pycom11g26560

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011694
Physical Location & Seq
Forward (+)
29088357 .. 29090098
1742 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
ATGCAGCAAAAGTCACAGTACAAAGCTCGATCATCAATCAAAAGGTTCTCCTCTCTCCTCACGGGCAGAGTTTGGATTGAAATCTGGAACTGTATGTTGTTTGTCAATGAATGTCACTGTGCCGGACTGCTCCCCACTTCCCTTATTCTCCACTTCGTCACCCTCTCCTCTCTCTCTCTCCTCTCTCTCTCATCAATGGCAAAACCGGCTGAAGAAGCTCCTGGAACCTTAAAATACCAGACATGGGTCTTGAAAGTCTCAATCCACTGTGAAGGATGCAAGAGGAAAGTCAAGAAAGTTCTTCAGAACATCGATGGGGTTTACACGACGGATATTGATTCGCAGCAGCACAAAGTGACTGTTACCGGCAACGTGGAGGCGGAGACTCTGCTGAAGAAGCTGCAGAAGTCAGGGAAGCACGCCGAGCTTTGGCCGAAAGTTAAGAAATCGAAAAAGTCCGGGAAATCAAAGAATAGTAGTAATAACAGTGAGAAGCAGTCCGCAGATCATAAGCATGAACAAGATGGTGATCATGATCAACCGGATAAAACCAACGGCGGTGATGATGCTGACGACGGCGGTGAATCCGACGCAGAGGATGGCGAAGGTAACGAAGGTGGTGGAGAAAGCGGTGCTAGTCCTACTGTTGGTGGTGGCGGCGGCGGAAACACAGCAAGTACTGGGGCCAAAAAGAAGAAGAAAAAGAAGAAGAAGAAAGGGCAAAGTGGTAATTCCGCAAATGGCGGCGGTGTCCCTGCCAACCCCGGCGAGCACAATTTTGGAGATGCTCCGCCTCCTGCGGGCAGTATTGGGCCGTATATGGCTGGTGGGCCAGACATGGCCCAACCGATTGCCTCCATGAATCTTGACCCACCAGTTCAGCATGCGTATCTTGGCCCGTATCCAACATCAATGTACTATCAGCCCCCAACGTACGGGCTAAGTTACAATACGGCGTACCCTACCAGTACAAGTACCTCATACTATGCTCCTGATCCCATGCATACTGCTACGTATTCTCATCCGAGCTTGTTCTCGTTTGCTCTGCCGTCCGATTTTATCAATGACGATGATGAAGATACGTTCGTTGACGATGAAGATGGAGTTGGGTGCTACATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

375

Amino Acids

40.03

Weight (kDa)

6.18

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 84 - 140 5.5e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 989
AcoI YGGCCR 1 cut(s) 431
AcuI CTGAAG 3 cut(s) 231, 287, 413
AdeI CACNNNGTG 1 cut(s) 355
AfaI GTAC 7 cut(s) 20, 679, 917, 935, 959, 970, 976
AfiI CCNNNNNNNGG 5 cut(s) 244, 429, 647, 800, 935
AgsI TTSAA 2 cut(s) 80, 253
AjnI CCWGG 1 cut(s) 220
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
AluBI AGCT 5 cut(s) 26, 218, 400, 427, 1029
AluI AGCT 5 cut(s) 26, 218, 400, 427, 1029
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 2 cut(s) 262, 377
AlwI GGATC 1 cut(s) 989
AoxI GGCC 6 cut(s) 431, 684, 812, 830, 840, 895
ApeKI GCWGC 4 cut(s) 4, 343, 346, 400
AspS9I GGNCC 5 cut(s) 684, 812, 830, 841, 896
AsuC2I CCSGG 2 cut(s) 460, 765
AsuHPI GGTGA 4 cut(s) 151, 539, 572, 593
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 4 cut(s) 16, 355, 358, 387
BccI CCATC 4 cut(s) 308, 518, 593, 1094
BceAI ACGGC 5 cut(s) 571, 592, 799, 969, 1033
BciT130I CCWGG 1 cut(s) 222
BciVI GTATCC 1 cut(s) 912
BclI TGATCA 2 cut(s) 529, 535
BcnI CCSGG 2 cut(s) 460, 765
BcoDI GTCTC 2 cut(s) 262, 377
BfaI CTAG 1 cut(s) 636
BfmI CTRYAG 1 cut(s) 401
BfuI GTATCC 1 cut(s) 912
BisI GCNGC 7 cut(s) 5, 344, 347, 401, 658, 661, 745
BlsI GCNGC 7 cut(s) 6, 345, 348, 402, 659, 662, 746
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 222, 460, 765
BmgT120I GGNCC 5 cut(s) 684, 812, 830, 841, 896
BmiI GGNNCC 2 cut(s) 226, 685
BmrFI CCNGG 3 cut(s) 222, 460, 765
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 3 cut(s) 266, 556, 775
BmuI ACTGGG 1 cut(s) 690
BoxI GACNNNNGTC 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 460, 765
Bsa29I ATCGAT 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 1014
BsaBI GATNNNNATC 3 cut(s) 80, 528, 534
BsaJI CCNNGG 1 cut(s) 763
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 429, 647, 800, 935
Bse118I RCCGGY 2 cut(s) 205, 365
Bse1I ACTGG 3 cut(s) 685, 875, 966
Bse8I GATNNNNATC 3 cut(s) 80, 528, 534
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 763
BseGI GGATG 3 cut(s) 281, 604, 1021
BseJI GATNNNNATC 3 cut(s) 80, 528, 534
BseLI CCNNNNNNNGG 5 cut(s) 244, 429, 647, 800, 935
BseNI ACTGG 3 cut(s) 685, 875, 966
BseRI GAGGAG 4 cut(s) 40, 47, 157, 170
BseXI GCAGC 4 cut(s) 16, 355, 358, 387
BshFI GGCC 6 cut(s) 433, 686, 814, 832, 842, 897
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 6 cut(s) 123, 206, 366, 459, 542, 765
BsiWI CGTACG 1 cut(s) 933
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 5 cut(s) 244, 429, 647, 800, 935
BsmAI GTCTC 2 cut(s) 262, 377
BsmFI GGGAC 1 cut(s) 737
BsnI GGCC 6 cut(s) 433, 686, 814, 832, 842, 897
Bsp1286I GDGCHC 1 cut(s) 774
Bsp143I GATC 5 cut(s) 29, 505, 529, 535, 994
BspANI GGCC 6 cut(s) 433, 686, 814, 832, 842, 897
BspDI ATCGAT 1 cut(s) 312
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 226, 685
BspMAI CTGCAG 1 cut(s) 405
BspPI GGATC 1 cut(s) 989
BsrFI RCCGGY 2 cut(s) 205, 365
BsrI ACTGG 3 cut(s) 685, 875, 966
BssAI RCCGGY 2 cut(s) 205, 365
BssECI CCNNGG 1 cut(s) 763
BssMI GATC 5 cut(s) 29, 505, 529, 535, 994
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 7 cut(s) 18, 92, 119, 269, 361, 488, 646
BstBAI YACGTR 1 cut(s) 1014
BstC8I GCNNGC 4 cut(s) 420, 770, 802, 885
BstDEI CTNAG 1 cut(s) 941
BstF5I GGATG 3 cut(s) 281, 604, 1021
BstKTI GATC 5 cut(s) 32, 508, 532, 538, 997
BstMAI GTCTC 2 cut(s) 262, 377
BstMBI GATC 5 cut(s) 29, 505, 529, 535, 994
BstMWI GCNNNNNNNGC 4 cut(s) 206, 215, 397, 424
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 887
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 3 cut(s) 220, 458, 763
BstSFI CTRYAG 1 cut(s) 401
BstSNI TACGTA 1 cut(s) 1014
BstV1I GCAGC 4 cut(s) 16, 355, 358, 387
Bsu15I ATCGAT 1 cut(s) 312
BsuI GTATCC 1 cut(s) 912
BsuRI GGCC 6 cut(s) 433, 686, 814, 832, 842, 897
BsuTUI ATCGAT 1 cut(s) 312
BtsCI GGATG 3 cut(s) 281, 604, 1021
BtsIMutI CAGTG 3 cut(s) 115, 265, 493
Cac8I GCNNGC 4 cut(s) 420, 770, 802, 885
CciI TCATGA 1 cut(s) 532
Cfr10I RCCGGY 2 cut(s) 205, 365
Cfr13I GGNCC 5 cut(s) 684, 812, 830, 841, 896
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 1 cut(s) 599
Csp6I GTAC 7 cut(s) 19, 678, 916, 934, 958, 969, 975
CviAII CATG 7 cut(s) 243, 515, 533, 838, 859, 884, 1000
CviQI GTAC 7 cut(s) 19, 678, 916, 934, 958, 969, 975
DdeI CTNAG 1 cut(s) 941
DpnI GATC 5 cut(s) 31, 507, 531, 537, 996
DpnII GATC 5 cut(s) 29, 505, 529, 535, 994
DraIII CACNNNGTG 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 431
EciI GGCGGA 3 cut(s) 395, 678, 780
Eco105I TACGTA 1 cut(s) 1014
Eco57I CTGAAG 3 cut(s) 231, 287, 413
EcoRII CCWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 1005
FaeI CATG 7 cut(s) 246, 518, 536, 841, 862, 887, 1003
FaqI GGGAC 1 cut(s) 737
FatI CATG 7 cut(s) 242, 514, 532, 837, 858, 883, 999
FauI CCCGC 1 cut(s) 793
FbaI TGATCA 2 cut(s) 529, 535
Fnu4HI GCNGC 7 cut(s) 5, 344, 347, 401, 658, 661, 745
FokI GGATG 3 cut(s) 288, 611, 1008
Fsp4HI GCNGC 7 cut(s) 5, 344, 347, 401, 658, 661, 745
FspBI CTAG 1 cut(s) 636
GluI GCNGC 7 cut(s) 5, 344, 347, 401, 658, 661, 745
HaeIII GGCC 6 cut(s) 433, 686, 814, 832, 842, 897
HapII CCGG 6 cut(s) 123, 206, 366, 459, 542, 765
HgaI GACGC 1 cut(s) 599
Hin1II CATG 7 cut(s) 246, 518, 536, 841, 862, 887, 1003
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HinfI GANTC 4 cut(s) 338, 385, 584, 862
HpaII CCGG 6 cut(s) 123, 206, 366, 459, 542, 765
HphI GGTGA 4 cut(s) 151, 539, 572, 593
Hpy166II GTNNAC 2 cut(s) 322, 1090
Hpy188I TCNGA 4 cut(s) 306, 589, 1026, 1054
Hpy188III TCNNGA 6 cut(s) 85, 250, 292, 533, 866, 992
Hpy8I GTNNAC 2 cut(s) 322, 1090
Hpy99I CGWCG 3 cut(s) 331, 578, 593
HpyAV CCTTC 3 cut(s) 266, 599, 608
HpyCH4III ACNGT 7 cut(s) 18, 92, 119, 269, 361, 488, 646
HpyCH4IV ACGT 4 cut(s) 372, 932, 1013, 1082
HpyCH4V TGCA 4 cut(s) 4, 279, 403, 1003
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 215, 397, 424
HpyF3I CTNAG 1 cut(s) 941
HpySE526I ACGT 4 cut(s) 372, 932, 1013, 1082
Hsp92II CATG 7 cut(s) 246, 518, 536, 841, 862, 887, 1003
Ksp22I TGATCA 2 cut(s) 529, 535
Kzo9I GATC 5 cut(s) 29, 505, 529, 535, 994
LmnI GCTCC 4 cut(s) 135, 223, 793, 994
Lsp1109I GCAGC 4 cut(s) 16, 355, 358, 387
LweI GCATC 3 cut(s) 266, 556, 775
MaeI CTAG 1 cut(s) 636
MaeII ACGT 4 cut(s) 372, 932, 1013, 1082
MaeIII GTNAC 7 cut(s) 12, 113, 157, 355, 361, 608, 944
MalI GATC 5 cut(s) 31, 507, 531, 537, 996
MboI GATC 5 cut(s) 29, 505, 529, 535, 994
MhlI GDGCHC 1 cut(s) 774
MluCI AATT 2 cut(s) 730, 775
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 2 cut(s) 612, 929
Mph1103I ATGCAT 1 cut(s) 1005
MseI TTAA 2 cut(s) 230, 441
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 6 cut(s) 123, 206, 366, 459, 542, 765
MspR9I CCNGG 3 cut(s) 222, 460, 765
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 4 cut(s) 206, 215, 397, 424
NciI CCSGG 2 cut(s) 460, 765
NdeII GATC 5 cut(s) 29, 505, 529, 535, 994
NlaIII CATG 7 cut(s) 246, 518, 536, 841, 862, 887, 1003
NlaIV GGNNCC 2 cut(s) 226, 685
NmeAIII GCCGAG 1 cut(s) 448
NmuCI GTSAC 4 cut(s) 12, 113, 157, 355
NsiI ATGCAT 1 cut(s) 1005
NspI RCATGY 1 cut(s) 887
PaeI GCATGC 1 cut(s) 887
PagI TCATGA 1 cut(s) 532
PfeI GAWTC 3 cut(s) 338, 584, 862
Pfl23II CGTACG 1 cut(s) 933
PfoI TCCNGGA 2 cut(s) 220, 458
PkrI GCNGC 7 cut(s) 6, 345, 348, 402, 659, 662, 746
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Ppu21I YACGTR 1 cut(s) 1014
PshAI GACNNNNGTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspLI CGTACG 1 cut(s) 933
PspN4I GGNNCC 2 cut(s) 226, 685
PspPI GGNCC 5 cut(s) 684, 812, 830, 841, 896
PstI CTGCAG 1 cut(s) 405
RsaI GTAC 7 cut(s) 20, 679, 917, 935, 959, 970, 976
RsaNI GTAC 7 cut(s) 19, 678, 916, 934, 958, 969, 975
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 230, 441
SatI GCNGC 7 cut(s) 5, 344, 347, 401, 658, 661, 745
Sau3AI GATC 5 cut(s) 29, 505, 529, 535, 994
Sau96I GGNCC 5 cut(s) 684, 812, 830, 841, 896
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 3 cut(s) 222, 460, 765
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 3 cut(s) 266, 556, 775
SfcI CTRYAG 1 cut(s) 401
SmiMI CAYNNNNRTG 1 cut(s) 513
SnaBI TACGTA 1 cut(s) 1014
SphI GCATGC 1 cut(s) 887
Sse9I AATT 2 cut(s) 730, 775
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 3 cut(s) 220, 458, 763
TaaI ACNGT 7 cut(s) 18, 92, 119, 269, 361, 488, 646
TaiI ACGT 4 cut(s) 375, 935, 1016, 1085
TaqI TCGA 3 cut(s) 28, 312, 449
TasI AATT 2 cut(s) 730, 775
TatI WGTACW 4 cut(s) 18, 677, 915, 968
TauI GCSGC 3 cut(s) 660, 663, 747
TfiI GAWTC 3 cut(s) 338, 584, 862
Tru1I TTAA 2 cut(s) 230, 441
Tru9I TTAA 2 cut(s) 230, 441
TscAI CASTG 3 cut(s) 122, 272, 493
TseFI GTSAC 4 cut(s) 12, 113, 157, 355
TseI GCWGC 4 cut(s) 4, 343, 346, 400
Tsp45I GTSAC 4 cut(s) 12, 113, 157, 355
TspDTI ATGAA 5 cut(s) 123, 531, 875, 1089, 1110
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 3 cut(s) 122, 272, 493
XceI RCATGY 1 cut(s) 887
XspI CTAG 1 cut(s) 636
ZrmI AGTACT 1 cut(s) 679
Zsp2I ATGCAT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.