pycom13g25400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015723
Physical Location & Seq
Forward (+)
22224006 .. 22225209
1204 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25400.8

Sequence Viewer

Length: 864 bp
ATGTACGAGAACAAGCCAAATGCATCTTTTTGCTCCTTTAGCCCTTTGGACCTACAAACACACGAAAATAGCTTAAAAACAAGCCATTTAAGTCGCATAAATATGCTCCTATCAAATTGTTCTCGTACATTTACGTTATGTTTCTTTAGTTATTTTAGTACTTTAAGCTATTTTCGCATTTTGGAGACTTTTGGAACCGTTGGACCAACAAGGAGGGATTGGGTAACTAAGTATGTTGATGCAAATTCGTTGAATTTTATCACCCATAACTTCAAGTCACCCATAACTTCAAGTGTTGTTCCTTGCTGTTTTAAAAATAACGGGCCAATCCATTTAGAACGCAACTTACCAGGAAACAAACGTAGGCGAGAGTTGAAAAGTAACACTTTCTGCCCAACTGTGAAGGTCTTGGTTCGAATCATTTTATCATGGAATGCTTTGGTCTTGCTCCTCAAGTTCATTCAATTGGAGCTTCCTATAAACTCCCGCTGCATCTATGTCCATGTTGAAAGTCTTCACGGCCCAATGTGCCTTACGAGCCGAAAAGGTGACATCCCAATGGGTGTTTTGTACGCCGTACGATATGCCCACAGTGCATCATCTAAACGTAAGCTCCAATCCCTCCTTGTTGGTCCAACGGTCTTCTCCAAAATTTGTTTGATTTCTCGGTTAGACACCTCGGCTTGGCCATTTCAACGCCTCAATGGTCCGATTGCAAAAGTGAGACCCTCCATCACTTATGATTACCCTCGGCATTCCAAACCTAGAAAAAATGTTAGTTTTCACGAAATCTGCAACCACCTTAGAATCGTTAGTACGGGTGGCTTTTGCTTCCACCCATTTAGAAACATAATCAACGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

32.82

Weight (kDa)

9.96

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 487
AcoI YGGCCR 1 cut(s) 686
AcsI RAATTY 3 cut(s) 244, 253, 651
AfaI GTAC 6 cut(s) 5, 127, 160, 572, 579, 817
AfiI CCNNNNNNNGG 1 cut(s) 684
AgsI TTSAA 7 cut(s) 253, 274, 291, 376, 464, 509, 695
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 4 cut(s) 72, 168, 472, 613
AluI AGCT 4 cut(s) 72, 168, 472, 613
Alw26I GTCTC 2 cut(s) 179, 718
AoxI GGCC 3 cut(s) 323, 520, 686
ApeKI GCWGC 1 cut(s) 489
ApoI RAATTY 3 cut(s) 244, 253, 651
Asp700I GAANNNNTTC 1 cut(s) 513
AspS9I GGNCC 6 cut(s) 49, 203, 323, 521, 632, 707
AsuHPI GGTGA 3 cut(s) 253, 270, 560
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 4 cut(s) 49, 203, 632, 707
BalI TGGCCA 1 cut(s) 688
BbsI GAAGAC 2 cut(s) 506, 634
BbvI GCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 740
BceAI ACGGC 2 cut(s) 535, 560
BciT130I CCWGG 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 179, 718
BfaI CTAG 2 cut(s) 765, 862
BisI GCNGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 491
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 4 cut(s) 49, 203, 632, 707
BmgT120I GGNCC 6 cut(s) 49, 203, 323, 521, 632, 707
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 4 cut(s) 32, 229, 501, 605
BpiI GAAGAC 2 cut(s) 506, 634
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 718
BsaJI CCNNGG 2 cut(s) 678, 749
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 678, 749
BseGI GGATG 1 cut(s) 552
BseLI CCNNNNNNNGG 1 cut(s) 684
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 1 cut(s) 476
BshFI GGCC 3 cut(s) 325, 522, 688
BsiWI CGTACG 1 cut(s) 577
BslI CCNNNNNNNGG 1 cut(s) 684
BsmAI GTCTC 2 cut(s) 179, 718
BsmI GAATGC 2 cut(s) 439, 754
BsnI GGCC 3 cut(s) 325, 522, 688
Bso31I GGTCTC 1 cut(s) 718
Bsp119I TTCGAA 1 cut(s) 415
BspACI CCGC 1 cut(s) 487
BspANI GGCC 3 cut(s) 325, 522, 688
BspLI GGNNCC 1 cut(s) 196
BspT104I TTCGAA 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 718
BssECI CCNNGG 2 cut(s) 678, 749
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 199, 400, 593, 640
BstBI TTCGAA 1 cut(s) 415
BstDEI CTNAG 2 cut(s) 228, 803
BstF5I GGATG 1 cut(s) 552
BstMAI GTCTC 2 cut(s) 179, 718
BstMWI GCNNNNNNNGC 5 cut(s) 39, 174, 528, 537, 593
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 476
BstV2I GAAGAC 2 cut(s) 506, 634
BsuRI GGCC 3 cut(s) 325, 522, 688
BtsCI GGATG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 598
Cfr13I GGNCC 6 cut(s) 49, 203, 323, 521, 632, 707
Csp6I GTAC 6 cut(s) 4, 126, 159, 571, 578, 816
CviAII CATG 2 cut(s) 429, 503
CviQI GTAC 6 cut(s) 4, 126, 159, 571, 578, 816
DdeI CTNAG 2 cut(s) 228, 803
DraI TTTAAA 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 718
Eco47I GGWCC 4 cut(s) 49, 203, 632, 707
EcoRII CCWGG 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 25
FaeI CATG 2 cut(s) 432, 506
FalI AAGNNNNNCTT 2 cut(s) 370, 402
FatI CATG 2 cut(s) 428, 502
FauI CCCGC 1 cut(s) 494
Fnu4HI GCNGC 1 cut(s) 490
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 1 cut(s) 490
FspBI CTAG 2 cut(s) 765, 862
GluI GCNGC 1 cut(s) 490
HaeIII GGCC 3 cut(s) 325, 522, 688
Hin1II CATG 2 cut(s) 432, 506
HinfI GANTC 2 cut(s) 417, 807
HphI GGTGA 3 cut(s) 253, 270, 560
Hpy188I TCNGA 1 cut(s) 711
Hpy188III TCNNGA 1 cut(s) 785
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 4 cut(s) 199, 400, 593, 640
HpyCH4IV ACGT 3 cut(s) 134, 361, 607
HpyCH4V TGCA 6 cut(s) 23, 242, 492, 596, 716, 795
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 174, 528, 537, 593
HpyF3I CTNAG 2 cut(s) 228, 803
HpySE526I ACGT 3 cut(s) 134, 361, 607
Hsp92II CATG 2 cut(s) 432, 506
LmnI GCTCC 5 cut(s) 38, 111, 453, 469, 618
LpnPI CCDG 2 cut(s) 336, 363
Lsp1109I GCAGC 1 cut(s) 476
LweI GCATC 4 cut(s) 32, 229, 501, 605
MaeI CTAG 2 cut(s) 765, 862
MaeII ACGT 3 cut(s) 134, 361, 607
MaeIII GTNAC 4 cut(s) 223, 276, 380, 548
MboII GAAGA 2 cut(s) 506, 634
MfeI CAATTG 1 cut(s) 464
MlsI TGGCCA 1 cut(s) 688
MluCI AATT 5 cut(s) 115, 244, 253, 464, 651
MluNI TGGCCA 1 cut(s) 688
MmeI TCCRAC 2 cut(s) 181, 659
MnlI CCTC 7 cut(s) 207, 461, 632, 688, 710, 739, 759
Mox20I TGGCCA 1 cut(s) 688
Mph1103I ATGCAT 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 513
MscI TGGCCA 1 cut(s) 688
MseI TTAA 4 cut(s) 74, 89, 164, 312
MslI CAYNNNNRTG 2 cut(s) 101, 557
Msp20I TGGCCA 1 cut(s) 688
MspA1I CMGCKG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 464
Mva1269I GAATGC 2 cut(s) 439, 754
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 5 cut(s) 39, 174, 528, 537, 593
NlaIII CATG 2 cut(s) 432, 506
NlaIV GGNNCC 1 cut(s) 196
NmeAIII GCCGAG 2 cut(s) 659, 730
NmuCI GTSAC 2 cut(s) 276, 548
NsiI ATGCAT 1 cut(s) 25
NspV TTCGAA 1 cut(s) 415
PctI GAATGC 2 cut(s) 439, 754
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 2 cut(s) 417, 807
Pfl23II CGTACG 1 cut(s) 577
PkrI GCNGC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspLI CGTACG 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 6 cut(s) 49, 203, 323, 521, 632, 707
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
RsaI GTAC 6 cut(s) 5, 127, 160, 572, 579, 817
RsaNI GTAC 6 cut(s) 4, 126, 159, 571, 578, 816
RseI CAYNNNNRTG 2 cut(s) 101, 557
SaqAI TTAA 4 cut(s) 74, 89, 164, 312
SatI GCNGC 1 cut(s) 490
Sau96I GGNCC 6 cut(s) 49, 203, 323, 521, 632, 707
ScaI AGTACT 1 cut(s) 160
ScrFI CCNGG 1 cut(s) 351
SfaNI GCATC 4 cut(s) 32, 229, 501, 605
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 4 cut(s) 49, 203, 632, 707
SmiMI CAYNNNNRTG 2 cut(s) 101, 557
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 5 cut(s) 115, 244, 253, 464, 651
SsiI CCGC 1 cut(s) 487
SspMI CTAG 2 cut(s) 765, 862
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 4 cut(s) 199, 400, 593, 640
TaiI ACGT 3 cut(s) 137, 364, 610
TaqI TCGA 1 cut(s) 415
TasI AATT 5 cut(s) 115, 244, 253, 464, 651
TatI WGTACW 1 cut(s) 158
TfiI GAWTC 2 cut(s) 417, 807
Tru1I TTAA 4 cut(s) 74, 89, 164, 312
Tru9I TTAA 4 cut(s) 74, 89, 164, 312
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 276, 548
TseI GCWGC 1 cut(s) 489
Tsp45I GTSAC 2 cut(s) 276, 548
TspDTI ATGAA 1 cut(s) 448
TspRI CASTG 1 cut(s) 598
VpaK11BI GGWCC 4 cut(s) 49, 203, 632, 707
XapI RAATTY 3 cut(s) 244, 253, 651
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 765, 862
ZrmI AGTACT 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.