pycom436g00280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0015723
Physical Location & Seq
Forward (+)
335625 .. 337849
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00280.6

Sequence Viewer

Length: 1551 bp
ATGATTGCATGTGAAGTCATAAGTTATAACCACAACCAAGCATACACAAAGAGTAAATCAACTTTCATCTATCCTCATCATTGTGTTGGAAGGTACCCTAAAACAAAAACAAACATACAAAAACACTTTGAAAACAACTCTTTTTGGGTTTTTAAAACAAATTTTTCAAATTTTTATGTGATTTTCGGATTTTTACTTCAAAACACACTAAAACACACCAAAACTGAATTTGCATCAAAAACACTTAGTTACCCCCCCCCCCCCACACTTAAATCAAACATTGTCCTCAATGTTTCAAGCATAAAATCACACAAAACACAAAGAAACACACAAACAGCAATGAAGGGTTTAGGAACTGCATGGGTTGCCTCCCAAGTAGTGCTTTCTTTAACGTCTTGCAGCCGGACGAAGACACGTTCATGCTCCATTGGAGCCCACGGCATGGAGTGGGATCTCCTCCACGACATGCTCCACAAAGTTCTCATAGTATGGCTTCAATCTATGTCCGTTCACCTTGAACTCGTGAACGTAACTTACCAGGGAATAACCGAAGTCTTGAATCAAAAAGCAACACTTTCTGCCCCTTGGAGAACGTTTTGGTACGAAGCATCTTATCATGATAAGCCTTCGTCTTGTCCTTGTAGATGCGAGCATTCTCGTAAGCCTCCAGCGTCTATGTCCAAATTGAAGGTTTTCACAGCCCAATGTGCCTTGTCCGAAAAGGTGACATGCCAAGGGGAGTTTTGTAAGCCGTACGATAGGCCCACAATGCATCGTCCAAGCGCAAACTCCAATCTCTTCTTGAGGGTCCCACGTGGTTTGAGGGTGATAAGGTGTGGAAACCTTATGAGTAACGTTGTACTTCTTGATAAAACTCGTGGCATCCCAAACCTAGCAAAAATATTAGATTTCACAAAATCTGCAACAACCTTAGAATCGTTAGTACGGGTGCAAGATATAAGTGAAACCAAAAGATGGAGGGAAGGGGCCCATGAAATCAATACCCCAAACATCAAAAATTTCCACAAAAGAGAGAGATTGTTGCGGCATTTGGTCCTTAGGCCCTATGTTACCTGTTCTTTGACACTTATCGCATGTTACACAAAACATTTTAGCATCTTTAAAGAGATTAGGCCAATAAAATCCAGATTAAGTATGGCAGAATGTAAGAATGGAATTAAATTCAGATTCGGGCACACATCTACTTTATCACGTTGGAACTTGTTTAGGGTACTAGGAACTTGCTTAGACACCAAGAAATTGACCAAATCGGCATACCAAGGGGTTCTTACCTCAATGGACAGAAGTTGCTCATCCGGAAATGTTTCTAAAATGGGGGTGGACTCTTCCTCACGCACCATTCTACTTAGGTGGTCAGCCACCACTAGGAGCATCCAACGAATGAGTCTTGGCTTAGCCTCCTTTTTGGTGAGAAGATATTTCAAGGCTGCATGGTCAGTGAAAACAATTACTTTAGTACCAAGAAGATATGATCTAAACTTATCTAAAGCAAATACAACCGCCAAAAGTTCTTTTTCGGTTGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

517

Amino Acids

58.82

Weight (kDa)

10.09

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 93
AccB7I CCANNNNNTGG 2 cut(s) 442, 975
AccIII TCCGGA 1 cut(s) 1316
AciI CCGC 2 cut(s) 1045, 1521
AclI AACGTT 2 cut(s) 593, 855
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 5 cut(s) 160, 169, 227, 1018, 1181
AcvI CACGTG 1 cut(s) 815
AfaI GTAC 7 cut(s) 95, 602, 755, 861, 945, 1233, 1479
AfiI CCNNNNNNNGG 3 cut(s) 442, 975, 1386
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 9 cut(s) 131, 168, 200, 297, 497, 518, 559, 688, 1444
AjnI CCWGG 1 cut(s) 537
AlwI GGATC 1 cut(s) 459
Aor13HI TCCGGA 1 cut(s) 1316
AoxI GGCC 4 cut(s) 761, 987, 1061, 1133
ApaI GGGCCC 1 cut(s) 991
ApeKI GCWGC 2 cut(s) 399, 1448
ApoI RAATTY 5 cut(s) 160, 169, 227, 1018, 1181
Asp700I GAANNNNTTC 2 cut(s) 692, 1324
Asp718I GGTACC 1 cut(s) 93
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 6 cut(s) 762, 808, 987, 988, 1054, 1062
AsuHPI GGTGA 4 cut(s) 503, 736, 838, 1441
AvaII GGWCC 2 cut(s) 808, 1054
AxyI CCTNAGG 1 cut(s) 1058
BaeGI GKGCMC 2 cut(s) 991, 1197
BaeI ACNNNNGTAYC 2 cut(s) 1461, 1494
BanI GGYRCC 1 cut(s) 93
BanII GRGCYC 2 cut(s) 436, 991
BauI CACGAG 2 cut(s) 521, 876
BbrPI CACGTG 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 2 cut(s) 411, 1435
BccI CCATC 1 cut(s) 969
BceAI ACGGC 2 cut(s) 454, 736
BciT130I CCWGG 1 cut(s) 539
BfaI CTAG 3 cut(s) 893, 1235, 1386
BisI GCNGC 3 cut(s) 400, 1046, 1449
BlpI GCTNAGC 1 cut(s) 1414
BlsI GCNGC 3 cut(s) 401, 1047, 1450
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 2 cut(s) 808, 1054
BmgT120I GGNCC 6 cut(s) 762, 808, 987, 988, 1054, 1062
BmiI GGNNCC 6 cut(s) 95, 433, 809, 810, 988, 989
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 7 cut(s) 242, 617, 635, 781, 891, 1125, 1401
BpiI GAAGAC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 651
Bpu1102I GCTNAGC 1 cut(s) 1414
BpuEI CTTGAG 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 815
BsaJI CCNNGG 5 cut(s) 436, 538, 584, 733, 1279
BsaWI WCCGGW 1 cut(s) 1316
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 975, 1386
Bse21I CCTNAGG 1 cut(s) 1058
Bse3DI GCAATG 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 1316
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 5 cut(s) 436, 538, 584, 733, 1279
BseGI GGATG 3 cut(s) 882, 1313, 1392
BseLI CCNNNNNNNGG 3 cut(s) 442, 975, 1386
BseMI GCAATG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 446
BseSI GKGCMC 2 cut(s) 991, 1197
BseXI GCAGC 2 cut(s) 411, 1435
BshFI GGCC 4 cut(s) 763, 989, 1063, 1135
BshNI GGYRCC 1 cut(s) 93
BsiSI CCGG 2 cut(s) 403, 1317
BsiWI CGTACG 1 cut(s) 753
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 3 cut(s) 442, 975, 1386
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 4 cut(s) 763, 989, 1063, 1135
Bsp120I GGGCCC 1 cut(s) 987
Bsp1286I GDGCHC 3 cut(s) 436, 991, 1197
Bsp13I TCCGGA 1 cut(s) 1316
Bsp143I GATC 2 cut(s) 451, 1492
Bsp1720I GCTNAGC 1 cut(s) 1414
BspACI CCGC 2 cut(s) 1045, 1521
BspANI GGCC 4 cut(s) 763, 989, 1063, 1135
BspEI TCCGGA 1 cut(s) 1316
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 6 cut(s) 95, 433, 809, 810, 988, 989
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 345
BssECI CCNNGG 5 cut(s) 436, 538, 584, 733, 1279
BssMI GATC 2 cut(s) 451, 1492
BssSI CACGAG 2 cut(s) 521, 876
BssT1I CCWWGG 3 cut(s) 584, 733, 1279
Bst2BI CACGAG 2 cut(s) 521, 876
Bst2UI CCWGG 1 cut(s) 539
Bst6I CTCTTC 2 cut(s) 803, 1351
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 1 cut(s) 650
BstDEI CTNAG 6 cut(s) 245, 931, 1058, 1246, 1367, 1414
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 882, 1313, 1392
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 2 cut(s) 454, 1495
BstMBI GATC 2 cut(s) 451, 1492
BstMWI GCNNNNNNNGC 3 cut(s) 365, 707, 769
BstNI CCWGG 1 cut(s) 539
BstNSI RCATGY 4 cut(s) 12, 469, 732, 1098
BstSCI CCNGG 1 cut(s) 537
BstSLI GKGCMC 2 cut(s) 991, 1197
BstV1I GCAGC 2 cut(s) 411, 1435
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
Bsu36I CCTNAGG 1 cut(s) 1058
BsuRI GGCC 4 cut(s) 763, 989, 1063, 1135
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 3 cut(s) 882, 1313, 1392
BtsIMutI CAGTG 1 cut(s) 1464
Cac8I GCNNGC 1 cut(s) 650
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 785
Cfr13I GGNCC 6 cut(s) 762, 808, 987, 988, 1054, 1062
CseI GACGC 1 cut(s) 660
Csp6I GTAC 7 cut(s) 94, 601, 754, 860, 944, 1232, 1478
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviQI GTAC 7 cut(s) 94, 601, 754, 860, 944, 1232, 1478
DdeI CTNAG 6 cut(s) 245, 931, 1058, 1246, 1367, 1414
DpnI GATC 2 cut(s) 453, 1494
DpnII GATC 2 cut(s) 451, 1492
DraI TTTAAA 2 cut(s) 154, 1123
Eam1104I CTCTTC 2 cut(s) 803, 1351
EarI CTCTTC 2 cut(s) 803, 1351
Eco130I CCWWGG 3 cut(s) 584, 733, 1279
Eco24I GRGCYC 2 cut(s) 436, 991
Eco47I GGWCC 2 cut(s) 808, 1054
Eco72I CACGTG 1 cut(s) 815
Eco81I CCTNAGG 1 cut(s) 1058
EcoO109I RGGNCCY 3 cut(s) 808, 987, 1062
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 584, 733, 1279
EcoT22I ATGCAT 1 cut(s) 774
EcoT38I GRGCYC 2 cut(s) 436, 991
ErhI CCWWGG 3 cut(s) 584, 733, 1279
FalI AAGNNNNNCTT 6 cut(s) 366, 398, 558, 590, 1272, 1304
FaqI GGGAC 1 cut(s) 794
Fnu4HI GCNGC 3 cut(s) 400, 1046, 1449
FokI GGATG 3 cut(s) 869, 1300, 1379
FriOI GRGCYC 2 cut(s) 436, 991
Fsp4HI GCNGC 3 cut(s) 400, 1046, 1449
FspBI CTAG 3 cut(s) 893, 1235, 1386
GlaI GCGC 1 cut(s) 784
GluI GCNGC 3 cut(s) 400, 1046, 1449
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 4 cut(s) 763, 989, 1063, 1135
HapII CCGG 2 cut(s) 403, 1317
HgaI GACGC 1 cut(s) 660
HhaI GCGC 1 cut(s) 785
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HinfI GANTC 5 cut(s) 559, 935, 1188, 1343, 1405
HpaII CCGG 2 cut(s) 403, 1317
HphI GGTGA 4 cut(s) 503, 736, 838, 1441
Hpy166II GTNNAC 3 cut(s) 511, 526, 1342
Hpy188I TCNGA 3 cut(s) 188, 718, 1187
Hpy188III TCNNGA 7 cut(s) 523, 556, 617, 802, 866, 1146, 1317
Hpy8I GTNNAC 3 cut(s) 511, 526, 1342
HpyAV CCTTC 5 cut(s) 84, 337, 636, 682, 977
HpyCH4IV ACGT 7 cut(s) 392, 415, 528, 593, 814, 855, 1213
HpyCH4V TGCA 8 cut(s) 8, 233, 359, 399, 772, 923, 952, 1451
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 707, 769
HpyF3I CTNAG 6 cut(s) 245, 931, 1058, 1246, 1367, 1414
HpySE526I ACGT 7 cut(s) 392, 415, 528, 593, 814, 855, 1213
HspAI GCGC 1 cut(s) 783
KflI GGGWCCC 1 cut(s) 808
Kpn2I TCCGGA 1 cut(s) 1316
KpnI GGTACC 1 cut(s) 97
Kzo9I GATC 2 cut(s) 451, 1492
LmnI GCTCC 4 cut(s) 428, 431, 474, 1389
LpnPI CCDG 7 cut(s) 416, 524, 551, 681, 1087, 1159, 1330
Lsp1109I GCAGC 2 cut(s) 411, 1435
LweI GCATC 7 cut(s) 242, 617, 635, 781, 891, 1125, 1401
MaeI CTAG 3 cut(s) 893, 1235, 1386
MaeII ACGT 7 cut(s) 392, 415, 528, 593, 814, 855, 1213
MaeIII GTNAC 6 cut(s) 248, 529, 724, 851, 1069, 1097
MalI GATC 2 cut(s) 453, 1494
MboI GATC 2 cut(s) 451, 1492
MboII GAAGA 5 cut(s) 421, 790, 1338, 1446, 1497
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 436, 991, 1197
MluCI AATT 9 cut(s) 160, 169, 227, 683, 1018, 1176, 1181, 1259, 1467
MlyI GAGTC 2 cut(s) 1337, 1414
MmeI TCCRAC 3 cut(s) 67, 1196, 1420
Mph1103I ATGCAT 1 cut(s) 774
MroI TCCGGA 1 cut(s) 1316
MroXI GAANNNNTTC 2 cut(s) 692, 1324
MseI TTAA 6 cut(s) 153, 270, 389, 1122, 1151, 1179
MslI CAYNNNNRTG 2 cut(s) 81, 418
MspI CCGG 2 cut(s) 403, 1317
MspR9I CCNGG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 365, 707, 769
NdeII GATC 2 cut(s) 451, 1492
NlaIV GGNNCC 6 cut(s) 95, 433, 809, 810, 988, 989
NmuCI GTSAC 1 cut(s) 724
NsiI ATGCAT 1 cut(s) 774
NspI RCATGY 4 cut(s) 12, 469, 732, 1098
PagI TCATGA 1 cut(s) 616
PctI GAATGC 1 cut(s) 652
PdmI GAANNNNTTC 2 cut(s) 692, 1324
PfeI GAWTC 3 cut(s) 559, 935, 1188
Pfl23II CGTACG 1 cut(s) 753
PflMI CCANNNNNTGG 2 cut(s) 442, 975
PkrI GCNGC 3 cut(s) 401, 1047, 1450
PleI GAGTC 2 cut(s) 1337, 1413
PmaCI CACGTG 1 cut(s) 815
PmlI CACGTG 1 cut(s) 815
PpsI GAGTC 2 cut(s) 1337, 1413
Ppu21I YACGTR 1 cut(s) 815
PpuMI RGGWCCY 1 cut(s) 808
PsiI TTATAA 1 cut(s) 27
Psp1406I AACGTT 2 cut(s) 593, 855
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 1 cut(s) 537
PspCI CACGTG 1 cut(s) 815
PspGI CCWGG 1 cut(s) 537
PspLI CGTACG 1 cut(s) 753
PspN4I GGNNCC 6 cut(s) 95, 433, 809, 810, 988, 989
PspOMI GGGCCC 1 cut(s) 987
PspPI GGNCC 6 cut(s) 762, 808, 987, 988, 1054, 1062
PspPPI RGGWCCY 1 cut(s) 808
PsrI GAACNNNNNNTAC 2 cut(s) 518, 550
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 7 cut(s) 95, 602, 755, 861, 945, 1233, 1479
RsaNI GTAC 7 cut(s) 94, 601, 754, 860, 944, 1232, 1478
RseI CAYNNNNRTG 2 cut(s) 81, 418
SaqAI TTAA 6 cut(s) 153, 270, 389, 1122, 1151, 1179
SatI GCNGC 3 cut(s) 400, 1046, 1449
Sau3AI GATC 2 cut(s) 451, 1492
Sau96I GGNCC 6 cut(s) 762, 808, 987, 988, 1054, 1062
SchI GAGTC 2 cut(s) 1337, 1414
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 3 cut(s) 436, 991, 1197
SfaNI GCATC 7 cut(s) 242, 617, 635, 781, 891, 1125, 1401
SinI GGWCC 2 cut(s) 808, 1054
SmiMI CAYNNNNRTG 2 cut(s) 81, 418
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
Sse9I AATT 9 cut(s) 160, 169, 227, 683, 1018, 1176, 1181, 1259, 1467
SsiI CCGC 2 cut(s) 1045, 1521
SspI AATATT 1 cut(s) 903
SspMI CTAG 3 cut(s) 893, 1235, 1386
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 3 cut(s) 584, 733, 1279
TaiI ACGT 7 cut(s) 395, 418, 531, 596, 817, 858, 1216
TasI AATT 9 cut(s) 160, 169, 227, 683, 1018, 1176, 1181, 1259, 1467
TatI WGTACW 1 cut(s) 859
TauI GCSGC 1 cut(s) 1048
TfiI GAWTC 3 cut(s) 559, 935, 1188
Tru1I TTAA 6 cut(s) 153, 270, 389, 1122, 1151, 1179
Tru9I TTAA 6 cut(s) 153, 270, 389, 1122, 1151, 1179
TscAI CASTG 1 cut(s) 1464
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 2 cut(s) 399, 1448
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 4 cut(s) 55, 356, 408, 1008
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 1 cut(s) 1464
Van91I CCANNNNNTGG 2 cut(s) 442, 975
VpaK11BI GGWCC 2 cut(s) 808, 1054
XapI RAATTY 5 cut(s) 160, 169, 227, 1018, 1181
XceI RCATGY 4 cut(s) 12, 469, 732, 1098
XcmI CCANNNNNNNNNTGG 1 cut(s) 1153
XmnI GAANNNNTTC 2 cut(s) 692, 1324
XspI CTAG 3 cut(s) 893, 1235, 1386
Zsp2I ATGCAT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.