pycom436g00460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0015723
Physical Location & Seq
Forward (+)
520710 .. 522825
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00460.7

Sequence Viewer

Length: 1446 bp
ATGTTTACAACATTCTATAATAGTACCCTAAGACACAAAAACAACTCTTTTTGGGTTTTTTTGAAACAATTTTTCAAATTTTTATGGGATTTTCGAATTTTTAAGTCAAAACACACTAAAACATTATGGTGGATAAAACCCTACGAATTTGCATCAAAACACTTTAGTTACCCCCCACACTTAAATCAAACATTGTCCTCAATGTTTCAACAACAAAGAGAAGAGTTTAGGAACGCAAATCTGGAATTGGAGTGCTTTCTAGGTCTTCCTTTGTTGAATGCATGGGTTGCCTCCCAAGTAGCGCTTGCTTTAACGTCGTACAGCCGGACGAAGAACCCAATCACTCCAGATTGGAGCCCACGGCACCCAAGGGAAGCTCTTCCACAACATGCTCCACAAAGTTCTCATAGTATGCTTTGGACTTGGACTAACGTAACTTACCAGGGAATAACCGAAGTCTTGAATCAAAAAGCAACACTTTCTGCCCCTTGGAGAACGTTTTGGTACAAAGCATCTTATCATGATAAGCCTTCGTCTTGTCCTTGTAGATGCGTGCATTCTCGTAAGCCTCCAGCGTCTATGTCCAAATTGAAGGTTTTCACAGCCCAATGTGCCTTGTCCGAAAAGGTGACATGCCAAGGGGAGTTTTGTAAGCCGTACGATAAGCCCACAATGCATCGTCCAAGCGCAAACTCCAATCTCTTCTTGAGGGTCCCACGTAGTTTGAGGGTGATAAGGTGTGGAAACCTTATGAGTAACGTTGTACTTCTTGATAAAACTCGTGGCATCCCAAACCTAGCAAAAATATTAGATTTCACAAAATCTGCAACAACCTTAGAATCGTTAGTACGGGTGCAAGATATAAGTGAAACCAAAAGATGGAGGGAAGGGGCCCATGAAATCAATACCCCAAACATCAAAAATTTCCACAAAGGAGAGAGATTGTTGCGGCATTTGGTCCTTAGGCCCTATGTTACCTGTTCTTTGACACTTATCGCCAATAAAATCCAGATTGTAACACCTTTAGGGCTGTCCTTTGGGTGCCAAAATGTCCCCCACATGCATAACTACGCACAATCTGATCTGGGCAGAATTTCCATAAATATGGATCATCCCAAACATAAAAACGTGCATCATGAACAAGTTTATCACAAATTGACCAAATCGGCATACCAAGGGGTTCTTACCTCAATGGACAGAAGTTGCTCATCCGGAAACGTTTCTAAAATGGGGGTGGACTCTTCCTCACGCACCATTCTACTTAGGTGGTCAGCCACCACTAGGAGCATCCAACGAATGAGTCTTGGCTTAGCCTCCTTTTTGGTGAGAAGATACTTCAAGGCTGCATGGTCAGTGAAAACAATTACTTTAGTACCAAGAAGATATGATCTAAACTTATCTAATGCAAATACAATAGCCAAGAGTTCTTTTTCGGTTGTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

482

Amino Acids

55.46

Weight (kDa)

10.07

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 363, 1041
AccB7I CCANNNNNTGG 1 cut(s) 879
AccIII TCCGGA 1 cut(s) 1211
AciI CCGC 1 cut(s) 949
AclI AACGTT 3 cut(s) 497, 759, 1218
AclWI GGATC 1 cut(s) 1116
AcsI RAATTY 5 cut(s) 77, 96, 146, 922, 1092
AfaI GTAC 7 cut(s) 25, 320, 506, 659, 765, 849, 1374
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 2 cut(s) 879, 1281
AgsI TTSAA 7 cut(s) 64, 76, 209, 277, 463, 592, 1339
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
AlwI GGATC 1 cut(s) 1116
Aor13HI TCCGGA 1 cut(s) 1211
Aor51HI AGCGCT 1 cut(s) 303
AoxI GGCC 2 cut(s) 891, 965
ApaI GGGCCC 1 cut(s) 895
ApeKI GCWGC 1 cut(s) 1343
ApoI RAATTY 5 cut(s) 77, 96, 146, 922, 1092
Asp700I GAANNNNTTC 3 cut(s) 378, 596, 1219
AspLEI GCGC 2 cut(s) 304, 689
AspS9I GGNCC 5 cut(s) 712, 891, 892, 958, 966
AsuHPI GGTGA 3 cut(s) 640, 742, 1336
AsuII TTCGAA 1 cut(s) 94
AvaII GGWCC 2 cut(s) 712, 958
AxyI CCTNAGG 1 cut(s) 962
BaeGI GKGCMC 1 cut(s) 895
BaeI ACNNNNGTAYC 2 cut(s) 1356, 1389
BanI GGYRCC 2 cut(s) 363, 1041
BanII GRGCYC 2 cut(s) 359, 895
BauI CACGAG 1 cut(s) 780
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 1 cut(s) 1330
BccI CCATC 1 cut(s) 873
BceAI ACGGC 2 cut(s) 377, 640
BciT130I CCWGG 1 cut(s) 443
BfaI CTAG 3 cut(s) 260, 797, 1281
BfoI RGCGCY 1 cut(s) 305
BisI GCNGC 2 cut(s) 950, 1344
BlpI GCTNAGC 1 cut(s) 1309
BlsI GCNGC 2 cut(s) 951, 1345
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 2 cut(s) 712, 958
BmgT120I GGNCC 5 cut(s) 712, 891, 892, 958, 966
BmiI GGNNCC 7 cut(s) 356, 365, 713, 714, 892, 893, 1043
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 7 cut(s) 161, 521, 539, 685, 795, 1141, 1296
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 330, 555
Bpu1102I GCTNAGC 1 cut(s) 1309
Bpu14I TTCGAA 1 cut(s) 94
BpuEI CTTGAG 1 cut(s) 727
BsaAI YACGTR 1 cut(s) 719
BsaJI CCNNGG 6 cut(s) 359, 368, 442, 488, 637, 1174
BsaWI WCCGGW 1 cut(s) 1211
Bsc4I CCNNNNNNNGG 2 cut(s) 879, 1281
Bse21I CCTNAGG 1 cut(s) 962
BseAI TCCGGA 1 cut(s) 1211
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 6 cut(s) 359, 368, 442, 488, 637, 1174
BseGI GGATG 4 cut(s) 786, 1111, 1208, 1287
BseLI CCNNNNNNNGG 2 cut(s) 879, 1281
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 1 cut(s) 1330
BshFI GGCC 2 cut(s) 893, 967
BshNI GGYRCC 2 cut(s) 363, 1041
BsiSI CCGG 2 cut(s) 325, 1212
BsiWI CGTACG 1 cut(s) 657
BslFI GGGAC 2 cut(s) 698, 1037
BslI CCNNNNNNNGG 2 cut(s) 879, 1281
BsmFI GGGAC 2 cut(s) 698, 1037
BsmI GAATGC 2 cut(s) 283, 556
BsnI GGCC 2 cut(s) 893, 967
Bsp119I TTCGAA 1 cut(s) 94
Bsp120I GGGCCC 1 cut(s) 891
Bsp1286I GDGCHC 2 cut(s) 359, 895
Bsp13I TCCGGA 1 cut(s) 1211
Bsp143I GATC 3 cut(s) 1081, 1108, 1387
Bsp1720I GCTNAGC 1 cut(s) 1309
BspACI CCGC 1 cut(s) 949
BspANI GGCC 2 cut(s) 893, 967
BspEI TCCGGA 1 cut(s) 1211
BspHI TCATGA 2 cut(s) 520, 1135
BspLI GGNNCC 7 cut(s) 356, 365, 713, 714, 892, 893, 1043
BspPI GGATC 1 cut(s) 1116
BspQI GCTCTTC 1 cut(s) 384
BspT104I TTCGAA 1 cut(s) 94
BspT107I GGYRCC 2 cut(s) 363, 1041
BssECI CCNNGG 6 cut(s) 359, 368, 442, 488, 637, 1174
BssMI GATC 3 cut(s) 1081, 1108, 1387
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 4 cut(s) 368, 488, 637, 1174
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 1 cut(s) 443
Bst6I CTCTTC 4 cut(s) 216, 384, 707, 1246
BstAPI GCANNNNNTGC 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 719
BstBI TTCGAA 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 306, 554
BstDEI CTNAG 5 cut(s) 29, 835, 962, 1262, 1309
BstDSI CCRYGG 1 cut(s) 359
BstF5I GGATG 4 cut(s) 786, 1111, 1208, 1287
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 304, 689
BstKTI GATC 3 cut(s) 1084, 1111, 1390
BstMBI GATC 3 cut(s) 1081, 1108, 1387
BstMWI GCNNNNNNNGC 3 cut(s) 287, 611, 673
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 3 cut(s) 392, 636, 1063
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 895
BstV1I GCAGC 1 cut(s) 1330
BstV2I GAAGAC 1 cut(s) 257
BstXI CCANNNNNNTGG 1 cut(s) 1105
Bsu36I CCTNAGG 1 cut(s) 962
BsuRI GGCC 2 cut(s) 893, 967
BtgI CCRYGG 1 cut(s) 359
BtsCI GGATG 4 cut(s) 786, 1111, 1208, 1287
BtsIMutI CAGTG 1 cut(s) 1359
Cac8I GCNNGC 2 cut(s) 306, 554
CciI TCATGA 2 cut(s) 520, 1135
CfoI GCGC 2 cut(s) 304, 689
Cfr13I GGNCC 5 cut(s) 712, 891, 892, 958, 966
CseI GACGC 1 cut(s) 564
Csp6I GTAC 7 cut(s) 24, 319, 505, 658, 764, 848, 1373
CspCI CAANNNNNGTGG 2 cut(s) 705, 740
CviAII CATG 8 cut(s) 282, 389, 521, 633, 896, 1060, 1136, 1347
CviQI GTAC 7 cut(s) 24, 319, 505, 658, 764, 848, 1373
DdeI CTNAG 5 cut(s) 29, 835, 962, 1262, 1309
DpnI GATC 3 cut(s) 1083, 1110, 1389
DpnII GATC 3 cut(s) 1081, 1108, 1387
Eam1104I CTCTTC 4 cut(s) 216, 384, 707, 1246
EarI CTCTTC 4 cut(s) 216, 384, 707, 1246
Eco130I CCWWGG 4 cut(s) 368, 488, 637, 1174
Eco24I GRGCYC 2 cut(s) 359, 895
Eco47I GGWCC 2 cut(s) 712, 958
Eco47III AGCGCT 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 962
EcoO109I RGGNCCY 3 cut(s) 712, 891, 966
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 4 cut(s) 368, 488, 637, 1174
EcoT22I ATGCAT 3 cut(s) 283, 678, 1065
EcoT38I GRGCYC 2 cut(s) 359, 895
ErhI CCWWGG 4 cut(s) 368, 488, 637, 1174
FaeI CATG 8 cut(s) 285, 392, 524, 636, 899, 1063, 1139, 1350
FalI AAGNNNNNCTT 6 cut(s) 288, 320, 462, 494, 1167, 1199
FaqI GGGAC 2 cut(s) 698, 1037
FatI CATG 8 cut(s) 281, 388, 520, 632, 895, 1059, 1135, 1346
Fnu4HI GCNGC 2 cut(s) 950, 1344
FokI GGATG 4 cut(s) 773, 1098, 1195, 1274
FriOI GRGCYC 2 cut(s) 359, 895
Fsp4HI GCNGC 2 cut(s) 950, 1344
FspBI CTAG 3 cut(s) 260, 797, 1281
GlaI GCGC 2 cut(s) 303, 688
GluI GCNGC 2 cut(s) 950, 1344
GsuI CTGGAG 2 cut(s) 330, 555
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 2 cut(s) 893, 967
HapII CCGG 2 cut(s) 325, 1212
HgaI GACGC 1 cut(s) 564
HhaI GCGC 2 cut(s) 304, 689
Hin1II CATG 8 cut(s) 285, 392, 524, 636, 899, 1063, 1139, 1350
Hin6I GCGC 2 cut(s) 302, 687
HinP1I GCGC 2 cut(s) 302, 687
HinfI GANTC 4 cut(s) 463, 839, 1238, 1300
HpaII CCGG 2 cut(s) 325, 1212
HphI GGTGA 3 cut(s) 640, 742, 1336
Hpy166II GTNNAC 2 cut(s) 6, 1237
Hpy188I TCNGA 2 cut(s) 622, 1081
Hpy188III TCNNGA 9 cut(s) 242, 347, 460, 521, 706, 770, 1009, 1136, 1212
Hpy8I GTNNAC 2 cut(s) 6, 1237
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 3 cut(s) 540, 586, 881
HpyCH4IV ACGT 7 cut(s) 314, 432, 497, 718, 759, 1128, 1218
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 611, 673
HpyF3I CTNAG 5 cut(s) 29, 835, 962, 1262, 1309
HpySE526I ACGT 7 cut(s) 314, 432, 497, 718, 759, 1128, 1218
Hsp92II CATG 8 cut(s) 285, 392, 524, 636, 899, 1063, 1139, 1350
HspAI GCGC 2 cut(s) 302, 687
KflI GGGWCCC 1 cut(s) 712
Kpn2I TCCGGA 1 cut(s) 1211
Kzo9I GATC 3 cut(s) 1081, 1108, 1387
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 3 cut(s) 354, 397, 1284
Lsp1109I GCAGC 1 cut(s) 1330
LweI GCATC 7 cut(s) 161, 521, 539, 685, 795, 1141, 1296
MaeI CTAG 3 cut(s) 260, 797, 1281
MaeII ACGT 7 cut(s) 314, 432, 497, 718, 759, 1128, 1218
MaeIII GTNAC 6 cut(s) 167, 433, 628, 755, 973, 1015
MalI GATC 3 cut(s) 1083, 1110, 1389
MboI GATC 3 cut(s) 1081, 1108, 1387
MboII GAAGA 8 cut(s) 233, 257, 343, 371, 694, 1233, 1341, 1392
MhlI GDGCHC 2 cut(s) 359, 895
MlyI GAGTC 2 cut(s) 1232, 1309
MmeI TCCRAC 1 cut(s) 1315
MnlI CCTC 9 cut(s) 208, 301, 579, 702, 720, 876, 1198, 1255, 1324
Mph1103I ATGCAT 3 cut(s) 283, 678, 1065
MroI TCCGGA 1 cut(s) 1211
MroXI GAANNNNTTC 3 cut(s) 378, 596, 1219
MseI TTAA 3 cut(s) 102, 182, 311
MslI CAYNNNNRTG 2 cut(s) 127, 1103
MspI CCGG 2 cut(s) 325, 1212
MspR9I CCNGG 1 cut(s) 443
Mva1269I GAATGC 2 cut(s) 283, 556
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 3 cut(s) 287, 611, 673
NdeII GATC 3 cut(s) 1081, 1108, 1387
NlaIII CATG 8 cut(s) 285, 392, 524, 636, 899, 1063, 1139, 1350
NlaIV GGNNCC 7 cut(s) 356, 365, 713, 714, 892, 893, 1043
NmuCI GTSAC 1 cut(s) 628
NsiI ATGCAT 3 cut(s) 283, 678, 1065
NspI RCATGY 3 cut(s) 392, 636, 1063
NspV TTCGAA 1 cut(s) 94
PagI TCATGA 2 cut(s) 520, 1135
PciSI GCTCTTC 1 cut(s) 384
PctI GAATGC 2 cut(s) 283, 556
PdmI GAANNNNTTC 3 cut(s) 378, 596, 1219
PfeI GAWTC 2 cut(s) 463, 839
Pfl23II CGTACG 1 cut(s) 657
PflMI CCANNNNNTGG 1 cut(s) 879
PkrI GCNGC 2 cut(s) 951, 1345
PleI GAGTC 2 cut(s) 1232, 1308
PpsI GAGTC 2 cut(s) 1232, 1308
Ppu21I YACGTR 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 712
Psp1406I AACGTT 3 cut(s) 497, 759, 1218
Psp5II RGGWCCY 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspLI CGTACG 1 cut(s) 657
PspN4I GGNNCC 7 cut(s) 356, 365, 713, 714, 892, 893, 1043
PspOMI GGGCCC 1 cut(s) 891
PspPI GGNCC 5 cut(s) 712, 891, 892, 958, 966
PspPPI RGGWCCY 1 cut(s) 712
RsaI GTAC 7 cut(s) 25, 320, 506, 659, 765, 849, 1374
RsaNI GTAC 7 cut(s) 24, 319, 505, 658, 764, 848, 1373
RseI CAYNNNNRTG 2 cut(s) 127, 1103
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 3 cut(s) 102, 182, 311
SatI GCNGC 2 cut(s) 950, 1344
Sau3AI GATC 3 cut(s) 1081, 1108, 1387
Sau96I GGNCC 5 cut(s) 712, 891, 892, 958, 966
SchI GAGTC 2 cut(s) 1232, 1309
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 2 cut(s) 359, 895
SfaNI GCATC 7 cut(s) 161, 521, 539, 685, 795, 1141, 1296
SfuI TTCGAA 1 cut(s) 94
SinI GGWCC 2 cut(s) 712, 958
SmiMI CAYNNNNRTG 2 cut(s) 127, 1103
SmlI CTYRAG 1 cut(s) 706
SmoI CTYRAG 1 cut(s) 706
SsiI CCGC 1 cut(s) 949
SspI AATATT 1 cut(s) 807
SspMI CTAG 3 cut(s) 260, 797, 1281
StyD4I CCNGG 1 cut(s) 441
StyI CCWWGG 4 cut(s) 368, 488, 637, 1174
TaiI ACGT 7 cut(s) 317, 435, 500, 721, 762, 1131, 1221
TaqI TCGA 1 cut(s) 94
TatI WGTACW 1 cut(s) 763
TauI GCSGC 1 cut(s) 952
TfiI GAWTC 2 cut(s) 463, 839
Tru1I TTAA 3 cut(s) 102, 182, 311
Tru9I TTAA 3 cut(s) 102, 182, 311
TscAI CASTG 1 cut(s) 1359
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 1 cut(s) 1343
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 2 cut(s) 912, 1152
TspRI CASTG 1 cut(s) 1359
Van91I CCANNNNNTGG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 712, 958
XapI RAATTY 5 cut(s) 77, 96, 146, 922, 1092
XceI RCATGY 3 cut(s) 392, 636, 1063
XmnI GAANNNNTTC 3 cut(s) 378, 596, 1219
XspI CTAG 3 cut(s) 260, 797, 1281
Zsp2I ATGCAT 3 cut(s) 283, 678, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.