pycom12g00840

Domain of unknown function (DUF4602)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007194
Physical Location & Seq
Reverse (-)
725124 .. 727712
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGAATATGAAATGGGTTGCCCAGTTGAAATACCTAGCCCACCAAGCCCACCTAGCAGCCCATCGTCGAGTCCCGAACGCGAGCACGCAGCCTCCTCATTCTTCAGCACTAAGTCCTCCCTGTAGCTCGAGCCGCCGCTCCACCGCCATTCTGCAGCACTCCTCCACCCAGCGCCGCCCTCTGTTACTAGGGCTTTTGCAATACTTTGTTCCCAATTTGTTCCAACAAATAGGAAAAATGCAGAAGAATAAGGGAAGACAAAGAGGAGGGGGACCATTTACGAGACGAACGGATAACAAGGAAGAAAACTCAGACACCCACTTCCAAAAAATGGACTTGAGGAACATTGCTAAAGAGATTGAGCTTTTGGGTACTTCTAATATGACTTGGAAGGAGAGAAAGGAGTTGGAAAATAGGAGGGTGGTTTCTCTAGGCGGAAAGCCTCAAAAGAACCGGCGACTACCTCTAAGTGTAGCAAAGCTGGTCATGAAGAATCAAAAGAAAAGGGAGCAAAAACTGCAAGAACAGAACATGGTCCTTGGACGTTTTGGTGGGAATTCAAGTGGCAGTTCTAAGAGATCCACAGAGAGGAAGAGGCCCGAGAACAGGGGTTTGAAAGGAAGCGAAGGACACTTTAAAAACGGTGTACTTGATGTGAAGCATTTGCTACGTCCTACTTCATCTGGAGACACATATGCTGGTCCGAGTATGTTCAGAGGGAAAACTAAGGGGAAGCGAAAGAACAACACAGGTAAGAAGATCGGTGGTATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4602

Protein Analysis

259

Amino Acids

29.13

Weight (kDa)

11.24

Isoelectric Point (pI)

59.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSAF1 PF15375 83 - 198 3.5e-15 40S small subunit processome assembly factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 80
AciI CCGC 5 cut(s) 133, 136, 144, 175, 435
AclWI GGATC 1 cut(s) 573
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 2 cut(s) 373, 648
AfiI CCNNNNNNNGG 3 cut(s) 331, 588, 606
AgsI TTSAA 3 cut(s) 28, 561, 616
AluBI AGCT 3 cut(s) 126, 364, 481
AluI AGCT 3 cut(s) 126, 364, 481
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 2 cut(s) 277, 681
AlwI GGATC 1 cut(s) 573
Ama87I CYCGRG 2 cut(s) 127, 599
AoxI GGCC 1 cut(s) 596
ApeKI GCWGC 3 cut(s) 56, 88, 154
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 4 cut(s) 272, 535, 597, 701
AvaI CYCGRG 2 cut(s) 127, 599
AvaII GGWCC 3 cut(s) 272, 535, 701
BbsI GAAGAC 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 68, 100, 166
BccI CCATC 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 277, 681
BfaI CTAG 5 cut(s) 35, 53, 188, 431, 775
BfmI CTRYAG 2 cut(s) 121, 152
BfoI RGCGCY 1 cut(s) 175
BisI GCNGC 6 cut(s) 57, 89, 133, 136, 155, 175
BlsI GCNGC 6 cut(s) 58, 90, 134, 137, 156, 176
Bme18I GGWCC 3 cut(s) 272, 535, 701
BmeT110I CYCGRG 2 cut(s) 127, 599
BmgT120I GGNCC 4 cut(s) 272, 535, 597, 701
BmiI GGNNCC 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 16
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 588, 606
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 538
BseLI CCNNNNNNNGG 3 cut(s) 331, 588, 606
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 3 cut(s) 84, 151, 279
BseXI GCAGC 3 cut(s) 68, 100, 166
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 86
BsiHKCI CYCGRG 2 cut(s) 127, 599
BsiSI CCGG 1 cut(s) 454
BslFI GGGAC 2 cut(s) 56, 285
BslI CCNNNNNNNGG 3 cut(s) 331, 588, 606
BsmAI GTCTC 2 cut(s) 277, 681
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 2 cut(s) 56, 285
BsnI GGCC 1 cut(s) 598
BsoBI CYCGRG 2 cut(s) 127, 599
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 578, 759
BspACI CCGC 5 cut(s) 133, 136, 144, 175, 435
BspANI GGCC 1 cut(s) 598
BspCNI CTCAG 1 cut(s) 323
BspFNI CGCG 1 cut(s) 80
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 156
BspPI GGATC 1 cut(s) 573
BsrBI CCGCTC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 453
BsrI ACTGG 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 1 cut(s) 538
BssMI GATC 2 cut(s) 578, 759
BssT1I CCWWGG 1 cut(s) 538
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 517
BstC8I GCNNGC 2 cut(s) 82, 86
BstDEI CTNAG 5 cut(s) 110, 310, 467, 573, 726
BstFNI CGCG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 2 cut(s) 581, 762
BstMAI GTCTC 2 cut(s) 277, 681
BstMBI GATC 2 cut(s) 578, 759
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 132, 517
BstSFI CTRYAG 2 cut(s) 121, 152
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 68, 100, 166
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 578
BstYI RGATCY 1 cut(s) 578
BsuRI GGCC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 82, 86
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 4 cut(s) 272, 535, 597, 701
Csp6I GTAC 2 cut(s) 372, 647
CviAII CATG 2 cut(s) 487, 532
CviQI GTAC 2 cut(s) 372, 647
DdeI CTNAG 5 cut(s) 110, 310, 467, 573, 726
DpnI GATC 2 cut(s) 580, 761
DpnII GATC 2 cut(s) 578, 759
DraI TTTAAA 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
EciI GGCGGA 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 538
Eco47I GGWCC 3 cut(s) 272, 535, 701
Eco57I CTGAAG 1 cut(s) 87
Eco88I CYCGRG 2 cut(s) 127, 599
EcoRI GAATTC 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 538
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 2 cut(s) 490, 535
FaiI YATR 8 cut(s) 8, 383, 488, 533, 694, 696, 710, 770
FaqI GGGAC 2 cut(s) 56, 285
FatI CATG 2 cut(s) 486, 531
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 6 cut(s) 57, 89, 133, 136, 155, 175
Fsp4HI GCNGC 6 cut(s) 57, 89, 133, 136, 155, 175
FspBI CTAG 5 cut(s) 35, 53, 188, 431, 775
GlaI GCGC 1 cut(s) 173
GluI GCNGC 6 cut(s) 57, 89, 133, 136, 155, 175
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 1 cut(s) 598
HapII CCGG 1 cut(s) 454
HhaI GCGC 1 cut(s) 174
Hin1II CATG 2 cut(s) 490, 535
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 2 cut(s) 69, 493
HpaII CCGG 1 cut(s) 454
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 3 cut(s) 313, 705, 716
Hpy188III TCNNGA 3 cut(s) 73, 487, 684
Hpy8I GTNNAC 1 cut(s) 647
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 2 cut(s) 385, 620
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 2 cut(s) 544, 670
HpyCH4V TGCA 4 cut(s) 154, 199, 241, 520
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 132, 517
HpyF3I CTNAG 5 cut(s) 110, 310, 467, 573, 726
HpySE526I ACGT 2 cut(s) 544, 670
Hsp92II CATG 2 cut(s) 490, 535
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 578, 759
LmnI GCTCC 2 cut(s) 143, 508
LpnPI CCDG 9 cut(s) 35, 133, 182, 467, 467, 592, 669, 684, 735
Lsp1109I GCAGC 3 cut(s) 68, 100, 166
MaeI CTAG 5 cut(s) 35, 53, 188, 431, 775
MaeII ACGT 2 cut(s) 544, 670
MaeIII GTNAC 1 cut(s) 183
MalI GATC 2 cut(s) 580, 761
MbiI CCGCTC 1 cut(s) 138
MboI GATC 2 cut(s) 578, 759
MboII GAAGA 7 cut(s) 93, 256, 267, 314, 502, 604, 769
MflI RGATCY 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 2 cut(s) 214, 556
MlyI GAGTC 1 cut(s) 78
MmeI TCCRAC 2 cut(s) 247, 387
MseI TTAA 1 cut(s) 636
MspI CCGG 1 cut(s) 454
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 132, 517
NdeI CATATG 1 cut(s) 694
NdeII GATC 2 cut(s) 578, 759
NlaIII CATG 2 cut(s) 490, 535
NlaIV GGNNCC 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 127
PagI TCATGA 1 cut(s) 486
PfeI GAWTC 1 cut(s) 493
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 6 cut(s) 58, 90, 134, 137, 156, 176
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 4 cut(s) 272, 535, 597, 701
PspXI VCTCGAGB 1 cut(s) 127
PstI CTGCAG 1 cut(s) 156
PsuI RGATCY 1 cut(s) 578
RsaI GTAC 2 cut(s) 373, 648
RsaNI GTAC 2 cut(s) 372, 647
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 6 cut(s) 57, 89, 133, 136, 155, 175
Sau3AI GATC 2 cut(s) 578, 759
Sau96I GGNCC 4 cut(s) 272, 535, 597, 701
SchI GAGTC 1 cut(s) 78
SduI GDGCHC 1 cut(s) 86
SetI ASST 9 cut(s) 36, 54, 128, 366, 466, 483, 547, 673, 754
SfcI CTRYAG 2 cut(s) 121, 152
Sfr274I CTCGAG 1 cut(s) 127
SinI GGWCC 3 cut(s) 272, 535, 701
SlaI CTCGAG 1 cut(s) 127
SmlI CTYRAG 2 cut(s) 127, 337
SmoI CTYRAG 2 cut(s) 127, 337
Sse9I AATT 2 cut(s) 214, 556
SsiI CCGC 5 cut(s) 133, 136, 144, 175, 435
SspMI CTAG 5 cut(s) 35, 53, 188, 431, 775
StyI CCWWGG 1 cut(s) 538
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 2 cut(s) 547, 673
TaqI TCGA 2 cut(s) 67, 128
TasI AATT 2 cut(s) 214, 556
TatI WGTACW 1 cut(s) 646
TauI GCSGC 3 cut(s) 135, 138, 177
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TseI GCWGC 3 cut(s) 56, 88, 154
TspDTI ATGAA 4 cut(s) 17, 23, 503, 669
TspGWI ACGGA 1 cut(s) 305
Van91I CCANNNNNTGG 1 cut(s) 331
VpaK11BI GGWCC 3 cut(s) 272, 535, 701
XapI RAATTY 1 cut(s) 556
XhoI CTCGAG 1 cut(s) 127
XspI CTAG 5 cut(s) 35, 53, 188, 431, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.