pycom12401g00260

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0016965
Physical Location & Seq
Forward (+)
149805 .. 150014
210 bp
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UTR
Exon/CDS
Intron
pycom12401g00260.1

Sequence Viewer

Length: 210 bp
ATGGAAGCATCCATGAGTTGTGTCCTTGATCAGCGCGATGAAACGGGTAGGAAGGAAAGAGCCATATACTATCTCAGCAAGAAGTTCACTGATTATGAGACAAGGTATACGGTTCTAGAAAAGACCTGTTGTGCTCTTACACGGGCCTCGCAAGGCCTACGCCACTACATGTTGAACTATACGACCATGCTGCAAGGATGGGCCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-patch RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

70

Amino Acids

8.18

Weight (kDa)

8.88

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH PF17917 4 - 61 9.2e-13 RNase H-like domain found in reverse transcriptase
RT_RNaseH_2 PF17919 4 - 62 4e-11 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 107
AccII CGCG 1 cut(s) 36
AciI CCGC 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 1 cut(s) 175
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 92
AoxI GGCC 3 cut(s) 144, 154, 201
ApaI GGGCCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 190
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 3 cut(s) 144, 201, 202
BaeGI GKGCMC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 177
BccI CCATC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 116
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 144, 201, 202
BmiI GGNNCC 1 cut(s) 203
BmsI GCATC 1 cut(s) 17
BseGI GGATG 2 cut(s) 8, 203
BseMII CTCAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 36
BshFI GGCC 3 cut(s) 146, 156, 203
BsiHKAI GWGCWC 1 cut(s) 136
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 3 cut(s) 146, 156, 203
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 136, 205
Bsp143I GATC 1 cut(s) 28
BspACI CCGC 1 cut(s) 205
BspANI GGCC 3 cut(s) 146, 156, 203
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 1 cut(s) 36
BspLI GGNNCC 1 cut(s) 203
BssMI GATC 1 cut(s) 28
BssNAI GTATAC 1 cut(s) 108
Bst1107I GTATAC 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 74
BstF5I GGATG 2 cut(s) 8, 203
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 1 cut(s) 31
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 172
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 177
BstZ17I GTATAC 1 cut(s) 108
BsuRI GGCC 3 cut(s) 146, 156, 203
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 8, 203
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 205
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 3 cut(s) 144, 201, 202
CviAII CATG 3 cut(s) 13, 169, 187
CviJI RGCY 4 cut(s) 62, 146, 156, 203
CviKI_1 RGCY 4 cut(s) 62, 146, 156, 203
DdeI CTNAG 1 cut(s) 74
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 3 cut(s) 16, 172, 190
FaiI YATR 8 cut(s) 14, 65, 67, 96, 108, 170, 180, 188
FatI CATG 3 cut(s) 12, 168, 186
FbaI TGATCA 1 cut(s) 28
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 1 cut(s) 191
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 1 cut(s) 191
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 191
HaeIII GGCC 3 cut(s) 146, 156, 203
HhaI GCGC 1 cut(s) 36
Hin1II CATG 3 cut(s) 16, 172, 190
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 87, 108
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 2 cut(s) 87, 108
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 1 cut(s) 193
HpyF3I CTNAG 1 cut(s) 74
Hsp92II CATG 3 cut(s) 16, 172, 190
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 1 cut(s) 28
LpnPI CCDG 1 cut(s) 139
Lsp1109I GCAGC 1 cut(s) 177
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 116
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 136, 205
MnlI CCTC 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 207
MvnI CGCG 1 cut(s) 36
NdeII GATC 1 cut(s) 28
NlaIII CATG 3 cut(s) 16, 172, 190
NlaIV GGNNCC 1 cut(s) 203
NspI RCATGY 1 cut(s) 172
PceI AGGCCT 1 cut(s) 156
PciI ACATGT 1 cut(s) 168
PkrI GCNGC 1 cut(s) 192
PscI ACATGT 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 203
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 144, 201, 202
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 3 cut(s) 144, 201, 202
SduI GDGCHC 2 cut(s) 136, 205
SetI ASST 2 cut(s) 107, 128
SfaNI GCATC 1 cut(s) 17
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 1 cut(s) 205
SspMI CTAG 1 cut(s) 116
StuI AGGCCT 1 cut(s) 156
TaaI ACNGT 1 cut(s) 112
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 94
XbaI TCTAGA 1 cut(s) 115
XceI RCATGY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.