pycom11g19520

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007352
Physical Location & Seq
Reverse (-)
22048910 .. 22049743
834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGAGGAGGAGACCTGGAATCGGCGGCTTGCAGACCGCTGCCGCCGCTAGGGACCAATATCGTCAGCTGGGCGATAATGTTGCCAAGATCAGGACCGATCTCATGAAGGAGCAGCTCACCACTTTCCGAACCCAGTTGGAAGATTTCGCTCGAAAGCACAAGAATGACATTCGAAAGAACCCAGCTTTCAGATCACAATTTCATAACATGTGCACCAAGGTAGGAATCGATCCATTGGCATCCAATAAGGGCTTTTGGGCAGAGCTTTTGGGGATTGGTGATTTCTACTATGAACTTGGGGTGCAAATTGTTGACATTTGCTTGGCAACAAGACCCCACAATGGGGGTTTGATCGAACCTGCAGGAACTATGCAGTCTGCTTCGTCAGAGGCGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

134

Amino Acids

14.88

Weight (kDa)

8.64

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 119 1.8e-30 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
AciI CCGC 5 cut(s) 24, 36, 42, 45, 392
AclWI GGATC 1 cut(s) 224
AfiI CCNNNNNNNGG 6 cut(s) 20, 48, 90, 341, 342, 343
AflIII ACRYGT 1 cut(s) 207
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 4 cut(s) 67, 115, 185, 265
AluI AGCT 4 cut(s) 67, 115, 185, 265
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 4
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 38, 112
AspS9I GGNCC 2 cut(s) 52, 93
AsuHPI GGTGA 2 cut(s) 109, 290
AsuII TTCGAA 1 cut(s) 172
AvaII GGWCC 2 cut(s) 52, 93
BaeGI GKGCMC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 25, 124
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 1 cut(s) 360
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 5 cut(s) 25, 39, 42, 45, 113
BlsI GCNGC 5 cut(s) 26, 40, 43, 46, 114
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 52, 93
BmgT120I GGNCC 2 cut(s) 52, 93
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 1 cut(s) 15
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 172
Bsa29I ATCGAT 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 48, 90, 341, 342, 343
Bse1I ACTGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 15
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 239
BseLI CCNNNNNNNGG 6 cut(s) 20, 48, 90, 341, 342, 343
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 25, 124
BseYI CCCAGC 2 cut(s) 67, 181
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 6 cut(s) 20, 48, 90, 341, 342, 343
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 4
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 5 cut(s) 87, 97, 191, 229, 351
BspACI CCGC 5 cut(s) 24, 36, 42, 45, 392
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 53
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 5 cut(s) 87, 97, 191, 229, 351
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 15
Bst6I CTCTTC 1 cut(s) 390
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 29
BstF5I GGATG 1 cut(s) 239
BstKTI GATC 5 cut(s) 90, 100, 194, 232, 354
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 5 cut(s) 87, 97, 191, 229, 351
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 25, 124
Bsu15I ATCGAT 1 cut(s) 228
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 1 cut(s) 239
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 1 cut(s) 29
CciI TCATGA 1 cut(s) 102
Cfr13I GGNCC 2 cut(s) 52, 93
ClaI ATCGAT 1 cut(s) 228
CviAII CATG 2 cut(s) 103, 208
CviJI RGCY 6 cut(s) 27, 67, 115, 185, 252, 265
CviKI_1 RGCY 6 cut(s) 27, 67, 115, 185, 252, 265
DpnI GATC 5 cut(s) 89, 99, 193, 231, 353
DpnII GATC 5 cut(s) 87, 97, 191, 229, 351
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 2 cut(s) 52, 93
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 106, 211
FaiI YATR 5 cut(s) 104, 204, 209, 291, 371
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 102, 207
Fnu4HI GCNGC 5 cut(s) 25, 39, 42, 45, 113
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 5 cut(s) 25, 39, 42, 45, 113
FspBI CTAG 1 cut(s) 48
GluI GCNGC 5 cut(s) 25, 39, 42, 45, 113
GsaI CCCAGC 2 cut(s) 71, 185
Hin1II CATG 2 cut(s) 106, 211
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 2 cut(s) 18, 225
HphI GGTGA 2 cut(s) 109, 290
Hpy166II GTNNAC 2 cut(s) 213, 313
Hpy188I TCNGA 3 cut(s) 128, 191, 388
Hpy188III TCNNGA 2 cut(s) 91, 103
Hpy8I GTNNAC 2 cut(s) 213, 313
HpyAV CCTTC 1 cut(s) 100
HpyCH4V TGCA 5 cut(s) 31, 213, 304, 362, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 106, 211
Kzo9I GATC 5 cut(s) 87, 97, 191, 229, 351
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 7 cut(s) 27, 53, 76, 146, 195, 348, 372
Lsp1109I GCAGC 2 cut(s) 25, 124
LweI GCATC 1 cut(s) 248
MaeI CTAG 1 cut(s) 48
MalI GATC 5 cut(s) 89, 99, 193, 231, 353
MboI GATC 5 cut(s) 87, 97, 191, 229, 351
MboII GAAGA 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 2 cut(s) 197, 306
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 1 cut(s) 382
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 2 cut(s) 38, 67
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 5 cut(s) 87, 97, 191, 229, 351
NlaIII CATG 2 cut(s) 106, 211
NlaIV GGNNCC 1 cut(s) 53
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 172
PagI TCATGA 1 cut(s) 102
PciI ACATGT 1 cut(s) 207
PfeI GAWTC 2 cut(s) 18, 225
PkrI GCNGC 5 cut(s) 26, 40, 43, 46, 114
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 2 cut(s) 67, 181
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 2 cut(s) 52, 93
PstI CTGCAG 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 162
SatI GCNGC 5 cut(s) 25, 39, 42, 45, 113
Sau3AI GATC 5 cut(s) 87, 97, 191, 229, 351
Sau96I GGNCC 2 cut(s) 52, 93
SbfI CCTGCAGG 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 364
SduI GDGCHC 1 cut(s) 215
SetI ASST 7 cut(s) 16, 69, 117, 187, 222, 267, 361
SfaNI GCATC 1 cut(s) 248
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 172
SinI GGWCC 2 cut(s) 52, 93
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse8387I CCTGCAGG 1 cut(s) 364
Sse9I AATT 2 cut(s) 197, 306
SsiI CCGC 5 cut(s) 24, 36, 42, 45, 392
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 216
TaqI TCGA 4 cut(s) 151, 172, 228, 354
TaqII GACCGA 1 cut(s) 110
TasI AATT 2 cut(s) 197, 306
TauI GCSGC 3 cut(s) 27, 44, 47
TfiI GAWTC 2 cut(s) 18, 225
TseI GCWGC 2 cut(s) 38, 112
TspDTI ATGAA 3 cut(s) 119, 191, 306
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 93
XceI RCATGY 1 cut(s) 211
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.