pycom11g24140

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012725
Physical Location & Seq
Forward (+)
27143094 .. 27143997
904 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
ATGGCTAAAGCAGCTCTCTCTGTTCTGCTCCTGCTCTTGTCCTTCACTTCAGAAACTTTGCTCATGGTAAATGGACAGAAAACTTGGTGTGTGGCCAGGCCTTCGTCCGACCAAGCAACGCTCTTATCGAATCTAAATTACGCGTGCGCGCATGTAGATTGTCAGATTCTTCGAAAAGGATGCCCGTGCTCATCGCCGGATAATCTCATGAACCGTGCCTCCATAGCCATGAACATGTATTTCCAATCCAAGGGAAAGAACCAGTGGAATTGTGACTTCAGAGGCTCTGCTCTTATTGTCGTGACTGATCCAAGTTATGGTGACTGCATTTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

113

Amino Acids

12.28

Weight (kDa)

8.19

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 28 - 97 1.4e-18 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78520 AT2G43670
fragaria_vesca FvH4_3g17340
malus_domestica MD11G1273700.v1.1
prunus_persica Prupe.4G153400_v2.0.a1
pyrus_communis pycom11g24140
rosa_chinensis RchiOBHm_Chr5g0028861
rosa_laevigata RLG00000033120
rosa_multiflora Rmu_sc0005178.1_g000012
rosa_roxburghii Rroxscaffold_1G00051020
rosa_rugosa Rorug05G0110800 Rorug05G0110900 Rorug05G0111000
rosa_samantha Rh5AG203300 Rh5BG201400 Rh5CG222400 Rh5DG205000
rosa_wichuraiana Rw5G018390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 317
AccII CGCG 2 cut(s) 143, 149
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 2 cut(s) 33, 262
AfiI CCNNNNNNNGG 2 cut(s) 250, 317
AflIII ACRYGT 2 cut(s) 141, 234
AjnI CCWGG 1 cut(s) 95
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw21I GWGCWC 1 cut(s) 191
AlwI GGATC 1 cut(s) 302
AoxI GGCC 2 cut(s) 93, 98
ApeKI GCWGC 1 cut(s) 11
AspLEI GCGC 2 cut(s) 149, 151
AsuHPI GGTGA 1 cut(s) 332
AsuII TTCGAA 1 cut(s) 172
BalI TGGCCA 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 23
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 1 cut(s) 97
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 317
Bse1I ACTGG 1 cut(s) 262
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 2 cut(s) 250, 317
BseNI ACTGG 1 cut(s) 262
BsePI GCGCGC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 143, 149
BshFI GGCC 2 cut(s) 95, 100
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 250, 317
BsnI GGCC 2 cut(s) 95, 100
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 307
BspANI GGCC 2 cut(s) 95, 100
BspFNI CGCG 2 cut(s) 143, 149
BspHI TCATGA 1 cut(s) 207
BspPI GGATC 1 cut(s) 302
BspT104I TTCGAA 1 cut(s) 172
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 249
BssHII GCGCGC 1 cut(s) 147
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 145, 149
BstF5I GGATG 1 cut(s) 185
BstFNI CGCG 2 cut(s) 143, 149
BstHHI GCGC 2 cut(s) 149, 151
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 2 cut(s) 11, 224
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 2 cut(s) 155, 238
BstSCI CCNGG 1 cut(s) 95
BstUI CGCG 2 cut(s) 143, 149
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 95, 100
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 145, 149
CciI TCATGA 1 cut(s) 207
CfoI GCGC 2 cut(s) 149, 151
CviAII CATG 5 cut(s) 64, 152, 208, 229, 235
CviJI RGCY 6 cut(s) 5, 14, 95, 100, 227, 285
CviKI_1 RGCY 6 cut(s) 5, 14, 95, 100, 227, 285
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 33, 262
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 5 cut(s) 67, 155, 211, 232, 238
FaiI YATR 9 cut(s) 65, 153, 209, 224, 230, 236, 318, 333, 337
FatI CATG 5 cut(s) 63, 151, 207, 228, 234
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 12
GlaI GCGC 2 cut(s) 148, 150
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 2 cut(s) 95, 100
HapII CCGG 1 cut(s) 197
HhaI GCGC 2 cut(s) 149, 151
Hin1II CATG 5 cut(s) 67, 155, 211, 232, 238
Hin6I GCGC 2 cut(s) 147, 149
HinP1I GCGC 2 cut(s) 147, 149
HinfI GANTC 2 cut(s) 130, 166
HpaII CCGG 1 cut(s) 197
HphI GGTGA 1 cut(s) 332
Hpy188I TCNGA 4 cut(s) 52, 109, 165, 281
Hpy188III TCNNGA 2 cut(s) 208, 301
HpyAV CCTTC 2 cut(s) 52, 111
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 327, 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 224
Hsp92II CATG 5 cut(s) 67, 155, 211, 232, 238
HspAI GCGC 2 cut(s) 147, 149
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 5 cut(s) 44, 82, 109, 210, 275
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 272, 301, 320
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 2 cut(s) 136, 268
MluI ACGCGT 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 2 cut(s) 229, 275
Mox20I TGGCCA 1 cut(s) 95
Mph1103I ATGCAT 1 cut(s) 337
MscI TGGCCA 1 cut(s) 95
MslI CAYNNNNRTG 2 cut(s) 227, 233
Msp20I TGGCCA 1 cut(s) 95
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 2 cut(s) 143, 149
MwoI GCNNNNNNNGC 2 cut(s) 11, 224
NdeII GATC 1 cut(s) 307
NlaIII CATG 5 cut(s) 67, 155, 211, 232, 238
NmuCI GTSAC 3 cut(s) 272, 301, 320
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 2 cut(s) 155, 238
NspV TTCGAA 1 cut(s) 172
PagI TCATGA 1 cut(s) 207
PauI GCGCGC 1 cut(s) 147
PceI AGGCCT 1 cut(s) 100
PciI ACATGT 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PfeI GAWTC 2 cut(s) 130, 166
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 1 cut(s) 13
PscI ACATGT 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PteI GCGCGC 1 cut(s) 147
RseI CAYNNNNRTG 2 cut(s) 227, 233
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 191
SetI ASST 1 cut(s) 16
SfaNI GCATC 1 cut(s) 170
SfuI TTCGAA 1 cut(s) 172
SmiMI CAYNNNNRTG 2 cut(s) 227, 233
Sse9I AATT 2 cut(s) 136, 268
SseBI AGGCCT 1 cut(s) 100
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 128, 172
TasI AATT 2 cut(s) 136, 268
TfiI GAWTC 2 cut(s) 130, 166
TscAI CASTG 1 cut(s) 269
TseFI GTSAC 3 cut(s) 272, 301, 320
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 3 cut(s) 272, 301, 320
TspDTI ATGAA 2 cut(s) 224, 245
TspRI CASTG 1 cut(s) 269
Van91I CCANNNNNTGG 1 cut(s) 317
XceI RCATGY 2 cut(s) 155, 238
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.