pycom11g24450

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016003
Physical Location & Seq
Reverse (-)
27343479 .. 27355310
11832 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6132 bp
ATGTCTAAGATACAAGAATATACGAATTGGGCTTGGATTCGTTCACTCGTGGGAGAAACGCGCAATCATTTCACAACCCCATTGGCTCTTTTACCCATAAACTATAAAGTGAAGGAAACTGCTCATGACACCCTTCCAAACGAAGAAATTCATGTAGTTCCCTCTCTCAAAAGAGAAAGCAAAACACAGCTCCAAAAGCAAAAGCAAAAAGATAACAAATCCTCTTTATTGTCCCTTGTTCACATTCCCACCATTCCCGTTGAGCTCCCAGCAATGTCTGAGATTGTGCTTGCAGAGCCTATCTATGAAGGCGACCTGGAAGAGCCGCAAGGGCCCGTTACACGGGCCATTACTATGGGCTCTACAAGTTATCACAATCATGTTCCTGAACCCTTTGAGAGCGAGAGATTGCCTGCTACTCTGGCTTCTGAAATCCAGAGGTTTCTGCGTGTTGCAAATTTGATAGAAATCGAGGAGCCCCGTATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAATATGGACCGCAATTCCACTGGTCGGGGTGTTCGACAATTCAAAACTTCCCTTCTCCAGCGACTTGAACAGGATGAAGAAACTACAATAAGCAAACGGAAGGAAGAGAGTGATATACGTGAGCTGAGACGTGTCTATCATGCTCACAAAGAGTACATCATTAAACATGATAGAGCGTTTCATTTGGAGAATAGACACAGAGAGCAGTTGATAAATGCACGTAGAATCGGCTCTGTTTTGTTTGAAGTTCTAAAGAGAGTTTCAAATACAAACCCTCAGGCACTTGCTAATAGAGGTCGCATCCAGAAAAAATCTAATGATCTCTTCGTGCCATATAATATTCTACCACTCGATCATGGAGGTATTCAGCAACCAATTATGAAACTACCTGAGATTAAGGCTGCACTAGCTGCTATTCGCAATATTCGTGGTATACCTTCGGCCAATGATTTTCAGAAACATGGGGATTTCATTGATATGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAGGGAAATGTCACCAATCAGAGGGAACATCTACTTCTACTCCTTGCTAACATTCACATACGAAAAACTCATAAGCCGGCATCTGTCTCAAAGTTAGAAGATGGTGCAGTGGATGAGTTAATGCGCAAATTTTTTAAAAATTATACAAACTGGTGCAAATTTTTTGGTAGGAAAAGCAACATCCGGTTGCCTTATGTGAAACAAGAAGCACAACAGTATAAACTTTTATATCTAGCGCTTTACCTTCTTATCTGGGGGGAGGCTGCAAATCTGCGATTCATGCCAGAGTGTCTATGTTATATTTTTCACCATATGGCATATGAGTTACATGGTATGCTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAAGTCATGCCAGCATATGGAGGCCAGCCCGAGTCTTTCCTCAACAATGTAGTTACCCCCATATACACGGTTATAAGCGAGGAAGCCAAGAAAAGCAAAGGTGGGACAACTGACCATGCCATGTGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTCGAAATAGGTTGGCCCATGCGTCTAGACCATGATTTCTTCTGCACACAACCTTCAAAGAAACCAAAACCTAAGAAGGCTTCAGTATCCACTGGTTCTGTAGAAGAAAGGAGGGAAGACGGGGAGGAAGTGGAAGTGGGGGCTACTAATAAAGAAGAAGATCGCGAGCCCCAATGGTTAGGGAAAACAAATTTTGTAGAAGTCCGTTCATTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTAATTCTTTCACTTCAGGCATTGATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAACTGTTTGACAAGGTTATATTCGAGGATGTCATGAGCATATTTGTCACATCTGCGTTTCTTAAACTCTTACGAGCAATGCTCGACATTGTATTTACATGGAAAGCCAGACAAACAATGGAATTCTCTGGAAAAGTAAGACATGTTATGAAGCTGGTTGTTGCAGCGATATGGACTATAGTTCTTCCTGTTTATTATGGAAATTCTAGAAGAAAATATACTTGTTATCCTGCACGATATGGAAGCTGGCTTCAGGAGTGGTGTTTCTCCTCCTATATGGTTGCAGTTGCAATTTATTTGATGACTAACGCGGTTGAGATGGTGCTGTTTTTGGTTCCTTCTATTCGCAAATACATCGAGATCTCAAATTACCGAATATGCACTATTTTGTCTTGGTGGACACAGTCTGGATTATATATTGGTCGTGGGATGCAAGAAAGCCAGCTTTCAGTTTTGAAGTATACAGTGTTTTGGGTGCTAGTACTACTGAGCAAATTTTCTTTCAGCTACTTCTTCGAGATAAAACCGCTCATAGAACCAACGAAACAGATCATTAAACTTAGTGTGAAACATTATGAGTGGCACGAGCTTTTTCCGACAGTTCAGAGTAATGCCGGTGTAATTGTTGCTGTATGGGCTCCAATCGTAGTTGTGTATTTTATGGACACTCAGATATGGTATTCTGTTTACTGCACAATCTTTGGTGGAGTCTATGGCATATTGCACCACCTTGGTGAGATTCGGACGTTGGGGATGTTGAGAAGTCGGTTTCACACTTTGCCTTCTGCATTCAATGTTTCCCTTATCCCACCCTCATCAAGAAATGATCGAAAGAGAAAAAACGGTTTCTTTCACAATAAATTCAAAAAGGTCTCAAAGACTGAAAACAATGGCCTTGCAAAGTTTGTATTAGTTTGGAACCAAATTATCAATAGCTTTCGAACGGAGGACTTGATAAACAACAGGGAGTTGGATTTGATGAGTATGCCTATGTCATCAGAACTATTTTCTGGCAGAGTTCGTTGGCCTGTTTTCCTTCTTGCGAATAAGTTTTCAACAGCGCTGAGTATCGCAAAGGATTTTGTCGGGAAGGATGAGATTCTTATCAGAAAAATCAAAAAAGACGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCCTTGAAGTACATCCTCGAAATCCTCATTGTTGGTGATCTTGAAAAAAGTATTGTATCTGCCATGCTGACTGAAATTGAAGAAAGCATCGCAAGGTCAAGTCTTCTTGAGGATTTAAGGATGACTGAACTTCCACATCTTCTCGCCAAATGTATCGAGTTGATTGAACTTCTGGTTGAGGGAAACGAAGAGCATCACAGTAAAGTTGTGAAGATTCTTCAAGATATATTTGAGCTTGTAACCAACGATATGATGACTAATGGCTCCAGGATACTGGATTTGCTGAATTCATTCCAACAAATCGACGTGGACTTTGTTGATTTTAAGAGAACCATTGAACCAGAATTATTTGGCTCCGCTGATGACAAGAACTCCATCCATTTCCCTTTGCCAGACGACAGTGCTTCTCTGATTGAGCAGATCAAGCGTTTCCATCTGTTGCTTACTGTCAAGGATACAGCATTGGACATTCCAACTAACTTGGAGGCTCGAAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAACATGCCCGGCGCACCCAAAGTCTGCAATATGCTGCCTTTCTGTGTTATGACACCACACTTCATGGAGGATATTAATTTTTCAATGAAGGAGCTTCATTCGAGCCAACGAGAGGTCTCCATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCTTGGAGCGCATGGGTTGTGAAAACTTGGATGGATTGAAAGAGGAAAGCAAGGAGGAAGAGCTTAGGAATTGGGCTTCCTACCGGGGCCAAACGCTAAGCAGAACAGTTCGAGGAATGATGTACTATAGAGAAGCCTTAAAATTACAAGCATTCCTCGACGTGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCCGTTGAAAGTAGAAATCACCTGTTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTACTTACTTGTCAATTGTTTGGATCACAAAAAGCTTCTGGAGATCCACGTGCAAAAGACATTATTGACTTGATGAAAAGACACCCATCTCTCCGCGTTGCTTACGTTGAGGAGAAAGAAGTGATAGGTGGAAAAAAGCCTCAGAAAGTTTATTCTTCTGTATTGGTTAAAGCTGTCAATGATTTTGATCAGGAAATATATCGCATAAAGCTTCCTGGTCCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAATCACGGGATAATTTTCACTCGTGGTGAAGCTCTCCAGACCATTGATATGAACCAAGATAGTTATCTGGAAGAAGCATTCAAAATGAGAAACGTTCTGCAAGAATTTCTTCAGAACCAAGGACGGAGACCTCCTACGCTACTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTTGCTTGGTTCATGTCGTATCAAGAGACAAGCTTCGTCACCATTGGTCAAAGGCTTCTTGCTAATCCCCTCAAGGTACGGTTCCATTATGGACATCCCGATGTATTTGACAGACTATTTCACATCACAAGAGGCGGCATAAGCAAAGCATCAAAAACAATTAACCTAAGCGAGGATGTTTTTGCTGGATTTAACTGCACTTTACGACGTGGATGTATAACTTATCACGAGTACATGCAAGTTGGCAAGGGGCGTGATGTAAGTCTGAACCAGATATCAAAGTTCGAAGCTAAGGTTGCTAACGGAAACAGTGAACAGACTATAAGCCGTGACATATACCACCTTGGACGCCAGTTTGATTTCTTCCGGATGCTATCATGTTATTTCACAACCATTGGGTTTTACTTTAGTAGCCTGATGTCAGTTATTGGAATATACGTCTTCCTTTACGGACAGTTATACCTTGTCCTCAGTGGCTTGGAAAGAGTGATTATAGTTGAAGCAAGACTGCAGAACATACAATCCTTGGAAACGGCTCTTGCATCGCAGTCATTTATACAGCTAGGTCTACTAACAGGATTACCAATGGTGATGGAAATTGGGCTAGAGAAGGGATTCCTTAACGCGCTTAAAGATTTTGTCCTGATGCAGTTGCAGCTGGCTTCTATTTTCTTCACCTTCTCCTTCGGCACAAAAATACATTATTATGGCCGAGCAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTGGTTGTGTTCCATACTAGCTTCACTGAGAACTACAGGTCATATTCAAGAAGCCACTTTGTGAAAGGATTCGAGTTGTTGCTCCTGTTGATTGTTTACGATTTATATAGGCGGTCTTATCAGAGCAGCATGGCGTATGTATTAATCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTTGCTCCATTTCTTTTTAATCCCTCCGGATTCAGTTGGGAGAAGATAGTAGACGACTGGAAAGATTGGAACAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCAACAAGACAAAAGTTGGCAGTCTTGGTGGATGGATGAGCAAGACCATCTTCGCCGTTCAGGGATGACTTCTAGGTTGCTTGAAATACTCCTTTCCGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATCTCCCAGAATAGCATGAATTTTCTGGTATATTTGCTTTCATGGATTGTGATCCTCGCAGTTTTCTTGTTAGTAAAGGCAGTGAATCTCGGAAGGAAACAGTTCAGCGCTAACTATCACCTCGTGTTTAGGCTGTTCAAGGCATTCCTCTTCTTGGGTGTTCTTGCTGTCATACTTGCTCTCTATTTTATATGCAAACTCTCCTGGAAGGACTTAGTCGTCTGCTGTCTAGCATTTTTTCCGACAGGCTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTCTGGGAATTCACCAGAGTACTCGCCAAGACTTATGACTACGGAATGGGTGTTGCTCTCTTTGCACCTATCGCGGTCTTTTCATGGCTTCCGATTATATCAGCATTCCAGACTCGTTTCCTGTTCAACGAGGCGTTCAACAGGCACTTACAGATCCAACCAATTCTTGCAGGGAAGAAGAAGAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

2044

Amino Acids

236.93

Weight (kDa)

8.68

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 145 - 268 1.4e-08 Vta1 like
FKS1_dom1 PF14288 420 - 531 1.7e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 973 - 1167 1.4e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1174 - 1263 5.8e-24 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1260 - 1863 6.1e-195 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1481
AasI GACNNNNNNGTC 1 cut(s) 5855
Acc16I TGCGCA 1 cut(s) 1163
AccB7I CCANNNNNTGG 3 cut(s) 1424, 1876, 4241
AccBSI CCGCTC 1 cut(s) 2419
AccI GTMKAC 5 cut(s) 958, 2351, 5017, 5442, 5625
AccII CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
AccIII TCCGGA 2 cut(s) 4815, 5417
AclI AACGTT 1 cut(s) 4359
AclWI GGATC 5 cut(s) 4042, 4049, 5477, 5683, 6088
AcoI YGGCCR 2 cut(s) 966, 5158
AcuI CTGAAG 6 cut(s) 1001, 1644, 1829, 2126, 3933, 4361
AcvI CACGTG 1 cut(s) 4061
AcyI GRCGYC 1 cut(s) 4798
AdeI CACNNNGTG 2 cut(s) 5269, 5761
AfeI AGCGCT 3 cut(s) 1275, 2952, 5746
AflIII ACRYGT 3 cut(s) 655, 2032, 3021
AjiI CACGTC 4 cut(s) 656, 3358, 3907, 4658
AjnI CCWGG 4 cut(s) 315, 3317, 4225, 5840
AjuI GAANNNNNNNTTGG 2 cut(s) 3731, 3763
AleI CACNNNNGTG 1 cut(s) 2622
AloI GAACNNNNNNTCC 2 cut(s) 4955, 4987
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 7 cut(s) 646, 1129, 1572, 2767, 3700, 4387, 4469
AlwI GGATC 5 cut(s) 4042, 4049, 5477, 5683, 6088
Ama87I CYCGRG 1 cut(s) 1436
Aor13HI TCCGGA 2 cut(s) 4815, 5417
Aor51HI AGCGCT 3 cut(s) 1275, 2952, 5746
ApaI GGGCCC 1 cut(s) 336
ApeKI GCWGC 8 cut(s) 926, 935, 1301, 2054, 3613, 5104, 5334, 5476
ArsI GACNNNNNNTTYG 2 cut(s) 3347, 3379
AseI ATTAAT 2 cut(s) 3654, 5351
Asp700I GAANNNNTTC 9 cut(s) 147, 3341, 3743, 3924, 4374, 4441, 5063, 5276, 5738
AspLEI GCGC 8 cut(s) 63, 1164, 1276, 2953, 3593, 3756, 5077, 5747
AspS9I GGNCC 8 cut(s) 332, 333, 345, 532, 1593, 3832, 4229, 5919
AsuC2I CCSGG 2 cut(s) 3588, 3830
AsuII TTCGAA 3 cut(s) 1581, 2830, 4734
AvaI CYCGRG 1 cut(s) 1436
AvaII GGWCC 2 cut(s) 532, 4229
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 1 cut(s) 336
BanII GRGCYC 6 cut(s) 267, 336, 362, 480, 1749, 2530
BarI GAAGNNNNNNTAC 4 cut(s) 2092, 2124, 3029, 3061
BauI CACGAG 5 cut(s) 47, 2474, 4287, 4676, 5759
BbrPI CACGTG 1 cut(s) 4061
BbsI GAAGAC 4 cut(s) 1701, 3146, 3550, 4882
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 8 cut(s) 913, 922, 1288, 2066, 3600, 5116, 5346, 5488
BceAI ACGGC 4 cut(s) 3929, 4761, 4998, 5535
BcgI CGANNNNNNTGC 2 cut(s) 2227, 2261
BciT130I CCWGG 4 cut(s) 317, 3319, 4227, 5842
BciVI GTATCC 3 cut(s) 1675, 3315, 3499
BclI TGATCA 1 cut(s) 4198
BcnI CCSGG 2 cut(s) 3588, 3830
BcoDI GTCTC 7 cut(s) 646, 1129, 1572, 2767, 3700, 4387, 4469
BfmI CTRYAG 5 cut(s) 1677, 2067, 3871, 4958, 5242
BfoI RGCGCY 3 cut(s) 1277, 2954, 5748
BfuI GTATCC 3 cut(s) 1675, 3315, 3499
BglI GCCNNNNNGGC 1 cut(s) 331
BglII AGATCT 1 cut(s) 2250
BlpI GCTNAGC 2 cut(s) 3842, 3975
BmcAI AGTACT 3 cut(s) 1022, 2373, 5961
Bme1390I CCNGG 6 cut(s) 317, 3319, 3588, 3830, 4227, 5842
Bme18I GGWCC 2 cut(s) 532, 4229
BmeT110I CYCGRG 1 cut(s) 1436
BmgBI CACGTC 4 cut(s) 656, 3358, 3907, 4658
BmgT120I GGNCC 8 cut(s) 332, 333, 345, 532, 1593, 3832, 4229, 5919
BmrFI CCNGG 6 cut(s) 317, 3319, 3588, 3830, 4227, 5842
BmrI ACTGGG 1 cut(s) 5208
BmuI ACTGGG 1 cut(s) 5208
BpiI GAAGAC 4 cut(s) 1701, 3146, 3550, 4882
BplI GAGNNNNNCTC 2 cut(s) 1956, 1988
BpmI CTGGAG 4 cut(s) 566, 3301, 4071, 4286
Bpu10I CCTNAGC 3 cut(s) 3809, 4616, 4740
Bpu1102I GCTNAGC 2 cut(s) 3842, 3975
Bpu14I TTCGAA 3 cut(s) 1581, 2830, 4734
BpuEI CTTGAG 2 cut(s) 3179, 4505
BpuMI CCSGG 2 cut(s) 3588, 3830
BsaAI YACGTR 3 cut(s) 644, 746, 4061
BsaBI GATNNNNATC 5 cut(s) 467, 2993, 4197, 4329, 5687
BsaHI GRCGYC 1 cut(s) 4798
BsaI GGTCTC 4 cut(s) 1572, 2767, 3700, 4387
BsaJI CCNNGG 5 cut(s) 2620, 3829, 4384, 4792, 4974
BsaWI WCCGGW 3 cut(s) 1221, 4815, 5417
BsaXI ACNNNNNCTCC 4 cut(s) 1062, 1092, 3407, 3437
Bse118I RCCGGY 2 cut(s) 1114, 2504
Bse1I ACTGG 9 cut(s) 550, 1193, 1384, 1675, 3330, 4801, 5203, 5453, 5941
Bse21I CCTNAGG 1 cut(s) 801
Bse3DI GCAATG 2 cut(s) 279, 1974
Bse8I GATNNNNATC 5 cut(s) 467, 2993, 4197, 4329, 5687
BseAI TCCGGA 2 cut(s) 4815, 5417
BseBI CCWGG 4 cut(s) 317, 3319, 4227, 5842
BseDI CCNNGG 5 cut(s) 2620, 3829, 4384, 4792, 4974
BseJI GATNNNNATC 5 cut(s) 467, 2993, 4197, 4329, 5687
BseMI GCAATG 2 cut(s) 279, 1974
BseNI ACTGG 9 cut(s) 550, 1193, 1384, 1675, 3330, 4801, 5203, 5453, 5941
BseRI GAGGAG 3 cut(s) 488, 2149, 4136
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 8 cut(s) 913, 922, 1288, 2066, 3600, 5116, 5346, 5488
BseYI CCCAGC 2 cut(s) 268, 5886
BsgI GTGCAG 6 cut(s) 912, 1164, 1606, 2106, 2566, 4630
Bsh1236I CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 7 cut(s) 1115, 1222, 2505, 3588, 3829, 4816, 5418
BslFI GGGAC 2 cut(s) 217, 1525
BsmAI GTCTC 7 cut(s) 646, 1129, 1572, 2767, 3700, 4387, 4469
BsmBI CGTCTC 1 cut(s) 646
BsmFI GGGAC 2 cut(s) 217, 1525
BsmI GAATGC 5 cut(s) 2678, 3896, 4343, 5780, 6044
Bso31I GGTCTC 4 cut(s) 1572, 2767, 3700, 4387
BsoBI CYCGRG 1 cut(s) 1436
Bsp119I TTCGAA 3 cut(s) 1581, 2830, 4734
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 6 cut(s) 267, 336, 362, 480, 1749, 2530
Bsp13I TCCGGA 2 cut(s) 4815, 5417
Bsp1720I GCTNAGC 2 cut(s) 3842, 3975
Bsp68I TCGCGA 1 cut(s) 1743
BspEI TCCGGA 2 cut(s) 4815, 5417
BspFNI CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
BspHI TCATGA 2 cut(s) 124, 1923
BspMAI CTGCAG 1 cut(s) 4962
BspPI GGATC 5 cut(s) 4042, 4049, 5477, 5683, 6088
BspQI GCTCTTC 3 cut(s) 315, 3234, 3798
BspT104I TTCGAA 3 cut(s) 1581, 2830, 4734
BspTNI GGTCTC 4 cut(s) 1572, 2767, 3700, 4387
BsrBI CCGCTC 1 cut(s) 2419
BsrDI GCAATG 2 cut(s) 279, 1974
BsrFI RCCGGY 2 cut(s) 1114, 2504
BsrI ACTGG 9 cut(s) 550, 1193, 1384, 1675, 3330, 4801, 5203, 5453, 5941
BssAI RCCGGY 2 cut(s) 1114, 2504
BssECI CCNNGG 5 cut(s) 2620, 3829, 4384, 4792, 4974
BssNAI GTATAC 2 cut(s) 959, 2352
BssNI GRCGYC 1 cut(s) 4798
BssSI CACGAG 5 cut(s) 47, 2474, 4287, 4676, 5759
BssT1I CCWWGG 4 cut(s) 2620, 4384, 4792, 4974
Bst1107I GTATAC 2 cut(s) 959, 2352
Bst2BI CACGAG 5 cut(s) 47, 2474, 4287, 4676, 5759
Bst2UI CCWGG 4 cut(s) 317, 3319, 4227, 5842
Bst6I CTCTTC 6 cut(s) 315, 624, 854, 3234, 3798, 5792
BstACI GRCGYC 1 cut(s) 4798
BstAPI GCANNNNNTGC 1 cut(s) 935
BstBAI YACGTR 3 cut(s) 644, 746, 4061
BstBI TTCGAA 3 cut(s) 1581, 2830, 4734
BstENI CCTNNNNNAGG 1 cut(s) 4619
BstFNI CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
BstH2I RGCGCY 3 cut(s) 1277, 2954, 5748
BstHHI GCGC 8 cut(s) 63, 1164, 1276, 2953, 3593, 3756, 5077, 5747
BstMAI GTCTC 7 cut(s) 646, 1129, 1572, 2767, 3700, 4387, 4469
BstNI CCWGG 4 cut(s) 317, 3319, 4227, 5842
BstNSI RCATGY 6 cut(s) 1869, 2036, 3025, 3586, 3715, 4687
BstSCI CCNGG 6 cut(s) 315, 3317, 3586, 3828, 4225, 5840
BstSFI CTRYAG 5 cut(s) 1677, 2067, 3871, 4958, 5242
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
BstV1I GCAGC 8 cut(s) 913, 922, 1288, 2066, 3600, 5116, 5346, 5488
BstV2I GAAGAC 4 cut(s) 1701, 3146, 3550, 4882
BstX2I RGATCY 3 cut(s) 2250, 4054, 6093
BstXI CCANNNNNNTGG 3 cut(s) 355, 3325, 3735
BstYI RGATCY 3 cut(s) 2250, 4054, 6093
BstZ17I GTATAC 2 cut(s) 959, 2352
Bsu36I CCTNAGG 1 cut(s) 801
BsuI GTATCC 3 cut(s) 1675, 3315, 3499
BtgZI GCGATG 2 cut(s) 3124, 4977
BtrI CACGTC 4 cut(s) 656, 3358, 3907, 4658
BtsI GCAGTG 2 cut(s) 1152, 5724
BtuMI TCGCGA 1 cut(s) 1743
CciI TCATGA 2 cut(s) 124, 1923
CfoI GCGC 8 cut(s) 63, 1164, 1276, 2953, 3593, 3756, 5077, 5747
Cfr10I RCCGGY 2 cut(s) 1114, 2504
Cfr13I GGNCC 8 cut(s) 332, 333, 345, 532, 1593, 3832, 4229, 5919
CseI GACGC 3 cut(s) 1589, 3555, 4806
CspCI CAANNNNNGTGG 2 cut(s) 934, 969
DraI TTTAAA 1 cut(s) 1174
DraIII CACNNNGTG 2 cut(s) 5269, 5761
DrdI GACNNNNNNGTC 1 cut(s) 5855
DseDI GACNNNNNNGTC 1 cut(s) 5855
EaeI YGGCCR 2 cut(s) 966, 5158
Eam1104I CTCTTC 6 cut(s) 315, 624, 854, 3234, 3798, 5792
EarI CTCTTC 6 cut(s) 315, 624, 854, 3234, 3798, 5792
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 4 cut(s) 2620, 4384, 4792, 4974
Eco24I GRGCYC 6 cut(s) 267, 336, 362, 480, 1749, 2530
Eco31I GGTCTC 4 cut(s) 1572, 2767, 3700, 4387
Eco32I GATATC 1 cut(s) 4725
Eco47I GGWCC 2 cut(s) 532, 4229
Eco47III AGCGCT 3 cut(s) 1275, 2952, 5746
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 6 cut(s) 1001, 1644, 1829, 2126, 3933, 4361
Eco72I CACGTG 1 cut(s) 4061
Eco81I CCTNAGG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 1436
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 4619
EcoO109I RGGNCCY 2 cut(s) 332, 5919
EcoRI GAATTC 3 cut(s) 2012, 3337, 5948
EcoRII CCWGG 4 cut(s) 315, 3317, 4225, 5840
EcoRV GATATC 1 cut(s) 4725
EcoT14I CCWWGG 4 cut(s) 2620, 4384, 4792, 4974
EcoT22I ATGCAT 1 cut(s) 3988
EcoT38I GRGCYC 6 cut(s) 267, 336, 362, 480, 1749, 2530
ErhI CCWWGG 4 cut(s) 2620, 4384, 4792, 4974
Esp3I CGTCTC 1 cut(s) 646
FalI AAGNNNNNCTT 8 cut(s) 3912, 3944, 4359, 4391, 4429, 4461, 5529, 5561
FaqI GGGAC 2 cut(s) 217, 1525
FauNDI CATATG 4 cut(s) 1350, 1357, 1423, 4009
FbaI TGATCA 1 cut(s) 4198
FblI GTMKAC 5 cut(s) 958, 2351, 5017, 5442, 5625
FriOI GRGCYC 6 cut(s) 267, 336, 362, 480, 1749, 2530
FspI TGCGCA 1 cut(s) 1163
GlaI GCGC 8 cut(s) 62, 1163, 1275, 2952, 3592, 3755, 5076, 5746
GsaI CCCAGC 2 cut(s) 272, 5890
GsuI CTGGAG 4 cut(s) 566, 3301, 4071, 4286
HaeII RGCGCY 3 cut(s) 1277, 2954, 5748
HapII CCGG 7 cut(s) 1115, 1222, 2505, 3588, 3829, 4816, 5418
HgaI GACGC 3 cut(s) 1589, 3555, 4806
HhaI GCGC 8 cut(s) 63, 1164, 1276, 2953, 3593, 3756, 5077, 5747
Hin1I GRCGYC 1 cut(s) 4798
Hin6I GCGC 8 cut(s) 61, 1162, 1274, 2951, 3591, 3754, 5075, 5745
HinP1I GCGC 8 cut(s) 61, 1162, 1274, 2951, 3591, 3754, 5075, 5745
HindIII AAGCTT 4 cut(s) 1807, 4044, 4220, 4480
HpaII CCGG 7 cut(s) 1115, 1222, 2505, 3588, 3829, 4816, 5418
Hpy99I CGWCG 3 cut(s) 3359, 3908, 4659
Hsp92I GRCGYC 1 cut(s) 4798
HspAI GCGC 8 cut(s) 61, 1162, 1274, 2951, 3591, 3754, 5075, 5745
Kpn2I TCCGGA 2 cut(s) 4815, 5417
KroI GCCGGC 1 cut(s) 1114
KroNI GCCGGC 1 cut(s) 1116
Ksp22I TGATCA 1 cut(s) 4198
LguI GCTCTTC 3 cut(s) 315, 3234, 3798
Lsp1109I GCAGC 8 cut(s) 913, 922, 1288, 2066, 3600, 5116, 5346, 5488
MaeIII GTNAC 8 cut(s) 337, 1048, 1362, 1459, 1936, 3289, 4486, 4778
MbiI CCGCTC 1 cut(s) 2419
MfeI CAATTG 1 cut(s) 4025
MflI RGATCY 3 cut(s) 2250, 4054, 6093
MhlI GDGCHC 6 cut(s) 267, 336, 362, 480, 1749, 2530
MlyI GAGTC 3 cut(s) 1448, 2607, 6046
MmeI TCCRAC 6 cut(s) 2510, 2841, 3370, 3548, 5903, 6121
Mph1103I ATGCAT 1 cut(s) 3988
MroI TCCGGA 2 cut(s) 4815, 5417
MroNI GCCGGC 1 cut(s) 1114
MroXI GAANNNNTTC 9 cut(s) 147, 3341, 3743, 3924, 4374, 4441, 5063, 5276, 5738
MslI CAYNNNNRTG 8 cut(s) 353, 378, 1001, 2622, 4313, 4548, 5154, 5178
MspA1I CMGCKG 2 cut(s) 3410, 5107
MspI CCGG 7 cut(s) 1115, 1222, 2505, 3588, 3829, 4816, 5418
MspR9I CCNGG 6 cut(s) 317, 3319, 3588, 3830, 4227, 5842
MunI CAATTG 1 cut(s) 4025
Mva1269I GAATGC 5 cut(s) 2678, 3896, 4343, 5780, 6044
MvaI CCWGG 4 cut(s) 317, 3319, 4227, 5842
MvnI CGCG 6 cut(s) 61, 1743, 2201, 4107, 5075, 6014
NaeI GCCGGC 1 cut(s) 1116
NciI CCSGG 2 cut(s) 3588, 3830
NdeI CATATG 4 cut(s) 1350, 1357, 1423, 4009
NgoMIV GCCGGC 1 cut(s) 1114
NmeAIII GCCGAG 1 cut(s) 5186
NmuCI GTSAC 4 cut(s) 1048, 1936, 4486, 4778
NruI TCGCGA 1 cut(s) 1743
NsbI TGCGCA 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 3988
NspI RCATGY 6 cut(s) 1869, 2036, 3025, 3586, 3715, 4687
NspV TTCGAA 3 cut(s) 1581, 2830, 4734
OliI CACNNNNGTG 1 cut(s) 2622
PaeI GCATGC 1 cut(s) 1869
PagI TCATGA 2 cut(s) 124, 1923
PciI ACATGT 2 cut(s) 2032, 3021
PciSI GCTCTTC 3 cut(s) 315, 3234, 3798
PcsI WCGNNNNNNNCGW 2 cut(s) 556, 949
PctI GAATGC 5 cut(s) 2678, 3896, 4343, 5780, 6044
PdiI GCCGGC 1 cut(s) 1116
PdmI GAANNNNTTC 9 cut(s) 147, 3341, 3743, 3924, 4374, 4441, 5063, 5276, 5738
PflFI GACNNNGTC 2 cut(s) 2293, 5852
PflMI CCANNNNNTGG 3 cut(s) 1424, 1876, 4241
PfoI TCCNGGA 2 cut(s) 3317, 5840
PleI GAGTC 3 cut(s) 1447, 2606, 6046
PmaCI CACGTG 1 cut(s) 4061
PmlI CACGTG 1 cut(s) 4061
PpsI GAGTC 3 cut(s) 1447, 2606, 6046
Ppu21I YACGTR 3 cut(s) 644, 746, 4061
PscI ACATGT 2 cut(s) 2032, 3021
PshBI ATTAAT 2 cut(s) 3654, 5351
PsiI TTATAA 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 267
Psp1406I AACGTT 1 cut(s) 4359
Psp6I CCWGG 4 cut(s) 315, 3317, 4225, 5840
PspCI CACGTG 1 cut(s) 4061
PspFI CCCAGC 2 cut(s) 268, 5886
PspGI CCWGG 4 cut(s) 315, 3317, 4225, 5840
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 8 cut(s) 332, 333, 345, 532, 1593, 3832, 4229, 5919
PstI CTGCAG 1 cut(s) 4962
PsuI RGATCY 3 cut(s) 2250, 4054, 6093
PsyI GACNNNGTC 2 cut(s) 2293, 5852
PvuII CAGCTG 1 cut(s) 5107
RruI TCGCGA 1 cut(s) 1743
RseI CAYNNNNRTG 8 cut(s) 353, 378, 1001, 2622, 4313, 4548, 5154, 5178
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 3 cut(s) 315, 3234, 3798
Sau96I GGNCC 8 cut(s) 332, 333, 345, 532, 1593, 3832, 4229, 5919
ScaI AGTACT 3 cut(s) 1022, 2373, 5961
SchI GAGTC 3 cut(s) 1448, 2607, 6046
ScrFI CCNGG 6 cut(s) 317, 3319, 3588, 3830, 4227, 5842
SduI GDGCHC 6 cut(s) 267, 336, 362, 480, 1749, 2530
SfcI CTRYAG 5 cut(s) 1677, 2067, 3871, 4958, 5242
SfuI TTCGAA 3 cut(s) 1581, 2830, 4734
SinI GGWCC 2 cut(s) 532, 4229
SmiMI CAYNNNNRTG 8 cut(s) 353, 378, 1001, 2622, 4313, 4548, 5154, 5178
SmlI CTYRAG 2 cut(s) 3158, 4520
SmoI CTYRAG 2 cut(s) 3158, 4520
SphI GCATGC 1 cut(s) 1869
SspI AATATT 2 cut(s) 865, 949
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 6 cut(s) 315, 3317, 3586, 3828, 4225, 5840
StyI CCWWGG 4 cut(s) 2620, 4384, 4792, 4974
TauI GCSGC 3 cut(s) 328, 501, 4587
TseFI GTSAC 4 cut(s) 1048, 1936, 4486, 4778
TseI GCWGC 8 cut(s) 926, 935, 1301, 2054, 3613, 5104, 5334, 5476
Tsp45I GTSAC 4 cut(s) 1048, 1936, 4486, 4778
TspGWI ACGGA 8 cut(s) 637, 1772, 2849, 4405, 4767, 4914, 5581, 5997
Tth111I GACNNNGTC 2 cut(s) 2293, 5852
Van91I CCANNNNNTGG 3 cut(s) 1424, 1876, 4241
VpaK11BI GGWCC 2 cut(s) 532, 4229
VspI ATTAAT 2 cut(s) 3654, 5351
XagI CCTNNNNNAGG 1 cut(s) 4619
XbaI TCTAGA 2 cut(s) 1603, 2096
XceI RCATGY 6 cut(s) 1869, 2036, 3025, 3586, 3715, 4687
XcmI CCANNNNNNNNNTGG 1 cut(s) 2005
XmiI GTMKAC 5 cut(s) 958, 2351, 5017, 5442, 5625
XmnI GAANNNNTTC 9 cut(s) 147, 3341, 3743, 3924, 4374, 4441, 5063, 5276, 5738
ZrmI AGTACT 3 cut(s) 1022, 2373, 5961
Zsp2I ATGCAT 1 cut(s) 3988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.