 29, 199, 522
BstNSI RCATGY 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 4 cut(s) 27, 197, 520, 545
BstSFI CTRYAG 2 cut(s) 181, 217
BstSLI GKGCMC 1 cut(s) 137
BstV1I GCAGC 3 cut(s) 94, 260, 660
BstX2I RGATCY 3 cut(s) 4, 290, 316
BstXI CCANNNNNNTGG 1 cut(s) 628
BstYI RGATCY 3 cut(s) 4, 290, 316
BsuRI GGCC 2 cut(s) 65, 109
BtgI CCRYGG 2 cut(s) 624, 794
BtgZI GCGATG 2 cut(s) 345, 501
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 3 cut(s) 39, 67, 87
CciI TCATGA 1 cut(s) 859
Cfr13I GGNCC 2 cut(s) 231, 543
CpoI CGGWCCG 1 cut(s) 543
Csp6I GTAC 2 cut(s) 202, 226
CspI CGGWCCG 1 cut(s) 543
CviAII CATG 8 cut(s) 58, 417, 451, 625, 795, 832, 860, 917
CviQI GTAC 2 cut(s) 202, 226
DdeI CTNAG 3 cut(s) 386, 746, 874
DpnI GATC 7 cut(s) 6, 292, 307, 318, 441, 607, 816
DpnII GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
DriI GACNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 874
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 874
EciI GGCGGA 1 cut(s) 653
Eco130I CCWWGG 3 cut(s) 104, 624, 794
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 2 cut(s) 231, 543
EcoNI CCTNNNNNAGG 2 cut(s) 220, 400
EcoRII CCWGG 3 cut(s) 27, 197, 520
EcoT14I CCWWGG 3 cut(s) 104, 624, 794
ErhI CCWWGG 3 cut(s) 104, 624, 794
FaeI CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 863, 920
FatI CATG 8 cut(s) 57, 416, 450, 624, 794, 831, 859, 916
FauI CCCGC 1 cut(s) 357
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 5 cut(s) 83, 241, 274, 277, 674
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 5 cut(s) 83, 241, 274, 277, 674
FspBI CTAG 3 cut(s) 53, 402, 932
GluI GCNGC 5 cut(s) 83, 241, 274, 277, 674
GsaI CCCAGC 2 cut(s) 82, 196
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 2 cut(s) 65, 109
HapII CCGG 2 cut(s) 541, 546
Hin1II CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 863, 920
HinfI GANTC 3 cut(s) 146, 374, 777
HpaII CCGG 2 cut(s) 541, 546
HphI GGTGA 3 cut(s) 181, 434, 644
Hpy166II GTNNAC 1 cut(s) 17
Hpy188III TCNNGA 6 cut(s) 409, 530, 541, 580, 860, 876
Hpy8I GTNNAC 1 cut(s) 17
Hpy99I CGWCG 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 7 cut(s) 11, 37, 210, 219, 509, 676, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 273, 310
HpyF3I CTNAG 3 cut(s) 386, 746, 874
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 863, 920
Kpn2I TCCGGA 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
LmnI GCTCC 2 cut(s) 357, 411
Lsp1109I GCAGC 3 cut(s) 94, 260, 660
LweI GCATC 3 cut(s) 518, 523, 524
MaeI CTAG 3 cut(s) 53, 402, 932
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 143, 718, 724
MalI GATC 7 cut(s) 6, 292, 307, 318, 441, 607, 816
MbiI CCGCTC 1 cut(s) 352
MboI GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
MboII GAAGA 2 cut(s) 891, 917
MflI RGATCY 3 cut(s) 4, 290, 316
MhlI GDGCHC 2 cut(s) 137, 856
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 3 cut(s) 46, 472, 689
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 3 cut(s) 140, 368, 786
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 65
MroI TCCGGA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 65
MseI TTAA 1 cut(s) 937
MslI CAYNNNNRTG 4 cut(s) 626, 681, 731, 864
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 2 cut(s) 541, 546
MspR9I CCNGG 4 cut(s) 29, 199, 522, 547
MvaI CCWGG 3 cut(s) 29, 199, 522
MwoI GCNNNNNNNGC 3 cut(s) 216, 273, 310
NciI CCSGG 1 cut(s) 547
NcoI CCATGG 2 cut(s) 624, 794
NdeII GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
NlaIII CATG 8 cut(s) 61, 420, 454, 628, 798, 835, 863, 920
NlaIV GGNNCC 4 cut(s) 6, 407, 551, 619
NmuCI GTSAC 3 cut(s) 143, 718, 724
NspI RCATGY 1 cut(s) 61
OliI CACNNNNGTG 1 cut(s) 626
PagI TCATGA 1 cut(s) 859
PciI ACATGT 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PkrI GCNGC 5 cut(s) 84, 242, 275, 278, 675
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 3 cut(s) 140, 368, 785
PpsI GAGTC 3 cut(s) 140, 368, 785
PscI ACATGT 1 cut(s) 57
PshAI GACNNNNGTC 1 cut(s) 387
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 3 cut(s) 27, 197, 520
PspFI CCCAGC 2 cut(s) 78, 192
PspGI CCWGG 3 cut(s) 27, 197, 520
PspN4I GGNNCC 4 cut(s) 6, 407, 551, 619
PspPI GGNCC 2 cut(s) 231, 543
PstI CTGCAG 1 cut(s) 221
PsuI RGATCY 3 cut(s) 4, 290, 316
PvuI CGATCG 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 2 cut(s) 203, 227
RsaNI GTAC 2 cut(s) 202, 226
RseI CAYNNNNRTG 4 cut(s) 626, 681, 731, 864
Rsr2I CGGWCCG 1 cut(s) 543
RsrII CGGWCCG 1 cut(s) 543
SaqAI TTAA 1 cut(s) 937
SatI GCNGC 5 cut(s) 83, 241, 274, 277, 674
Sau3AI GATC 7 cut(s) 4, 290, 305, 316, 439, 605, 814
Sau96I GGNCC 2 cut(s) 231, 543
SbfI CCTGCAGG 1 cut(s) 221
SchI GAGTC 3 cut(s) 140, 368, 786
ScrFI CCNGG 4 cut(s) 29, 199, 522, 547
SdaI CCTGCAGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 137, 856
SfaNI GCATC 3 cut(s) 518, 523, 524
SfcI CTRYAG 2 cut(s) 181, 217
SinI GGWCC 2 cut(s) 231, 543
SmiMI CAYNNNNRTG 4 cut(s) 626, 681, 731, 864
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse8387I CCTGCAGG 1 cut(s) 221
Sse9I AATT 3 cut(s) 46, 472, 689
SsiI CCGC 7 cut(s) 240, 250, 267, 277, 350, 638, 660
SspMI CTAG 3 cut(s) 53, 402, 932
StyD4I CCNGG 4 cut(s) 27, 197, 520, 545
StyI CCWWGG 3 cut(s) 104, 624, 794
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 299, 494, 555
TasI AATT 3 cut(s) 46, 472, 689
TauI GCSGC 2 cut(s) 243, 279
Tru1I TTAA 1 cut(s) 937
Tru9I TTAA 1 cut(s) 937
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 3 cut(s) 143, 718, 724
TseI GCWGC 3 cut(s) 82, 273, 673
Tsp45I GTSAC 3 cut(s) 143, 718, 724
TspDTI ATGAA 2 cut(s) 749, 818
TspRI CASTG 1 cut(s) 252
VpaK11BI GGWCC 2 cut(s) 231, 543
XagI CCTNNNNNAGG 2 cut(s) 220, 400
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XspI CTAG 3 cut(s) 53, 402, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.