pycom11g22780

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010541
Physical Location & Seq
Reverse (-)
25807305 .. 25811060
3756 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2619 bp
ATGTCTCAGTTTGCTCTTCTTTTTTATTTCCTGATCACAGTTTCAGGTTTTAGGGAAGGAATTACAACATTTGGAATTGCCAATACAATCGAGAATGAATCCGGGATGGGAATACTAGGTGCTATTATCGACAACAGTTCTCGGATTGGCAAAGAAGAGAGAGTGGCAATTCAAATGGCACTGGATGATCATTTTGATTCCACTGGGAATCAGAGACTGGTTCTGCACGTAAGAAACTCTCAGAGGAAGCCCTTGCAGGCAGCTCTTGCAGCTAAAGGTCTTATAGACGAGCAAAAAGTGCAAGCCATTCTAGGACCTCATACATGGGAAGAGACGTCATTAGTAGCTGAGGTTGGCAGTGAGACTCATACCCCAATTGTTTCATTTGCAGAGGCTAATCCAATATGGGCAGCTGAGCTGTGGCCCTTTCTGGTTCAAGCTTCCCGTAACCGGTTGAAACAAATGGAAGCAATTGCCGATATAGTTCAGTCTTGGGAATGGCATCAAGTCACTGTAATTTACGAAGATAGAGATTCTTCAGCCAATGAAGTCTTACCTCACCTCTCCGATGCCTTGAGACAAGTTGGTGCAGAAATTAGCCACCTGGTAGCTCTCCCTCCATTTGCTTCTTCTTCATTAATCACAGAGCTTGAGAAGATTCAAAACGACCAATGTAGAGTCTTTGTTGTGCATTTGTCTGCGCCATTGGCGGTGCAACTATTCGAAAAGGCAAAAGAAATGAAGATGATGGAAAAGGATTATGTATGGATCACCACTGATCCTATTACAAGCCTTGTCCACTCTTTTAATGCCTCCACCAGCTCCTCAATGCAAGGGATCATCGGAGTCAAGAGCTACTTCCCCGAAAGTGGGAACCAATTTAAGGACTTCGATCATAGGTTTCGAAAAAGGTTTAGTACGAAACATCCTGAAGACGGTAACAATGAGCCTGGAATCTTTGCAGCACAGGCTTATGATGCTGCAAGGGCAGTGGCTCAAGCTATGACAGAAAGCAGCAAAGGAAGGAAATTGTTAGCAAAACTTTTGCGAAGTGATTTTCATGGCTTAAGTGGAAGAATTAAGTTCAATGGCCAAAAATTAGCTCCGCAACATGTATTTCAAATCATCAATGTGAATGGAAAAAGTTACAGAGAAGTTGGATACTGGTCAGATGGATTAGGATTCTCAGAGACTATCGGTGCAGGTGCTACTAACAGATCTTCTATGAGATATTTGGGGCAAGTGTTTTGGCCAGGAGGGCCTTGGTATACTCCAAAAGGGTGGGCTGTACCAAGAACAAATGCAAACGTGCTGAGAATTGGTGTTCCGACAGGATCAACATTCAAACAGTATGTAAATGTGAAGAAAGATCCATTGGGAAATAACTTTTCTTTCAGTGGAATTGCAATAGATCTCTTCAAAACAACCGTAAATGAGCTGCCTTACCATTTGCCCTACGAGTTCTTTCCTTTCGAAGGAACGTATGATTCTTTGGTGAAGCAAATCCATTTGCATAAATTTGATGCTGTGGTTGGTGATGTAGCAATAGTATCCCGGAGATATGAACACGCAGAATTCACGCAACCCTATACCGAATCAGGATTGATAATGATAGTTCCAGTCAGGTCCAAAACAAGTAACAAAGCTTGGTTATTCATGAAACCTTTCACAAAAGCAATGTGGGCACTTATAGGAGCCATCAATGTCTACAATGGCTTTGTTGTATGGCTGATAGAGCGAAACTACCGTCCCGAACTCAAAGGTTCTGTGCTAAATCAAATAGGAAGCTTGATTTGGCTATCTTTCACCACTCTCTTCTCCTTACAAGGAGATAAGCTGCATAGCAATTTGTCTCGGATGACAATGGTGATCTGGCTATTTATGGCGCTAATCATCACGCAGACATACACCGCTAACCTTGCCAGCATGCTCACTGTCCAGCAGCTTGAACCTACCGTAGCAGATGTTGATGAACTGCTGCGAACCAACGCCATGGTTGGTTATTGTAAGGGATCCTTTATTTCAGCCTATCTAACGGAAGTCTTGCACTTCCACCACAACAATATTATGCAGTTTAACTCCATAGAAGAATATGCCTTAGCCCTCAGACGTAAAGTAATTGCTGCTGCTTTTCTCGACGCCCCTCTGGCAAAAGTATTCCTTGGAAAATACTGCAAAGCCTTTATGGCAGTGGGACCGACTTTCAAAGTTGGAGGATTTGGATTTGTATTTCCAAAAGGGTCTATGTTGCTACCAAGTGTAACGGAAGCAATGTTAAAGGTAACCGAAAGGGGTGACCTTCTGACTTTAGAGAACAACATGCTGGCCTCTCTGAAGTGCTTGGATGCAGAAACAGATGGTGCTAATCCTAGTCTAAGTCCCAACAGCTTTTGGGTACTATTCGTACTCACAGGAGGGACATCAACAAGTGCTCTTGTGGTTTACATTTTTCGCATTAGTAAATCAATTCTTGTAGACAAAACCAACTGGAAGTTAATATTGGATGTAATGAAACGATGGGGAAATCCAAACAGAAGGTGCTCAAGAAAAGACAGCGATATTAATGAAGAAAGTCATCCATATCCTTCGAATGCATCTGCCTTGCAGGCTCAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

873

Amino Acids

97.45

Weight (kDa)

8.46

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 48 - 379 3e-26 Periplasmic binding protein
ANF_receptor PF01094 54 - 380 1.2e-53 Receptor family ligand binding region
SBP_bac_3 PF00497 462 - 770 6.1e-07 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 558 - 806 2.8e-24 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1193
AatII GACGTC 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 1193
AccI GTMKAC 3 cut(s) 1268, 1707, 2475
AciI CCGC 3 cut(s) 710, 1106, 1911
AclWI GGATC 7 cut(s) 773, 776, 845, 1342, 1364, 2007, 2020
AcoI YGGCCR 2 cut(s) 1090, 1250
AcsI RAATTY 2 cut(s) 1517, 1574
AcuI CTGAAG 3 cut(s) 522, 951, 2354
AcyI GRCGYC 2 cut(s) 335, 2139
AfaI GTAC 4 cut(s) 919, 1290, 2397, 2406
AfiI CCNNNNNNNGG 6 cut(s) 450, 869, 870, 883, 935, 1475
AflII CTTAAG 1 cut(s) 1066
AflIII ACRYGT 1 cut(s) 1111
AgeI ACCGGT 1 cut(s) 450
AjnI CCWGG 3 cut(s) 603, 949, 1252
AjuI GAANNNNNNNTTGG 6 cut(s) 1358, 1390, 1777, 1809, 2483, 2515
Alw21I GWGCWC 2 cut(s) 2434, 2543
Alw26I GTCTC 7 cut(s) 9, 208, 326, 356, 571, 1184, 1857
AlwI GGATC 7 cut(s) 773, 776, 845, 1342, 1364, 2007, 2020
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 5 cut(s) 422, 1090, 1250, 1259, 2325
ApoI RAATTY 2 cut(s) 1517, 1574
ArsI GACNNNNNNTTYG 2 cut(s) 1732, 1764
AseI ATTAAT 2 cut(s) 638, 2562
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 1664
AspLEI GCGC 2 cut(s) 703, 1888
AspS9I GGNCC 5 cut(s) 314, 423, 1259, 1626, 2195
AsuC2I CCSGG 2 cut(s) 103, 1555
AsuHPI GGTGA 7 cut(s) 551, 763, 1507, 1547, 1798, 1879, 2306
AsuII TTCGAA 4 cut(s) 723, 904, 1473, 2588
AvaII GGWCC 3 cut(s) 314, 1626, 2195
BaeGI GKGCMC 1 cut(s) 1687
BalI TGGCCA 2 cut(s) 1092, 1252
BamHI GGATCC 1 cut(s) 2012
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 2 cut(s) 2434, 2543
BbvCI CCTCAGC 1 cut(s) 348
BccI CCATC 6 cut(s) 100, 742, 1166, 1706, 2351, 2511
BciT130I CCWGG 3 cut(s) 605, 951, 1254
BciVI GTATCC 2 cut(s) 1154, 1561
BclI TGATCA 2 cut(s) 33, 187
BcnI CCSGG 2 cut(s) 103, 1555
BcoDI GTCTC 7 cut(s) 9, 208, 326, 356, 571, 1184, 1857
BfaI CTAG 4 cut(s) 116, 311, 2370, 2617
BfoI RGCGCY 1 cut(s) 1889
BfrI CTTAAG 1 cut(s) 1066
BfuAI ACCTGC 1 cut(s) 1193
BfuI GTATCC 2 cut(s) 1154, 1561
BglI GCCNNNNNGGC 4 cut(s) 1258, 2147, 2186, 2606
BglII AGATCT 2 cut(s) 1217, 1411
BlpI GCTNAGC 1 cut(s) 414
Bme1390I CCNGG 5 cut(s) 103, 605, 951, 1254, 1555
Bme18I GGWCC 3 cut(s) 314, 1626, 2195
BmgT120I GGNCC 5 cut(s) 314, 423, 1259, 1626, 2195
BmiI GGNNCC 4 cut(s) 875, 1696, 2014, 2196
BmrFI CCNGG 5 cut(s) 103, 605, 951, 1254, 1555
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 6 cut(s) 511, 559, 967, 1513, 2335, 2603
BmuI ACTGGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 939
Bpu10I CCTNAGC 2 cut(s) 348, 2098
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 4 cut(s) 723, 904, 1473, 2588
BpuEI CTTGAG 5 cut(s) 595, 671, 981, 2527, 2595
BpuMI CCSGG 2 cut(s) 103, 1555
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 2 cut(s) 335, 2139
BsaJI CCNNGG 3 cut(s) 1262, 1992, 2161
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 6 cut(s) 450, 869, 870, 883, 935, 1475
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 6 cut(s) 186, 208, 222, 1169, 1619, 2492
Bse3DI GCAATG 2 cut(s) 1683, 2277
BseBI CCWGG 3 cut(s) 605, 951, 1254
BseDI CCNNGG 3 cut(s) 1262, 1992, 2161
BseGI GGATG 7 cut(s) 111, 190, 925, 1863, 2350, 2509, 2575
BseLI CCNNNNNNNGG 6 cut(s) 450, 869, 870, 883, 935, 1475
BseMI GCAATG 2 cut(s) 1683, 2277
BseMII CTCAG 7 cut(s) 20, 254, 339, 405, 1200, 1304, 2119
BseNI ACTGG 6 cut(s) 186, 208, 222, 1169, 1619, 2492
BseRI GAGGAG 1 cut(s) 814
BseSI GKGCMC 1 cut(s) 1687
BsgI GTGCAG 3 cut(s) 209, 609, 1221
BshFI GGCC 5 cut(s) 424, 1092, 1252, 1261, 2327
BshTI ACCGGT 1 cut(s) 450
BsiHKAI GWGCWC 2 cut(s) 2434, 2543
BsiSI CCGG 3 cut(s) 102, 451, 1555
BslFI GGGAC 4 cut(s) 1734, 2208, 2364, 2431
BslI CCNNNNNNNGG 6 cut(s) 450, 869, 870, 883, 935, 1475
BsmAI GTCTC 7 cut(s) 9, 208, 326, 356, 571, 1184, 1857
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 4 cut(s) 1734, 2208, 2364, 2431
BsmI GAATGC 1 cut(s) 2596
BsnI GGCC 5 cut(s) 424, 1092, 1252, 1261, 2327
Bsp119I TTCGAA 4 cut(s) 723, 904, 1473, 2588
Bsp1286I GDGCHC 3 cut(s) 1687, 2434, 2543
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 1 cut(s) 1992
BspACI CCGC 3 cut(s) 710, 1106, 1911
BspANI GGCC 5 cut(s) 424, 1092, 1252, 1261, 2327
BspCNI CTCAG 7 cut(s) 19, 253, 340, 406, 1199, 1305, 2118
BspHI TCATGA 1 cut(s) 1656
BspLI GGNNCC 4 cut(s) 875, 1696, 2014, 2196
BspMI ACCTGC 1 cut(s) 1193
BspPI GGATC 7 cut(s) 773, 776, 845, 1342, 1364, 2007, 2020
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 4 cut(s) 723, 904, 1473, 2588
BspTI CTTAAG 1 cut(s) 1066
BsrDI GCAATG 2 cut(s) 1683, 2277
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 6 cut(s) 186, 208, 222, 1169, 1619, 2492
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 3 cut(s) 1262, 1992, 2161
BssNAI GTATAC 1 cut(s) 1269
BssNI GRCGYC 2 cut(s) 335, 2139
BssT1I CCWWGG 3 cut(s) 1262, 1992, 2161
Bst1107I GTATAC 1 cut(s) 1269
Bst2UI CCWGG 3 cut(s) 605, 951, 1254
Bst4CI ACNGT 9 cut(s) 40, 137, 514, 938, 1350, 1429, 1748, 1936, 1957
Bst6I CTCTTC 5 cut(s) 21, 150, 324, 1421, 1820
BstACI GRCGYC 2 cut(s) 335, 2139
BstAFI CTTAAG 1 cut(s) 1066
BstAPI GCANNNNNTGC 2 cut(s) 266, 298
BstBAI YACGTR 1 cut(s) 229
BstBI TTCGAA 4 cut(s) 723, 904, 1473, 2588
BstC8I GCNNGC 6 cut(s) 258, 303, 1924, 1928, 2325, 2607
BstDEI CTNAG 9 cut(s) 6, 240, 348, 414, 1186, 1313, 2098, 2105, 2375
BstDSI CCRYGG 1 cut(s) 1992
BstEII GGTNACC 2 cut(s) 2281, 2294
BstENI CCTNNNNNAGG 1 cut(s) 1473
BstF5I GGATG 7 cut(s) 111, 190, 925, 1863, 2350, 2509, 2575
BstH2I RGCGCY 1 cut(s) 1889
BstHHI GCGC 2 cut(s) 703, 1888
BstMAI GTCTC 7 cut(s) 9, 208, 326, 356, 571, 1184, 1857
BstNI CCWGG 3 cut(s) 605, 951, 1254
BstNSI RCATGY 3 cut(s) 1115, 1930, 2323
BstPI GGTNACC 2 cut(s) 2281, 2294
BstSCI CCNGG 5 cut(s) 101, 603, 949, 1252, 1553
BstSLI GKGCMC 1 cut(s) 1687
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 4 cut(s) 1217, 1369, 1411, 2012
BstXI CCANNNNNNTGG 2 cut(s) 1281, 1993
BstYI RGATCY 4 cut(s) 1217, 1369, 1411, 2012
BstZ17I GTATAC 1 cut(s) 1269
BsuI GTATCC 2 cut(s) 1154, 1561
BsuRI GGCC 5 cut(s) 424, 1092, 1252, 1261, 2327
BtgI CCRYGG 1 cut(s) 1992
BtsCI GGATG 7 cut(s) 111, 190, 925, 1863, 2350, 2509, 2575
BtsI GCAGTG 3 cut(s) 364, 996, 2196
BtsIMutI CAGTG 9 cut(s) 179, 201, 364, 510, 774, 996, 1402, 1932, 2196
BveI ACCTGC 1 cut(s) 1193
Cac8I GCNNGC 6 cut(s) 258, 303, 1924, 1928, 2325, 2607
CaiI CAGNNNCTG 1 cut(s) 217
CciI TCATGA 1 cut(s) 1656
CfoI GCGC 2 cut(s) 703, 1888
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 5 cut(s) 314, 423, 1259, 1626, 2195
CseI GACGC 1 cut(s) 2147
CsiI ACCWGGT 1 cut(s) 603
Csp6I GTAC 4 cut(s) 918, 1289, 2396, 2405
CspAI ACCGGT 1 cut(s) 450
CspCI CAANNNNNGTGG 2 cut(s) 972, 1007
CviAII CATG 7 cut(s) 324, 1061, 1112, 1657, 1927, 1993, 2320
CviQI GTAC 4 cut(s) 918, 1289, 2396, 2405
DdeI CTNAG 9 cut(s) 6, 240, 348, 414, 1186, 1313, 2098, 2105, 2375
EaeI YGGCCR 2 cut(s) 1090, 1250
Eam1104I CTCTTC 5 cut(s) 21, 150, 324, 1421, 1820
EarI CTCTTC 5 cut(s) 21, 150, 324, 1421, 1820
Eco130I CCWWGG 3 cut(s) 1262, 1992, 2161
Eco47I GGWCC 3 cut(s) 314, 1626, 2195
Eco57I CTGAAG 3 cut(s) 522, 951, 2354
Eco91I GGTNACC 2 cut(s) 2281, 2294
EcoNI CCTNNNNNAGG 1 cut(s) 1473
EcoO109I RGGNCCY 2 cut(s) 314, 1259
EcoO65I GGTNACC 2 cut(s) 2281, 2294
EcoRI GAATTC 1 cut(s) 1574
EcoRII CCWGG 3 cut(s) 603, 949, 1252
EcoT14I CCWWGG 3 cut(s) 1262, 1992, 2161
EcoT22I ATGCAT 1 cut(s) 2596
ErhI CCWWGG 3 cut(s) 1262, 1992, 2161
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 7 cut(s) 327, 1064, 1115, 1660, 1930, 1996, 2323
FalI AAGNNNNNCTT 6 cut(s) 842, 874, 2000, 2032, 2145, 2177
FaqI GGGAC 4 cut(s) 1734, 2208, 2364, 2431
FatI CATG 7 cut(s) 323, 1060, 1111, 1656, 1926, 1992, 2319
FbaI TGATCA 2 cut(s) 33, 187
FblI GTMKAC 3 cut(s) 1268, 1707, 2475
FokI GGATG 7 cut(s) 118, 197, 912, 1870, 2357, 2516, 2562
FspBI CTAG 4 cut(s) 116, 311, 2370, 2617
GlaI GCGC 2 cut(s) 702, 1887
HaeII RGCGCY 1 cut(s) 1889
HaeIII GGCC 5 cut(s) 424, 1092, 1252, 1261, 2327
HapII CCGG 3 cut(s) 102, 451, 1555
HgaI GACGC 1 cut(s) 2147
HhaI GCGC 2 cut(s) 703, 1888
Hin1I GRCGYC 2 cut(s) 335, 2139
Hin1II CATG 7 cut(s) 327, 1064, 1115, 1660, 1930, 1996, 2323
Hin6I GCGC 2 cut(s) 701, 1886
HinP1I GCGC 2 cut(s) 701, 1886
HindIII AAGCTT 3 cut(s) 438, 1644, 1786
HpaII CCGG 3 cut(s) 102, 451, 1555
HphI GGTGA 7 cut(s) 551, 763, 1507, 1547, 1798, 1879, 2306
Hpy166II GTNNAC 5 cut(s) 799, 1269, 1708, 2443, 2476
Hpy188III TCNNGA 9 cut(s) 31, 91, 850, 929, 1599, 1657, 1751, 2135, 2544
Hpy8I GTNNAC 5 cut(s) 799, 1269, 1708, 2443, 2476
Hpy99I CGWCG 1 cut(s) 2141
HpyAV CCTTC 6 cut(s) 50, 1017, 1469, 2309, 2529, 2595
HpyCH4III ACNGT 9 cut(s) 40, 137, 514, 938, 1350, 1429, 1748, 1936, 1957
HpyCH4IV ACGT 5 cut(s) 228, 335, 1308, 1481, 2110
HpyF3I CTNAG 9 cut(s) 6, 240, 348, 414, 1186, 1313, 2098, 2105, 2375
HpySE526I ACGT 5 cut(s) 228, 335, 1308, 1481, 2110
Hsp92I GRCGYC 2 cut(s) 335, 2139
Hsp92II CATG 7 cut(s) 327, 1064, 1115, 1660, 1930, 1996, 2323
HspAI GCGC 2 cut(s) 701, 1886
Ksp22I TGATCA 2 cut(s) 33, 187
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 3 cut(s) 827, 1108, 1694
LweI GCATC 6 cut(s) 511, 559, 967, 1513, 2335, 2603
MabI ACCWGGT 1 cut(s) 603
MaeI CTAG 4 cut(s) 116, 311, 2370, 2617
MaeII ACGT 5 cut(s) 228, 335, 1308, 1481, 2110
MaeIII GTNAC 8 cut(s) 446, 508, 938, 1145, 1637, 2260, 2281, 2294
MfeI CAATTG 2 cut(s) 375, 471
MflI RGATCY 4 cut(s) 1217, 1369, 1411, 2012
MhlI GDGCHC 3 cut(s) 1687, 2434, 2543
MlsI TGGCCA 2 cut(s) 1092, 1252
MluNI TGGCCA 2 cut(s) 1092, 1252
MlyI GAGTC 3 cut(s) 358, 687, 855
MmeI TCCRAC 3 cut(s) 1138, 1352, 2191
Mox20I TGGCCA 2 cut(s) 1092, 1252
Mph1103I ATGCAT 1 cut(s) 2596
MroXI GAANNNNTTC 1 cut(s) 1664
MscI TGGCCA 2 cut(s) 1092, 1252
MseI TTAA 9 cut(s) 638, 807, 882, 1067, 1080, 2076, 2276, 2495, 2562
MslI CAYNNNNRTG 1 cut(s) 1130
Msp20I TGGCCA 2 cut(s) 1092, 1252
MspA1I CMGCKG 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 3 cut(s) 102, 451, 1555
MspR9I CCNGG 5 cut(s) 103, 605, 951, 1254, 1555
MunI CAATTG 2 cut(s) 375, 471
Mva1269I GAATGC 1 cut(s) 2596
MvaI CCWGG 3 cut(s) 605, 951, 1254
NciI CCSGG 2 cut(s) 103, 1555
NcoI CCATGG 1 cut(s) 1992
NlaIII CATG 7 cut(s) 327, 1064, 1115, 1660, 1930, 1996, 2323
NlaIV GGNNCC 4 cut(s) 875, 1696, 2014, 2196
NmuCI GTSAC 2 cut(s) 508, 2294
NsiI ATGCAT 1 cut(s) 2596
NspI RCATGY 3 cut(s) 1115, 1930, 2323
NspV TTCGAA 4 cut(s) 723, 904, 1473, 2588
PaeI GCATGC 1 cut(s) 1930
PagI TCATGA 1 cut(s) 1656
PaqCI CACCTGC 1 cut(s) 1193
PciI ACATGT 1 cut(s) 1111
PciSI GCTCTTC 1 cut(s) 21
PctI GAATGC 1 cut(s) 2596
PdmI GAANNNNTTC 1 cut(s) 1664
PfeI GAWTC 9 cut(s) 98, 197, 208, 533, 658, 954, 1182, 1487, 1595
PfoI TCCNGGA 2 cut(s) 101, 1553
PinAI ACCGGT 1 cut(s) 450
PleI GAGTC 3 cut(s) 358, 686, 854
PpsI GAGTC 3 cut(s) 358, 686, 854
Ppu21I YACGTR 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 314
PscI ACATGT 1 cut(s) 1111
PshBI ATTAAT 2 cut(s) 638, 2562
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 3 cut(s) 603, 949, 1252
PspEI GGTNACC 2 cut(s) 2281, 2294
PspGI CCWGG 3 cut(s) 603, 949, 1252
PspN4I GGNNCC 4 cut(s) 875, 1696, 2014, 2196
PspPI GGNCC 5 cut(s) 314, 423, 1259, 1626, 2195
PspPPI RGGWCCY 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 4 cut(s) 1217, 1369, 1411, 2012
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 4 cut(s) 919, 1290, 2397, 2406
RsaNI GTAC 4 cut(s) 918, 1289, 2396, 2405
RseI CAYNNNNRTG 1 cut(s) 1130
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 9 cut(s) 638, 807, 882, 1067, 1080, 2076, 2276, 2495, 2562
Sau96I GGNCC 5 cut(s) 314, 423, 1259, 1626, 2195
SchI GAGTC 3 cut(s) 358, 687, 855
ScrFI CCNGG 5 cut(s) 103, 605, 951, 1254, 1555
SduI GDGCHC 3 cut(s) 1687, 2434, 2543
SexAI ACCWGGT 1 cut(s) 603
SfaNI GCATC 6 cut(s) 511, 559, 967, 1513, 2335, 2603
SfiI GGCCNNNNNGGCC 1 cut(s) 1258
SfuI TTCGAA 4 cut(s) 723, 904, 1473, 2588
SinI GGWCC 3 cut(s) 314, 1626, 2195
SmiMI CAYNNNNRTG 1 cut(s) 1130
SmlI CTYRAG 6 cut(s) 574, 650, 996, 1066, 2542, 2610
SmoI CTYRAG 6 cut(s) 574, 650, 996, 1066, 2542, 2610
SphI GCATGC 1 cut(s) 1930
SsiI CCGC 3 cut(s) 710, 1106, 1911
SspI AATATT 2 cut(s) 2065, 2499
SspMI CTAG 4 cut(s) 116, 311, 2370, 2617
StyD4I CCNGG 5 cut(s) 101, 603, 949, 1252, 1553
StyI CCWWGG 3 cut(s) 1262, 1992, 2161
TaaI ACNGT 9 cut(s) 40, 137, 514, 938, 1350, 1429, 1748, 1936, 1957
TaiI ACGT 5 cut(s) 231, 338, 1311, 1484, 2113
TaqI TCGA 8 cut(s) 90, 129, 723, 891, 904, 1473, 2136, 2588
TaqII GACCGA 1 cut(s) 2212
TfiI GAWTC 9 cut(s) 98, 197, 208, 533, 658, 954, 1182, 1487, 1595
Tru1I TTAA 9 cut(s) 638, 807, 882, 1067, 1080, 2076, 2276, 2495, 2562
Tru9I TTAA 9 cut(s) 638, 807, 882, 1067, 1080, 2076, 2276, 2495, 2562
TscAI CASTG 9 cut(s) 186, 208, 364, 517, 781, 996, 1402, 1939, 2196
TseFI GTSAC 2 cut(s) 508, 2294
Tsp45I GTSAC 2 cut(s) 508, 2294
TspGWI ACGGA 2 cut(s) 2051, 2279
TspRI CASTG 9 cut(s) 186, 208, 364, 517, 781, 996, 1402, 1939, 2196
Vha464I CTTAAG 1 cut(s) 1066
VpaK11BI GGWCC 3 cut(s) 314, 1626, 2195
VspI ATTAAT 2 cut(s) 638, 2562
XagI CCTNNNNNAGG 1 cut(s) 1473
XapI RAATTY 2 cut(s) 1517, 1574
XceI RCATGY 3 cut(s) 1115, 1930, 2323
XcmI CCANNNNNNNNNTGG 1 cut(s) 1260
XmiI GTMKAC 3 cut(s) 1268, 1707, 2475
XmnI GAANNNNTTC 1 cut(s) 1664
XspI CTAG 4 cut(s) 116, 311, 2370, 2617
ZraI GACGTC 1 cut(s) 336
Zsp2I ATGCAT 1 cut(s) 2596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.