pycom12426g00460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012426
OG0015723
Physical Location & Seq
Forward (+)
327339 .. 328070
732 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12426g00460.1

Sequence Viewer

Length: 540 bp
ATGAAATCAATACCCCAAACATCAAAAATTTCCACAAATGAGAGAGATTGTTGCGGCATTTGCTCCTTAGGCCCTATGTTACCTGCCAATAAAATCCAGATGGTAACACCTTTAGGGCTGTCCTTTGAGTGCCAAAATGTCCCCCACATTCGGGCACACATCTACGCACAATCTGATCTGGGCAGAATTTCCATAAATATGGATCATCCCAAACATAAAAACGTGCATCATGAACAAGTTTATCACAAATTGACCAAATCGGCATACCAAGGGGTTCTTACCTCAATGGACAGAAGTTGCTCGTCCGAAAACGTTTCTAAAATGGGGGTGGACTCTTCCTCACGCACCATTCTACTTAGGTGGTCAGCCACCACTAGGAGCATCCAACGAATGAGTCTTGGCTTAGCCTCCTTTTTGGTGAGAAGATACTTCAAGGCTGCATGGTCAGTGAAAACAATTACTTTAGTACCAAGAAGATATGATCTAAACTTATCTAATGCAAATACAATAGCCAAGAGTTCTTTTTCGGTTGTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

20.06

Weight (kDa)

9.84

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 1 cut(s) 54
AclI AACGTT 1 cut(s) 312
AclWI GGATC 1 cut(s) 210
AcsI RAATTY 2 cut(s) 27, 186
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 3 cut(s) 150, 151, 375
AgsI TTSAA 1 cut(s) 433
AlwI GGATC 1 cut(s) 210
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 27, 186
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 450, 483
BbvI GCAGC 1 cut(s) 424
BccI CCATC 1 cut(s) 94
BfaI CTAG 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 91
BisI GCNGC 2 cut(s) 55, 438
BlpI GCTNAGC 1 cut(s) 403
BlsI GCNGC 2 cut(s) 56, 439
BmgT120I GGNCC 1 cut(s) 71
BmsI GCATC 2 cut(s) 235, 390
Bpu1102I GCTNAGC 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 268
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 151, 375
Bse21I CCTNAGG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 2 cut(s) 205, 381
BseLI CCNNNNNNNGG 3 cut(s) 150, 151, 375
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 1 cut(s) 72
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 3 cut(s) 150, 151, 375
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 3 cut(s) 175, 202, 481
Bsp1720I GCTNAGC 1 cut(s) 403
BspACI CCGC 1 cut(s) 54
BspANI GGCC 1 cut(s) 72
BspHI TCATGA 1 cut(s) 229
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 3 cut(s) 175, 202, 481
BssT1I CCWWGG 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 340
BstDEI CTNAG 3 cut(s) 67, 356, 403
BstF5I GGATG 2 cut(s) 205, 381
BstKTI GATC 3 cut(s) 178, 205, 484
BstMBI GATC 3 cut(s) 175, 202, 481
BstMWI GCNNNNNNNGC 2 cut(s) 60, 69
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 67
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 205, 381
BtsIMutI CAGTG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 91
CciI TCATGA 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 467
CviAII CATG 2 cut(s) 230, 441
CviJI RGCY 7 cut(s) 72, 118, 368, 402, 407, 437, 512
CviKI_1 RGCY 7 cut(s) 72, 118, 368, 402, 407, 437, 512
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 67, 356, 403
DpnI GATC 3 cut(s) 177, 204, 483
DpnII GATC 3 cut(s) 175, 202, 481
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 268
Eco81I CCTNAGG 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 2 cut(s) 233, 444
FaiI YATR 8 cut(s) 77, 194, 200, 216, 231, 265, 442, 480
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 1 cut(s) 125
FatI CATG 2 cut(s) 229, 440
Fnu4HI GCNGC 2 cut(s) 55, 438
FokI GGATG 2 cut(s) 192, 368
Fsp4HI GCNGC 2 cut(s) 55, 438
FspBI CTAG 1 cut(s) 375
GluI GCNGC 2 cut(s) 55, 438
HaeIII GGCC 1 cut(s) 72
Hin1II CATG 2 cut(s) 233, 444
HinfI GANTC 2 cut(s) 332, 394
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 2 cut(s) 175, 307
Hpy188III TCNNGA 2 cut(s) 97, 230
Hpy8I GTNNAC 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 222, 312
HpyCH4V TGCA 3 cut(s) 226, 440, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 69
HpyF3I CTNAG 3 cut(s) 67, 356, 403
HpySE526I ACGT 2 cut(s) 222, 312
Hsp92II CATG 2 cut(s) 233, 444
Kzo9I GATC 3 cut(s) 175, 202, 481
LmnI GCTCC 2 cut(s) 68, 378
LpnPI CCDG 3 cut(s) 96, 110, 164
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 2 cut(s) 235, 390
MaeI CTAG 1 cut(s) 375
MaeII ACGT 2 cut(s) 222, 312
MaeIII GTNAC 2 cut(s) 78, 103
MalI GATC 3 cut(s) 177, 204, 483
MboI GATC 3 cut(s) 175, 202, 481
MboII GAAGA 3 cut(s) 327, 435, 486
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 4 cut(s) 27, 186, 248, 456
MlyI GAGTC 2 cut(s) 326, 403
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 3 cut(s) 292, 349, 418
MslI CAYNNNNRTG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 60, 69
NdeII GATC 3 cut(s) 175, 202, 481
NlaIII CATG 2 cut(s) 233, 444
PagI TCATGA 1 cut(s) 229
PkrI GCNGC 2 cut(s) 56, 439
PleI GAGTC 2 cut(s) 326, 402
PpsI GAGTC 2 cut(s) 326, 402
Psp1406I AACGTT 1 cut(s) 312
PspPI GGNCC 1 cut(s) 71
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 197
SatI GCNGC 2 cut(s) 55, 438
Sau3AI GATC 3 cut(s) 175, 202, 481
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 2 cut(s) 326, 403
SduI GDGCHC 1 cut(s) 157
SetI ASST 6 cut(s) 85, 112, 225, 284, 315, 362
SfaNI GCATC 2 cut(s) 235, 390
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 4 cut(s) 27, 186, 248, 456
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 268
TaiI ACGT 2 cut(s) 225, 315
TasI AATT 4 cut(s) 27, 186, 248, 456
TauI GCSGC 1 cut(s) 57
TscAI CASTG 1 cut(s) 453
TseI GCWGC 1 cut(s) 437
TspDTI ATGAA 2 cut(s) 17, 246
TspRI CASTG 1 cut(s) 453
XapI RAATTY 2 cut(s) 27, 186
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.