pycom11g13820

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006306
Physical Location & Seq
Reverse (-)
13632106 .. 13635864
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 903 bp
ATGGAAAAGGGTAAACGGGAGGTGGTGGTTTCAGCTCTGCAATTCGCTTGCACCGACGACGTCGCCACCAACGTCGCCATCGCCGAAAGGTTGGTTAGAGCTGCTCATGCGAAGGGTGCAAATATCATTCTTATACAGGAACTCTTTGAGGGACATTACTTCTGTCAGGCACAAAGGGAGGACTTCTTTCAGCGAGCAAAGCCATACAAGAGTCATCCAACCATTCTTAGGATGCAGAAAGTTGCCAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTCGAAGAGGCAAATAATGCCCATTACAATTCAATTGCCATTATCGATGCTGATGGGGCAGATCTTGGACTTTATAGGAAGTCCCACATCCCAGACGGACCAGGCTACCAGGAAAAGTTCTACTTCAATCCAGGTGATACTGGATTCAAGGTTTTCAAGACCAAGTTCGCAACTATTGGAGTTGCAATTTGCTGGGATCAATGGTTTCCCGAGGCTGCCCGGGCTTTGGTTCTTCAAGGTGCTGAGATATTGTTTTACCCTACTGCTATTGGTTCCGAACCTCAAGATGGAGGTCTTGATTCTCGTGATCACTGGAGGCGAGTGATGCAAGGGCATGCTGGGGCTAATGTGGTCCCTGTAGTAACTTCAAATCGCATAGGAAAGGAGATAATTGAGACCGAGCATGGAAAGAGTGAGATCACTTTCTACGGTAACTCTTTTATAGCAGGACCCACTGGTGAAATTGTTGAAACTGCTAATGATAAAGAGGAAGCTGTGCTTGTAGCACACTTTGACCTAGACAAAATCAAATGGAAGAGACATAGCTGGGGAGTATTCCGCGACCGCCGCCCAGATTTATACAAGGTGCTACTGACATCAGATGGCAGCAATTCCCCTTTGTGA

Protein Analysis

301

Amino Acids

33.51

Weight (kDa)

6.22

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 12 - 269 1.1e-51 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccII CGCG 1 cut(s) 840
AciI CCGC 3 cut(s) 838, 844, 847
AclWI GGATC 1 cut(s) 483
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 228, 505, 566
AgsI TTSAA 7 cut(s) 312, 406, 427, 436, 515, 648, 749
AjnI CCWGG 3 cut(s) 379, 387, 409
AluBI AGCT 5 cut(s) 35, 101, 276, 773, 825
AluI AGCT 5 cut(s) 35, 101, 276, 773, 825
Alw26I GTCTC 2 cut(s) 668, 811
AlwI GGATC 1 cut(s) 483
Ama87I CYCGRG 2 cut(s) 488, 498
ApeKI GCWGC 3 cut(s) 101, 494, 885
AspS9I GGNCC 3 cut(s) 377, 631, 728
AsuC2I CCSGG 2 cut(s) 499, 500
AsuHPI GGTGA 2 cut(s) 425, 749
AsuII TTCGAA 1 cut(s) 282
AvaI CYCGRG 2 cut(s) 488, 498
AvaII GGWCC 3 cut(s) 377, 631, 728
BauI CACGAG 1 cut(s) 582
BbvI GCAGC 3 cut(s) 88, 481, 897
BccI CCATC 4 cut(s) 86, 326, 560, 875
BciT130I CCWGG 3 cut(s) 381, 389, 411
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 2 cut(s) 499, 500
BcoDI GTCTC 2 cut(s) 668, 811
BfaI CTAG 1 cut(s) 797
BfmI CTRYAG 1 cut(s) 636
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 102, 495, 847, 886
BlsI GCNGC 4 cut(s) 103, 496, 848, 887
Bme1390I CCNGG 5 cut(s) 381, 389, 411, 499, 500
Bme18I GGWCC 3 cut(s) 377, 631, 728
BmeT110I CYCGRG 2 cut(s) 488, 498
BmgT120I GGNCC 3 cut(s) 377, 631, 728
BmiI GGNNCC 3 cut(s) 553, 633, 730
BmrFI CCNGG 5 cut(s) 381, 389, 411, 499, 500
BmsI GCATC 3 cut(s) 222, 316, 594
BpmI CTGGAG 1 cut(s) 613
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 546
BpuMI CCSGG 2 cut(s) 499, 500
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 2 cut(s) 489, 498
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 505, 566
Bse1I ACTGG 4 cut(s) 269, 424, 596, 739
BseBI CCWGG 3 cut(s) 381, 389, 411
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 489, 498
BseGI GGATG 3 cut(s) 214, 237, 366
BseLI CCNNNNNNNGG 3 cut(s) 228, 505, 566
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 4 cut(s) 269, 424, 596, 739
BseXI GCAGC 3 cut(s) 88, 481, 897
BseYI CCCAGC 3 cut(s) 471, 617, 825
Bsh1236I CGCG 1 cut(s) 840
Bsh1285I CGRYCG 1 cut(s) 844
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 844
BsiHKCI CYCGRG 2 cut(s) 488, 498
BsiSI CCGG 1 cut(s) 499
BslFI GGGAC 3 cut(s) 165, 346, 617
BslI CCNNNNNNNGG 3 cut(s) 228, 505, 566
BsmAI GTCTC 2 cut(s) 668, 811
BsmFI GGGAC 3 cut(s) 165, 346, 617
Bso31I GGTCTC 1 cut(s) 668
BsoBI CYCGRG 2 cut(s) 488, 498
Bsp119I TTCGAA 1 cut(s) 282
Bsp143I GATC 4 cut(s) 340, 475, 586, 696
BspACI CCGC 3 cut(s) 838, 844, 847
BspCNI CTCAG 1 cut(s) 514
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 1 cut(s) 840
BspLI GGNNCC 3 cut(s) 553, 633, 730
BspPI GGATC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 668
BsrI ACTGG 4 cut(s) 269, 424, 596, 739
BssECI CCNNGG 2 cut(s) 489, 498
BssMI GATC 4 cut(s) 340, 475, 586, 696
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 381, 389, 411
Bst4CI ACNGT 1 cut(s) 710
Bst6I CTCTTC 2 cut(s) 279, 809
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 49, 195, 615
BstDEI CTNAG 2 cut(s) 227, 522
BstF5I GGATG 3 cut(s) 214, 237, 366
BstFNI CGCG 1 cut(s) 840
BstKTI GATC 4 cut(s) 343, 478, 589, 699
BstMAI GTCTC 2 cut(s) 668, 811
BstMBI GATC 4 cut(s) 340, 475, 586, 696
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 8 cut(s) 107, 116, 199, 296, 335, 500, 604, 846
BstNI CCWGG 3 cut(s) 381, 389, 411
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 5 cut(s) 379, 387, 409, 497, 498
BstSFI CTRYAG 1 cut(s) 636
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 3 cut(s) 88, 481, 897
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 324
BsuTUI ATCGAT 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 214, 237, 366
BtsIMutI CAGTG 2 cut(s) 589, 732
Cac8I GCNNGC 3 cut(s) 49, 195, 615
Cfr13I GGNCC 3 cut(s) 377, 631, 728
Cfr9I CCCGGG 1 cut(s) 498
ClaI ATCGAT 1 cut(s) 324
CviAII CATG 3 cut(s) 107, 614, 683
DdeI CTNAG 2 cut(s) 227, 522
DpnI GATC 4 cut(s) 342, 477, 588, 698
DpnII GATC 4 cut(s) 340, 475, 586, 696
Eam1104I CTCTTC 2 cut(s) 279, 809
EarI CTCTTC 2 cut(s) 279, 809
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 3 cut(s) 377, 631, 728
Eco88I CYCGRG 2 cut(s) 488, 498
EcoO109I RGGNCCY 1 cut(s) 728
EcoRII CCWGG 3 cut(s) 379, 387, 409
FaeI CATG 3 cut(s) 110, 617, 686
FalI AAGNNNNNCTT 4 cut(s) 386, 418, 762, 794
FaqI GGGAC 3 cut(s) 165, 346, 617
FatI CATG 3 cut(s) 106, 613, 682
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 4 cut(s) 102, 495, 847, 886
FokI GGATG 3 cut(s) 201, 244, 353
Fsp4HI GCNGC 4 cut(s) 102, 495, 847, 886
FspBI CTAG 1 cut(s) 797
GluI GCNGC 4 cut(s) 102, 495, 847, 886
GsaI CCCAGC 3 cut(s) 475, 621, 829
GsuI CTGGAG 1 cut(s) 613
HapII CCGG 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 110, 617, 686
HinfI GANTC 3 cut(s) 211, 423, 578
HpaII CCGG 1 cut(s) 499
HphI GGTGA 2 cut(s) 425, 749
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 556, 880
Hpy188III TCNNGA 5 cut(s) 436, 488, 563, 575, 584
Hpy8I GTNNAC 1 cut(s) 14
Hpy99I CGWCG 4 cut(s) 59, 62, 65, 77
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 2 cut(s) 60, 72
HpyCH4V TGCA 6 cut(s) 40, 51, 119, 235, 464, 607
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 116, 199, 296, 335, 500, 604, 846
HpyF3I CTNAG 2 cut(s) 227, 522
HpySE526I ACGT 2 cut(s) 60, 72
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 110, 617, 686
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 4 cut(s) 340, 475, 586, 696
Lsp1109I GCAGC 3 cut(s) 88, 481, 897
LweI GCATC 3 cut(s) 222, 316, 594
MaeI CTAG 1 cut(s) 797
MaeII ACGT 2 cut(s) 60, 72
MaeIII GTNAC 2 cut(s) 640, 710
MalI GATC 4 cut(s) 342, 477, 588, 698
MboI GATC 4 cut(s) 340, 475, 586, 696
MboII GAAGA 3 cut(s) 296, 503, 826
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 340
MluCI AATT 7 cut(s) 41, 307, 312, 465, 669, 741, 889
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 9 cut(s) 13, 142, 172, 280, 484, 563, 570, 588, 760
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 5 cut(s) 381, 389, 411, 499, 500
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 3 cut(s) 381, 389, 411
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 8 cut(s) 107, 116, 199, 296, 335, 500, 604, 846
NciI CCSGG 2 cut(s) 499, 500
NdeII GATC 4 cut(s) 340, 475, 586, 696
NlaIII CATG 3 cut(s) 110, 617, 686
NlaIV GGNNCC 3 cut(s) 553, 633, 730
NspI RCATGY 1 cut(s) 617
NspV TTCGAA 1 cut(s) 282
PaeI GCATGC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 69, 81
PfeI GAWTC 2 cut(s) 423, 578
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 4 cut(s) 103, 496, 848, 887
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 3 cut(s) 379, 387, 409
PspFI CCCAGC 3 cut(s) 471, 617, 825
PspGI CCWGG 3 cut(s) 379, 387, 409
PspN4I GGNNCC 3 cut(s) 553, 633, 730
PspPI GGNCC 3 cut(s) 377, 631, 728
PspPPI RGGWCCY 1 cut(s) 728
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 59
SatI GCNGC 4 cut(s) 102, 495, 847, 886
Sau3AI GATC 4 cut(s) 340, 475, 586, 696
Sau96I GGNCC 3 cut(s) 377, 631, 728
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 5 cut(s) 381, 389, 411, 499, 500
SfaNI GCATC 3 cut(s) 222, 316, 594
SfcI CTRYAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 282
SinI GGWCC 3 cut(s) 377, 631, 728
SmaI CCCGGG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SphI GCATGC 1 cut(s) 617
SrfI GCCCGGGC 1 cut(s) 500
Sse9I AATT 7 cut(s) 41, 307, 312, 465, 669, 741, 889
SsiI CCGC 3 cut(s) 838, 844, 847
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 5 cut(s) 379, 387, 409, 497, 498
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 2 cut(s) 63, 75
TaqI TCGA 2 cut(s) 282, 324
TaqII GACCGA 1 cut(s) 692
TasI AATT 7 cut(s) 41, 307, 312, 465, 669, 741, 889
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 2 cut(s) 423, 578
TscAI CASTG 2 cut(s) 596, 739
TseI GCWGC 3 cut(s) 101, 494, 885
TspGWI ACGGA 1 cut(s) 390
TspMI CCCGGG 1 cut(s) 498
TspRI CASTG 2 cut(s) 596, 739
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 3 cut(s) 377, 631, 728
XceI RCATGY 1 cut(s) 617
XmaI CCCGGG 1 cut(s) 498
XspI CTAG 1 cut(s) 797
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.