pycom12g00930

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004978
Physical Location & Seq
Reverse (-)
806480 .. 808343
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1578 bp
ATGATTAGAATAACAGATTCCTTGCAGTTTACCTCTTTCCGACATCATTTTCGTCTGTCTTCGTTGATCTTGATACATCCGGAATTTTCGGTGCATCTGATTTTGTTGAAACTTGGAGGCAAAGAAAGTGGTAGAAACCTTCCTAGAACTTCTGTACGAGAAAGGACAGTTGATTTCGGAATTTTGAAGCCATTAAGATATGTGAATCGAGTTATGGCAAGTTTAGAGACTACCAAGAACATCCACGTACAAGTTCAAGAAGCAGAACTCAGACCTTCCTCCATAAACCTAATCAAGAAGCAAGAACCTAGCAACTTTCCACCTGAAACAACCACAGCCGGACAATTTCTCAGGAGGCTCTTTAAGCTTCTTTTTTACTTGCACTTGATCCTCATCACGATCTTCATAATCTTCCTTACGCTCTATGTTCTCATCTTCAACACTCGCACTCACCATTTCCGACCCCTTGAGTATTACCCTCCATTGCTCACTGCAATTGGGTGCTCTGCAATTTTTACTTTCATATGGCAGTGGATCACTAGCTCAAGCCCTTCCAAAGCCTTCAAGGCAGCGTTCTGGCTTAGCCCTTTGCTAACATGTGCGGTCGGTGTACTACTTATAGACATAAGTTCTCCTGCGAGTTTGGCTGCAGGTATGGTTGCAATCGTTTGTGCCGTCGTTCAGTCTCTGTATGCCTGTTGGGTCAGTCCCCGTTTTGACTACGCAATTCGAGTTTTATCAGTTTCCACTGCCTTTCCTCCTCCGAAGACTACAACATTAGTCATCCGATCAATTTCCGTCAGCATTCTTTACTCGTGTCTCTTGGTGTCCGGCATTGGAAGAGCCACTGCTATCAGGAGTAGTTGGAAAGCCCTGTTCATCGCACTAATCTTGCTAAGCATGGCATGGACGATGCAAGTAATCAAGAACACGCTGCTAGTTACCGTCTCGCGAATCAAGTATATGAACTTCACATTTGGAACAGAGATTGACACGAATGAGGCTTTTCGTGACACCATTAAGCACTTGACTGGGAGCATCTGCATCGGCTCCATCTTGGTCCCGGTTCTTGGGGTTGTCCGTGGTTCAGCCCGAGCCATGAAACTGACTGCAGGGGATACAGACGAGTTTCTGTTTTCGTGTGCCAACTGCTATTCAGGAATGGCTTCGACGCTGGTGATATATGGGAATCGCTGGGGTTTTGTGCACGTAGGAGCGTACAATAAGGGGTTTGTGCAGGCATCGTCGGATACTTGGGAGATGTTCGGGAACGAAGGACTGAAAGAATTAATCGACCGGGATCTCACCGGATCGTTCTGTTTTCTTTCTGGGGTGGCAGCAGGTGCAATCTGCAGCCTAGTGAGCGGGACTTGGGCTCTTATAATTCACAAGAGCTATGCTACAGAAGTTTCAATATACGCATTCTTCATCGGATACTTCGTGTGTAGGGTTGCTATGGCATGGCCACAAGCATGCGTCTCGTCTTACTACGTGGCGTATGCAGAAGATCCGCGGAGCATTAGATTTGATTCGACCATACCTGCTCGTATTGAAGAGCTCCGGAGATTTCAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

526

Amino Acids

58.64

Weight (kDa)

9.41

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 205 - 504 7.5e-26 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1382
AarI CACCTGC 1 cut(s) 1331
Acc36I ACCTGC 3 cut(s) 641, 1331, 1549
AccBSI CCGCTC 1 cut(s) 1365
AccII CGCG 2 cut(s) 952, 1513
AccIII TCCGGA 2 cut(s) 79, 1560
AciI CCGC 4 cut(s) 602, 1365, 1511, 1513
AclWI GGATC 5 cut(s) 382, 542, 1308, 1318, 1502
AcoI YGGCCR 1 cut(s) 1463
AcsI RAATTY 2 cut(s) 83, 180
AfaI GTAC 4 cut(s) 156, 249, 612, 1220
AfiI CCNNNNNNNGG 1 cut(s) 1070
AflIII ACRYGT 1 cut(s) 596
AgsI TTSAA 7 cut(s) 109, 187, 257, 439, 565, 1413, 1553
AluBI AGCT 4 cut(s) 367, 543, 1395, 1558
AluI AGCT 4 cut(s) 367, 543, 1395, 1558
Alw21I GWGCWC 3 cut(s) 506, 1209, 1560
Alw26I GTCTC 5 cut(s) 221, 690, 824, 952, 1483
Alw44I GTGCAC 1 cut(s) 1205
AlwI GGATC 5 cut(s) 382, 542, 1308, 1318, 1502
AlwNI CAGNNNCTG 1 cut(s) 688
Ama87I CYCGRG 1 cut(s) 1092
Aor13HI TCCGGA 2 cut(s) 79, 1560
AoxI GGCC 1 cut(s) 1463
ApaLI GTGCAC 1 cut(s) 1205
ApeKI GCWGC 5 cut(s) 569, 647, 934, 1337, 1353
ApoI RAATTY 2 cut(s) 83, 180
ArsI GACNNNNNNTTYG 2 cut(s) 33, 65
AseI ATTAAT 1 cut(s) 1289
Asp700I GAANNNNTTC 1 cut(s) 1165
AspS9I GGNCC 1 cut(s) 1060
AsuC2I CCSGG 2 cut(s) 1064, 1298
AsuHPI GGTGA 3 cut(s) 443, 1189, 1297
AvaI CYCGRG 1 cut(s) 1092
AvaII GGWCC 1 cut(s) 1060
BaeGI GKGCMC 1 cut(s) 1209
BalI TGGCCA 1 cut(s) 1465
BanII GRGCYC 2 cut(s) 1378, 1560
BarI GAAGNNNNNNTAC 2 cut(s) 953, 985
BauI CACGAG 1 cut(s) 814
BbsI GAAGAC 2 cut(s) 51, 773
Bbv12I GWGCWC 3 cut(s) 506, 1209, 1560
BbvI GCAGC 5 cut(s) 581, 634, 921, 1349, 1365
BccI CCATC 1 cut(s) 1061
BceAI ACGGC 1 cut(s) 659
BcgI CGANNNNNNTGC 4 cut(s) 1027, 1061, 1461, 1495
BciVI GTATCC 3 cut(s) 1111, 1243, 1427
BcnI CCSGG 2 cut(s) 1064, 1298
BcoDI GTCTC 5 cut(s) 221, 690, 824, 952, 1483
BfaI CTAG 5 cut(s) 144, 309, 540, 938, 1358
BfmI CTRYAG 4 cut(s) 648, 1110, 1351, 1401
BfuAI ACCTGC 3 cut(s) 641, 1331, 1549
BfuI GTATCC 3 cut(s) 1111, 1243, 1427
BglI GCCNNNNNGGC 1 cut(s) 566
BisI GCNGC 5 cut(s) 570, 648, 935, 1338, 1354
BlpI GCTNAGC 2 cut(s) 581, 896
BlsI GCNGC 5 cut(s) 571, 649, 936, 1339, 1355
Bme1390I CCNGG 2 cut(s) 1064, 1298
Bme18I GGWCC 1 cut(s) 1060
BmeT110I CYCGRG 1 cut(s) 1092
BmgT120I GGNCC 1 cut(s) 1060
BmiI GGNNCC 2 cut(s) 1051, 1062
BmrFI CCNGG 2 cut(s) 1064, 1298
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 5 cut(s) 103, 903, 1047, 1053, 1250
BmuI ACTGGG 1 cut(s) 1041
BpiI GAAGAC 2 cut(s) 51, 773
Bpu1102I GCTNAGC 2 cut(s) 581, 896
BpuEI CTTGAG 2 cut(s) 488, 529
BpuMI CCSGG 2 cut(s) 1064, 1298
BsaAI YACGTR 3 cut(s) 247, 1210, 1492
BsaBI GATNNNNATC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 1081, 1511
BsaWI WCCGGW 3 cut(s) 79, 1307, 1560
Bsc4I CCNNNNNNNGG 1 cut(s) 1070
Bse1I ACTGG 1 cut(s) 1036
Bse3DI GCAATG 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 392
BseAI TCCGGA 2 cut(s) 79, 1560
BseDI CCNNGG 2 cut(s) 1081, 1511
BseGI GGATG 3 cut(s) 76, 240, 783
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 1 cut(s) 1070
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 2 cut(s) 283, 364
BseNI ACTGG 1 cut(s) 1036
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 1209
BseXI GCAGC 5 cut(s) 581, 634, 921, 1349, 1365
BseYI CCCAGC 1 cut(s) 1194
BsgI GTGCAG 1 cut(s) 1256
Bsh1236I CGCG 2 cut(s) 952, 1513
Bsh1285I CGRYCG 2 cut(s) 606, 1297
BshFI GGCC 1 cut(s) 1465
BsiEI CGRYCG 2 cut(s) 606, 1297
BsiHKAI GWGCWC 3 cut(s) 506, 1209, 1560
BsiHKCI CYCGRG 1 cut(s) 1092
BsiSI CCGG 7 cut(s) 80, 339, 831, 1064, 1297, 1308, 1561
BslFI GGGAC 3 cut(s) 693, 1046, 1381
BslI CCNNNNNNNGG 1 cut(s) 1070
BsmAI GTCTC 5 cut(s) 221, 690, 824, 952, 1483
BsmBI CGTCTC 2 cut(s) 952, 1483
BsmFI GGGAC 3 cut(s) 693, 1046, 1381
BsmI GAATGC 2 cut(s) 804, 1421
BsnI GGCC 1 cut(s) 1465
BsoBI CYCGRG 1 cut(s) 1092
Bsp1286I GDGCHC 4 cut(s) 506, 1209, 1378, 1560
Bsp13I TCCGGA 2 cut(s) 79, 1560
Bsp143I GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
Bsp1720I GCTNAGC 2 cut(s) 581, 896
Bsp68I TCGCGA 1 cut(s) 952
BspACI CCGC 4 cut(s) 602, 1365, 1511, 1513
BspANI GGCC 1 cut(s) 1465
BspCNI CTCAG 2 cut(s) 282, 363
BspEI TCCGGA 2 cut(s) 79, 1560
BspFNI CGCG 2 cut(s) 952, 1513
BspLI GGNNCC 2 cut(s) 1051, 1062
BspMAI CTGCAG 3 cut(s) 652, 1114, 1355
BspMI ACCTGC 3 cut(s) 641, 1331, 1549
BspPI GGATC 5 cut(s) 382, 542, 1308, 1318, 1502
BspQI GCTCTTC 2 cut(s) 835, 1548
BsrBI CCGCTC 1 cut(s) 1365
BsrDI GCAATG 1 cut(s) 482
BsrI ACTGG 1 cut(s) 1036
BssECI CCNNGG 2 cut(s) 1081, 1511
BssMI GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
BssSI CACGAG 1 cut(s) 814
Bst2BI CACGAG 1 cut(s) 814
Bst4CI ACNGT 2 cut(s) 169, 946
Bst6I CTCTTC 2 cut(s) 835, 1548
BstAPI GCANNNNNTGC 1 cut(s) 1343
BstBAI YACGTR 3 cut(s) 247, 1210, 1492
BstC8I GCNNGC 2 cut(s) 1239, 1474
BstDEI CTNAG 4 cut(s) 269, 350, 581, 896
BstDSI CCRYGG 2 cut(s) 1081, 1511
BstF5I GGATG 3 cut(s) 76, 240, 783
BstFNI CGCG 2 cut(s) 952, 1513
BstKTI GATC 8 cut(s) 69, 390, 402, 537, 791, 1303, 1313, 1510
BstMAI GTCTC 5 cut(s) 221, 690, 824, 952, 1483
BstMBI GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
BstMCI CGRYCG 2 cut(s) 606, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 364, 566, 644, 1343, 1362
BstNSI RCATGY 2 cut(s) 600, 1476
BstSCI CCNGG 2 cut(s) 1062, 1296
BstSFI CTRYAG 4 cut(s) 648, 1110, 1351, 1401
BstSLI GKGCMC 1 cut(s) 1209
BstUI CGCG 2 cut(s) 952, 1513
BstV1I GCAGC 5 cut(s) 581, 634, 921, 1349, 1365
BstV2I GAAGAC 2 cut(s) 51, 773
BstX2I RGATCY 2 cut(s) 1300, 1507
BstYI RGATCY 2 cut(s) 1300, 1507
BsuI GTATCC 3 cut(s) 1111, 1243, 1427
BsuRI GGCC 1 cut(s) 1465
BtgI CCRYGG 2 cut(s) 1081, 1511
BtgZI GCGATG 1 cut(s) 865
BtsCI GGATG 3 cut(s) 76, 240, 783
BtsI GCAGTG 4 cut(s) 489, 536, 747, 846
BtsIMutI CAGTG 4 cut(s) 489, 536, 747, 846
BtuMI TCGCGA 1 cut(s) 952
BveI ACCTGC 3 cut(s) 641, 1331, 1549
Cac8I GCNNGC 2 cut(s) 1239, 1474
CaiI CAGNNNCTG 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 1060
Cfr42I CCGCGG 1 cut(s) 1514
CseI GACGC 2 cut(s) 1180, 1465
Csp6I GTAC 4 cut(s) 155, 248, 611, 1219
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 6 cut(s) 597, 901, 906, 1099, 1461, 1473
CviQI GTAC 4 cut(s) 155, 248, 611, 1219
DdeI CTNAG 4 cut(s) 269, 350, 581, 896
DpnI GATC 8 cut(s) 68, 389, 401, 536, 790, 1302, 1312, 1509
DpnII GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
EaeI YGGCCR 1 cut(s) 1463
Eam1104I CTCTTC 2 cut(s) 835, 1548
EarI CTCTTC 2 cut(s) 835, 1548
Ecl136II GAGCTC 1 cut(s) 1558
Eco24I GRGCYC 2 cut(s) 1378, 1560
Eco47I GGWCC 1 cut(s) 1060
Eco53kI GAGCTC 1 cut(s) 1558
Eco88I CYCGRG 1 cut(s) 1092
EcoICRI GAGCTC 1 cut(s) 1558
EcoT38I GRGCYC 2 cut(s) 1378, 1560
Esp3I CGTCTC 2 cut(s) 952, 1483
FaeI CATG 6 cut(s) 600, 904, 909, 1102, 1464, 1476
FaqI GGGAC 3 cut(s) 693, 1046, 1381
FatI CATG 6 cut(s) 596, 900, 905, 1098, 1460, 1472
FauI CCCGC 1 cut(s) 1358
FauNDI CATATG 1 cut(s) 524
Fnu4HI GCNGC 5 cut(s) 570, 648, 935, 1338, 1354
FokI GGATG 3 cut(s) 63, 227, 770
FriOI GRGCYC 2 cut(s) 1378, 1560
Fsp4HI GCNGC 5 cut(s) 570, 648, 935, 1338, 1354
FspBI CTAG 5 cut(s) 144, 309, 540, 938, 1358
GluI GCNGC 5 cut(s) 570, 648, 935, 1338, 1354
GsaI CCCAGC 1 cut(s) 1198
HaeIII GGCC 1 cut(s) 1465
HapII CCGG 7 cut(s) 80, 339, 831, 1064, 1297, 1308, 1561
HgaI GACGC 2 cut(s) 1180, 1465
Hin1II CATG 6 cut(s) 600, 904, 909, 1102, 1464, 1476
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 5 cut(s) 17, 205, 954, 1189, 1529
HpaII CCGG 7 cut(s) 80, 339, 831, 1064, 1297, 1308, 1561
HphI GGTGA 3 cut(s) 443, 1189, 1297
Hpy166II GTNNAC 3 cut(s) 30, 611, 1207
Hpy188I TCNGA 9 cut(s) 41, 99, 179, 272, 461, 765, 788, 1249, 1433
Hpy8I GTNNAC 3 cut(s) 30, 611, 1207
Hpy99I CGWCG 3 cut(s) 680, 1174, 1249
HpyAV CCTTC 5 cut(s) 149, 285, 561, 571, 1268
HpyCH4III ACNGT 2 cut(s) 169, 946
HpyCH4IV ACGT 3 cut(s) 246, 1209, 1491
HpyF10VI GCNNNNNNNGC 5 cut(s) 364, 566, 644, 1343, 1362
HpyF3I CTNAG 4 cut(s) 269, 350, 581, 896
HpySE526I ACGT 3 cut(s) 246, 1209, 1491
Hsp92II CATG 6 cut(s) 600, 904, 909, 1102, 1464, 1476
Kpn2I TCCGGA 2 cut(s) 79, 1560
KspI CCGCGG 1 cut(s) 1514
Kzo9I GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
LguI GCTCTTC 2 cut(s) 835, 1548
LmnI GCTCC 5 cut(s) 1035, 1055, 1214, 1515, 1563
Lsp1109I GCAGC 5 cut(s) 581, 634, 921, 1349, 1365
LweI GCATC 5 cut(s) 103, 903, 1047, 1053, 1250
MaeI CTAG 5 cut(s) 144, 309, 540, 938, 1358
MaeII ACGT 3 cut(s) 246, 1209, 1491
MaeIII GTNAC 2 cut(s) 940, 1010
MalI GATC 8 cut(s) 68, 389, 401, 536, 790, 1302, 1312, 1509
MbiI CCGCTC 1 cut(s) 1365
MboI GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
MboII GAAGA 9 cut(s) 51, 394, 403, 427, 778, 852, 1417, 1517, 1565
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 2 cut(s) 1300, 1507
MhlI GDGCHC 4 cut(s) 506, 1209, 1378, 1560
MlsI TGGCCA 1 cut(s) 1465
MluCI AATT 9 cut(s) 83, 180, 344, 495, 510, 726, 792, 1286, 1383
MluNI TGGCCA 1 cut(s) 1465
MmeI TCCRAC 4 cut(s) 64, 484, 845, 1227
MnlI CCTC 9 cut(s) 43, 110, 289, 348, 401, 489, 768, 771, 994
Mox20I TGGCCA 1 cut(s) 1465
MroI TCCGGA 2 cut(s) 79, 1560
MroXI GAANNNNTTC 1 cut(s) 1165
MscI TGGCCA 1 cut(s) 1465
MseI TTAA 4 cut(s) 194, 363, 1020, 1289
MslI CAYNNNNRTG 1 cut(s) 1471
Msp20I TGGCCA 1 cut(s) 1465
MspA1I CMGCKG 1 cut(s) 1513
MspI CCGG 7 cut(s) 80, 339, 831, 1064, 1297, 1308, 1561
MspR9I CCNGG 2 cut(s) 1064, 1298
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 804, 1421
MvnI CGCG 2 cut(s) 952, 1513
MwoI GCNNNNNNNGC 5 cut(s) 364, 566, 644, 1343, 1362
NciI CCSGG 2 cut(s) 1064, 1298
NdeI CATATG 1 cut(s) 524
NdeII GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
NlaIII CATG 6 cut(s) 600, 904, 909, 1102, 1464, 1476
NlaIV GGNNCC 2 cut(s) 1051, 1062
NmuCI GTSAC 1 cut(s) 1010
NruI TCGCGA 1 cut(s) 952
NspI RCATGY 2 cut(s) 600, 1476
PaeI GCATGC 1 cut(s) 1476
PaqCI CACCTGC 1 cut(s) 1331
PciI ACATGT 1 cut(s) 596
PciSI GCTCTTC 2 cut(s) 835, 1548
PcsI WCGNNNNNNNCGW 2 cut(s) 672, 1437
PctI GAATGC 2 cut(s) 804, 1421
PdmI GAANNNNTTC 1 cut(s) 1165
PfeI GAWTC 5 cut(s) 17, 205, 954, 1189, 1529
PkrI GCNGC 5 cut(s) 571, 649, 936, 1339, 1355
Ppu21I YACGTR 3 cut(s) 247, 1210, 1492
PscI ACATGT 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 1289
PsiI TTATAA 1 cut(s) 1382
Psp124BI GAGCTC 1 cut(s) 1560
PspFI CCCAGC 1 cut(s) 1194
PspN4I GGNNCC 2 cut(s) 1051, 1062
PspPI GGNCC 1 cut(s) 1060
PstI CTGCAG 3 cut(s) 652, 1114, 1355
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 2 cut(s) 1300, 1507
RruI TCGCGA 1 cut(s) 952
RsaI GTAC 4 cut(s) 156, 249, 612, 1220
RsaNI GTAC 4 cut(s) 155, 248, 611, 1219
RseI CAYNNNNRTG 1 cut(s) 1471
SacI GAGCTC 1 cut(s) 1560
SacII CCGCGG 1 cut(s) 1514
SapI GCTCTTC 2 cut(s) 835, 1548
SaqAI TTAA 4 cut(s) 194, 363, 1020, 1289
SatI GCNGC 5 cut(s) 570, 648, 935, 1338, 1354
Sau3AI GATC 8 cut(s) 66, 387, 399, 534, 788, 1300, 1310, 1507
Sau96I GGNCC 1 cut(s) 1060
ScrFI CCNGG 2 cut(s) 1064, 1298
SduI GDGCHC 4 cut(s) 506, 1209, 1378, 1560
SfaNI GCATC 5 cut(s) 103, 903, 1047, 1053, 1250
SfcI CTRYAG 4 cut(s) 648, 1110, 1351, 1401
Sfr303I CCGCGG 1 cut(s) 1514
SgrBI CCGCGG 1 cut(s) 1514
SinI GGWCC 1 cut(s) 1060
SmiMI CAYNNNNRTG 1 cut(s) 1471
SmlI CTYRAG 2 cut(s) 467, 544
SmoI CTYRAG 2 cut(s) 467, 544
SphI GCATGC 1 cut(s) 1476
Sse9I AATT 9 cut(s) 83, 180, 344, 495, 510, 726, 792, 1286, 1383
SsiI CCGC 4 cut(s) 602, 1365, 1511, 1513
SspMI CTAG 5 cut(s) 144, 309, 540, 938, 1358
SstI GAGCTC 1 cut(s) 1560
StyD4I CCNGG 2 cut(s) 1062, 1296
TaaI ACNGT 2 cut(s) 169, 946
TaiI ACGT 3 cut(s) 249, 1212, 1494
TaqI TCGA 5 cut(s) 208, 730, 1169, 1293, 1532
TasI AATT 9 cut(s) 83, 180, 344, 495, 510, 726, 792, 1286, 1383
TatI WGTACW 1 cut(s) 610
TfiI GAWTC 5 cut(s) 17, 205, 954, 1189, 1529
Tru1I TTAA 4 cut(s) 194, 363, 1020, 1289
Tru9I TTAA 4 cut(s) 194, 363, 1020, 1289
TscAI CASTG 4 cut(s) 496, 536, 754, 853
TseFI GTSAC 1 cut(s) 1010
TseI GCWGC 5 cut(s) 569, 647, 934, 1337, 1353
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 6 cut(s) 394, 511, 868, 980, 1115, 1417
TspGWI ACGGA 2 cut(s) 787, 1070
TspRI CASTG 4 cut(s) 496, 536, 754, 853
VneI GTGCAC 1 cut(s) 1205
VpaK11BI GGWCC 1 cut(s) 1060
VspI ATTAAT 1 cut(s) 1289
XapI RAATTY 2 cut(s) 83, 180
XceI RCATGY 2 cut(s) 600, 1476
XmnI GAANNNNTTC 1 cut(s) 1165
XspI CTAG 5 cut(s) 144, 309, 540, 938, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.