pycom12654g00130

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012654
OG0013916
Physical Location & Seq
Reverse (-)
142827 .. 144873
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGGCACAGGCGCTCGAAACCATGTCCCTCTTTATTCCTTCTCCTCTCCGCTCTCCGATCGCATCATCTTTATTTACCCGAAAGACAAAAACAAGGTTGCATCAGCGCTTTACCCAACTCCCTGTAGTCCACTTCTCCTCCTGCTGTGGAGGAAGTGACGAGCTGAGGAAGAATTTTGATCCTAAAAGCTCAAAAATGGCTATATCGCCGATATGGGTATTTGGGTATGGCTCTCTTATCTGGAAGGCTGGTTTTAACTACGATGAGCGCCTCGTCGGCTTCATCAAAGGCTATCGCCGTGTCTTCCACCAAGGCAGTACGGACCACAGAGGGACGCCTGAATATCCAGGAAGAACAGTCACATTGGAACCTGCAGAAGGAGAAGTCTGTTGGGGAGTTGCCTACAAGATCTCAAACAAGGATGATCAAGAAGTTGCTATAACGTATTTAGAAGTGAGGGAGAAGCAGTATGACAAGAAGGCTTATCTTGATTTCTTCACCGATCCAATGGCGACATCTGCAGCTGTTTCAGGTGTAATGGTATACATCGCCTCTCCGGACAAGAAACATAATGTGAACTACTTGGGTCCTGCATCCACCGAAGAGATTGCCAAACAAATTGTTCAGGCTGAAGGCCCCTCGGGGCCTAATCGAGACTACCTTTTCCGCCTTGAAGAGGCACTTCTTCAACTAGGATGCAAAGACAAACATGTATTGGACCTTGCAAACGAAGTGAGGCGCATCCTGTCGGAGAGGGAGCTGACTTCAACATGA

Protein Analysis

258

Amino Acids

28.97

Weight (kDa)

8.25

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 71 - 247 1.9e-54 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 543
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 2 cut(s) 49, 667
AclWI GGATC 2 cut(s) 173, 497
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 1 cut(s) 319
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 377, 676
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 3 cut(s) 674, 689, 768
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 4 cut(s) 163, 189, 524, 760
AluI AGCT 4 cut(s) 163, 189, 524, 760
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 2 cut(s) 173, 497
Ama87I CYCGRG 1 cut(s) 640
Aor13HI TCCGGA 1 cut(s) 556
Aor51HI AGCGCT 1 cut(s) 107
AoxI GGCC 2 cut(s) 634, 644
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 172
AspLEI GCGC 4 cut(s) 13, 108, 270, 741
AspS9I GGNCC 5 cut(s) 322, 587, 635, 644, 718
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 640
AvaII GGWCC 3 cut(s) 322, 587, 718
BbsI GAAGAC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 1 cut(s) 348
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 3 cut(s) 123, 372, 519
BfoI RGCGCY 3 cut(s) 14, 109, 271
BfuAI ACCTGC 1 cut(s) 379
BglI GCCNNNNNGGC 1 cut(s) 276
BglII AGATCT 1 cut(s) 408
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 3 cut(s) 322, 587, 718
BmeT110I CYCGRG 1 cut(s) 640
BmgT120I GGNCC 5 cut(s) 322, 587, 635, 644, 718
BmiI GGNNCC 4 cut(s) 369, 588, 637, 645
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 5 cut(s) 71, 109, 602, 686, 750
BpiI GAAGAC 1 cut(s) 295
Bpu10I CCTNAGC 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 335
BsaJI CCNNGG 2 cut(s) 310, 639
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 452, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 676
BseAI TCCGGA 1 cut(s) 556
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 310, 639
BseGI GGATG 4 cut(s) 427, 593, 701, 741
BseLI CCNNNNNNNGG 2 cut(s) 377, 676
BseMII CTCAG 1 cut(s) 155
BseRI GAGGAG 2 cut(s) 33, 127
BseXI GCAGC 1 cut(s) 533
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 636, 646
BsiEI CGRYCG 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 640
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 2 cut(s) 10, 346
BslI CCNNNNNNNGG 2 cut(s) 377, 676
BsmAI GTCTC 1 cut(s) 648
BsmFI GGGAC 2 cut(s) 10, 346
BsnI GGCC 2 cut(s) 636, 646
BsoBI CYCGRG 1 cut(s) 640
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 5 cut(s) 57, 178, 408, 424, 502
BspACI CCGC 2 cut(s) 49, 667
BspANI GGCC 2 cut(s) 636, 646
BspCNI CTCAG 1 cut(s) 156
BspEI TCCGGA 1 cut(s) 556
BspLI GGNNCC 4 cut(s) 369, 588, 637, 645
BspMAI CTGCAG 2 cut(s) 376, 523
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 2 cut(s) 173, 497
BsrBI CCGCTC 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 310, 639
BssMI GATC 5 cut(s) 57, 178, 408, 424, 502
BssNAI GTATAC 1 cut(s) 544
BssNI GRCGYC 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 310
Bst1107I GTATAC 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 2 cut(s) 597, 669
BstACI GRCGYC 1 cut(s) 335
BstDEI CTNAG 1 cut(s) 164
BstENI CCTNNNNNAGG 2 cut(s) 375, 674
BstF5I GGATG 4 cut(s) 427, 593, 701, 741
BstH2I RGCGCY 3 cut(s) 14, 109, 271
BstHHI GCGC 4 cut(s) 13, 108, 270, 741
BstKTI GATC 5 cut(s) 60, 181, 411, 427, 505
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 5 cut(s) 57, 178, 408, 424, 502
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 276, 518
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 3 cut(s) 123, 372, 519
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BstZ17I GTATAC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 636, 646
BtgZI GCGATG 1 cut(s) 532
BtsCI GGATG 4 cut(s) 427, 593, 701, 741
BveI ACCTGC 1 cut(s) 379
CfoI GCGC 4 cut(s) 13, 108, 270, 741
Cfr13I GGNCC 5 cut(s) 322, 587, 635, 644, 718
CseI GACGC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 318
CviAII CATG 3 cut(s) 22, 710, 771
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 1 cut(s) 164
DpnI GATC 5 cut(s) 59, 180, 410, 426, 504
DpnII GATC 5 cut(s) 57, 178, 408, 424, 502
Eam1104I CTCTTC 2 cut(s) 597, 669
EarI CTCTTC 2 cut(s) 597, 669
EciI GGCGGA 1 cut(s) 656
Eco130I CCWWGG 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 322, 587, 718
Eco47III AGCGCT 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 640
EcoNI CCTNNNNNAGG 2 cut(s) 375, 674
EcoO109I RGGNCCY 3 cut(s) 587, 635, 644
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 3 cut(s) 25, 713, 774
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 2 cut(s) 10, 346
FatI CATG 3 cut(s) 21, 709, 770
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 543
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 4 cut(s) 434, 580, 708, 728
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 1 cut(s) 692
GlaI GCGC 4 cut(s) 12, 107, 269, 740
GluI GCNGC 1 cut(s) 522
HaeII RGCGCY 3 cut(s) 14, 109, 271
HaeIII GGCC 2 cut(s) 636, 646
HapII CCGG 1 cut(s) 557
HgaI GACGC 1 cut(s) 343
HhaI GCGC 4 cut(s) 13, 108, 270, 741
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 3 cut(s) 25, 713, 774
Hin6I GCGC 4 cut(s) 11, 106, 268, 739
HinP1I GCGC 4 cut(s) 11, 106, 268, 739
HpaII CCGG 1 cut(s) 557
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 3 cut(s) 130, 544, 577
Hpy188I TCNGA 2 cut(s) 57, 751
Hpy188III TCNNGA 5 cut(s) 241, 428, 488, 557, 653
Hpy8I GTNNAC 3 cut(s) 130, 544, 577
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 5 cut(s) 48, 238, 371, 472, 626
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 6 cut(s) 100, 374, 521, 593, 699, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 518
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 443
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 3 cut(s) 25, 713, 774
HspAI GCGC 4 cut(s) 11, 106, 268, 739
Kpn2I TCCGGA 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 5 cut(s) 57, 178, 408, 424, 502
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 5 cut(s) 71, 109, 602, 686, 750
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 155, 358
MalI GATC 5 cut(s) 59, 180, 410, 426, 504
MbiI CCGCTC 1 cut(s) 51
MboI GATC 5 cut(s) 57, 178, 408, 424, 502
MboII GAAGA 7 cut(s) 181, 295, 363, 487, 614, 677, 686
MflI RGATCY 1 cut(s) 408
MluCI AATT 2 cut(s) 172, 618
MmeI TCCRAC 1 cut(s) 729
MroI TCCGGA 1 cut(s) 556
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 2 cut(s) 276, 518
NdeII GATC 5 cut(s) 57, 178, 408, 424, 502
NlaIII CATG 3 cut(s) 25, 713, 774
NlaIV GGNNCC 4 cut(s) 369, 588, 637, 645
NmuCI GTSAC 2 cut(s) 155, 358
NspI RCATGY 1 cut(s) 713
PciI ACATGT 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 346
PkrI GCNGC 1 cut(s) 523
Ple19I CGATCG 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 587
PscI ACATGT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 4 cut(s) 369, 588, 637, 645
PspPI GGNCC 5 cut(s) 322, 587, 635, 644, 718
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 2 cut(s) 376, 523
PsuI RGATCY 1 cut(s) 408
PvuI CGATCG 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 5 cut(s) 57, 178, 408, 424, 502
Sau96I GGNCC 5 cut(s) 322, 587, 635, 644, 718
ScrFI CCNGG 1 cut(s) 348
SfaNI GCATC 5 cut(s) 71, 109, 602, 686, 750
SfcI CTRYAG 3 cut(s) 123, 372, 519
SinI GGWCC 3 cut(s) 322, 587, 718
Sse9I AATT 2 cut(s) 172, 618
SsiI CCGC 2 cut(s) 49, 667
SspMI CTAG 1 cut(s) 692
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 446
TaqI TCGA 2 cut(s) 15, 652
TasI AATT 2 cut(s) 172, 618
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseFI GTSAC 2 cut(s) 155, 358
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 155, 358
TspDTI ATGAA 1 cut(s) 271
TspGWI ACGGA 1 cut(s) 335
VpaK11BI GGWCC 3 cut(s) 322, 587, 718
XagI CCTNNNNNAGG 2 cut(s) 375, 674
XapI RAATTY 1 cut(s) 172
XceI RCATGY 1 cut(s) 713
XmiI GTMKAC 1 cut(s) 543
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.