pycom11g11170

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012841
Physical Location & Seq
Forward (+)
9733870 .. 9736409
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGGGAAATTTTGGAAGCACCACCTCCGGGGGTCGCCGGAGGCACCGCCCGCAACCCCAAATGTCTGGGGGCACTCATGTTTTTGCTGCTTCACCACCACCTCATGCATCTCAATATCCCAACGGACCAGACTCCAATCCACCCCAGTACCATCAATACCCGTCTCTATATCCAAATGGATCAAACCCAAATCCGCCCCAGTTCTTCCAATACCCCGGATTCCACCCGCCGCCGCCTGTGCCGGGTCCGTATCCGCAGTCTTTTCACGGCACCGGGGTCTACATGGGCCCCACTCCAGCTAATTGGGTTCACGGGCAGTTCCCTTTCGGTGCACCCCCTCCTCCCCCTCAGGGTGCTCCCTTTGTGGAACATCAGAAGACGGTCACCATTAAGAATGACGTGAATATCAAGAAAGACTCTTTGAGGGTTGAGCCGGATGAGGAGAATCCCGGGAGGTTCGTTGTTGCTTTCACGTTTGATGCTGCTGCGCCCGGAAGCATTACAGTTATGTTTTTTGCTAAAGAAGATGTAGACTGTAAACTGATAGCAACAAAGGAAAGCTTGCTTAAATCTGTTACAGTGCCTTTTGAGAAAGGTCTTGGTCAAAAGTTTAGACAACCTTCCGGAACTGGGATTGACTTCTCGGTGTTTGAGGACATAGGGTTAACAAAAGAGGGTGATGTAGAAGTTTATCCTCTTGTGGTGAAGGCTGAATCCTTCCCCTTGAATGATGGTGAACAGGAATTGGATGGAAATTCCGCTGGGAATTCTCAGATCACCCAGGCGGTGTTTGAAAAGAAGGAGAATGGGGAGTACAAAGTGCGGGTGATGAAGCAGATACTGTGGGTGAATGGTATGAGGTACGAGCTGCAGGAGATCTTTGGTATTGGGAATTCAGTTGAAAATGATCAAAATGGAAATGATTCTGGCAAAGAATGTATAATTTGCTTGTCAGAACCTCGAGACACAACTGTCCTCCCATGCCGGCACATGTGCATGTGCAGTGGTTGTGCCAAGGTTCTGAGGTTCCAGACAAACAGATGCCCCGTCTGTAGGCAACCAGTTGACCGGCTCTTGGAAATCAAGAAACCCCTTGTTTTCCCTTTCATGAGGCGTGGGGCGGATGACTCTGGCGCCACGCTTCAGCTTCCTTGTATTTGA

Protein Analysis

387

Amino Acids

42.48

Weight (kDa)

6.32

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 134 - 290 1.8e-67 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 311 - 357 5e-16 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 312 - 352 1.9e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012841)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53410
fragaria_vesca FvH4_3g32531 FvH4_3g32531
malus_domestica MD03G1116400.v1.1 MD11G1134600.v1.1
prunus_persica Prupe.6G100500_v2.0.a1
pyrus_communis pycom11g11170
rosa_chinensis RchiOBHm_Chr5g0059601
rosa_laevigata RLG00000035307 RLG00000035309
rosa_multiflora Rmu_ssc0000488.1_g000001
rosa_roxburghii Rroxscaffold_1G00021010
rosa_rugosa Rorug05G0323600
rosa_samantha Rh5AG388600 Rh5BG400200 Rh5CG424500 Rh5DG416500
rosa_wichuraiana Rw5G036590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 971
AccB1I GGYRCC 3 cut(s) 42, 269, 1133
AccI GTMKAC 2 cut(s) 279, 531
AccIII TCCGGA 1 cut(s) 623
AclWI GGATC 1 cut(s) 187
AcsI RAATTY 4 cut(s) 7, 754, 766, 892
AcuI CTGAAG 1 cut(s) 1127
AcyI GRCGYC 1 cut(s) 1134
AfaI GTAC 3 cut(s) 149, 815, 863
AfiI CCNNNNNNNGG 6 cut(s) 27, 242, 350, 630, 1053, 1075
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 3 cut(s) 727, 794, 902
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 780
AluBI AGCT 4 cut(s) 299, 561, 868, 1147
AluI AGCT 4 cut(s) 299, 561, 868, 1147
Alw21I GWGCWC 2 cut(s) 334, 358
Alw26I GTCTC 2 cut(s) 168, 957
Alw44I GTGCAC 1 cut(s) 330
AlwI GGATC 1 cut(s) 187
AlwNI CAGNNNCTG 1 cut(s) 841
Ama87I CYCGRG 2 cut(s) 449, 960
Aor13HI TCCGGA 1 cut(s) 623
AoxI GGCC 1 cut(s) 286
ApaI GGGCCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 330
ApeKI GCWGC 4 cut(s) 86, 482, 485, 868
ApoI RAATTY 4 cut(s) 7, 754, 766, 892
Asp700I GAANNNNTTC 1 cut(s) 922
AspLEI GCGC 2 cut(s) 490, 1136
AspS9I GGNCC 4 cut(s) 125, 245, 286, 287
AsuC2I CCSGG 7 cut(s) 28, 216, 243, 274, 450, 451, 492
AsuHPI GGTGA 8 cut(s) 84, 376, 689, 715, 746, 769, 838, 859
AvaI CYCGRG 2 cut(s) 449, 960
AvaII GGWCC 2 cut(s) 125, 245
AxyI CCTNAGG 1 cut(s) 348
BaeGI GKGCMC 3 cut(s) 74, 290, 334
BaeI ACNNNNGTAYC 2 cut(s) 131, 164
BanI GGYRCC 3 cut(s) 42, 269, 1133
BanII GRGCYC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 2 cut(s) 334, 358
BbvI GCAGC 4 cut(s) 73, 469, 472, 855
BccI CCATC 3 cut(s) 159, 725, 743
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 782
BciVI GTATCC 1 cut(s) 261
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 7 cut(s) 28, 216, 243, 274, 450, 451, 492
BcoDI GTCTC 2 cut(s) 168, 957
BfmI CTRYAG 2 cut(s) 869, 1051
BfoI RGCGCY 1 cut(s) 1137
BfuI GTATCC 1 cut(s) 261
BglII AGATCT 1 cut(s) 876
BisI GCNGC 6 cut(s) 87, 230, 233, 483, 486, 869
BlsI GCNGC 6 cut(s) 88, 231, 234, 484, 487, 870
Bme1390I CCNGG 8 cut(s) 28, 216, 243, 274, 450, 451, 492, 782
Bme18I GGWCC 2 cut(s) 125, 245
BmeT110I CYCGRG 2 cut(s) 449, 960
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 4 cut(s) 125, 245, 286, 287
BmiI GGNNCC 7 cut(s) 44, 246, 271, 288, 289, 1028, 1135
BmrFI CCNGG 8 cut(s) 28, 216, 243, 274, 450, 451, 492, 782
BmrI ACTGGG 3 cut(s) 139, 193, 639
BmsI GCATC 3 cut(s) 116, 469, 1031
BmuI ACTGGG 3 cut(s) 139, 193, 639
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 7 cut(s) 28, 216, 243, 274, 450, 451, 492
BsaHI GRCGYC 1 cut(s) 1134
BsaJI CCNNGG 6 cut(s) 27, 214, 273, 449, 780, 1014
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 242, 350, 630, 1053, 1075
Bse118I RCCGGY 2 cut(s) 984, 1068
Bse1I ACTGG 4 cut(s) 145, 199, 634, 1061
Bse21I CCTNAGG 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 623
BseBI CCWGG 1 cut(s) 782
BseDI CCNNGG 6 cut(s) 27, 214, 273, 449, 780, 1014
BseGI GGATG 3 cut(s) 442, 754, 1129
BseLI CCNNNNNNNGG 6 cut(s) 27, 242, 350, 630, 1053, 1075
BseMII CTCAG 3 cut(s) 362, 785, 1013
BseNI ACTGG 4 cut(s) 145, 199, 634, 1061
BseRI GAGGAG 2 cut(s) 330, 455
BseSI GKGCMC 3 cut(s) 74, 290, 334
BseXI GCAGC 4 cut(s) 73, 469, 472, 855
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 1021
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 3 cut(s) 42, 269, 1133
BsiHKAI GWGCWC 2 cut(s) 334, 358
BsiHKCI CYCGRG 2 cut(s) 449, 960
BslI CCNNNNNNNGG 6 cut(s) 27, 242, 350, 630, 1053, 1075
BsmAI GTCTC 2 cut(s) 168, 957
BsmBI CGTCTC 1 cut(s) 168
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 2 cut(s) 449, 960
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 4 cut(s) 74, 290, 334, 358
Bsp13I TCCGGA 1 cut(s) 623
Bsp143I GATC 4 cut(s) 179, 774, 876, 907
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 3 cut(s) 361, 784, 1014
BspEI TCCGGA 1 cut(s) 623
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 7 cut(s) 44, 246, 271, 288, 289, 1028, 1135
BspMAI CTGCAG 1 cut(s) 873
BspPI GGATC 1 cut(s) 187
BspT107I GGYRCC 3 cut(s) 42, 269, 1133
BsrFI RCCGGY 2 cut(s) 984, 1068
BsrI ACTGG 4 cut(s) 145, 199, 634, 1061
BssAI RCCGGY 2 cut(s) 984, 1068
BssECI CCNNGG 6 cut(s) 27, 214, 273, 449, 780, 1014
BssMI GATC 4 cut(s) 179, 774, 876, 907
BssNI GRCGYC 1 cut(s) 1134
BssT1I CCWWGG 1 cut(s) 1014
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 6 cut(s) 382, 505, 536, 580, 843, 973
BstACI GRCGYC 1 cut(s) 1134
BstC8I GCNNGC 3 cut(s) 50, 563, 986
BstDEI CTNAG 3 cut(s) 348, 771, 1022
BstEII GGTNACC 1 cut(s) 382
BstF5I GGATG 3 cut(s) 442, 754, 1129
BstH2I RGCGCY 1 cut(s) 1137
BstHHI GCGC 2 cut(s) 490, 1136
BstKTI GATC 4 cut(s) 182, 777, 879, 910
BstMAI GTCTC 2 cut(s) 168, 957
BstMBI GATC 4 cut(s) 179, 774, 876, 907
BstMWI GCNNNNNNNGC 2 cut(s) 49, 238
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 2 cut(s) 994, 1000
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 8 cut(s) 26, 214, 241, 272, 448, 449, 490, 780
BstSFI CTRYAG 2 cut(s) 869, 1051
BstSLI GKGCMC 3 cut(s) 74, 290, 334
BstV1I GCAGC 4 cut(s) 73, 469, 472, 855
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 1 cut(s) 876
BstXI CCANNNNNNTGG 2 cut(s) 65, 303
BstYI RGATCY 1 cut(s) 876
Bsu36I CCTNAGG 1 cut(s) 348
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 1 cut(s) 288
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 3 cut(s) 442, 754, 1129
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 2 cut(s) 585, 1009
Cac8I GCNNGC 3 cut(s) 50, 563, 986
CaiI CAGNNNCTG 1 cut(s) 841
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 2 cut(s) 490, 1136
Cfr10I RCCGGY 2 cut(s) 984, 1068
Cfr13I GGNCC 4 cut(s) 125, 245, 286, 287
Cfr9I CCCGGG 1 cut(s) 449
Csp6I GTAC 3 cut(s) 148, 814, 862
CviAII CATG 7 cut(s) 77, 104, 283, 981, 991, 997, 1108
CviJI RGCY 8 cut(s) 288, 299, 433, 561, 710, 868, 1072, 1147
CviKI_1 RGCY 8 cut(s) 288, 299, 433, 561, 710, 868, 1072, 1147
CviQI GTAC 3 cut(s) 148, 814, 862
DdeI CTNAG 3 cut(s) 348, 771, 1022
DinI GGCGCC 1 cut(s) 1135
DpnI GATC 4 cut(s) 181, 776, 878, 909
DpnII GATC 4 cut(s) 179, 774, 876, 907
DrdI GACNNNNNNGTC 1 cut(s) 971
DseDI GACNNNNNNGTC 1 cut(s) 971
EciI GGCGGA 2 cut(s) 183, 1136
Eco130I CCWWGG 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 290
Eco47I GGWCC 2 cut(s) 125, 245
Eco57I CTGAAG 1 cut(s) 1127
Eco81I CCTNAGG 1 cut(s) 348
Eco88I CYCGRG 2 cut(s) 449, 960
Eco91I GGTNACC 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 382
EcoRI GAATTC 2 cut(s) 766, 892
EcoRII CCWGG 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 1014
EcoT22I ATGCAT 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 290
EgeI GGCGCC 1 cut(s) 1135
EheI GGCGCC 1 cut(s) 1135
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 7 cut(s) 80, 107, 286, 984, 994, 1000, 1111
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FatI CATG 7 cut(s) 76, 103, 282, 980, 990, 996, 1107
FauI CCCGC 3 cut(s) 57, 234, 816
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 2 cut(s) 279, 531
Fnu4HI GCNGC 6 cut(s) 87, 230, 233, 483, 486, 869
FokI GGATG 3 cut(s) 449, 761, 1136
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 6 cut(s) 87, 230, 233, 483, 486, 869
GlaI GCGC 2 cut(s) 489, 1135
GluI GCNGC 6 cut(s) 87, 230, 233, 483, 486, 869
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 1137
HaeIII GGCC 1 cut(s) 288
HhaI GCGC 2 cut(s) 490, 1136
Hin1I GRCGYC 1 cut(s) 1134
Hin1II CATG 7 cut(s) 80, 107, 286, 984, 994, 1000, 1111
Hin6I GCGC 2 cut(s) 488, 1134
HinP1I GCGC 2 cut(s) 488, 1134
HincII GTYRAC 2 cut(s) 666, 1066
HindII GTYRAC 2 cut(s) 666, 1066
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 7 cut(s) 131, 219, 416, 445, 713, 923, 1127
HpaI GTTAAC 1 cut(s) 666
HphI GGTGA 8 cut(s) 84, 376, 689, 715, 746, 769, 838, 859
Hpy166II GTNNAC 8 cut(s) 280, 310, 332, 532, 539, 666, 737, 1066
Hpy188I TCNGA 4 cut(s) 375, 774, 955, 1023
Hpy188III TCNNGA 6 cut(s) 409, 624, 962, 1030, 1084, 1108
Hpy8I GTNNAC 8 cut(s) 280, 310, 332, 532, 539, 666, 737, 1066
HpyAV CCTTC 4 cut(s) 630, 700, 727, 793
HpyCH4III ACNGT 6 cut(s) 382, 505, 536, 580, 843, 973
HpyCH4IV ACGT 2 cut(s) 399, 473
HpyCH4V TGCA 5 cut(s) 107, 332, 871, 996, 1002
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 238
HpyF3I CTNAG 3 cut(s) 348, 771, 1022
HpySE526I ACGT 2 cut(s) 399, 473
Hsp92I GRCGYC 1 cut(s) 1134
Hsp92II CATG 7 cut(s) 80, 107, 286, 984, 994, 1000, 1111
HspAI GCGC 2 cut(s) 488, 1134
KasI GGCGCC 1 cut(s) 1133
Kpn2I TCCGGA 1 cut(s) 623
KroI GCCGGC 1 cut(s) 984
KroNI GCCGGC 1 cut(s) 986
Ksp22I TGATCA 1 cut(s) 907
KspAI GTTAAC 1 cut(s) 666
Kzo9I GATC 4 cut(s) 179, 774, 876, 907
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 4 cut(s) 73, 469, 472, 855
LweI GCATC 3 cut(s) 116, 469, 1031
MaeII ACGT 2 cut(s) 399, 473
MaeIII GTNAC 2 cut(s) 382, 574
MalI GATC 4 cut(s) 181, 776, 878, 909
MboI GATC 4 cut(s) 179, 774, 876, 907
MboII GAAGA 3 cut(s) 196, 388, 536
MflI RGATCY 1 cut(s) 876
MhlI GDGCHC 4 cut(s) 74, 290, 334, 358
MluCI AATT 7 cut(s) 7, 301, 743, 754, 766, 892, 942
Mly113I GGCGCC 1 cut(s) 1134
MlyI GAGTC 3 cut(s) 125, 410, 1121
Mph1103I ATGCAT 1 cut(s) 109
MroI TCCGGA 1 cut(s) 623
MroNI GCCGGC 1 cut(s) 984
MroXI GAANNNNTTC 1 cut(s) 922
MseI TTAA 3 cut(s) 390, 567, 665
MslI CAYNNNNRTG 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 761
MspR9I CCNGG 8 cut(s) 28, 216, 243, 274, 450, 451, 492, 782
MvaI CCWGG 1 cut(s) 782
MwoI GCNNNNNNNGC 2 cut(s) 49, 238
NaeI GCCGGC 1 cut(s) 986
NarI GGCGCC 1 cut(s) 1134
NciI CCSGG 7 cut(s) 28, 216, 243, 274, 450, 451, 492
NdeII GATC 4 cut(s) 179, 774, 876, 907
NgoMIV GCCGGC 1 cut(s) 984
NlaIII CATG 7 cut(s) 80, 107, 286, 984, 994, 1000, 1111
NlaIV GGNNCC 7 cut(s) 44, 246, 271, 288, 289, 1028, 1135
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 2 cut(s) 994, 1000
PaeR7I CTCGAG 1 cut(s) 960
PagI TCATGA 1 cut(s) 1107
PciI ACATGT 1 cut(s) 990
PdiI GCCGGC 1 cut(s) 986
PdmI GAANNNNTTC 1 cut(s) 922
PfeI GAWTC 4 cut(s) 219, 445, 713, 923
PkrI GCNGC 6 cut(s) 88, 231, 234, 484, 487, 870
PleI GAGTC 3 cut(s) 125, 410, 1121
PluTI GGCGCC 1 cut(s) 1137
PpsI GAGTC 3 cut(s) 125, 410, 1121
PscI ACATGT 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 780
PspEI GGTNACC 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 7 cut(s) 44, 246, 271, 288, 289, 1028, 1135
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 4 cut(s) 125, 245, 286, 287
PstI CTGCAG 1 cut(s) 873
PstNI CAGNNNCTG 1 cut(s) 841
PsuI RGATCY 1 cut(s) 876
RsaI GTAC 3 cut(s) 149, 815, 863
RsaNI GTAC 3 cut(s) 148, 814, 862
RseI CAYNNNNRTG 1 cut(s) 995
SaqAI TTAA 3 cut(s) 390, 567, 665
SatI GCNGC 6 cut(s) 87, 230, 233, 483, 486, 869
Sau3AI GATC 4 cut(s) 179, 774, 876, 907
Sau96I GGNCC 4 cut(s) 125, 245, 286, 287
SchI GAGTC 3 cut(s) 125, 410, 1121
ScrFI CCNGG 8 cut(s) 28, 216, 243, 274, 450, 451, 492, 782
SduI GDGCHC 4 cut(s) 74, 290, 334, 358
SfaNI GCATC 3 cut(s) 116, 469, 1031
SfcI CTRYAG 2 cut(s) 869, 1051
SfoI GGCGCC 1 cut(s) 1135
Sfr274I CTCGAG 1 cut(s) 960
SinI GGWCC 2 cut(s) 125, 245
SlaI CTCGAG 1 cut(s) 960
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 1 cut(s) 960
SmoI CTYRAG 1 cut(s) 960
Sse9I AATT 7 cut(s) 7, 301, 743, 754, 766, 892, 942
SspDI GGCGCC 1 cut(s) 1133
StyD4I CCNGG 8 cut(s) 26, 214, 241, 272, 448, 449, 490, 780
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 6 cut(s) 382, 505, 536, 580, 843, 973
TaiI ACGT 2 cut(s) 402, 476
TaqI TCGA 1 cut(s) 961
TasI AATT 7 cut(s) 7, 301, 743, 754, 766, 892, 942
TatI WGTACW 1 cut(s) 813
TauI GCSGC 2 cut(s) 232, 235
TfiI GAWTC 4 cut(s) 219, 445, 713, 923
Tru1I TTAA 3 cut(s) 390, 567, 665
Tru9I TTAA 3 cut(s) 390, 567, 665
TscAI CASTG 2 cut(s) 585, 1009
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 4 cut(s) 86, 482, 485, 868
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 2 cut(s) 845, 1096
TspGWI ACGGA 2 cut(s) 138, 237
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 2 cut(s) 585, 1009
VneI GTGCAC 1 cut(s) 330
VpaK11BI GGWCC 2 cut(s) 125, 245
XapI RAATTY 4 cut(s) 7, 754, 766, 892
XceI RCATGY 2 cut(s) 994, 1000
XhoI CTCGAG 1 cut(s) 960
XmaI CCCGGG 1 cut(s) 449
XmiI GTMKAC 2 cut(s) 279, 531
XmnI GAANNNNTTC 1 cut(s) 922
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.